Mitotic segregation of the nucleolar ribosomal RNA in Physarum polycephalum.

abstract：:Skin extracellular matrix (ECM) molecules regulate a variety of cellular activities, including cell movement, which are central to wound healing and metastasis. Regulated cell movement is modulated by proteases and their associated molecules, including the serine proteases urinary-type plasminogen activator (uPA) and ...

journal_title：Experimental cell research

authors： Jones JM,Cohen RL,Chambers DA

更新日期：2002-11-01 00:00:00

abstract：:Human diploid fibroblasts (HDF) rarely, if ever, undergo spontaneous transformation to an immortalized cell type. Here we report the immortalization of an HDF cell line following transduction with cyclin A2 or cdk1 human genes via retroviral vectors. Fluorescence in situ hybridization (FISH) studies using the retrovir...

journal_title：Experimental cell research

authors： Luo P,Tresini M,Cristofalo V,Chen X,Saulewicz A,Gray MD,Banker DE,Klingelhutz AL,Ohtsubo M,Takihara Y,Norwood TH

更新日期：2004-04-01 00:00:00

abstract：:The recruitment of osteoblast progenitors involves their migration and attachment to the sites of bone formation through interactions with matrix proteins. In a time-limited cell attachment assay, coated laminin-1 inhibits the adhesion of most rat calvaria cells but attaches specifically to osteoprogenitors, as quanti...

journal_title：Experimental cell research

authors： Roche P,Rousselle P,Lissitzky JC,Delmas PD,Malaval L

更新日期：1999-08-01 00:00:00

abstract：:The extraocular muscles (EOM) are spared from pathology in aging and many forms of muscular dystrophy. Despite many studies, this sparing remains an enigma. The EOM have a distinct embryonic lineage compared to somite-derived muscles, and we have shown that they continuously remodel throughout life, maintaining a popu...

journal_title：Experimental cell research

authors： Kallestad KM,Hebert SL,McDonald AA,Daniel ML,Cu SR,McLoon LK

更新日期：2011-04-01 00:00:00

abstract：:Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...

journal_title：Experimental cell research

authors： Moses RM,Masui Y

更新日期：1990-01-01 00:00:00

abstract：:1,25-dihydroxyvitamin D(3) (VD(3)) induces differentiation in a number of leukemia cell lines and under various conditions is able to either stimulate or inhibit nuclear factor kappa B (NF-kappaB) activity. Here we report a time-dependent biphasic regulation of NF-kappaB in VD(3)-treated HL-60 leukemia cells. After VD...

journal_title：Experimental cell research

authors： Tse AK,Wan CK,Shen XL,Zhu GY,Cheung HY,Yang M,Fong WF

更新日期：2007-05-01 00:00:00

abstract：:Liver cells isolated from the adult rat livers under mild conditions were preincubated for 1 day with Williams medium E (WE) containing serum, dexamethasone and insulin, and then the cells (monolayered) were incubated for 2-3 days with WE (1 ml) containing only insulin to measure DNA synthesis and/or mitosis. DNA synt...

journal_title：Experimental cell research

authors： Koji T,Terayama H

更新日期：1984-12-01 00:00:00

abstract：:In order to investigate the molecular mechanism of biological action of the drug cyclosporin A (CsA), we have studied its effect on eukaryotic RNA polymerase activities in two systems: (a) in vitro transcription; (b) injection into frog oocytes. The electron microscopic transcription R-loop method [1] has been used to...

journal_title：Experimental cell research

authors： Brack C,Mattaj IW,Gautschi J,Cammisuli S

更新日期：1984-04-01 00:00:00

abstract：:Tob, a member of the Tob and BTG antiproliferative protein family, plays an important role in many cellular processes including cell proliferation. In this study, we have addressed molecular mechanisms regulating subcellular localization of Tob. Treatment with leptomycin B, an inhibitor of nuclear export signal (NES) ...

journal_title：Experimental cell research

authors： Maekawa M,Yamamoto T,Nishida E

更新日期：2004-04-15 00:00:00

abstract：:LMX1B is a LIM-homeodomain transcription factor essential for development. Putative LMX1B target genes have been identified through mouse gene targeting studies, but their identity as direct LMX1B targets remains hypothetical. We describe here the first molecular characterization of LMX1B target gene regulation. Micro...

