Metabolic compartmentation in living cells: structural association of aldolase.

Abstract:

:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, rather than a nonspecific mechanism, is responsible for localizing aldolase in vivo. In this report, we have used fluorescent analog cytochemistry of a site-directed mutant of aldolase to demonstrate that actin-binding activity localizes this molecule along stress fibers in quiescent cells and behind active ruffles in the leading edge of motile cells. The specific cytoskeletal association of aldolase could play a structural role in cytoplasm, and it may contribute to metabolic regulation, metabolic compartmentation, and/or cell motility. Functional duality may be a widespread feature among cytosolic enzymes.

journal_name

Exp Cell Res

authors

Wang J,Tolan DR,Pagliaro L

doi

10.1006/excr.1997.3811

subject

Has Abstract

pub_date

1997-12-15 00:00:00

pages

445-51

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(97)93811-8

journal_volume

237

pub_type

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