Interferon alpha induces cell death through interference with interleukin 6 signaling and inhibition of STAT3 activity.


:In multiple myeloma, which commonly depends on interleukin 6, IL-6, survival signaling induced by this cytokine is largely mediated by activation of STAT3. Interferon alpha (IFNalpha) treatment of cell lines derived from multiple myeloma or of myeloma tumor cells ex vivo leads to apoptosis. In this study we demonstrate that IFNalpha treatment of the two myeloma cell lines, U266-1984 and U-1958, results in the decrease of STAT3 activity as demonstrated by a diminished STAT3/3 DNA-binding activity and the shift from STAT3/3 towards STAT1/1 and STAT3/1 complexes in EMSA, leading to the down-regulation of known STAT3 target genes such as Bcl-X(L), Mcl-1 and survivin. Ectopic expression of a form of STAT3, STAT3C, rescued U266-1984 cells from IFNalpha-induced apoptosis. IFNalpha promoted sustained accumulation of tyrosine phosphorylated STAT3C in the nucleus and a prolonged DNA binding of the STAT3/3 homodimers in EMSA. The shift towards a sustained STAT3 response in IFNalpha-treated STAT3C-transfected cells led to a hyper-induction of Bcl-2 and Mcl-1 proteins. Thus our data demonstrated that IFNalpha is able to interfere with IL-6 signaling by inhibiting STAT3 activity and that the abrogation of STAT3 activity accounts for the ability of IFNalpha to induce apoptosis in myeloma cells.


Exp Cell Res


Thyrell L,Arulampalam V,Hjortsberg L,Farnebo M,Grandér D,Pokrovskaja Tamm K




Has Abstract


2007-11-15 00:00:00














  • Thyrotropin-induced formation of functional follicles in primary cultures of ovine thyroid cells.

    abstract::In primary cultures of ovine thyroid cells, TSH induced the expression of several differentiated functions including the formation of follicles, and synthesis and storage of iodinated thyroglobulin in the follicular lumen. In the present report, these follicles were shown by transmission (TEM) and scanning electron mi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mak WW,Errick JE,Chan RC,Eggo MC,Burrow GN

    更新日期:1986-06-01 00:00:00

  • Nuclear RNAs confined to a reticular compartment between chromosome territories.

    abstract::RNA polymerase II transcripts are confined to nuclear compartments. A detailed analysis of the nuclear topology of RNA from individual genes was performed for transcripts from the marker gene coding for chloramphenicol acetyltransferase, expressed at a high level from the HTLV-1 LTR promoter. The construct was transfe...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bridger JM,Kalla C,Wodrich H,Weitz S,King JA,Khazaie K,Kräusslich HG,Lichter P

    更新日期:2005-01-15 00:00:00

  • Altered sensitivity to retinoid-induced apoptosis associated with changes in the subcellular distribution of Bcl-2.

    abstract::In the acute promyelocytic leukemia cell line NB4, Bcl-2 downregulation occurred as a late event of retinoid-induced differentiation. In the maturation-resistant NB4-R1 subclone, retinoids failed to downregulate Bcl-2 even in the situation of apoptosis massively induced by pan-agonists and RXR-selective agonists. We o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bruel A,Karsenty E,Schmid M,McDonnell TJ,Lanotte M

    更新日期:1997-06-15 00:00:00

  • Cell cycle specificity and reversibility of the inhibitory effect of epidermal G1-chalone on DNA synthesis in partially synchronized RTE-2 keratinocytes in vitro.

    abstract::The specific action of a pig skin fraction enriched in epidermal G1-chalone, a tissue-specific inhibitor of epidermal DNA synthesis, was investigated by means of flow cytofluorometry. The results indicate that G1-chalone inhibits progression of partially synchronized rat tongue epithelial cells (line RTE-2) through th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tiegel A,Richter KH,Marks F

    更新日期:1989-06-01 00:00:00

  • Serum effects on the in vitro differentiation of sea urchin micromeres.

