Abstract:
:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diameter. Application of colcemid, nocodazole, or vinblastine sulfate to cells at furrow initiation all increased the rate of furrowing. Nocodazole, applied at various concentrations, demonstrated a dose-dependent relationship with furrowing rate. These results suggest a coupling between the disorganization and depolymerization of microtubules and the acceleration of furrowing. Electron microscopic analysis of cells treated with microtubule inhibitors show approx. 70% fewer microtubule profiles in the constricted region between the daughter cells. Microtubules may play a restraining role in the rate of furrowing.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Hamilton BT,Snyder JAdoi
10.1016/0014-4827(83)90413-5subject
Has Abstractpub_date
1983-04-01 00:00:00pages
345-51issue
2eissn
0014-4827issn
1090-2422pii
0014-4827(83)90413-5journal_volume
144pub_type
杂志文章abstract::A novel fluorescence-based method was developed to measure HIV envelope glycoprotein (env)-CD4-mediated cell fusion. This method measures the spread of a fluorescent dye as the cytosolic compartments of adjacent cells become contiguous upon cell-to-cell fusion. Calcein-labeled CD4+ Sup-T1 cells were seeded onto a mono...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.3939
更新日期:1998-04-10 00:00:00
abstract::Hypoxia can activate the endothelium toward a pro-inflammatory phenotype and enhance leukocyte adhesion. This process is involved in pathological conditions such as vascular remodeling or ischemia-reperfusion injury. This study was aimed to obtain a global picture of the response of the endothelial cells to hypoxia wi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.11.020
更新日期:2009-03-10 00:00:00
abstract::Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (7...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90084-9
更新日期:1985-12-01 00:00:00
abstract::Giant axonal neuropathy skin fibroblasts, which are characterized by a selective and partial disorganization of vimentin filaments [1] exhibited, when compared with normal skin fibroblasts, less fibrin clot retractile (FCR) activity and spreading within the fibrin clot both during active growth and resting stage. Skin...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90794-8
更新日期:1984-11-01 00:00:00
abstract::Although there have been many reports on the relationship between activation of telomerase and carcinogenesis, the role of telomerase in normal cellular growth is still unclear. In this study, we analyzed the relationship between upregulation of telomerase activity and cell cycle progression during the liver regenerat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5446
更新日期:2002-02-15 00:00:00
abstract::We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90470-s
更新日期:1992-03-01 00:00:00
abstract::The effect of the cyclin-dependent (CDK) inhibitors olomoucine and roscovitine on cell kinetics was studied. To this end, nonsmall cell lung cancer (NSCLC) cell line MR65 and neuroblastoma cell line CHP-212 were pulse labeled with bromodeoxyuridine (BrdUrd) and chased in culture medium, to which various concentrations...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3700
更新日期:1997-10-10 00:00:00
abstract::Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.04.002
更新日期:2014-06-10 00:00:00
abstract::Raver2 was originally identified as a member of the hnRNP family through database searches revealing three N-terminal RNA recognition motifs (RRMs) bearing highest sequence identity in the RNP sequences to the related hnRNP Raver1. Outside the RRM region, both Raver proteins are quite divergent in sequence except for ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.11.023
更新日期:2010-04-01 00:00:00
abstract::The 37 kDa/67 kDa laminin receptor (LRP/LR) is over-expressed in tumor cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.04.003
更新日期:2018-07-01 00:00:00
abstract::Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90105-4
更新日期:1991-04-01 00:00:00
abstract::The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The re...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1016
更新日期:1993-01-01 00:00:00
abstract::Inflammatory mediators including inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) contribute to the course of a variety of inflammatory diseases. S632A3 is a new member of the glutarimide antibiotics isolated from a cultured broth of Streptomyces hygrosc...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.08.008
更新日期:2012-12-10 00:00:00
abstract::Whey Acidic Protein (WAP) gene expression is specific to the mammary gland and regulated by lactogenic hormones to peak during lactation. It differs markedly from the more constitutive expression of the two flanking genes, Ramp3 and Tbrg4. Our results show that the tight regulation of WAP gene expression parallels var...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.01.001
更新日期:2008-03-10 00:00:00
