Abstract:
:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, rather than a nonspecific mechanism, is responsible for localizing aldolase in vivo. In this report, we have used fluorescent analog cytochemistry of a site-directed mutant of aldolase to demonstrate that actin-binding activity localizes this molecule along stress fibers in quiescent cells and behind active ruffles in the leading edge of motile cells. The specific cytoskeletal association of aldolase could play a structural role in cytoplasm, and it may contribute to metabolic regulation, metabolic compartmentation, and/or cell motility. Functional duality may be a widespread feature among cytosolic enzymes.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Wang J,Tolan DR,Pagliaro Ldoi
10.1006/excr.1997.3811subject
Has Abstractpub_date
1997-12-15 00:00:00pages
445-51issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(97)93811-8journal_volume
237pub_type
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