    abstract::When micromeres isolated from the 16-cell stage of Strongylocentrotus purpuratus are cultured in sea water containing 3.5% horse serum, they produce spicules at approximately the same time as in normal development. The serum requirement of the micromeres has been investigated by adding serum at varying intervals after...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McCarthy RA,Spiegel M

    更新日期:1983-12-01 00:00:00

  • Telomere reduction in human liver tissues with age and chronic inflammation.

    abstract::Telomere shortening in human liver with aging and chronic inflammation was examined by hybridization protection assay using telomere and Alu probes. The reduction rate of telomere repeats in normal liver (23 samples from patients 17-81 years old) was 120 bp per year, which is in good agreement with the reported reduct...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Aikata H,Takaishi H,Kawakami Y,Takahashi S,Kitamoto M,Nakanishi T,Nakamura Y,Shimamoto F,Kajiyama G,Ide T

    更新日期:2000-05-01 00:00:00

  • Negative cooperativity between alpha 3 beta 1 and alpha 2 beta 1 integrins in human mammary carcinoma MDA MB 231 cells.

    abstract::The alpha 3 beta 1 integrin has been implicated as a receptor for several matrix components, including collagen, fibronectin, and laminins. The function of alpha 3 beta 1 seems to be very versatile involving cell adhesion to or migration on ECM, establishment of cell-cell contacts in aggregates, as well as linkage to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lichtner RB,Howlett AR,Lerch M,Xuan JA,Brink J,Langton-Webster B,Schneider MR

    更新日期:1998-05-01 00:00:00

  • Recent progress in protocadherin research.

    abstract::Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Suzuki ST

    更新日期:2000-11-25 00:00:00

  • Dissecting the sequence specific functions of alternative N-terminal isoforms of mouse bullous pemphigoid antigen 1.

    abstract::Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins that is involved in cross-linking the cytoskeletal elements and attaching them to cell junctions. BPAG1 null mice develop severe degeneration of sensory neurons that was attributed in part due to the absence of a splice variant called BP...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jefferson JJ,Leung CL,Liem RK

    更新日期:2006-09-10 00:00:00

  • Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress.

    abstract::During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bryantsev AL,Chechenova MB,Shelden EA

    更新日期:2007-01-01 00:00:00

  • A composite CMV-IE enhancer/beta-actin promoter is ubiquitously expressed in mouse cutaneous epithelium.

    abstract::In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sawicki JA,Morris RJ,Monks B,Sakai K,Miyazaki J

    更新日期:1998-10-10 00:00:00

  • Flow cytometric analysis of fluorescein-labeled nerve growth factor binding to A875 human melanoma cells.

    abstract::The interaction of fluorescein-labeled nerve growth factor (NGF) with human melanoma cells (A875) has been studied in order to assess better methodology for rigorous NGF binding studies. The NGF was modified at a single carboxyl group with iodoacetamidofluorescein after reaction with carbodiimide and cystamine. The mo...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kasaian MT,Jacobberger JW,Neet KE

    更新日期:1994-01-01 00:00:00

  • Human dermal fibroblasts escape from the long-term phenocopy of senescence induced by psoralen photoactivation.

    abstract::We have previously shown that following psoralen photoactivation (PUVA treatment) human dermal fibroblasts undergo long-term growth arrest as well as morphological and functional changes reminiscent of cellular senescence [ 1 ]. In the absence of molecular data on what constitutes normal senescence, it has been diffic...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ma W,Wlaschek M,Brenneisen P,Schneider LA,Hommel C,Hellweg C,Sauer H,Wartenberg M,Herrmann G,Meewes C,Boukamp P,Scharffetter-Kochanek K

    更新日期:2002-04-01 00:00:00

  • The nucleoside diphosphate kinase activity of DRnm23 is not required for inhibition of differentiation and induction of apoptosis in 32Dcl3 myeloid precursor cells.