journal_title：Experimental cell research

authors： Rascle A,Neumann T,Raschta AS,Neumann A,Heining E,Kastner J,Witzgall R

更新日期：2009-01-01 00:00:00

abstract：:During pathological bone loss, factors that are both stimulatory and inhibitory for osteoclast differentiation are over-expressed. Despite the presence of inhibitory factors, osteoclast differentiation is significantly enhanced to bring about bone loss. To examine the hypothesis that stimulatory growth factors overcom...

journal_title：Experimental cell research

authors： Gorny G,Shaw A,Oursler MJ

更新日期：2004-03-10 00:00:00

abstract：:Nuclear and territorial positioning of p- and q-telomeres and centromeres of chromosomes 3, 8, 9, 13, and 19 were studied by repeated fluorescence in situ hybridization, high-resolution cytometry, and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the ...

journal_title：Experimental cell research

authors： Amrichová J,Lukásová E,Kozubek S,Kozubek M

更新日期：2003-09-10 00:00:00

abstract：:The relationship between topoisomerase II activity and ribosomal RNA synthesis was investigated using the antitumoral drug VM26, a specific inhibitor of topoisomerase II. For this purpose TG cells, a human tumor cell line, were cultured in the presence of 2.5 microM VM26 for 1 and 3 h; VM26 reduced the topoisomerase I...

journal_title：Experimental cell research

authors： Govoni M,Farabegoli F,Pession A,Novello F

更新日期：1994-03-01 00:00:00

abstract：:Tumor-derived exosomes (TEX) play an important role in the escape of tumor cells from immune surveillance. However, the details of the mechanism are not fully understood. In this study, the apoptosis of CD4+ T cells increased during treatment with B16-derived exosomes in vitro and in vivo, resulting in accelerated gro...

journal_title：Experimental cell research

authors： Zhou J,Yang Y,Wang W,Zhang Y,Chen Z,Hao C,Zhang J

更新日期：2018-10-15 00:00:00

abstract：:Resistance of cancer cells to apoptosis is dependent on a balance of multiple genetic and epigenetic mechanisms, which up-regulate efficacy of the surviving growth factor-receptor signaling pathways and suppress death-receptor signaling pathways. The Insulin-like Growth Factor-1 Receptor (IGF-1R) signaling pathway is ...

journal_title：Experimental cell research

authors： Karasic TB,Hei TK,Ivanov VN

更新日期：2010-07-15 00:00:00

abstract：:KRIT1 is a disease gene responsible for Cerebral Cavernous Malformations (CCM). It encodes for a protein containing distinct protein-protein interaction domains, including three NPXY/F motifs and a FERM domain. Previously, we isolated KRIT1B, an isoform characterized by the alternative splicing of the 15th coding exon...

journal_title：Experimental cell research

authors： Francalanci F,Avolio M,De Luca E,Longo D,Menchise V,Guazzi P,Sgrò F,Marino M,Goitre L,Balzac F,Trabalzini L,Retta SF

更新日期：2009-01-15 00:00:00

abstract：:To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three ke...

journal_title：Experimental cell research

authors： Walmod PS,Hartmann-Petersen R,Prag S,Lepekhin EL,Röpke C,Berezin V,Bock E

更新日期：2004-05-01 00:00:00

abstract：:There are numerous mechanisms by which mammals respond to hypoxia. These include acute changes in pulmonary arterial tone due to smooth muscle cell contraction, acute increases in respiration triggered by the carotid body chemosensory cells, and chronic changes such as induction of red blood cell proliferation and ang...

journal_title：Experimental cell research

authors： McElroy GS,Chandel NS

更新日期：2017-07-15 00:00:00

abstract：:In order to explore the potential value of Chinese hamster ovary (CHO) cells for the isolation of peroxisomal mutants defective in the peroxisomal fatty acid oxidation system, some characteristics of their peroxisomes were studied. Catalase was detected biochemically and histochemically in peroxisome-like particles in...

journal_title：Experimental cell research

authors： Santos MJ,Garrido J,Oliver C,Robbins AR,Leighton F

更新日期：1985-11-01 00:00:00

abstract：:Hepatocyte growth factor (HGF) is a pleiotropic cytokine promoting proliferation, migration and survival in several cell types. HGF and its cognate receptor c-Met are expressed in cardiac cells during early cardiogenesis, but data concerning its role in cardiac differentiation of embryonic stem cells (ESCs) and the un...

