Metabolic compartmentation in living cells: structural association of aldolase.


:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, rather than a nonspecific mechanism, is responsible for localizing aldolase in vivo. In this report, we have used fluorescent analog cytochemistry of a site-directed mutant of aldolase to demonstrate that actin-binding activity localizes this molecule along stress fibers in quiescent cells and behind active ruffles in the leading edge of motile cells. The specific cytoskeletal association of aldolase could play a structural role in cytoplasm, and it may contribute to metabolic regulation, metabolic compartmentation, and/or cell motility. Functional duality may be a widespread feature among cytosolic enzymes.


Exp Cell Res


Wang J,Tolan DR,Pagliaro L




Has Abstract


1997-12-15 00:00:00














  • Autocrine production of TGF-beta confers resistance to apoptosis after an epithelial-mesenchymal transition process in hepatocytes: Role of EGF receptor ligands.

    abstract::Transforming growth factor-beta (TGF-beta) induces apoptosis in fetal rat hepatocytes. However, a subpopulation of these cells survives, concomitant with changes in phenotype, reminiscent of an epithelial-mesenchymal transition (EMT). We have previously suggested that EMT might confer cell resistance to apoptosis (Val...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Del Castillo G,Murillo MM,Alvarez-Barrientos A,Bertran E,Fernández M,Sánchez A,Fabregat I

    更新日期:2006-09-10 00:00:00

  • Transforming growth factor beta stimulates fibroblast-collagen matrix contraction by different mechanisms in mechanically loaded and unloaded matrices.

    abstract::Studies were carried out to test the idea that transforming growth factor beta (TGFbeta) stimulated fibroblast contraction of collagen matrices by different mechanisms depending on mechanical loading on the cells and matrix. Under mechanically unloaded conditions (floating matrices), TGFbeta stimulated contraction dir...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grinnell F,Ho CH

    更新日期:2002-02-15 00:00:00

  • Bcl-2 prevents loss of mitochondria in CCCP-induced apoptosis.

    abstract::Bcl-2 family proteins regulate apoptosis at the level of mitochondria. To examine the mechanism of Bcl-2 function, we investigated the effects of the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) on two hematopoietic cell lines and Bcl-2 overexpressing transfectants. CCCP directly interferes with mitoc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: de Graaf AO,van den Heuvel LP,Dijkman HB,de Abreu RA,Birkenkamp KU,de Witte T,van der Reijden BA,Smeitink JA,Jansen JH

    更新日期:2004-10-01 00:00:00

  • Negative cooperativity between alpha 3 beta 1 and alpha 2 beta 1 integrins in human mammary carcinoma MDA MB 231 cells.

    abstract::The alpha 3 beta 1 integrin has been implicated as a receptor for several matrix components, including collagen, fibronectin, and laminins. The function of alpha 3 beta 1 seems to be very versatile involving cell adhesion to or migration on ECM, establishment of cell-cell contacts in aggregates, as well as linkage to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lichtner RB,Howlett AR,Lerch M,Xuan JA,Brink J,Langton-Webster B,Schneider MR

    更新日期:1998-05-01 00:00:00

  • The role of alpha3beta1 integrin in determining the supramolecular organization of laminin-5 in the extracellular matrix of keratinocytes.

    abstract::Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: deHart GW,Healy KE,Jones JC

    更新日期:2003-02-01 00:00:00

  • Genetic and epigenetic changes in clonal descendants of irradiated human fibroblasts.

    abstract::To study delayed genetic and epigenetic radiation effects, which may trigger radiation-induced carcinogenesis, we have established single-cell clones from irradiated and non-irradiated primary human fibroblasts. Stable clones were endowed with the same karyotype in all analyzed metaphases after 20 population doublings...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Flunkert J,Maierhofer A,Dittrich M,Müller T,Horvath S,Nanda I,Haaf T

    更新日期:2018-09-15 00:00:00

  • Sodium 4-phenylbutyrate upregulates ENaC and sodium absorption in T84 cells.

    abstract::Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Iordache C,Duszyk M

    更新日期:2007-01-15 00:00:00

  • OGDH mediates the inhibition of SIRT5 on cell proliferation and migration of gastric cancer.

    abstract::SIRT5 has a wide range of functions in different cellular processes such as glycolysis, TCA cycle and antioxidant defense, which mediates lysine desuccinylation, deglutarylation and demalonylation. Recent evidences have implicated that SIRT5 is a potential suppressor of gastric cancer (GC). However, the underlying mec...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lu X,Yang P,Zhao X,Jiang M,Hu S,Ouyang Y,Zeng L,Wu J

    更新日期:2019-09-15 00:00:00

  • Regulation of angiogenesis by PI3K signaling networks.

    abstract::Phosphoinositide 3-kinases (PI3Ks) are an evolutionary conserved family of lipid kinases that control cell growth, metabolism and survival. By generating lipid second messengers that interact with specialized lipid-binding domains found in a wide spectrum of signaling molecules, PI3Ks instigate signaling through a net...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Graupera M,Potente M

