The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics.

abstract：:The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...

journal_title：Experimental cell research

authors： Marshall H,Martens I,Svensson C,Akusjärvi G,Linder S

更新日期：1991-05-01 00:00:00

abstract：:Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...

journal_title：Experimental cell research

authors： Kuang E,Wan Q,Li X,Xu H,Zou T,Qi Y

更新日期：2006-07-01 00:00:00

abstract：:The phosphorylation state of the CD18-chain of beta 2-integrins have been shown not to mediate changes in the avidity of these receptors (i.e., inside-out signaling); however, no alternative functional significance has been proposed. Our study focused on how changes in the phosphorylation state of beta 2-integrin-rece...

journal_title：Experimental cell research

authors： Hellberg C,Eierman D,Sjölander A,Andersson T

更新日期：1995-03-01 00:00:00

abstract：:Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, ...

journal_title：Experimental cell research

authors： Clawson GA,Friend DS,Smuckler EA

更新日期：1984-11-01 00:00:00

abstract：:NIH3T3 cells carrying a dominant negative H-ras mutant 116Y acquired resistance to transformation by some PTK oncogenes, i.e., v-fes, v-abl, and v-fms, but were sensitive to viral ras and serine threonine kinase oncogenes, v-raf and v-mos. One clone, designated 1-20, infected with v-fes (1-20 fes) exhibited flat morph...

journal_title：Experimental cell research

authors： Ogiso Y,Yokoyama T,Watari H,Shih TY,Kuzumaki N

更新日期：1993-10-01 00:00:00

abstract：:A novel fluorescence-based method was developed to measure HIV envelope glycoprotein (env)-CD4-mediated cell fusion. This method measures the spread of a fluorescent dye as the cytosolic compartments of adjacent cells become contiguous upon cell-to-cell fusion. Calcein-labeled CD4+ Sup-T1 cells were seeded onto a mono...

journal_title：Experimental cell research

authors： Pine PS,Weaver JL,Oravecz T,Pall M,Ussery M,Aszalos A

更新日期：1998-04-10 00:00:00

abstract：:We evaluated cellular mechanisms involved in the activation pathway of matrix prometalloproteinase-2 (pro-MMP-2), an enzyme implicated in the malignant progression of many tumor types. Membrane type-1 matrix metalloproteinase (MT1-MMP) cleaves the N-terminal prodomain of pro-MMP-2 thus generating the activation interm...

journal_title：Experimental cell research

authors： Deryugina EI,Ratnikov B,Monosov E,Postnova TI,DiScipio R,Smith JW,Strongin AY

更新日期：2001-02-15 00:00:00

abstract：:The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...

journal_title：Experimental cell research

authors： Folkman J

更新日期：2006-03-10 00:00:00

abstract：:Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

journal_title：Experimental cell research

authors： Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

更新日期：1994-08-01 00:00:00

abstract：:Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

journal_title：Experimental cell research

authors： De Wever B,Rooman RP,De Brabander M

更新日期：1990-01-01 00:00:00

abstract：:The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines. The three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. Bromodeoxyuridine becomes incorporated into the nuclear DNA and increases the spon...

journal_title：Experimental cell research

authors： Raskó I,Péter SL,Dallmann L,Bajszár G,Péter S,Burg K

更新日期：1978-05-01 00:00:00

abstract：:Incubation of cultured cells at 20 degrees C blocks the transport of newly synthesized plasma membrane proteins, and the proteins accumulate intracellularly in a terminally glycosylated form. When baby hamster kidney cells are infected with the ts O45 mutant of vesicular stomatitis virus, and incubated at 20 degrees C...

journal_title：Experimental cell research

authors： de Curtis I,Howell KE,Simons K

更新日期：1988-04-01 00:00:00

abstract：:Integrins are heterodimeric transmembrane proteins that mediate substrate adhesion and migration but also the bidirectional transfer of information across the plasma membrane via their cytoplasmic domains. We addressed the question of whether the very short cytoplasmic tail of the alpha1 integrin subunit of alpha1beta...

journal_title：Experimental cell research

authors： Vossmeyer D,Kaufmann C,Löster K,Lucka L,Horstkorte R,Reutter W,Danker K

更新日期：2000-04-10 00:00:00

abstract：:The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...

journal_title：Experimental cell research

authors： Takakubo F,Dahl HH

更新日期：1992-03-01 00:00:00

abstract：:Both hypoxia and overexpression of the urokinase plasminogen activator receptor (uPAR) are associated with a poor clinical outcome in human cancers. Hypoxia has been shown to induce uPAR expression in breast cancer cells and to increase their invasion through Matrigel, a phenomenon which can be blocked using an anti-u...

journal_title：Experimental cell research

authors： Maity A,Solomon D

更新日期：2000-03-15 00:00:00

abstract：:In multiple myeloma, which commonly depends on interleukin 6, IL-6, survival signaling induced by this cytokine is largely mediated by activation of STAT3. Interferon alpha (IFNalpha) treatment of cell lines derived from multiple myeloma or of myeloma tumor cells ex vivo leads to apoptosis. In this study we demonstrat...