journal_title：Experimental cell research

authors： Roggia C,Ukena C,Böhm M,Kilter H

更新日期：2007-03-10 00:00:00

abstract：:The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...

journal_title：Experimental cell research

authors： Marshall H,Martens I,Svensson C,Akusjärvi G,Linder S

更新日期：1991-05-01 00:00:00

abstract：:Up-regulation of intercellular adhesion molecule-1 (ICAM-1) and its soluble form are involved in the chronic inflammation. For the first time, we demonstrated that resveratrol (RE), a natural polyphenol with antioxidant and anti-inflammatory properties, reduces the increase of expression and release of ICAM-1, due to ...

journal_title：Experimental cell research

authors： Domazetovic V,Bonanomi AG,Stio M,Vincenzini MT,Iantomasi T

更新日期：2019-09-15 00:00:00

abstract：:Experiments are described which show that the torsional oscillations arising in a strand of the slime mould, Physarum polycephalum, under certain conditions remain closely correlated to oscillations in cytoplasmic streaming. When this is the case, the direction of rotation is related to the direction of streaming by a...

journal_title：Experimental cell research

authors： Obendorf P,O'Sullivan RA

更新日期：1983-11-01 00:00:00

abstract：:Previous morphological and biochemical studies have suggested that actin and actin-containing filaments (microfilaments) exist in the eukaryotic nucleus and that they perform important nuclear functions. However, the concept is not widely accepted. In this study, we demonstrate actin and bundles of actin in the nuclei...

journal_title：Experimental cell research

authors： Parfenov VN,Davis DS,Pochukalina GN,Sample CE,Bugaeva EA,Murti KG

更新日期：1995-04-01 00:00:00

abstract：:In preparation for studies on the growth factor requirements of normal and transformed human fibroblasts, we have developed a serum-free medium that supports vigorous long-term serial subculture of diploid human fibroblasts and allows them to form large-sized colonies with high efficiency (40 to 60%) when plated at cl...

journal_title：Experimental cell research

authors： Ryan PA,Maher VM,McCormick JJ

更新日期：1987-10-01 00:00:00

abstract：BACKGROUND:The db/db mouse is an animal model of diabetes in which leptin receptor activity is deficient resulting accelerated cardiomyopathy when exposed to angiotensin (AT). Toll-like receptors 4 and 2 (TLR4, TLR2) are pattern recognition receptors, that recognize pathogen-associated molecular patterns and exacerbate...

journal_title：Experimental cell research

authors： Cohen K,Waldman M,Abraham NG,Laniado-Schwartzman M,Gurfield D,Aravot D,Arad M,Hochhauser E

更新日期：2017-01-01 00:00:00

abstract：:We have investigated whether restriction endonucleases produce bands on human chromosomes by extracting DNA, using staining methods which are stoichiometric for DNA. Restriction enzymes that produce C-band patterns appear to remove DNA extensively from chromosome arms. In general, however, those restriction enzymes th...

journal_title：Experimental cell research

authors： Mezzanotte R,Ferrucci L,Vanni R,Sumner AT

更新日期：1985-11-01 00:00:00

abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...

journal_title：Experimental cell research

authors： Stuurman N,Van Driel R,De Jong L,Meijne AM,Van Renswoude J

更新日期：1989-02-01 00:00:00

abstract：:Transepithelial resistance (TER) measurement has often been used to study the paracellular transport properties of epithelia grown on permeable filters, especially the barrier function of tight junctions. However, the TER value includes another source, the resistance caused by cell-substrate contact, that may give ris...

journal_title：Experimental cell research

authors： Lo CM,Keese CR,Giaever I

更新日期：1999-08-01 00:00:00

abstract：:Hereditary transthyretin amyloidosis (ATTR) is caused by amyloid deposition of misfolded transthyretin (TTR) in various tissues. Recently, reduction of circulating serum TTR, achieved via silencing oligonucleotides, was introduced as therapy of ATTR amyloidosis. We explored the impact of Serpin Family A Member 1 (SERP...

journal_title：Experimental cell research

authors： Niemietz C,Bezerra F,Almeida MR,Guo S,Monia BP,Saraiva MJ,Schütz P,Schmidt HH,Zibert A

更新日期：2020-10-15 00:00:00