Genetics, epigenetics and back again: Lessons learned from neocentromeres.


:The duplication and segregation of the genome during cell division is crucial to maintain cell identity, development of organisms and tissue maintenance. Centromeres are at the basis of accurate chromosome segregation as they define the site of assembly of the kinetochore, a large complex of proteins that attaches to spindle microtubules driving chromosome movement during cell division. Here we summarize nearly 40 years of research focussed on centromere specification and the role of local cis elements in creating a stable centromere. Initial discoveries in budding yeast in the 1980s opened up the field and revealed essential DNA sequence elements that define centromere position and function. Further work in humans discovered a centromeric DNA sequence-specific binding protein and centromeric α-satellite DNA was found to have the capacity to seed centromeres de novo. Despite the early indication of genetic elements as drivers of centromere specification, the discovery in the nineties of neocentromeres that form on unrelated DNA sequences, shifted the focus to epigenetic mechanisms. While specific sequence elements appeared non-essential, the histone H3 variant CENP-A was identified as a crucial component in centromere specification. Neocentromeres, occurring naturally or induced experimentally, have become an insightful tool to understand the mechanisms for centromere specification and will be the focus of this review. They have helped to define the strong epigenetic chromatin-based component underlying centromere inheritance but also provide new opportunities to understand the enigmatic, yet crucial role that DNA sequence elements play in centromere function and inheritance.


Exp Cell Res


Murillo-Pineda M,Jansen LET




Has Abstract


2020-04-15 00:00:00














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    abstract::The macrophages have different subtypes with different functions in immune response and disease. It has been generally accepted that M1 macrophages are responsible for stimulation of immune system and inflammation while M2 macrophages play a role in tissue repair. Irrespective of the type, macrophage functions depend ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liu Y,Minze LJ,Mumma L,Li XC,Ghobrial RM,Kloc M

    更新日期:2016-02-15 00:00:00

  • Specific modulation of surface receptors in J.774 macrophages by anchorage.

    abstract::The J.774 murine macrophage cells were cultured in suspension in Teflon flasks. When allowed to attach on culture plastic dishes, a 2-3-fold increase in transferrin binding was observed. This occurred in 10 min, reached a steady state at 60 min, remained stable for several hours and was reversible after resuspension o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ventura MA,Louache F,Rouis M,Erlich D,Goldstein S,Testa U,Thomopoulos P

    更新日期:1987-06-01 00:00:00

  • Studies on mammalian chromosome replication. III. Organization and replication of diplochromosomes.

    abstract::The process of DNA synthesis in normal and endoreduplicating mammalian cells are very similar. Both types of chromosomes are replicated in defined units termed chromosomal replicons, and in the same sequences along their lengths. The sister chromosomes of the diplochromosomes are replicated synchronously in identical ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lau YF

    更新日期:1983-07-01 00:00:00

  • CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing.

    abstract::We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Maita H,Kitaura H,Ariga H,Iguchi-Ariga SM

    更新日期:2005-02-15 00:00:00

  • Protein kinase C is not involved in the homologous desensitization of bombesin responses in small cell lung cancer cells.

    abstract::Homologous desensitization of responses to bombesin in small cell lung cancer cells can be mimicked by protein kinase C-activating phorbol esters. We show that phorbol ester-induced desensitization can be blocked by prior treatment with the protein kinase C inhibitor staurosporine or prolonged pretreatment with phorbo...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Frankel A,Viallet J

    更新日期:1993-12-01 00:00:00

  • Cellular fibronectin expression is down-regulated at the mRNA level in differentiating human intestinal epithelial cells.

    abstract::The expression of human cellular fibronectin (HFN) was analyzed in relation to the differentiation process of the human enterocyte-like cell line Caco-2. A single approximately 290-kDa form of HFN, corresponding to a 10.0-kb mRNA species, was observed in Caco-2 cells. The expression levels of both protein and mRNA wer...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Vachon PH,Simoneau A,Herring-Gillam FE,Beaulieu JF

    更新日期:1995-01-01 00:00:00

  • Calpain translocation during muscle fiber necrosis and regeneration in dystrophin-deficient mice.

    abstract::Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Spencer MJ,Tidball JG

    更新日期:1996-08-01 00:00:00

  • The acquired radioresistance in HeLa cells under conditions mimicking hypoxia was attenuated by a decreased expression of HIF subunit genes induced by RNA interference.

    abstract::The cancer cells residing in the hypoxic layer are resistant to radiation and these are ones responsible for cancer recurrence after radiation therapy. One of the reasons why hypoxic cancer cells acquire radioresistance may be attributable to changes in the gene expression profile by the activation of hypoxia inducibl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Doi N,Ogawa R,Cui ZG,Morii A,Watanabe A,Kanayama S,Yoneda Y,Kondo T

    更新日期:2015-05-01 00:00:00

  • Macaque trophoblast migration is regulated by RANTES.

