当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Developmental changes in RNA polymerase I and TATA box-binding protein during early Xenopus embryogenesis.

Developmental changes in RNA polymerase I and TATA box-binding protein during early Xenopus embryogenesis.

Abstract：

:Xenopus early embryos are transcriptionally quiescent until the midblastula transition (MBT). We have examined the question of whether the absence of rRNA synthesis is related to a deficiency in the RNA polymerase I (pol I) transcription machinery. Previously we have demonstrated that the maternally provided pol I transcription factor UBF already binds to the inactive rRNA genes of pre-MBT embryos (P. Bell et al., 1997, J. Cell Sci. 110, 2053-2063). Here we have analyzed the fate of pol I and the TATA box-binding protein (TBP) through immunofluorescence and immunoblotting experiments. Pol I stockpiled in the egg is taken up by in vitro assembled pronuclei and concentrated into numerous distinct nuclear domains. Comparable storage sites of template-free pol I are also seen in nuclei of blastula to neurula stage embryos. In contrast, the amount of TBP is relatively low in oocytes and eggs but increases dramatically during the cleavage stages. Most of the newly synthesized TBP colocalizes with the stored form of pol I in the extranucleolar domains of blastula/gastrula embryos. The amount of TBP per embryo reaches peak values at the blastula/gastrula stage and then rapidly declines to normal somatic levels. The positive correlation of maximal TBP levels with the timing of the MBT suggests that overproduction of TBP is required for the formation of productive transcription complexes.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Bell P,Scheer U

doi

10.1006/excr.1999.4411

keywords:

subject

Has Abstract

pub_date

1999-04-10 00:00:00

pages

122-35

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(99)94411-7

journal_volume

248

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Calpains: markers of tumor aggressiveness?

    abstract：:Rhabdomyosarcoma (RMS) are soft-tissue sarcoma commonly encountered in childhood. RMS cells can acquire invasive behavior and form metastases. The metastatic dissemination implicates many proteases among which are mu-calpain and m-calpain. Study of calpain expression and activity underline the deregulation of calpain ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.017

    authors： Roumes H,Leloup L,Dargelos E,Brustis JJ,Daury L,Cottin P

    更新日期：2010-05-15 00:00:00

  • Induction of ANGPTL4 expression in human airway smooth muscle cells by PMA through activation of PKC and MAPK pathways.

    abstract：:In this study, we demonstrate that protein kinase C (PKC) activators, including phorbol-12-myristate-13-acetate (PMA), 1,2-dioctanoyl-sn-glycerol (DOG), and platelet-derived growth factor alpha are potent inducers of angiopoietin-like protein 4 (ANGPTL4) expression in several normal lung cell types and carcinoma cell ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.12.004

    authors： Stapleton CM,Joo JH,Kim YS,Liao G,Panettieri RA Jr,Jetten AM

    更新日期：2010-02-15 00:00:00

  • Cytofluorometric DNA base determination for the investigation of heterochromatin and heterochromatin amplification.

    abstract：:The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90223-9

    authors： Leemann U,Ruch F

    更新日期：1983-09-01 00:00:00

  • Intracellular distribution of poly(ADP-ribose) synthetase in rat spermatogenic cells.

    abstract：:The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemic...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90063-3

    authors： Concha II,Figueroa J,Concha MI,Ueda K,Burzio LO

    更新日期：1989-02-01 00:00:00

  • The RING finger protein RNF11 is expressed in bone cells during osteogenesis and is regulated by Ets1.

    abstract：:Maturation of MC3T3-E1 osteoblast cells in vitro can be divided into three major stages, namely, proliferation, differentiation, and mineralization. Our microarray analysis identified genes differentially expressed between proliferating and differentiated MC3T3-E1 osteoblastic cells. Immunohistochemical analyses of RN...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.031

    authors： Gao Y,Ganss BW,Wang H,Kitching RE,Seth A

    更新日期：2005-03-10 00:00:00

  • Long-term cultivation of amphibian melanophores. In vitro ageing and spontaneous transformation to a continuous cell line.

    abstract：:Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in gr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90396-8

    authors： Kondo H,Ide H

    更新日期：1983-11-01 00:00:00

  • Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells.

    abstract：:Inorganic polyphosphate [Poly(P)] may represent a physiological source of phosphate and has the ability to induce bone differentiation in osteoblasts. We previously reported that cytokine-induced matrix metalloproteinase (MMP)-3 accelerates the proliferation of purified odontoblast-like cells. In this study, MMP-3 sma...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.01.007

    authors： Ozeki N,Hase N,Yamaguchi H,Hiyama T,Kawai R,Kondo A,Nakata K,Mogi M

    更新日期：2015-05-01 00:00:00

  • Mitotic segregation of the nucleolar ribosomal RNA in Physarum polycephalum.

