N-cadherin function is required for differentiation-dependent cytoskeletal reorganization in lens cells in vitro.


:Members of the cadherin family of cell adhesion molecules participate in calcium-dependent cell-cell adhesions that are necessary for the cell sorting events that regulate early developmental processes. Although individual cadherin molecules have been shown to participate in tissue histogenesis, the regulation of function of these receptors in cell differentiation has been more difficult to identify. We have determined that N-cadherin linkage to the cytoskeleton is correlated with lens cell differentiation in vivo. Through the use of a chick embryo lens culture system that mimics differentiation in vivo, we have determined that N-cadherin linkage to the cytoskeleton is altered and lens differentiation is blocked by function-blocking antibodies to N-cadherin. In the presence of the N-cadherin function-blocking antibody, NCD-2, both N-cadherin and filamentous actin are prevented from organizing at the cortical membranes. This correlates with an inhibition of lens morphogenesis and differentiation. These results are paralleled by changes in the expression of the molecular components of the cadherin-catenin complex and their linkage to the actin cytoskeleton. In the presence of NCD-2, expression of N-cadherin, alpha-catenin, and beta-catenin is inhibited and their association with the cytoskeleton blocked. Overall cadherin expression, however, remains unchanged as demonstrated by studies with a pan-cadherin antibody. This is accompanied by an increase in expression of the cadherin cytoskeletal protein plakoglobin. Although the cells have tried to compensate for the loss of N-cadherin by up-regulation of another cadherin(s) and plakoglobin, this is unable to compensate for N-cadherin function. The data strongly suggest that N-cadherin and its associated cytoskeleton play an important role in the differentiation process that leads to the formation of the crystalline lens.


Exp Cell Res


Ferreira-Cornwell MC,Veneziale RW,Grunwald GB,Menko AS





Has Abstract


2000-04-10 00:00:00














  • Characteristics of 25-hydroxycholesterol-induced apoptosis in the human leukemic cell line CEM.

    abstract::Cholesterol and related compounds can give rise to oxygenated sterol molecules (oxysterols) which are potent regulators of lymphoid cell growth. Oxysterols added exogenously cause cell death of several lines of cultured cells, and on the basis of limited criteria, it has been suggested that this death is apoptosis. In...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ayala-Torres S,Moller PC,Johnson BH,Thompson EB

    更新日期:1997-08-25 00:00:00

  • The activated insulin-like growth factor I receptor induces depolarization in breast epithelial cells characterized by actin filament disassembly and tyrosine dephosphorylation of FAK, Cas, and paxillin.

    abstract::Insulin-like growth factor I (IGF-I) promotes the motility of different cell types. We investigated the role of IGF-I receptor (IGF-IR) signaling in locomotion of MCF-7 breast cancer epithelial cells overexpressing the wild-type IGF-IR (MCF-7/IGF-IR). Stimulation of MCF-7/IGF-IR cells with 50 ng/ml IGF-I induced disru...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Guvakova MA,Surmacz E

    更新日期:1999-08-25 00:00:00

  • Ontogeny of basic fibroblast growth factor binding sites in mouse ocular tissues.

    abstract::Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fayein NA,Courtois Y,Jeanny JC

    更新日期:1990-05-01 00:00:00

  • A mutant allele of BARA/LIN-9 rescues the cdk4-/- phenotype by releasing the repression on E2F-regulated genes.

    abstract::It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sandoval R,Xue J,Tian X,Barrett K,Pilkinton M,Ucker DS,Raychaudhuri P,Kineman RD,Luque RM,Baida G,Zou X,Valli VE,Cook JL,Kiyokawa H,Colamonici OR

    更新日期:2006-08-01 00:00:00

  • Furin inhibition prevents hypoxic and TGFβ-mediated blood-brain barrier disruption.

