Taxol protects the microtubules of concanavalin A-activated lymphocytes from disassembly by methylmercury, but DNA synthesis is still inhibited.

abstract：:Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...

journal_title：Experimental cell research

authors： Beurel E,Kornprobst M,Blivet-Van Eggelpoël MJ,Ruiz-Ruiz C,Cadoret A,Capeau J,Desbois-Mouthon C

更新日期：2004-11-01 00:00:00

abstract：:In culture, nontransformed human diploid fibroblasts divide a limited number of times, resulting in a nonproliferating senescent cell culture which exhibits an altered pattern of gene expression. Previously we reported that an early event in the process of replicative senescence was an increase in the synthesis of two...

journal_title：Experimental cell research

authors： Zeng G,McCue HM,Mastrangelo L,Millis AJ

更新日期：1996-11-01 00:00:00

abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

journal_title：Experimental cell research

authors： Grant NJ,Aimar C,Oriol-Audit C

更新日期：1984-02-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) support life-long spermatogenesis by self-renewing and producing spermatogonia committed to differentiation. In vitro, SSCs form three-dimensional spermatogonial aggregates (clusters) when cultured with glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (...

journal_title：Experimental cell research

authors： Ebata KT,Yeh JR,Zhang X,Nagano MC

更新日期：2011-06-10 00:00:00

abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

journal_title：Experimental cell research

authors： List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

更新日期：2000-10-10 00:00:00

abstract：:The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

journal_title：Experimental cell research

authors： Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

更新日期：1983-12-01 00:00:00

abstract：:We have recently demonstrated that thrombin-activated FXIII (FXIIIA-subunit), a plasma transglutaminase, activates VEGFR-2 by crosslinking it with the alpha(v)beta(3) integrin on the surface of endothelial cells (EC), thereby stimulating angiogenesis. Tissue transglutaminase (tTG), which is functionally and structural...

journal_title：Experimental cell research

authors： Dardik R,Inbal A

更新日期：2006-10-01 00:00:00

abstract：:Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conv...

journal_title：Experimental cell research

authors： Hinz B,McCulloch CA,Coelho NM

更新日期：2019-06-01 00:00:00

abstract：:It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...

journal_title：Experimental cell research

authors： Tabellini G,Bortul R,Santi S,Riccio M,Baldini G,Cappellini A,Billi AM,Berezney R,Ruggeri A,Cocco L,Martelli AM

更新日期：2003-07-01 00:00:00

abstract：:Primary cultures of human keratinocytes form a multilayered tissue. By incubating the tissue cultures in Ca2(+)-free medium the differentiated cell layers can be stripped off leaving a basal cell monolayer. We have developed a method for high-frequency transfection of these epidermal basal cells with genes inserted in...

journal_title：Experimental cell research

authors： Jensen TG,Jensen PK,Nørgård JO,Bolund L

更新日期：1990-08-01 00:00:00

abstract：:Treatment of NRK cells with TGF-alpha in the presence of serum initiates disassembly of cytoskeletal stress fibers and suppresses the synthesis of tropomyosin isoforms (TMs) 1, 2, and 3 but not TMs 4 and 5 (Cooper et al., Cancer Res. 47, 4493-4500, 1987). In order to determine how the loss of tropomyosin is induced an...

journal_title：Experimental cell research

authors： Warren RH

更新日期：1997-10-10 00:00:00

abstract：:A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' c...

journal_title：Experimental cell research

authors： Schonberg S,Patterson D,Puck TT

更新日期：1983-04-15 00:00:00

abstract：:The poor outcome of hepatocellular carcinoma (HCC) is mainly due to the development of fast growth, invasion and metastasis. The role of TET2 has been implicated in some cancer types, but its role and mechanisms in HCC remains elusive. In this study, our findings indicated that TET2 expression frequently increased in ...

journal_title：Experimental cell research

authors： Yang G,Zeng X,Wang M,Wu A

更新日期：2018-02-15 00:00:00

abstract：:The addition of arachidonic acid (20:4), but not other fatty acids, including the structurally similar eicosapentaenoic acid (20:5), induced specific morphological changes in cultured endothelial cells derived from bovine aorta and pulmonary artery. Cells exhibited a time- and dose-dependent change from their normal, ...

journal_title：Experimental cell research

authors： Sandra A,Bar RS,Dolash S,Marshall SJ,Kaduce TL,Spector AA

更新日期：1985-06-01 00:00:00

abstract：:We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...

journal_title：Experimental cell research

authors： Udy GB,Parkes BD,Wells DN

更新日期：1997-03-15 00:00:00

abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...