    abstract::In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thirkill TL,Lowe K,Vedagiri H,Blankenship TN,Barakat AI,Douglas GC

    更新日期:2005-05-01 00:00:00

  • Cortactin involvement in the keratinocyte growth factor and fibroblast growth factor 10 promotion of migration and cortical actin assembly in human keratinocytes.

    abstract::Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ceccarelli S,Cardinali G,Aspite N,Picardo M,Marchese C,Torrisi MR,Mancini P

    更新日期:2007-05-15 00:00:00

  • Effect of aging on populations of estrogen receptor-containing cells in the rat uterus.

    abstract::Estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. Cell numbers and density were assessed in the various uterine layers. It was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, an...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Han Z,Kokkonen GC,Roth GS

    更新日期:1989-01-01 00:00:00

  • Transforming NRK cells with avian sarcoma virus reduces the extracellular Ca2+ requirement without affecting the calcicalmodulin requirement for the G1/S transition.

    abstract::NRK rat cells infected with a transformation-defective, temperature-sensitive (ts) mutant of the avian sarcoma virus could not proliferate in Ca2+-deficient medium at a nonpermissive temperature (40 degrees C) that inactivated the viral pp60v-src-transforming product and rendered the cells phenotypically untransformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Durkin JP,Whitfield JF

    更新日期:1985-04-01 00:00:00

  • Concomitant alterations of desmosomes, adhesiveness, and diffusion through gap junction channels in a rat ovarian transformation model system.

    abstract::Gap junctional intercellular communication (GJIC), desmosomes, and cell movement were evaluated in a rat ovarian epithelial cell model system which consisted of an immortalized clonal cell line (SIGC), a pSV3neotransfected clonal derivative (SV-SIGC), and a nude mouse SV-SIGC-tumor-derived cell line (T-SV-SIGC). Compl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Stein LS,Stein DW,Echols J,Burghardt RC

    更新日期:1993-07-01 00:00:00

  • Growth stimulation by retinol apparently independent of EGF receptor.

    abstract::A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Böning W,Kakunaga T,Emura M,Mohr U

    更新日期:1986-03-01 00:00:00

  • Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells.

    abstract::We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...

    journal_title:Experimental cell research

    pub_type: 杂志文章,收录出版


    authors: Ozeki N,Mogi M,Hase N,Hiyama T,Yamaguchi H,Kawai R,Matsumoto T,Nakata K

    更新日期:2016-09-10 00:00:00

  • Isolation and characterization of nuclear particles containing rapidly labelled hnRNA and snRNA in combination with a distinct set of polypeptides of Mr 74000 and 72000.

    abstract::A heterogeneous RNP structure has been isolated from rat liver nuclei by a method previously used for the isolation of 30S RNP complexes carrying heterogeneous RNA (hnRNA) [1]. The RNP sediments in sucrose gradients with s-values of 70-110S. Formaldehyde-fixed preparations band at Q = 1.40 in isopycnic CsCl gradients....

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hatzoglou M,Adamtziki E,Margaritis L,Sekeris CE

    更新日期:1985-03-01 00:00:00

  • Hair follicle-specific keratins and their diseases.

    abstract::The human keratin family comprises 54 members, 28 type I and 26 type II. Out of the 28 type I keratins, 17 are epithelial and 11 are hair keratins. Similarly, the 26 type II members comprise 20 epithelial and 6 hair keratins. As, however, 9 out of the 37 epithelial keratins are specifically expressed in the hair folli...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Schweizer J,Langbein L,Rogers MA,Winter H

    更新日期:2007-06-10 00:00:00

  • miR-203a is involved in HBx-induced inflammation by targeting Rap1a.

    abstract::Hepatitis B virus (HBV) causes acute and chronic hepatitis, and is one of the major causes of cirrhosis and hepatocellular carcinoma. Accumulating evidence suggests that inflammation is the key factor for liver cirrhosis and hepatocellular carcinoma. MicroRNAs play important roles in many biological processes. Here, w...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wu A,Chen H,Xu C,Zhou J,Chen S,Shi Y,Xu J,Gan J,Zhang J

    更新日期:2016-11-15 00:00:00

  • U73122 blocked the cGMP-induced calcium release in sea urchin eggs.

    abstract::U73122, a phospholipase C inhibitor, dose dependently blocks the cGMP-induced Ca2+ release in sea urchin eggs and homogenates. U73122 inhibition was prevented by cotreatment with dithiothreitol (DTT), but DTT is ineffective when eggs or homogenates were pretreated with U73122. U73122 action is different from the other...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lee SJ,Madden PJ,Shen SS

    更新日期:1998-07-10 00:00:00

  • Human immunodeficiency virus type 1 Tat protein modulates cell cycle and apoptosis in Epstein-Barr virus-immortalized B cells.

