Abstract：
:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing factor) and p54(nrb), two highly related proteins involved in transcription and RNA processing, as new binding partners of hnRNP M. HnRNP M was found to co-localize with PSF within a subset of nuclear paraspeckles and to largely co-fractionate with PSF and p54(nrb) in biochemical nuclear matrix preparations. In cells transfected with an alternatively spliced preprotachykinin (PPT) minigene expression of hnRNP M promoted exon skipping while expression of PSF favours exon inclusion. The latter effect was reverted specifically by co-expressing the full length hnRNP M or a deletion mutant capable of interaction with PSF and p54(nrb). Together our data provide new insights and some functional implications on the hnRNP M network of interactions.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Marko M,Leichter M,Patrinou-Georgoula M,Guialis Adoi
10.1016/j.yexcr.2009.10.021subject
Has Abstractpub_date
2010-02-01 00:00:00pages
390-400issue
3eissn
0014-4827issn
1090-2422pii
S0014-4827(09)00461-3journal_volume
316pub_type
杂志文章abstract：:Incubation of isolated rat hepatocytes under conditions which support maximal autophagy (amino acid-free medium) caused a marked alteration in the density distribution of lysosomes in continuous metrizamide gradients (mean peak density reduced from 1.14 to 1.09 g/ml). The autophagic sequestration inhibitor 3-methylade...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90141-7
更新日期：1985-04-01 00:00:00
abstract：:Protein kinase B (Akt1) holds a central role for cellular growth, development, and survival, but the cellular pathways of Akt1 that prevent inflammatory demise in the vascular system remain undefined. Employing a constitutively active form of Akt1 (myristoylated Akt1) in endothelial cells (ECs), we demonstrate that Ak...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.01.021
更新日期：2004-06-10 00:00:00
abstract：:A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxicall...
journal_title：Experimental cell research
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doi：10.1016/0014-4827(88)90204-2
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abstract：:Members of the cadherin family of cell adhesion molecules participate in calcium-dependent cell-cell adhesions that are necessary for the cell sorting events that regulate early developmental processes. Although individual cadherin molecules have been shown to participate in tissue histogenesis, the regulation of func...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4819
更新日期：2000-04-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.003
更新日期：2013-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1322
更新日期：1995-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90009-w
更新日期：1992-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.08.011
更新日期：2006-11-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111648
更新日期：2019-11-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1993-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112364
更新日期：2020-12-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.07.014
更新日期：2005-10-15 00:00:00
abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.12.026
更新日期：2008-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.021
更新日期：2005-03-10 00:00:00
abstract：:Quiescence, self-renewal, lineage commitment and differentiation of hematopoietic stem cells (HSCs) towards fully mature blood cells are a complex process that involves both intrinsic and extrinsic signals. During steady-state conditions, most hematopoietic signals are provided by various resident cells inside the bon...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2014.09.016
更新日期：2014-12-10 00:00:00
abstract：:Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3891
更新日期：1998-02-25 00:00:00
abstract：:The duplication and segregation of the genome during cell division is crucial to maintain cell identity, development of organisms and tissue maintenance. Centromeres are at the basis of accurate chromosome segregation as they define the site of assembly of the kinetochore, a large complex of proteins that attaches to ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2020.111909
更新日期：2020-04-15 00:00:00
abstract：:The mitochondrial enzyme cytochrome c oxidase (COX) in eukaryotes consists of at least seven subunits, three of which (I-III) are encoded by mitochondrial DNA (mitDNA) and the others (IV-VII) by the nuclear genome. There is increasing evidence that COX in mammals exists in multiple tissue-specific forms, presumably sp...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90414-9
更新日期：1987-01-01 00:00:00
abstract：:ING1b is the most studied ING family protein and perhaps the most ubiquitously and abundantly expressed. This protein is involved in the regulation of various biological functions ranging from senescence, cell cycle arrest, apoptosis, to DNA repair. ING1b is upregulated by UV irradiation and enhances the removal of bu...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.02.010
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:Breast cancer is the major cause of cancer-related deaths in females world-wide. Doxorubicin-based therapy has limited efficacy in breast cancer due to drug resistance, which has been shown to be associated with the epithelial-to-mesenchymal transition (EMT). However, the molecular mechanisms linking the EMT...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.08.023
更新日期：2013-12-10 00:00:00
abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.10.013
更新日期：2017-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90347-3
更新日期：1988-11-01 00:00:00
abstract：:The mitochondrion imports and processes the vast majority of the proteins that constitute its structural elements and metabolic pathways. To study mitochondrial precursor processing in the context of the cellular environment, we employed the baculovirus expression system to overexpress the prototypical precursor prote...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4331
更新日期：1999-02-01 00:00:00
abstract：:Autophagy has been shown to be critically associated with the central mechanisms underlying Parkinson's disease (PD), while the mechanisms contributing to the imbalance of autophagy remain unclear. Small nucleolar RNA host gene 1 (SNHG1), a well-studied long noncoding RNA, has been reported to be significantly increas...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111614
更新日期：2019-11-01 00:00:00
abstract：:Uptake and metabolism of thymidine and adenosine have been studied in embryos of the sea urchin Strongylocentrotus purpuratus. Uptake of these nucleosides is found to be mutually competitive, with the Km for uptake of thymidine similar to its Ki for inhibition of adenosine uptake and vice versa. The metabolic studies ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90411-3
更新日期：1987-01-01 00:00:00
abstract：:Extracellular matrix (ECM) has been reported to enhance epithelial cell attachment and proliferation as well as to induce differentiation in vitro. In an attempt to determine the benefits of culturing pancreatic islet cells on ECM, we studied the morphological and functional patterns of rat islet cells and an insulin-...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(85)80005-7
更新日期：1985-08-01 00:00:00
abstract：:An adenocarcinoma cell line which has the ability to arrest in G0 phase under exhausted culture conditions was established. Using the cell line, we investigated the expression of cell cycle-associated proteins including cdc2, cdk2, cyclin A, cyclin Dl, and Rb during entry into or withdrawal from the cell cycle. MAP ki...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0159
更新日期：1996-05-25 00:00:00
abstract：:As retinal histogenesis proceeds there is a pronounced increase in the expression of fibroblast growth factor (FGF), reaching its maximum in the mature retina and largely in terminal differentiated retinal neurons. Recent in vivo evidence suggests that exogenous FGF functions as a differentiation and survival factor f...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4048
更新日期：1998-05-25 00:00:00
abstract：:The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5652
更新日期：2002-12-10 00:00:00
abstract：:Cell-CAM 105 is an integral cell surface glycoprotein that is involved in cell-cell adhesion of adult rat hepatocytes in vitro. In the present report we used a radio-immunoassay, a quantitative immunoblotting technique and immunofluorescence microscopy to investigate the expression of cell-CAM 105 in fetal and regener...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90458-1
更新日期：1986-05-01 00:00:00