abstract::In a previous study we have shown that triiodothyronine (T3) added to a serum-free medium supplemented with insulin, transferrin, and selenous acid (ITS) can stimulate Caco-2 cell differentiation. In this study we have focused on the effects of T3 on sucrase activity. The results obtained demonstrate that T3 (50 nM) d...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0041
更新日期:1996-02-01 00:00:00
abstract::Uterine fibroids, also known as uterine leiomyomas, are a benign tumor of the human uterus and the commonest estrogen-dependent benign tumor found in women. Myocardin is an important transcriptional regulator in smooth and cardiac muscle development. The role of myocardin and its relationship with ERα in uterine fibro...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.11.007
更新日期:2017-01-01 00:00:00
abstract::SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90692-x
更新日期:1984-09-01 00:00:00
abstract::Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.08.021
更新日期:2016-10-15 00:00:00
abstract::Chronic lymphocytic leukemia lymphocytes were used to study mechanisms involved in apoptosis (programmed cell death). Apoptosis, which was determined by morphological changes including cell death and by internucleosomal DNA fragmentation, occurred during culture for 1 to 2 days in a portion of the cells from three of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90393-m
更新日期:1992-02-01 00:00:00
abstract::We previously showed that clonable skeletal myogenic cells can be derived from the embryonic aorta but become very rare in the more mature and structured fetal aorta. The aim of this study was to investigate whether, during fetal and postnatal development, these myogenic progenitors progressively disappear or may rath...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00314-8
更新日期:2003-11-01 00:00:00
abstract::Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.01.017
更新日期:2016-06-10 00:00:00
abstract::The insoluble cytoskeletal material remaining after detergent lysis of 'Normal' Murine Mammary Gland (NMuMG) cells, growing on plastic or collagen gel substrata, was analyzed by two-dimensional gel electrophoresis. The identity of the cytoskeletal elements was determined by their solubility properties, electrophoretic...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90343-5
更新日期:1986-02-01 00:00:00
abstract::Alzheimer's disease (AD) is characterized by early degeneration of cholinergic neurons and decreased levels of nerve growth factor (NGF). Thus, increasing the NGF levels by for instance encapsulated cell bio-delivery (ECB) is a potential treatment strategy. The results from our previous first-in-human studies on ECB o...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.08.007
更新日期:2018-10-01 00:00:00
abstract::The release of cell surface-derived microparticles, or ectosomes, has now been described for many different cell types. In various diseases characterized by systemic inflammation, the numbers of ectosomes released from specific cell-types are found increased manifold in the circulation. Their pro-inflammatory and pro-...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.03.011
更新日期:2005-07-15 00:00:00
abstract::Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.01.025
更新日期:2018-03-01 00:00:00
abstract::The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.02.028
更新日期:2014-05-01 00:00:00
abstract::We have investigated the genetic stability of NIH-3T3 cells transfected with sequences coding for basic fibroblast growth factor (bFGF) by determining drug resistance and gene amplification potential. Colony-forming experiments and fluctuation analyses showed that the frequency and rate of resistance to N-(phosphonace...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1207
更新日期:1994-08-01 00:00:00
abstract::The growth factor requirement of normal and polyomavirus middle T gene transformed REF52 cells was studied in serum-free medium in an attempt to elucidate the possible linkage between an altered growth factor requirement and one or more altered physiological properties of the transformed cells. For optimal growth, REF...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90432-1
更新日期:1989-07-01 00:00:00
abstract::Human cyclin D1 forms complexes with several Cdks, with proliferating cell nuclear antigen, and with a recently discovered 21-kDa inhibitor of Cdk activity. Substrates for cyclin D1/Cdks have not been identified in vivo, but it has been proposed that the D class of cyclins might play a role in regulating the activity ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1080
更新日期:1995-04-01 00:00:00
abstract::Liver cells isolated from the adult rat livers under mild conditions were preincubated for 1 day with Williams medium E (WE) containing serum, dexamethasone and insulin, and then the cells (monolayered) were incubated for 2-3 days with WE (1 ml) containing only insulin to measure DNA synthesis and/or mitosis. DNA synt...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90196-4
更新日期:1984-12-01 00:00:00