Abstract:
:Identification of breast cancer not being a single disease but backed by multiple heterogeneous oncogenic subpopulations is of growing interest in developing personalized therapies to provide optimal outcomes. Through this review, we bring attention to evolution of tumor and microenvironment heterogeneity as a predominant challenge in stratifying therapies. Establishment of a 'precancer niche' serves as a prerequisite for genetically initiated cells to survive and promote neoplastic evolution towards clinically established cancer through development of tumor and its microenvironment. Additionally, continuous evolutionary interplay between tumor and recruited stromal cells along with many other components in the tumor microenvironment adds up to further complexity in developing targeted therapies. However, through continued excellence in developing high throughput technologies including the advent of single-nucleus sequencing, which makes it possible to sequence individual tumor cells, leads to improved abilities in decoding the heterogenic perturbations through reconstruction of tumor evolutionary lineages. Furthermore, simple liquid-biopsies in form of enumeration/characterization of circulating tumor cells and tumor microvesicles found in peripheral circulation, shed from distinct tumor lesions, show great promise as prospective biomarkers towards better prognosis in tailoring individualized therapies to breast cancer patients. Lastly, by means of network medicinal approaches, it is seemingly possible to develop a map of the cell's intricate wiring network, helping to identify appropriate interconnected protein networks through which the disease spreads, offering a more patient-specific outcome. Although these therapeutic interventions through designing personalized oncology-based trials are promising, owing to continuous tumor evolution, targeting genome instability survival pathways might become an economically viable alternative.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Nandy A,Gangopadhyay S,Mukhopadhyay Adoi
10.1016/j.yexcr.2013.09.002subject
Has Abstractpub_date
2014-01-01 00:00:00pages
1-11issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(13)00384-4journal_volume
320pub_type
杂志文章,评审abstract::LMX1B is a LIM-homeodomain transcription factor essential for development. Putative LMX1B target genes have been identified through mouse gene targeting studies, but their identity as direct LMX1B targets remains hypothetical. We describe here the first molecular characterization of LMX1B target gene regulation. Micro...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.10.012
更新日期:2009-01-01 00:00:00
abstract::The effects of non-drug-containing liposomes of different compositions and sizes on the proliferation of nine cancer-derived and one normal cultured human cell lines were determined. Stearylamine- and cardiolipin-containing liposomes were toxic (ID50) at 200 microM liposomal lipid concentrations or less, whereas phosp...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90262-x
更新日期:1987-07-01 00:00:00
abstract::Subsets of glycoproteins are thought to require lectin-like membrane receptors for efficient export out of the endoplasmic reticulum (ER). To identify new members related to two previously characterized intracellular lectins ERGIC-53/p58 and VIP36, we carried out an extensive database search using the conserved carboh...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00161-7
更新日期:2003-08-01 00:00:00
abstract::Natural BH3-memitic (-)-gossypol shows promising antitumor efficacy in several kinds of cancer. However, our previous studies have demonstrated that protective autophagy decreases the drug sensitivities of Bcl-2 inhibitors in hepatocellular carcinoma (HCC) cells. In the present study, we are the first to report that H...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.08.039
更新日期:2014-11-01 00:00:00
abstract::Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.04.007
更新日期:2006-08-01 00:00:00
abstract::The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90034-6
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abstract::We investigated whether cell-permeable, synthetic ceramide (C6 ceramide) could induce apoptosis in Fas-resistant Hodgkin's disease (HD)-derived cell lines. Despite strongly expressing the Fas-receptor, two of three HD-derived cell lines were resistant to Fas-mediated apoptosis. This resistance to Fas could not be attr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4773
更新日期:2000-02-25 00:00:00
abstract::Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated nucleotides has been recently proposed as a means of analyzing DNA replication and detecting proliferating cells. The presence of numerous in situ DNA strand breaks is also an inherent feature of apoptotic cells. T...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0004
更新日期:1996-01-10 00:00:00
abstract::A method for the in vitro perfusion of isolated guinea-pig mammary tissue is described that allows the radiolabelling of secretory and membrane proteins. Glands were depleted of methionine, labelled with [35S]methionine for 5 min and perfused with medium containing an excess of unlabelled methionine for varying times....
