Abstract：
:Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation (ter) of the dead-end homolog 1 gene (Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformation and loss of germ cells. We mated heterozygous ter/+ WKY-Dnd1(ter)/Ztm rats and were able to cultivate the first genital ridge-derived EGCs of the rat embryo at day 14.5 post coitum (pc). Genotyping revealed that ten EGC lines were Dnd1 deficient, while only one wild type cell line had survived in culture. This suggests that the inactivation of the putative tumor suppressor gene Dnd1 facilitates the immortalization of late EGCs in vitro. Injection of the wild type EGCs into blastocysts resulted in the first germ-line competent chimeras. These new pluripotent rat EGCs offer an innovative approach for studies on germ cell tumor development as well as a new tool for genetic manipulations in rats.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Northrup E,Eisenblätter R,Glage S,Rudolph C,Dorsch M,Schlegelberger B,Hedrich HJ,Zschemisch NHdoi
10.1016/j.yexcr.2011.04.013subject
Has Abstractpub_date
2011-08-01 00:00:00pages
1885-94issue
13eissn
0014-4827issn
1090-2422pii
S0014-4827(11)00150-9journal_volume
317pub_type
杂志文章abstract：:Various cell adhesion molecules mediate the diverse functions of the vascular endothelium, such as cell adhesion, neutrophil migration, and angiogenesis. In order to identify cell adhesion molecules important for angiogenesis, we used an in vitro model (Chalupowicz, Chowdhury, Bach, Barsigian, and Martinez, J. Cell Bi...
journal_title：Experimental cell research
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abstract：:High glucose concentrations associated with diabetes have been shown to cause the nonenzymatic modification of proteins. Reducing sugars covalently bind to free amine groups, undergo Amadori rearrangements, and crosslink with other glucose-modified proteins. Crosslinking of type I collagen by incubation with different...
journal_title：Experimental cell research
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pub_type： 杂志文章,评审
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2008-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1988-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2018-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2002-06-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.04.012
更新日期：2013-08-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0281
更新日期：1996-09-15 00:00:00