    abstract::DRnm23 belongs to a multigene family which includes nm23-H1, the first bona fide metastasis suppressor gene, nm23-H2, nm23-H4, and nm23-H5. Like nm23-H1, nm23-H2, and nm23-H4, DRnm23 possesses nucleoside diphosphate kinase (NDPK) activity. Upon overexpression in myeloid precursor 32Dcl3 cells, DRnm23 inhibits granuloc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Venturelli D,Cesi V,Ransac S,Engelhard A,Perrotti D,Calabretta B

    更新日期:2000-06-15 00:00:00

  • ES cell cycle rates affect gene targeting frequencies.

    abstract::We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Udy GB,Parkes BD,Wells DN

    更新日期:1997-03-15 00:00:00

  • Genomic response to Wnt signalling is highly context-dependent--evidence from DNA microarray and chromatin immunoprecipitation screens of Wnt/TCF targets.

    abstract::Wnt proteins are important regulators of embryonic development, and dysregulated Wnt signalling is involved in the oncogenesis of several human cancers. Our knowledge of the downstream target genes is limited, however. We used a chromatin immunoprecipitation-based assay to isolate and characterize the actual gene segm...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Railo A,Pajunen A,Itäranta P,Naillat F,Vuoristo J,Kilpeläinen P,Vainio S

    更新日期:2009-10-01 00:00:00

  • Downregulation of USP18 inhibits growth and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma cells by suppressing BCL2L1.

    abstract::Ubiquitin-specific peptidase 18 (USP18) is closely related with hepatitis B virus (HBV), which has been involved in tumourigenesis. However, there has been little research into the role of USP18 on the progression of hepatocellular carcinoma (HCC), especially in HBV-related HCC. In present study, we found that USP18 e...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cai J,Liu T,Jiang X,Guo C,Liu A,Xiao X

    更新日期:2017-09-15 00:00:00

  • Polymorphism of simple sequence repeats may quantitatively regulate gene transcription.

    abstract::The degree of polymorphism, i.e., DNA sequence divergence, of short AT-rich tandemly arranged simple sequence repeats at or near promoters and 5'- untranslated regions of mRNA may quantitatively regulate transcription of tissue-specific genes. Less polymorphic repeats allow greater gene expression. Preferential bindin...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Flickinger R

    更新日期:2020-05-15 00:00:00

  • Cell attachment and spreading on extracellular matrix-coated beads.

    abstract::Parietal yolk sac cells M1536 -B3 grown on cytodex 2 beads deposited an extracellular matrix on the surface of the beads. Cell-free matrix-coated beads were isolated by treatment of the cell monolayer with cytochalasin B (CB) at a concentration of 10 micrograms/ml of phosphate-buffered saline (PBS). The matrix when an...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mai S,Chung AE

    更新日期:1984-06-01 00:00:00

  • Corneal cell-matrix interactions: type VI collagen promotes adhesion and spreading of corneal fibroblasts.

    abstract::Type VI collagen is a nonfibrillar collagen present as a network throughout the chick secondary stroma. Immunolocalization of type VI collagen both in the chick corneal stroma and in other systems demonstrates that type VI collagen is present associated with cells and between striated fibrils. We hypothesize that type...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Doane KJ,Yang G,Birk DE

    更新日期:1992-06-01 00:00:00

  • Differential regulation of protooncogenes c-jun and jun D expressions by protein tyrosine kinase, protein kinase C, and cyclic-AMP mitogenic pathways in dog primary thyrocytes: TSH and cyclic-AMP induce proliferation but downregulate C-jun expression.

    abstract::The expressions of the protooncogenes c-jun and jun D have been investigated in dog thyrocytes in a primary culture whose proliferation is stimulated by three distinct intracellular signaling pathways (1) the thyrotropin (TSH) or forskolin-cyclic-AMP-mediated cascade; (2) the protein kinase C pathway activated by diac...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Reuse S,Pirson I,Dumont JE