    abstract::Hypoxic blood-brain barrier (BBB) dysfunction is a common feature of CNS diseases however mechanisms underlying barrier disturbance are still largely unknown. This study investigated the role of transforming growth factor β (TGFβ), a cytokine known to induce expression of the proprotein convertase Furin, in hypoxia-me...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Baumann J,Huang SF,Gassmann M,Tsao CC,Ogunshola OO

    更新日期:2019-10-15 00:00:00

  • Activation of cysteine proteases in cowpea plants during the hypersensitive response--a form of programmed cell death.

    abstract::There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: D'Silva I,Poirier GG,Heath MC

    更新日期:1998-12-15 00:00:00

  • Simultaneous cell death and desquamation of the embryonic diffusion barrier during epidermal development.

    abstract::The periderm is an epithelial layer covering the emerging epidermis in early embryogenesis of vertebrates. In the chicken embryo, an additional cellular layer, the subperiderm, occurs at later embryonic stages underneath the periderm. The questions arose what is the function of both epithelial layers and, as they are ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Saathoff M,Blum B,Quast T,Kirfel G,Herzog V

    更新日期:2004-10-01 00:00:00

  • Nerve Growth Factor modulates LPS - induced microglial glycolysis and inflammatory responses.

    abstract::Microglia, the parenchymal immune cells of the central nervous system, orchestrate neuroinflammation in response to infection or damage, and promote tissue repair. However, aberrant microglial responses are integral to neurodegenerative diseases and critically contribute to disease progression. Thus, it is important t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fodelianaki G,Lansing F,Bhattarai P,Troullinaki M,Zeballos MA,Charalampopoulos I,Gravanis A,Mirtschink P,Chavakis T,Alexaki VI

    更新日期:2019-04-15 00:00:00

  • Individualizing breast cancer treatment-The dawn of personalized medicine.

    abstract::Identification of breast cancer not being a single disease but backed by multiple heterogeneous oncogenic subpopulations is of growing interest in developing personalized therapies to provide optimal outcomes. Through this review, we bring attention to evolution of tumor and microenvironment heterogeneity as a predomi...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Nandy A,Gangopadhyay S,Mukhopadhyay A

    更新日期:2014-01-01 00:00:00

  • Protein kinase C activity and phorbol ester binding to rat myogenic cells during growth and differentiation.

    abstract::Phorbol esters have been reported to induce opposite responses in fetal myoblasts and in satellite cells isolated from adult skeletal muscles. We examined the possibility that different levels of protein kinase C (PKC) activity and different phorbol ester binding characteristics account for these responses. For this p...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Martelly I,Gautron J,Moraczewski J

    更新日期:1989-07-01 00:00:00

  • Reactivation of an inactive human X chromosome introduced into mouse embryonal carcinoma cells by microcell fusion with persistent expression of XIST.

    abstract::An inactive human X chromosome was introduced by microcell fusion into two mouse embryonal carcinoma cell lines, PSA1-TG8 and OTF9-63, each of which has a single X chromosome. The donor cell line was a mouse-human somatic cell hybrid, CF150, retaining one or more inactive human X chromosome(s) per cell as its only hum...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yoshida I,Nishita Y,Mohandas TK,Takagi N

    更新日期:1997-02-01 00:00:00

  • RalGPS2 is involved in tunneling nanotubes formation in 5637 bladder cancer cells.

    abstract::RalGPS2 is a Ras-independent Guanine Nucleotide Exchange Factor (GEF) for RalA containing a PH domain and an SH3-binding region and it is involved in several cellular processes, such as cytokinesis, control of cell cycle progression, differentiation, cytoskeleton organization and rearrangement. Up to now, few data hav...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: D'Aloia A,Berruti G,Costa B,Schiller C,Ambrosini R,Pastori V,Martegani E,Ceriani M

    更新日期:2018-01-15 00:00:00

  • Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation: TNF-alpha inhibits commitment.

