Abstract:
:Spermatogonial stem cells (SSCs) support life-long spermatogenesis by self-renewing and producing spermatogonia committed to differentiation. In vitro, SSCs form three-dimensional spermatogonial aggregates (clusters) when cultured with glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2); serial passaging of clusters results in long-term SSC maintenance and expansion. However, the role of these growth factors in controlling patterns of SSC division and fate decision has not been understood thoroughly. We report here that in a short-term culture, GDNF and FGF2 increase the number of dividing SSCs, but not the total SSC number, compared to a no-growth-factor condition. Since the total germ cell number increases with growth factors, these results suggest that GDNF and FGF2 promote a SSC division pattern that sustains the size of the stem cell pool while generating committed progenitors. Our data also show that SSC numbers increase when the cluster structure is disintegrated and cell-cell interaction in clusters is disrupted. Collectively, these results suggest that in this culture system, GDNF and FGF2 stimulate SSC divisions that promote self-renewal and differentiation in the SSC population, and imply that the destruction of the cluster structure, a potential in vitro niche, may contribute to SSC expansion.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ebata KT,Yeh JR,Zhang X,Nagano MCdoi
10.1016/j.yexcr.2011.03.013subject
Has Abstractpub_date
2011-06-10 00:00:00pages
1319-29issue
10eissn
0014-4827issn
1090-2422pii
S0014-4827(11)00105-4journal_volume
317pub_type
杂志文章abstract::The interfacial energy of the non-attached to substrate cell surface was analysed in tumor cell variants of the K-1735 melanoma and UV-2237 fibrosarcoma series, which exhibit distinct metastatic phenotypes. The highly metastatic cell variants exhibited a two-fold increase in the ability to form rapid cell substrate in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90625-6
更新日期:1984-08-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90278-g
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4663
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abstract::Activation of growth factor receptors, such as platelet-derived growth factor (PDGF) receptors, has a major impact on the motile behavior of vertebrate cells. The WASP family of proteins has been recognized as important regulators of actin polymerization via the activation of the Arp2/3 complex. The activity of the WA...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2002-09-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.3448
更新日期:1997-02-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3655
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90036-4
更新日期:1986-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1220
更新日期:1994-08-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3728
更新日期:1997-10-10 00:00:00
abstract::Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2000-11-25 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.04.020
更新日期:2013-08-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/0014-4827(85)90198-3
更新日期:1985-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2013.02.021
更新日期:2013-05-15 00:00:00
abstract::In order to test the existence of mechanical coupling between the rotational movements of two adjacent nuclei, we prepared binucleate 3T3 cells and observed their nuclear movements by near infrared microscopy and recorded them with time-lapse video techniques. We found that 49 out of 110 (44%) of the selected binuclea...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90512-4
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journal_title:Experimental cell research
pub_type: 杂志文章
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doi:10.1016/j.yexcr.2005.01.013
更新日期:2005-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4491
更新日期:1999-07-10 00:00:00
abstract::It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.05.010
更新日期:2017-08-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2003-11-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5163
更新日期:2001-04-15 00:00:00
abstract::Mechanical stress is an important regulator of chondrocyte functions but the mechanisms by which chondrocytes sense mechanical signals are unknown. Receptors for matrix molecules are likely involved in the mechanical signaling. In the first part of this study we identified integrins with affinity for the cartilage-spe...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1006/excr.2000.4943
更新日期:2000-08-01 00:00:00
abstract::Identification of transcription factors expressed by differentiated cells is informative not only of tissue-specific pathways, but to help identify master regulators for cellular reprogramming. If applied, such an approach could generate healthy autologous tissue-specific cells for clinical use where cells from the ho...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.05.031
更新日期:2018-08-15 00:00:00
abstract::A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90136-x
更新日期:1990-09-01 00:00:00