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High-frequency transfection of cultured human epidermal basal cells that differentiate to form a multilayered tissue.

Abstract：

:Primary cultures of human keratinocytes form a multilayered tissue. By incubating the tissue cultures in Ca2(+)-free medium the differentiated cell layers can be stripped off leaving a basal cell monolayer. We have developed a method for high-frequency transfection of these epidermal basal cells with genes inserted into Epstein-Barr virus-based expression vectors. Using the Escherichia coli lac z gene as a marker gene, the transient and long-term expression and the fate of the transfected cells were studied. During regeneration of the multilayered tissue most of the transfected basal cells enlarge and undergo differentiation, but a minor population remains as basal cells. Incubation with the tumor promotor 12-O-tetradecanoylphorbol-13-acetate results in an increase in the proportion of transfected keratinocytes that are small, suggesting a relative expansion of the immature cell pool.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Jensen TG,Jensen PK,Nørgård JO,Bolund L

doi

10.1016/0014-4827(90)90231-x

subject

Has Abstract

pub_date

1990-08-01 00:00:00

pages

163-8

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

189

pub_type

杂志文章

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