    abstract::We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Castro-Muñozledo F,Beltrán-Langarica A,Kuri-Harcuch W

    更新日期:2003-04-01 00:00:00

  • Cytostatic factor (CSF) activity in cytosols extracted from Xenopus laevis eggs.

    abstract::Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Moses RM,Masui Y

    更新日期:1990-01-01 00:00:00

  • Radiation response and regulation of apoptosis induced by a combination of TRAIL and CHX in cells lacking mitochondrial DNA: a role for NF-κB-STAT3-directed gene expression.

    abstract::Mitochondrial DNA depleted (ρ(0)) human skin fibroblasts (HSF) with suppressed oxidative phosphorylation were characterized by significant changes in the expression of 2100 nuclear genes, encoding numerous protein classes, in NF-κB and STAT3 signaling pathways, and by decreased activity of mitochondrial death pathway,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ivanov VN,Ghandhi SA,Zhou H,Huang SX,Chai Y,Amundson SA,Hei TK

    更新日期:2011-07-01 00:00:00

  • HABP1/p32/gC1qR induces aberrant growth and morphology in Schizosaccharomyces pombe through its N-terminal alpha helix.

    abstract::Hyaluronan binding protein (HABP1), located on human chromosome 17p13.3, was identified and characterized as being involved in cellular signaling from our laboratory. Here, we demonstrate that HABP1 expression in Schizosaccharomyces pombe induces growth inhibition, morphological abnormalities like elongation, multinuc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mallick J,Datta K

    更新日期:2005-10-01 00:00:00

  • Conjugation in the ciliate Euplotes octocarinatus: comparison of ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells.

    abstract::Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of g...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Plümper E,Freiburg M,Heckmann K

    更新日期:1995-04-01 00:00:00

  • The actin cytoskeleton-associated protein zyxin acts as a tumor suppressor in Ewing tumor cells.

    abstract::Changes in cell architecture, essentially linked to profound cytoskeleton rearrangements, are common features accompanying cell transformation. Supporting the involvement of the microfilament network in tumor cell behavior, several actin-binding proteins, including zyxin, a potential regulator of actin polymerization,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Amsellem V,Kryszke MH,Hervy M,Subra F,Athman R,Leh H,Brachet-Ducos C,Auclair C

    更新日期:2005-04-01 00:00:00

  • miR-133b suppresses colorectal cancer cell stemness and chemoresistance by targeting methyltransferase DOT1L.

    abstract::Cancer stem cells (CSCs) are a subpopulation of chemoresistant cells that play a critical role in disease recurrence following chemotherapy. It has been reported that microRNA-133b (miR-133b) acts as a tumor suppressor in colorectal cancer (CRC). However, whether miR-133b is associated with CRC stemness and chemoresis...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lv L,Li Q,Chen S,Zhang X,Tao X,Tang X,Wang S,Che G,Yu Y,He L

    更新日期:2019-12-01 00:00:00

  • Calcium functions as a transcriptional and mitogenic repressor in Syrian hamster embryo cells: roles of intracellular pH and calcium in controlling embryonic cell differentiation and proliferation.

    abstract::We have investigated the role played by the growth and differentiation factor (GDF)-induced calcium ion second messenger signal in the control of cellular differentiation and proliferation in Syrian hamster embryo (SHE) cells. Blocking the platelet-derived growth factor (PDGF)-induced calcium ion signal with either ex...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Isfort RJ,Cody DB,Stuard SB,Ridder GM,LeBoeuf RA

    更新日期:1996-08-01 00:00:00

  • Isolation and characterization of novel peroxisome biogenesis-defective Chinese hamster ovary cell mutants using green fluorescent protein.

    abstract::We developed an improved method for isolation of peroxisome biogenesis-defective somatic animal cell mutants, using a combination of green fluorescent protein (GFP) expression and the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) selection method. We used TKaG1 and TKaG2 cells, the wild-type Chinese hamster ovary (CHO) c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ghaedi K,Kawai A,Okumoto K,Tamura S,Shimozawa N,Suzuki Y,Kondo N,Fujiki Y