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90369-0
更新日期:1984-03-01 00:00:00
abstract::Studies were carried out to test the idea that transforming growth factor beta (TGFbeta) stimulated fibroblast contraction of collagen matrices by different mechanisms depending on mechanical loading on the cells and matrix. Under mechanically unloaded conditions (floating matrices), TGFbeta stimulated contraction dir...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5445
更新日期:2002-02-15 00:00:00
abstract::O-GlcNAc transferase (OGT)-mediated modification of protein Ser/Thr residues with O-GlcNAc influences protein activity, similar to the effects of phosphorylation. The anti-apoptotic Akt1 is both activated by phosphorylation and modified with O-GlcNAc. However, the nature and significance of the Akt1 O-GlcNAc modificat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.04.014
更新日期:2008-07-01 00:00:00
abstract::The interphase nucleus is a structurally ordered, three-dimensional structure, in which specific chromatin domains occupy distinct spatial positions that can, in turn, be modified with changes in cell function. A fundamental goal in developmental neurobiology is the identification of mechanisms that dictate the orderl...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4793
更新日期:2000-04-10 00:00:00
abstract::The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ioni...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90557-9
更新日期:1985-02-01 00:00:00
abstract::Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5018
更新日期:2000-10-10 00:00:00
abstract::The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90709-2
更新日期:1984-01-01 00:00:00
abstract::We have used Xenopus egg extract to investigate the requirements for reactivation of DNA replication in nuclei isolated from terminally differentiated chicken erythrocytes. Previous work has shown that reactivation of erythrocyte nuclei in egg extract is accompanied by chromatin decondensation, nuclear envelope reform...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3520
更新日期:1997-05-01 00:00:00
abstract::ADP-ribosylation factors (ARFs) are modified by a myristate group that is covalently linked to the second glycine residue at the amino terminus. With the recent evidence that ARF6 localizes to the cell periphery and plays a regulatory role in endocytic traffic, we have investigated the role of myristoylation on the me...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1362
更新日期:1995-11-01 00:00:00
abstract::A discrete macromolecular structure with inherent transcription activity has been isolated from Novikoff nucleoli. The particle (350 A diameter) was released from the intact nucleoli by digestion with restriction endonuclease (Msp1) and is enriched in repetitive DNA sequences both 5' and 3' to the ribosomal genes. In ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90531-8
更新日期:1986-07-01 00:00:00
abstract::Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90216-w
更新日期:1990-01-01 00:00:00
abstract::Heat shock factor 1 (Hsf1) is an ancient transcription factor that monitors protein homeostasis (proteostasis) and counteracts disturbances by triggering a transcriptional programme known as the heat shock response (HSR). The HSR is transiently activated and upregulates the expression of core proteostasis genes, inclu...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2020.112246
更新日期:2020-11-01 00:00:00
abstract::The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90072-4
更新日期:1989-02-01 00:00:00
abstract::We have recently demonstrated that thrombin-activated FXIII (FXIIIA-subunit), a plasma transglutaminase, activates VEGFR-2 by crosslinking it with the alpha(v)beta(3) integrin on the surface of endothelial cells (EC), thereby stimulating angiogenesis. Tissue transglutaminase (tTG), which is functionally and structural...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.05.019
更新日期:2006-10-01 00:00:00
abstract::We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1098
更新日期:1993-04-01 00:00:00
abstract::Hepatoblastoma (HB) is a rare malignant embryonal liver tumor. Its pathogenesis has been associated with altered regulation of the IGF2 and H19 genes, and previous studies have suggested a correlation between abnormal methylation and altered expression of these genes in hepatoblastoma. Upregulation of the activity of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5336
更新日期:2001-10-15 00:00:00
abstract::Methylated amino acids inhibit lysosomal function in cultured rat heart myocytes more effectively than the classically employed lysosomotropic weak bases. Moreover, L-leucine methyl ester (L-Leu-OMe) or L-methionine methyl ester (L-Meth-OMe) do not alter lysosomal pH or inactivate lysosomal cysteine proteinases, but d...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90690-6
更新日期:1984-09-01 00:00:00
abstract::Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p5...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.06.006
更新日期:2010-09-10 00:00:00
abstract::Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.2000.5039
更新日期:2000-11-25 00:00:00
abstract::Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.3464
更新日期:1997-02-25 00:00:00
abstract::We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1115
更新日期:1994-05-01 00:00:00
abstract::A monoclonal antibody specific for vimentin is described which, by immunofluorescence and immunoelectron microscopy, decorates fibrillar and/or granular structures in mitotic and early postmitotic cells but does not react with vimentin filaments of interphase stages of various cultured cells (rat vascular smooth muscl...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90181-2
更新日期:1984-10-01 00:00:00