journal_title：Experimental cell research

authors： Huang Y,Wang JJ,Ghosh S,Liu JL

更新日期：2017-05-15 00:00:00

abstract：:Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...

journal_title：Experimental cell research

authors： Fedorov YV,Prudovsky IA,Yegorov YE,Zelenin AV

更新日期：1993-11-01 00:00:00

abstract：:In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7 beta), which is composed of 333 amino acids. CeGLC-7 beta po...

journal_title：Experimental cell research

authors： Sassa T,Ueda-Ohba H,Kitamura K,Harada S,Hosono R

更新日期：2003-07-15 00:00:00

abstract：:A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transf...

journal_title：Experimental cell research

authors： Ohno T,Ohkawa A,Sato C

更新日期：1985-06-01 00:00:00

abstract：:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...

journal_title：Experimental cell research

authors： Hamilton BT,Snyder JA

更新日期：1983-04-01 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：:Hispidin, a phenolic compound from Phellinus linteus (a medicinal mushroom), has been shown to possess strong anti-oxidant, anti-cancer, anti-diabetic, and anti-dementia properties. However, the cardioprotective efficacy of hispidin has not yet been investigated. In the present study, we investigated the protective ef...

journal_title：Experimental cell research

authors： Kim DE,Kim B,Shin HS,Kwon HJ,Park ES

更新日期：2014-10-01 00:00:00

abstract：:The possible role of transferrin receptors in the diacytosis of human asialotransferrin type 3 (HAsTf-3) by the rat liver was studied in vivo. A trace dose of the ligand was allowed to compete for hepatic binding sites against diferric transferrin, the concentration of which was varied between 5 400- and 18 000-fold. ...

journal_title：Experimental cell research

authors： Regoeczi E,Koj A

更新日期：1985-09-01 00:00:00

abstract：:The biosynthesis of type XI and type II collagens was examined using a stable rat chondrocyte cell line established by W. E. Horton et al. (1988, Exp. Cell Res. 178, 457-468.). These cells (IRC; immortalized rat chondrocytes) were created by transformation with a murine retrovirus carrying the v-myc and v-raf oncogene...

journal_title：Experimental cell research

authors： Oxford JT,Doege KJ,Horton WE Jr,Morris NP

更新日期：1994-07-01 00:00:00

abstract：:Neurons require adhesive scaffolds for their growth and differentiation. Laminins are a major cell adhesive component of basement membranes and have various biological activities in the peripheral and central nervous systems. Here, we evaluated the biological activities of 5 peptides derived from laminin-111 as a scaf...

journal_title：Experimental cell research

authors： Hayashi H,Horinokita I,Yamada Y,Hamada K,Takagi N,Nomizu M

更新日期：2020-12-22 00:00:00

abstract：:12-Lipoxygenase, an arachidonic acid metabolizing enzyme of the lipoxygenase pathway, has been implicated as a major factor in promoting prostate cancer progression and metastasis. The ability of 12-LOX to aggravate the disease was linked to its proangiogenic role. Recent studies clearly demonstrated that 12-LOX enhan...

journal_title：Experimental cell research

authors： Krishnamoorthy S,Jin R,Cai Y,Maddipati KR,Nie D,Pagès G,Tucker SC,Honn KV

更新日期：2010-06-10 00:00:00

abstract：:The double-stranded RNA-dependent kinase, PKR, is encoded by an interferon inducible gene and is largely responsible for the anti-viral effects of this cytokine. Recent studies have shown that PKR may also play a role in the regulation of normal cellular growth. Although numerous examples of viral strategies for inact...

journal_title：Experimental cell research

authors： Abraham N,Jaramillo ML,Duncan PI,Méthot N,Icely PL,Stojdl DF,Barber GN,Bell JC

更新日期：1998-11-01 00:00:00

abstract：:The loss of expression of a single gene can revert normal tissue to a malignant phenotype. For example, while normal breast has high lumenal expression of CEACAM1, the majority of breast cancers exhibit the early loss of this gene with the concurrent loss of their lumenal phenotype. MCF7 cells that lack CEACAM1 expres...

journal_title：Experimental cell research

authors： Chean J,Chen CJ,Shively JE

更新日期：2017-10-01 00:00:00

abstract：:In the past, a unique type of precursor for a secretory protein was discovered. It contains a central repetitive domain rich in threonine residues and terminal cysteine-rich domains. Due to striking homologies of these terminal domains with pancreatic spasmolytic polypeptide, originally the name "prepro-spasmolysin" w...

journal_title：Experimental cell research

authors： Hauser F,Gertzen EM,Hoffmann W

更新日期：1990-08-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00