Abstract：
:Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces apoptosis through ER Ca2+ depletion and ER-specific pathways. ER stress and the disorder of intracellular calcium trigger the apoptosis induced by ASY overexpression. At the same time, stable transfectants overexpressing high levels of ASY are resistant to ER-stress-associated stimuli, which implies that ASY overexpression activates protective response in response to ER stress. Our results provide a direct apoptotic pathway that ASY overexpression induces apoptosis through ER stress and ER-specific signal pathways.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Kuang E,Wan Q,Li X,Xu H,Zou T,Qi Ydoi
10.1016/j.yexcr.2006.02.024subject
Has Abstractpub_date
2006-07-01 00:00:00pages
1983-8issue
11eissn
0014-4827issn
1090-2422pii
S0014-4827(06)00084-Xjournal_volume
312pub_type
杂志文章abstract：:Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conv...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2019.03.027
更新日期：2019-06-01 00:00:00
abstract：:Inverted thyroid follicles in suspension culture were observed using dark-field illumination and time-lapse video enhancement techniques. The lumens of many follicles undergo episodes of rapid shrinkage followed by relatively slow dilation. The extent of shrinkage ranged from 10 to 40% of the initial diameter. The dec...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90447-7
更新日期：1986-01-01 00:00:00
abstract：:Translation is mediated partly by regulation of free eukaryotic initiation factor 4E (eIF4E) levels through PI3K-Akt-mTOR signaling. Cancer cells treated with the plant-derived perillyl alcohol (POH) or the mechanistic target of rapamycin (mTOR) inhibitor rapamycin dephosphorylate eIF4E-binding protein (4E-BP1) and at...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.05.029
更新日期：2013-08-01 00:00:00
abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2012.02.026
更新日期：2012-05-15 00:00:00
abstract：:Psoriasis is a chronic inflammatory skin disease severely affecting patients' physical and psychological well-being. Aberrant keratin expression in psoriasis plays a crucial role in keratinocyte dysfunction and disease development. Little is yet known about the mechanism of this keratin dysregulation. VEGF (Vascular e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.09.021
更新日期：2017-11-15 00:00:00
abstract：:The radula of the limpet, Cellana toreuma, consists of a continuous series of teeth in various stages of iron biomineralization. The major iron-binding protein of the limpet's iron-containing granule (siderosome) has been purified and identified as ferritin. Limpet ferritin has a M(r) of 575 kDa and can be resolved in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1214
更新日期：1995-07-01 00:00:00
abstract：:This study was designed to elucidate the mechanisms involved in elevated cell death arising from an altered endogenous oxidant state. Increased levels of cell death were detected in cells lacking Gpx1 following the addition of exogenous H2O2. This increased apoptosis correlated with a down-regulation in the activation...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.07.018
更新日期：2004-11-01 00:00:00
abstract：:p107 and p130 were originally identified as targets of the transforming domains of viral oncoproteins encoded by small DNA tumor viruses. Together with pRB, the protein product of the retinoblastoma gene (Rb), p107 and p130 represent a family of closely related proteins that play critical roles in the regulation of ce...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.2000.5135
更新日期：2001-03-10 00:00:00
abstract：:Melanotransferrin is a glycoprotein expressed at the cell membrane and secreted in the extracellular environment. Recombinant truncated form of membrane-bound melanotransferrin (sMTf) was reported to exert in vitro anti-angiogenic properties. Here we show that sMTf treatment leads to a 50% inhibition of neovasculariza...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.04.013
更新日期：2007-08-01 00:00:00
abstract：:The presence of about 1.2 M glycerol during transfection with DNA/transferrin-polylysine and DNA/polylysine complexes dramatically increases transgene expression in a variety of cell types, provided that the complexes have an excess of polylysine. We have characterized this phenomenon using a human melanoma cell line ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3486
更新日期：1997-04-10 00:00:00
abstract：:There is at present a controversy in the literature whether MSCs are susceptible to spontaneous in vitro transformation or not. Several groups have reported spontaneous transformation of MSCs from various species. However, some of these reports were not true transformations and later proven to be due to cross-contamin...