    更新日期:1999-05-01 00:00:00

  • Characterization of type II and type XI collagen synthesis by an immortalized rat chondrocyte cell line (IRC) having a low level of type II collagen mRNA expression.

    abstract::The biosynthesis of type XI and type II collagens was examined using a stable rat chondrocyte cell line established by W. E. Horton et al. (1988, Exp. Cell Res. 178, 457-468.). These cells (IRC; immortalized rat chondrocytes) were created by transformation with a murine retrovirus carrying the v-myc and v-raf oncogene...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Oxford JT,Doege KJ,Horton WE Jr,Morris NP

    更新日期:1994-07-01 00:00:00

  • Mitochondrial-dependent regulation of myoblast proliferation.

    abstract::The aim of the present study was to determine whether mitochondrial activity could regulate myoblast proliferation. We demonstrate that an increase in mitochondrial activity of L6E9 myoblasts can be easily obtained by simply raising extracellular pyruvate concentration in the culture dish. Under this condition, L6E9 m...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Duguez S,Sabido O,Freyssenet D

    更新日期:2004-09-10 00:00:00

  • Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+.

    abstract::Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

    更新日期:1998-07-10 00:00:00

  • Cholesterol oxides mediated changes in cytoskeletal organisation involves Rho GTPases.

    abstract::The small GTPases Rho, Rac, and Cdc42 regulate the actin cytoskeleton in all eukaryotic cells. In this study we have evaluated the effect of cholesterol oxides (7-ketocholesterol and 25-hydroxycholesterol) on cell migration, cell adhesion, and cytoskeletal organisation of lens epithelial cells (LEC). Effects of choles...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Girão H,Pereira P,Ramalho J,Quinlan R,Prescott A

    更新日期:2003-12-10 00:00:00

  • Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.

    abstract::Since the migration of human fetal skin fibroblasts into a denuded area of a cell monolayer has been demonstrated to be serum-independent (fetal phenotype), whereas that of adult-donor skin fibroblasts is serum-dependent (adult phenotype), a study was carried out to determine at which life stage the fetal-adult phenot...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kondo H,Yonezawa Y

    更新日期:1995-10-01 00:00:00

  • Changes in elastin-binding proteins during the phenotypic transition of rabbit arterial smooth muscle cells in primary culture.

    abstract::Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yamamoto K,Aoyagi M,Yamamoto M

    更新日期:1995-05-01 00:00:00

  • Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

    abstract::Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-l...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Patel JJ,Bourne LE,Davies BK,Arnett TR,MacRae VE,Wheeler-Jones CP,Orriss IR

    更新日期:2019-07-01 00:00:00

  • Hierarchy of ADAM12 binding to integrins in tumor cells.

    abstract::ADAMs (a disintegrin and metalloprotease) comprise a family of cell surface proteins with protease and cell-binding activities. Using different forms and fragments of ADAM12 as substrates in cell adhesion and spreading assays, we demonstrated that alpha9beta1 integrin is the main receptor for ADAM12. However, when alp...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thodeti CK,Fröhlich C,Nielsen CK,Holck P,Sundberg C,Kveiborg M,Mahalingam Y,Albrechtsen R,Couchman JR,Wewer UM

    更新日期:2005-10-01 00:00:00

  • The zeta isozyme of protein kinase C binds to tubulin through the pseudosubstrate domain.

    abstract::It has been suggested that the protein kinase C zeta (zeta PKC) isoform is involved in mitogenic signaling in Xenopus oocytes and mammalian cells. Thus, the characterization of potential regulatory molecules that bind to zeta PKC is of great interest. We report here the identification by affinity chromatography of tub...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: García-Rocha M,Avila J,Lozano J

    更新日期:1997-01-10 00:00:00