    abstract::Patients infected with human immunodeficiency virus type 1 (HIV-1) develop a spectrum of B cell lymphoproliferative disorders ranging from polyclonal B cell activation to B cell lymphomas. While a direct role of Epstein-Barr virus (EBV) is well recognized for most of these lesions, recent findings have suggested that ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Colombrino E,Rossi E,Ballon G,Terrin L,Indraccolo S,Chieco-Bianchi L,De Rossi A

    更新日期:2004-05-01 00:00:00

  • Isolation of a G0-specific ts mutant from a Fischer rat cell line, 3Y1.

    abstract::A ts mutant clone, tsJT60, was isolated from Fisher rat cell line, 3Y1. During the exponential growth at both 34 and 39.5 degrees C, tsJT60 did not appear as ts mutant cells. However, once entered resting state (G0) under serum deprivation at the confluent state, they could re-enter S phase at 34 degrees C but could n...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ide T,Ninomiya J,Ishibashi S

    更新日期:1984-01-01 00:00:00

  • MT1-MMP initiates activation of pro-MMP-2 and integrin alphavbeta3 promotes maturation of MMP-2 in breast carcinoma cells.

    abstract::We evaluated cellular mechanisms involved in the activation pathway of matrix prometalloproteinase-2 (pro-MMP-2), an enzyme implicated in the malignant progression of many tumor types. Membrane type-1 matrix metalloproteinase (MT1-MMP) cleaves the N-terminal prodomain of pro-MMP-2 thus generating the activation interm...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Deryugina EI,Ratnikov B,Monosov E,Postnova TI,DiScipio R,Smith JW,Strongin AY

    更新日期:2001-02-15 00:00:00

  • PC13 embryonal carcinoma cells produce a heparin-binding growth factor.

    abstract::A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: van Veggel JH,van Oostwaard TM,de Laat SW,van Zoelen EJ

    更新日期:1987-04-01 00:00:00

  • Separation of phenotypically distinct subpopulations of cultured human retinal pigment epithelial cells.

    abstract::Like most epithelial cells that are isolated from tissues and placed in culture, the phenotype of human retinal pigment epithelial cells (RPE) in vitro is more variable than for the same cells in situ. The phenotypic heterogeneity of the cultures has been attributed to varying stages of differentiation of the cells in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McKay BS,Burke JM

    更新日期:1994-07-01 00:00:00

  • CIN85 associates with TNF receptor 1 via Src and modulates TNF-alpha-induced apoptosis.

    abstract::CIN85 is a multidomain protein that associates with receptors carrying tyrosine kinase domains. Here we report that it is also a component of the signaling complex associated with tumor necrosis factor receptor 1 (TNFR1), which lacks a tyrosine kinase domain. This was established by showing that CIN85 was co-precipita...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Narita T,Nishimura T,Yoshizaki K,Taniyama T

    更新日期:2005-03-10 00:00:00

  • Microfilament regulatory protein MENA increases activity of RhoA and promotes metastasis of hepatocellular carcinoma.

    abstract::Mammalian enabled (MENA), usually known as a direct regulator of microfilament polymerization and bundling, promotes metastasis in various cancers. Here we focus on the role of MENA in hepatocellular carcinoma (HCC) metastasis and the relevant mechanism from the view of RhoA activity regulation. By HCC tissue microarr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lin L,Yang XM,Li J,Zhang YL,Qin W,Zhang ZG

    更新日期:2014-09-10 00:00:00

  • Role of esterase gp70 and its influence on growth and development of Dictyostelium discoideum.

    abstract::Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yuan A,Chia CP

    更新日期:2000-12-15 00:00:00

  • Immunofluorescent localization of histone H10 in the nuclei of proliferating and differentiating Friend cells.

    abstract::By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all ove...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Banchev T,Srebreva L,Zlatanova J,Tsanev R

    更新日期:1988-07-01 00:00:00

  • Morphological phenotypes of olfactory ensheathing cells display different migratory responses upon Slit-2.

    abstract::Olfactory ensheathing cells (OECs) are a type of glial cells with morphological plasticity in the olfactory system. Cultured OECs display the process-bearing and flattened shape. Our previous studies have shown that the frontal application of Slit-2 gradient induced the collapse of leading front, and reversed the soma...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wang Y,Huang ZH

    更新日期:2012-09-10 00:00:00

  • Alterations in cell tetrahydrobiopterin levels may regulate queuine hypomodification of tRNA during differentiation of murine erythroleukemia cells.

    abstract::The base at the first anticodon ("wobble") position of certain eukaryotic tRNA species is either guanine or the hypermodified base queuine. These tRNA species are synthesized with guanine in the wobble position (tRNAG); this guanine can then be replaced with queuine by the action of the enzyme tRNA-guanine ribosyltran...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Parniak MA,Andrejchyshyn S,Marx S,Kleiman L

    更新日期:1991-07-01 00:00:00