journal_title：Experimental cell research
pub_type： 评论,杂志文章
doi：10.1016/j.yexcr.2011.12.009
更新日期：2012-03-10 00:00:00
abstract：:Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possib...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3916
更新日期：1998-03-15 00:00:00
abstract：:In primary cultures of ovine thyroid cells, TSH induced the expression of several differentiated functions including the formation of follicles, and synthesis and storage of iodinated thyroglobulin in the follicular lumen. In the present report, these follicles were shown by transmission (TEM) and scanning electron mi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90031-5
更新日期：1986-06-01 00:00:00
abstract：:Mps-one-binder (Mob) proteins play a crucial role in yeast cytokinesis. After cloning two Mob1-like genes, MsMob1-A and MsMob1-B from alfalfa (Medicago sativa L.) we show that, although they are constitutively expressed in roots, stems, leaves, flowers and pods, their transcripts and proteins are mostly produced in ac...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.032
更新日期：2006-04-15 00:00:00
abstract：:The high level and intranuclear location of the cellular phosphoprotein p53 are usually regarded as invariant features of SV40-transformed fibroblast lines. During the development of improved methods for immunocytochemical detection of p53 using SVA31 E7 mouse fibroblasts, we have observed unexpected and marked variat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90510-0
更新日期：1986-09-01 00:00:00
abstract：:Alexander disease (AxD) is a rare neurodegenerative disorder characterized by large cytoplasmic aggregates in astrocytes and myelin abnormalities and caused by dominant mutations in the gene encoding glial fibrillary acidic protein (GFAP), the main intermediate filament protein in astrocytes. We tested the effects of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.04.035
更新日期：2007-08-01 00:00:00
abstract：:Previous work has suggested that adhesive or cytoskeletal structures alter during epithelial transformation. The purpose of the present studies was to see whether such reorganizations were accompanied by consistent changes in the biophysical characteristics of cells. We used in vitro cell lines from rat liver and resp...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90232-1
更新日期：1987-03-01 00:00:00
abstract：:Products of the Neuregulin-1 (Nrg-1) gene, along with the ErbB family of receptor tyrosine kinases through which Nrg-1 ligands signal, play a critical role during cardiovascular development. Through studies of genetically manipulated mice, as well as studies in cells isolated from adult hearts, it appears that Nrg-1/E...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2008.08.015
更新日期：2009-02-15 00:00:00
abstract：:The p53-regulated stress-inducible gene GADD45 has been shown to participate in cellular response to DNA damage, including cell cycle checkpoint, apoptosis, and DNA repair. However, the regulation of GADD45 expression is complex and may involve both p53-dependent and -independent pathways. Recent findings have demonst...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5312
更新日期：2001-09-10 00:00:00
abstract：:After epithelial disruption by tissue injury, keratinocytes migrate from the wound edge into a provisional matrix. This process is stimulated by growth factors that signal through epidermal growth factor (EGF) receptor, including EGF, heparin-binding EGF-like growth factor (HB-EGF) and transforming growth factor-alpha...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.05.009
更新日期：2006-09-10 00:00:00
abstract：:A monoclonal antibody Pr-28 was prepared, which recognized an antigen present only in proliferating cells. Immunofluorescence analysis of Pr-28 antigen showed that the antigen was localized mainly in perinuclear cytoplasm. Although Pr-28 antibody was produced against a chicken cell antigen, it reacts not only with chi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90312-0
更新日期：1987-06-01 00:00:00
abstract：:The NADH-linked substrates pyruvate, L-glutamine, and beta-hydroxybutyrate, while enhancing the rate of oxygen consumption, also increased the formation of DNA single-strand breaks induced by tert-butylhydroperoxide in intact U937 cells. A cause-effect relationship between these two parameters was established by showi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3779
更新日期：1997-11-25 00:00:00
abstract：:Mesenchymal stem cells (MSCs) secrete paracrine factors that could be cytoprotective and serve roles in immunoregulation during tissue injury. Although MSCs express HIV receptors, and co-receptors, and are susceptible to HIV infection, whether HIV-1 may affect biological properties of MSCs needs more study. We evaluat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.06.008
更新日期：2013-08-15 00:00:00
abstract：:In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90117-0
更新日期：1987-05-01 00:00:00
abstract：:The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90034-6
更新日期：1992-11-01 00:00:00
abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5018
更新日期：2000-10-10 00:00:00
abstract：:A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90438-z
更新日期：1991-12-01 00:00:00
abstract：:During ovarian folliculogenesis granulosa cells divide while in contact with stromal cells and other granulosa cells. Following ovulation, however, they cease dividing and differentiate into large luteal cells. When cultured in monolayer, granulosa cells spontaneously differentiate into luteal cells, thus confounding ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1084
更新日期：1994-04-01 00:00:00
abstract：:We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underw...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1193
更新日期：1993-08-01 00:00:00
abstract：:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90288-7
更新日期：1989-05-01 00:00:00