    更新日期:1991-10-01 00:00:00

  • The reversal of paraquat-induced mitochondria-mediated apoptosis by cycloartenyl ferulate, the important role of Nrf2 pathway.

    abstract::In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hong GL,Liu JM,Zhao GJ,Wang L,Liang G,Wu B,Li MF,Qiu QM,Lu ZQ

    更新日期:2013-11-01 00:00:00

  • The recycling of a secretory granule membrane protein.

    abstract::We have used N-hydroxysuccinimido-d-biotin as a reagent for labeling proteins exposed at the surface of cultured bovine adrenal chromaffin cells during Ba2+-stimulated secretion. A specific secretory granule membrane constituent, dopamine-beta-hydroxylase (DBH), has been investigated using immunoprecipitation followed...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hunter A,Phillips JH

    更新日期:1989-06-01 00:00:00

  • A melanoma cell line sensitive to expression of a fusion protein binding the retinoblastoma protein family pocket domain.

    abstract::An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Khan S,Collins RJ,Dunn IS

    更新日期:1996-02-25 00:00:00

  • Ruta graveolens water extract inhibits cell-cell network formation in human umbilical endothelial cells via MEK-ERK1/2 pathway.

    abstract::Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gentile MT,Russo R,Pastorino O,Cioffi S,Barbieri F,Illingworth EA,Grieco M,Chambery A,Colucci-D'Amato L

    更新日期:2018-03-01 00:00:00

  • Vascular endothelial growth factor driving aberrant keratin expression pattern contributes to the pathogenesis of psoriasis.

    abstract::Psoriasis is a chronic inflammatory skin disease severely affecting patients' physical and psychological well-being. Aberrant keratin expression in psoriasis plays a crucial role in keratinocyte dysfunction and disease development. Little is yet known about the mechanism of this keratin dysregulation. VEGF (Vascular e...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jiang M,Li B,Zhang J,Hu L,Dang E,Wang G

    更新日期:2017-11-15 00:00:00

  • Effects of depletion of intracellular tetrahydrobiopterin in murine erythroleukemia cells.

    abstract::The biosynthesis of 6(R)-5,6,7,8-tetrahydrobiopterin (BH4) in murine erythroleukemia (MEL) cells is almost completely inhibited by 10 mM, 2,4-diamino-6-hydroxypyrimidine (DAHP), which targets GTP cyclohydrolase. The inhibition results in dephosphorylation of the retinoblastoma gene product, prolongation of the G1-phas...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhuo S,Fan S,Kaufman S

    更新日期:1996-01-10 00:00:00

  • Distinct hydrophobic "patches" in the N- and C-tails of beta-catenin contribute to nuclear transport.

    abstract::β-catenin is a key mediator of Wnt signaling and its deregulated nuclear accumulation can drive cancer progression. While the central armadillo (Arm) repeats of β-catenin stimulate nuclear entry, the N- and C-terminal "tail" sequences are thought to regulate turnover and transactivation. We show here that the N- and C...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sharma M,Jamieson C,Lui C,Henderson BR

    更新日期:2016-11-01 00:00:00

  • Assessment of the hepatocytic differentiation ability of human skin-derived ABCB5+ stem cells.

    abstract::The continuously decreasing willingness for liver donation aggravates treatment of end-stage liver diseases requiring organ transplantation as the only curative strategy. Cell therapy approaches using human hepatocytes or stem cell-derived hepatocyte-like cells may be a therapeutic option out of this dilemma. ABCB5-po...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tietze L,Winkler S,Hempel M,Kluth MA,Tappenbeck N,Ganss C,Dooley S,Christ B

    更新日期:2018-08-15 00:00:00

  • In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells.

    abstract::Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nakahara H,Dennis JE,Bruder SP,Haynesworth SE,Lennon DP,Caplan AI

    更新日期:1991-08-01 00:00:00