    abstract：:In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1274

    authors： Pierron G,Puvion-Dutilleul F

    更新日期：1993-10-01 00:00:00

  • Establishment of a germ-line competent C57BL/6 embryonic stem cell line.

    abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90430-3

    authors： Ledermann B,Bürki K

    更新日期：1991-12-01 00:00:00

  • Induction of EGF-dependent apoptosis by vacuolar-type H(+)-ATPase inhibitors in A431 cells overexpressing the EGF receptor.

    abstract：:The stimulation of human tumor cells overexpressing epidermal growth factor receptor (EGFR) with EGF enhances tumor development and malignancy. Therefore, compounds that modulate the EGF-mediated signal inducing apoptosis in EGFR-overexpressing cells would represent a new class of antitumor drug and might be useful in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5588

    authors： Yoshimoto Y,Imoto M

    更新日期：2002-09-10 00:00:00

  • Fibroblast contractile force is independent of the stiffness which resists the contraction.

    abstract：:Using a device named the cell force monitor, the contractile force developed by fibroblasts has been studied by measuring the macroscopic contraction of porous collagen-glycosaminoglycan (GAG) matrices over the first 24 h following cell attachment. In this paper, the effect of a variation in the stiffness that resists...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5408

    authors： Freyman TM,Yannas IV,Yokoo R,Gibson LJ

    更新日期：2002-01-15 00:00:00

  • Individualizing breast cancer treatment-The dawn of personalized medicine.

    abstract：:Identification of breast cancer not being a single disease but backed by multiple heterogeneous oncogenic subpopulations is of growing interest in developing personalized therapies to provide optimal outcomes. Through this review, we bring attention to evolution of tumor and microenvironment heterogeneity as a predomi...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2013.09.002

    authors： Nandy A,Gangopadhyay S,Mukhopadhyay A

    更新日期：2014-01-01 00:00:00

  • Phagocytosis of lectin-coated beads by dystrophic and normal retinal pigment epithelium.

    abstract：:In the dystrophic pigmented Royal College of Surgeons (RCS) rat, the retinal pigment epithelium (RPE) has a diminished capacity to phagocytose shed photoreceptor outer segments (ROS). An alteration in phagocytic recognition or ligand-receptor interactions between the RPE and ROS's could contribute to this defect. To t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90174-5

    authors： Seyfried-Williams R,McLaughlin BJ,Cooper NG

    更新日期：1984-10-01 00:00:00

  • Variations in signal-mediated nuclear transport during the cell cycle in BALB/c 3T3 cells.

    abstract：:Signal-mediated nuclear import was investigated in proliferating BALB/c 3T3 cells 1, 2, 4, 6, 8, 12, 18, and 21 h after the onset of anaphase. Using nucleoplasmin-coated colloidal gold particles to assay transport capacity, it was found that import was greatest at 1 h postanaphase (after complete reformation of the nu...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1333

    authors： Feldherr CM,Akin D

    更新日期：1994-11-01 00:00:00

  • A functional role for ERK in gene induction, but not in neurite outgrowth in differentiating neuroblastoma cells.

    abstract：:The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5163

    authors： Olsson AK,Nånberg E

    更新日期：2001-04-15 00:00:00

  • Stable intermediates and holdpoints in the rapid differentiation of Naegleria.

    abstract：:The rapidity of the optional 90-min differentiation of Naegleria gruberi from amoebae to flagellates suggests the possibility of a free-running cascade of events from initiating stimulus through gene expression to organelle assembly and cell morphogenesis. Instead our experiments reveal two points early in the differe...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4121

    authors： Fulton C,Lai EY

    更新日期：1998-08-01 00:00:00

  • Calpain translocation during muscle fiber necrosis and regeneration in dystrophin-deficient mice.

    abstract：:Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0227

    authors： Spencer MJ,Tidball JG

    更新日期：1996-08-01 00:00:00

  • Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice.

    abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.12.026

    authors： Deutsch U,Schlaeger TM,Dehouck B,Döring A,Tauber S,Risau W,Engelhardt B

    更新日期：2008-04-01 00:00:00

  • Effector mechanisms of fenretinide-induced apoptosis in neuroblastoma.

    abstract：:Fenretinide is an effective inducer of apoptosis in many malignancies but its precise mechanism(s) of action in the induction of apoptosis in neuroblastoma is unclear. To characterize fenretinide-induced apoptosis, neuroblastoma cell lines were treated with fenretinide and flow cytometry was used to measure apoptosis,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4988

    authors： Lovat PE,Ranalli M,Annichiarrico-Petruzzelli M,Bernassola F,Piacentini M,Malcolm AJ,Pearson AD,Melino G,Redfern CP