    更新日期:2013-05-15 00:00:00

  • An altered repertoire of fos/jun (AP-1) at the onset of replicative senescence.

    abstract::With multiple divisions in culture, normal diploid cells suffer a loss of growth potential that leads to replicative senescence and a finite replicative capacity. Using quantitative RT-PCR, we have monitored mRNA expression levels of c-fos, c-jun, JunB, c-myc, p53, H-ras, and histone H4 during the replicative senescen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Irving J,Feng J,Wistrom C,Pikaart M,Villeponteau B

    更新日期:1992-09-01 00:00:00

  • Type I collagen promotes modulation of cultured rabbit arterial smooth muscle cells from a contractile to a synthetic phenotype.

    abstract::The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The re...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yamamoto M,Yamamoto K,Noumura T

    更新日期:1993-01-01 00:00:00

  • Variations in signal-mediated nuclear transport during the cell cycle in BALB/c 3T3 cells.

    abstract::Signal-mediated nuclear import was investigated in proliferating BALB/c 3T3 cells 1, 2, 4, 6, 8, 12, 18, and 21 h after the onset of anaphase. Using nucleoplasmin-coated colloidal gold particles to assay transport capacity, it was found that import was greatest at 1 h postanaphase (after complete reformation of the nu...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Feldherr CM,Akin D

    更新日期:1994-11-01 00:00:00

  • S100P/RAGE signaling regulates microRNA-155 expression via AP-1 activation in colon cancer.

    abstract::Accumulating evidence indicates that elevated S100P promotes the pathogenesis of cancers, including colon cancer. S100P exerts its effects by binding to and activating the Receptor for Advance Glycation End-products (RAGE). The effects of up-regulated S100P/RAGE signaling on cell functions are well documented. Despite...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Onyeagucha BC,Mercado-Pimentel ME,Hutchison J,Flemington EK,Nelson MA

    更新日期:2013-08-01 00:00:00

  • A functional role for ERK in gene induction, but not in neurite outgrowth in differentiating neuroblastoma cells.

    abstract::The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Olsson AK,Nånberg E

    更新日期:2001-04-15 00:00:00

  • Specific binding of [3H]heparin to human carcinoma SW-13 and other mammalian cells.

    abstract::This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Halper J

    更新日期:1990-04-01 00:00:00

  • The nucleoside diphosphate kinase activity of DRnm23 is not required for inhibition of differentiation and induction of apoptosis in 32Dcl3 myeloid precursor cells.

    abstract::DRnm23 belongs to a multigene family which includes nm23-H1, the first bona fide metastasis suppressor gene, nm23-H2, nm23-H4, and nm23-H5. Like nm23-H1, nm23-H2, and nm23-H4, DRnm23 possesses nucleoside diphosphate kinase (NDPK) activity. Upon overexpression in myeloid precursor 32Dcl3 cells, DRnm23 inhibits granuloc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Venturelli D,Cesi V,Ransac S,Engelhard A,Perrotti D,Calabretta B

    更新日期:2000-06-15 00:00:00

  • Regulation of p53 in embryonic stem cells.

    abstract::Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p5...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Solozobova V,Blattner C

    更新日期:2010-09-10 00:00:00

  • Inhibition of the mitochondrial calcium uniporter prevents IL-13 and allergen-mediated airway epithelial apoptosis and loss of barrier function.

    abstract::Mitochondria are increasingly recognized as key mediators of acute cellular stress responses in asthma. However, the distinct roles of regulators of mitochondrial physiology on allergic asthma phenotypes are currently unknown. The mitochondrial Ca2+ uniporter (MCU) resides in the inner mitochondrial membrane and contr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sebag SC,Koval OM,Paschke JD,Winters CJ,Comellas AP,Grumbach IM

    更新日期:2018-01-15 00:00:00

  • Alteration in growth, cell morphology, and cytoskeletal structures of KB cells induced by epidermal growth factor and transforming growth factor-beta.

    abstract::Long-term biological effects of epidermal growth factor (EGF), insulin, insulin-like growth factor-I (IGF-I), and transforming growth factor-beta (TGF-beta) were examined with human epidermoid carcinoma KB cells. EGF inhibited the growth of KB cells in both serum-containing and serum-free synthetic media by reducing t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Koyasu S,Kadowaki T,Nishida E,Tobe K,Abe E,Kasuga M,Sakai H,Yahara I

    更新日期:1988-05-01 00:00:00

  • CD25-expressing Th17 cells mediate CD8+ T cell suppression in CTLA-4 dependent mechanisms in pancreatic ductal adenocarcinoma.

    abstract::The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lang C,Wang J,Chen L

    更新日期:2017-11-15 00:00:00

  • The role of scavenger receptor class A in the adhesion of cells is dependent on cell type and cellular activation state.