journal_title：Experimental cell research

authors： Thyrell L,Arulampalam V,Hjortsberg L,Farnebo M,Grandér D,Pokrovskaja Tamm K

更新日期：2007-11-15 00:00:00

abstract：:Coiled bodies (CBs) are structural constituents observed in nuclei of most eukaryotic cells. They usually occur in the nucleoplasm as well as in contact with the nucleolar surface. In this work we studied the hepatocyte nuclei of hibernating dormice in order to investigate possible modifications of CBs along the seaso...

journal_title：Experimental cell research

authors： Malatesta M,Zancanaro C,Martin TE,Chan EK,Amalric F,Lührmann R,Vogel P,Fakan S

更新日期：1994-04-01 00:00:00

abstract：:We generated four monoclonal antibodies (mAbs) specific for human alpha 3 integrin (VLA-3 alpha subunit). All of them were found to induce homotypic cell aggregation of HT1080 fibrosarcoma and SN12C renal carcinoma cells, both of which express high levels of alpha 3 integrin. The antibodies also induced the cell aggre...

journal_title：Experimental cell research

authors： Takeuchi K,Tsuji T,Hakomori S,Irimura T

更新日期：1994-03-01 00:00:00

abstract：:Ring finger protein 6 (RNF6), a RING-domain E3 ubiquitin Ligase, functions as a potential tumor promoter in several caners. However, the function and clinical significance of RNF6 in hepatocellular carcinoma (HCC) remains unclear. Here, we found that high RNF6 expression was detected frequently in primary HCC tissues,...

journal_title：Experimental cell research

authors： Cai J,Xiong Q,Jiang X,Zhou S,Liu T

更新日期：2019-01-01 00:00:00

abstract：:The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...

journal_title：Experimental cell research

authors： Grøvdal LM,Johannessen LE,Rødland MS,Madshus IH,Stang E

更新日期：2008-04-01 00:00:00

abstract：:Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

journal_title：Experimental cell research

authors： Yamamoto K,Aoyagi M,Yamamoto M

更新日期：1995-05-01 00:00:00

abstract：:The results of Phase I/II clinical trials indicate that ricin A-chain-containing immunotoxins cause vascular leak syndrome, characterized by hypoalbuminemia with resultant weight gain and edema. Vascular leak syndrome may be a dose-limiting factor during treatment with ricin A-chain-containing immunotoxins. In this re...

journal_title：Experimental cell research

authors： Soler-Rodríguez AM,Ghetie MA,Oppenheimer-Marks N,Uhr JW,Vitetta ES

更新日期：1993-06-01 00:00:00

abstract：:Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins inclu...

journal_title：Experimental cell research

authors： Minato N

更新日期：2013-09-10 00:00:00

abstract：:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...

journal_title：Experimental cell research

authors： Wang J,Tolan DR,Pagliaro L

更新日期：1997-12-15 00:00:00

abstract：:L1 cell adhesion molecule (L1-CAM) is a transmembrane cell adhesion molecule initially defined as a promigratory molecule in the developing nervous system. L1 is also overexpressed in a variety of human carcinomas and is associated with bad prognosis. In carcinoma cell lines L1 augments cell motility and metastasis, t...

journal_title：Experimental cell research

authors： Gast D,Riedle S,Kiefel H,Müerköster SS,Schäfer H,Schäfer MK,Altevogt P

更新日期：2008-08-01 00:00:00

abstract：:CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...

journal_title：Experimental cell research

authors： Chen CJ,Nguyen T,Shively JE

更新日期：2010-02-15 00:00:00

abstract：:We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...

journal_title：Experimental cell research

authors： You N,Tan Y,Zhou L,Huang X,Wang W,Wang L,Wu K,Mi N,Li J,Zheng L

更新日期：2017-09-15 00:00:00

abstract：:Cell migration is part of many important in vivo biological processes and is influenced by chemical and physical factors such as substrate topography. Although the migratory behavior of different cell types on structured substrates has already been investigated, up to date it is largely unknown if specimen's age affec...

journal_title：Experimental cell research

authors： Sales A,Picart C,Kemkemer R

更新日期：2019-01-01 00:00:00

abstract：:We examined the relationship of cellular oncogene c-myc and transferrin receptor (TfR) gene expression to cell proliferation and cell cycle progression during myeloid differentiation in the HL-60 myeloid leukemia cell line. In order to determine levels of mRNA for these genes in HL-60 cells induced to differentiate al...

journal_title：Experimental cell research

authors： Barker KA,Newburger PE

更新日期：1990-01-01 00:00:00

abstract：:Previous studies showed nanosecond pulsed electric field (nsPEF) can ablate solid tumors including hepatocellular carcinoma (HCC) but its effect on cell membrane is not fully understood. We hypothesized nsPEF disrupt the microdomains on outer-cellular membrane with direct mechanical force and as a result the plasma me...

journal_title：Experimental cell research

authors： Yin S,Chen X,Xie H,Zhou L,Guo D,Xu Y,Wu L,Zheng S

更新日期：2016-08-15 00:00:00