    更新日期：2000-10-10 00:00:00

  • Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro.

    abstract：:The formation of capillaries during development and tissue repair is likely to involve active reorganization of the actin cytoskeleton, although few studies have addressed this issue. Here, we have utilized an in vitro model of capillary morphogenesis whereby human umbilical vein endothelial cells are suspended within...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4460

    authors： Salazar R,Bell SE,Davis GE

    更新日期：1999-05-25 00:00:00

  • Distinct hydrophobic "patches" in the N- and C-tails of beta-catenin contribute to nuclear transport.

    abstract：:β-catenin is a key mediator of Wnt signaling and its deregulated nuclear accumulation can drive cancer progression. While the central armadillo (Arm) repeats of β-catenin stimulate nuclear entry, the N- and C-terminal "tail" sequences are thought to regulate turnover and transactivation. We show here that the N- and C...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.09.009

    authors： Sharma M,Jamieson C,Lui C,Henderson BR

    更新日期：2016-11-01 00:00:00

  • Phosphorylated 350 kD protein in the nucleus as it is associated with cell transformation.

    abstract：:Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90169-2

    authors： Sato C,Nishizawa K,Nakayama T,Hirai R,Nakamura H

    更新日期：1986-12-01 00:00:00

  • The expression pattern of the pyruvate dehydrogenase E1 alpha subunit genes during spermatogenesis in adult mouse.

    abstract：:The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90459-l

    authors： Takakubo F,Dahl HH

    更新日期：1992-03-01 00:00:00

  • Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles.

    abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.03.018

    authors： Moncman CL,Andrade FH

    更新日期：2010-07-15 00:00:00

  • Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues.

    abstract：:Intramuscular adipose tissue and fibrous tissue are observed in some skeletal muscle pathologies such as Duchenne muscular dystrophy and sarcopenia, and affect muscle strength and myogenesis. They originate from common fibrogenic/adipogenic cells in the skeletal muscle. Thus, elucidating the regulatory mechanisms unde...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.08.023

    authors： Takeuchi S,Nakano S,Nakamura K,Ozoe A,Chien P,Yoshihara H,Hakuno F,Matsuwaki T,Saeki Y,Takahashi S,Yamanouchi K,Nishihara M

    更新日期：2016-10-01 00:00:00

  • Nucleolin is an Ag-NOR protein; this property is determined by its amino-terminal domain independently of its phosphorylation state.

    abstract：:The Ag-NOR proteins are defined as markers of "active" ribosomal genes. They correspond to a set of proteins specifically located in the nucleolar organizer regions (NORs), but have not yet been clearly identified. We adapted the specific detection method of the Ag-NOR proteins to Western blots in order to identify th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90063-e

    authors： Roussel P,Belenguer P,Amalric F,Hernandez-Verdun D

    更新日期：1992-11-01 00:00:00

  • Toxic peptides in Frazer's fraction interact with the actin cytoskeleton and affect the targeting and function of intestinal proteins.

    abstract：:Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.06.026

    authors： Reinke Y,Zimmer KP,Naim HY

    更新日期：2009-11-15 00:00:00

  • Signal transduction in endothelial cells by the angiogenesis inhibitor histidine-rich glycoprotein targets focal adhesions.

    abstract：:Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.04.022

    authors： Lee C,Dixelius J,Thulin A,Kawamura H,Claesson-Welsh L,Olsson AK

    更新日期：2006-08-01 00:00:00

  • Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide.

    abstract：:Abdominal aortic aneurysms (AAA) are characterized by matrix remodeling, elastin degradation, absence of nitric oxide (NO) signaling, and inflammation, influencing smooth muscle cell (SMC) phenotype and gene expression. Little is known about the biomolecular release and intrinsic biomechanics of human AAA-SMCs. NO del...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111589

    authors： Farrell K,Simmers P,Mahajan G,Boytard L,Camardo A,Joshi J,Ramamurthi A,Pinet F,Kothapalli CR

    更新日期：2019-11-01 00:00:00

  • Aberrant replication of chromosomal DNA segments in CHO cells as a manifestation of a DNA repair system.

    abstract：:We have previously demonstrated that transient blocking of DNA synthesis with drugs such as 1-beta-D-arabinofuranosylcytosine results in some segments of the chromosomal DNA being replicated more than once in a single cell cycle. One explanation for this phenomenon might be that it is due to a perturbation of the cell...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90283-5

    authors： Woodcock DM,Crowther PJ,Allan G,Cooper IA

    更新日期：1983-08-01 00:00:00