    abstract::Scavenger receptor class A (SR-A) facilitates the development of atherosclerosis, which might be due to its role in the uptake of modified low-density lipoproteins. However, the receptor is also suggested to be important for cell adhesion, thereby potentially influencing the residence time of cells in vivo. Using SR-A...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: van Velzen AG,Suzuki H,Kodama T,van Berkel TJ

    更新日期:1999-07-10 00:00:00

  • External Ca2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells.

    abstract::Polycystin-2 (PC2, TRPP2) is a nonselective cation channel whose dysfunction is associated with the onset of autosomal dominant polycystic kidney disease (ADPKD). PC2 contributes to Ca2+ transport and cell signaling in renal epithelia and other tissues. Little is known however, as to the external Ca2+ regulation of PC...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dai XQ,Perez PL,Soria G,Scarinci N,Smoler M,Morsucci DC,Suzuki K,Cantero MDR,Cantiello HF

    更新日期:2017-01-01 00:00:00

  • Tenascin-C in the extracellular matrix promotes the selection of highly proliferative and tubulogenesis-defective endothelial cells.

    abstract::The extracellular matrix (ECM) contains important cues for tissue homeostasis and morphogenesis. The matricellular protein tenascin-C (TN-C) is overexpressed in remodeling tissues and cancer. In the present work, we studied the effect of different ECM-which exhibited a significant diversity in their TN-C content-in en...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Alves TR,da Fonseca AC,Nunes SS,da Silva AO,Dubois LG,Faria J,Kahn SA,Viana NB,Marcondes J,Legrand C,Moura-Neto V,Morandi V

    更新日期:2011-09-10 00:00:00

  • Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells.

    abstract::In many animals, the germ line develops from a distinct mitochondria-rich region of embryonic cytoplasm called the germ plasm. However, the protein composition of germ plasm and its formation remain poorly understood, except in Drosophila. Here, we show that Xpat, a recently identified protein component of Xenopus ger...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hames RS,Hames R,Prosser SL,Euteneuer U,Lopes CA,Moore W,Woodland HR,Fry AM

    更新日期:2008-02-01 00:00:00

  • Human calmodulin-like protein is an epithelial-specific protein regulated during keratinocyte differentiation.

    abstract::Human calmodulin-like protein (CLP) is a calcium-binding protein down-regulated in a cell culture model of mammary tumorigenesis as well as in a majority of breast cancers in vivo. CLP down-regulation may be a result of the poorly differentiated state of these cell lines and tumors, or CLP expression may be incompatib...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rogers MS,Kobayashi T,Pittelkow MR,Strehler EE

    更新日期:2001-07-15 00:00:00

  • Myostatin signals through Pax7 to regulate satellite cell self-renewal.

    abstract::Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McFarlane C,Hennebry A,Thomas M,Plummer E,Ling N,Sharma M,Kambadur R

    更新日期:2008-01-15 00:00:00

  • Use of nonyl acridine orange and rhodamine 123 to follow biosynthesis and functional assembly of mitochondrial membrane during L1210 cell cycle.

    abstract::Specific mitochondrial incorporation of 10 N-nonyl acridine orange (NAO) is demonstrated by subcellular fractionation of rat hepatocytes. Moreover, comparative studies with NAO and rhodamine 123 (Rh 123) prove that acridine orange-derivative uptake is independent of transmembrane mitochondrial potential, a property al...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Leprat P,Ratinaud MH,Maftah A,Petit JM,Julien R

    更新日期:1990-01-01 00:00:00

  • Cylindrical substratum induces different patterns of actin microfilament bundles in nontransformed and in ras-transformed epitheliocytes.

    abstract::The influence of the cylindrical substratum surface on the actin microfilament bundle and the extracellular matrix (fibronectin and laminin) patterns in nontransformed epithelial cells of the IAR-2 rat line and in their N-ras-transformed descendants, IAR-ras-c4 cells, was studied. The cells were cultured on substrata ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Levina EM,Domnina LV,Rovensky YA,Vasiliev JM

    更新日期:1996-11-25 00:00:00

  • Selective proteolysis of the nuclear replication factor MCM3 in apoptosis.

    abstract::Cleavage of specific protein subsets is a key event in the execution of apoptosis. Protein degradation may serve for the structural alterations that result in cell self-destruction, but it may also function as a switch in the decisions between apoptosis and necrosis or apoptosis and cell proliferation. Here, we show t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schwab BL,Leist M,Knippers R,Nicotera P

    更新日期:1998-02-01 00:00:00

  • The blocked pinocytic activity of mitotic cells is restored in mitotic-interphase hybrids.

    abstract::During mitosis there is an abrupt inhibition of a wide range of membrane functions, including fluid-phase and adsorptive pinocytosis. We have used cell hybrids formed between mitotic and interphase cells to approach the mechanism of this inhibition. We report that fluid pinocytosis is reactivated in the mitotic partne...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Oliver JM,Seagrave JC

    更新日期:1987-08-01 00:00:00