Abstract：
:Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial monolayer; (ii) the enhanced interaction between senescent endothelial cells and U937 cells is mediated, at least in part, by the overexpression of ICAM-1; and (iii) LPS and interleukin 1 alpha, but not tumor necrosis factor alpha, are unable to stimulate the adhesion of U937 to senescent endothelial cells. Since monocyte adhesion to the endothelium is an early event in atherosclerosis, the altered adhesive properties observed in senescent cells could give insights into the formation of atherosclerotic lesions.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Maier JA,Statuto M,Ragnotti Gdoi
10.1006/excr.1993.1246subject
Has Abstractpub_date
1993-09-01 00:00:00pages
270-4issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(83)71246-2journal_volume
208pub_type
杂志文章abstract：:The effect of SK&F 96365 (1-(beta-[3-(4-methoxyphenyl)propoxyl]-4- methoxyphenethyl)-1H-imidazole hydrochloride), a recently synthesized inhibitor of receptor-mediated calcium entry, was investigated on human hematopoietic cell lines. We found that treatment of the T-cell leukemia line Jurkat with SK&F 96365 inhibited...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90103-f
更新日期：1992-10-01 00:00:00
abstract：:The nucleoside analogs 9-beta-D-arabinofuranosylguanine (araG) and 1-beta-d-arabinofuranosylthymine (araT) are substrates of mitochondrial nucleoside kinases and have previously been shown to be predominantly incorporated into mtDNA of cells, but the pharmacological importance of their accumulation in mtDNA is not kno...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.05.021
更新日期：2009-09-10 00:00:00
abstract：:Colorectal cancer (CRC) is characterized by a distinct metastatic pattern resembling chemokine-induced leukocyte trafficking. This prompted us to investigate expression, signal transduction and specific functions of the chemokine receptor CXCR4 in CRC cells and metastases. Using RT-PCR analysis and Western blotting, w...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.07.006
更新日期：2005-10-15 00:00:00
abstract：:Cell-matrix adhesion complexes are multi-protein structures linking the extracellular matrix (ECM) to the cytoskeleton. They are essential to both cell motility and function by bidirectionally sensing and transmitting mechanical and biochemical stimulations. Several types of cell-matrix adhesions have been identified ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2016.10.001
更新日期：2016-11-15 00:00:00
abstract：:The RAI gene is also known as iASPP and PPP1R13L. Recent investigations have shown that the region encompassing RAI is important for the development of cancer in young and middle-aged persons. It has been speculated that the RAI product induces apoptosis by blocking NF-kappaB or inhibits apoptosis by blocking p53. Eit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.05.006
更新日期：2007-07-15 00:00:00
abstract：:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.006
更新日期：2005-02-15 00:00:00
abstract：:Megakaryocytes (Mks) are unique cells in the human body in that they carry a single and polyploid nucleus. It is therefore of interest to understand their nuclear ultrastructure. PML oncogenic domains (PODs) were described in several types of eukaryotic cells using human autoantibodies which recognize nuclear antigens...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5377
更新日期：2001-12-10 00:00:00
abstract：:Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins inclu...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2013.04.009
更新日期：2013-09-10 00:00:00
abstract：:We have characterized glucocerebrosidase in various cell types of peripheral blood of control subjects and in cultured human blastoid cells. The intracellular level of glucocerebrosidase in cultured blastoid cells (10-30 nmol substrate hydrolyzed/h.mg protein) resembles closely values observed for leukocyte cell types...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90472-7
更新日期：1988-08-01 00:00:00
abstract：:In the acute promyelocytic leukemia cell line NB4, Bcl-2 downregulation occurred as a late event of retinoid-induced differentiation. In the maturation-resistant NB4-R1 subclone, retinoids failed to downregulate Bcl-2 even in the situation of apoptosis massively induced by pan-agonists and RXR-selective agonists. We o...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3594
更新日期：1997-06-15 00:00:00
abstract：:In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.08.005
更新日期：2013-11-01 00:00:00
abstract：:A great deal of evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of retinoblastoma (RB). However, the roles of lncRNA ILF3-AS1 in RB are still unclear. In the present study, our work revealed that the lncRNA ILF3-AS1 was increased in both RB tissues and cell lines. Repression of ILF3...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112087
更新日期：2020-08-15 00:00:00
abstract：:Four and one half LIM domain protein FHL2 participates in many cellular processes involved in tissue repair such as regulation of gene expression, cytoarchitecture, cell adhesion, migration and signal transduction. The repair process after wounding is initiated by the release of peptides and bioactive lipids. These mo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.09.023
更新日期：2008-12-10 00:00:00
abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1996.0362
更新日期：1996-12-15 00:00:00
abstract：:Our results showed that the expression of the src gene in chick embryo fibroblasts (CEF) released the density-dependent inhibition (DDI) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). With increasing cell density, phosphate metabolism decreased by 58% in normal CEF and, in c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90640-2
更新日期：1984-06-01 00:00:00
abstract：:In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3631
更新日期：1997-08-01 00:00:00
abstract：:Autosomal dominant polycystic kidney disease (ADPKD), a hereditary renal disease caused by mutations in PKD1 (85%) or PKD2 (15%), is characterized by the development of gradually enlarging multiple renal cysts and progressive renal failure. Polycystin-1 (PC1), PKD1 gene product, is an integral membrane glycoprotein wh...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Wnt proteins are important regulators of embryonic development, and dysregulated Wnt signalling is involved in the oncogenesis of several human cancers. Our knowledge of the downstream target genes is limited, however. We used a chromatin immunoprecipitation-based assay to isolate and characterize the actual gene segm...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.06.021
更新日期：2009-10-01 00:00:00
abstract：:Several lines of evidence indicate that telomere shortening during in vitro aging of human somatic cells plays a causal role in cellular senescence. A critical telomere length seems to be associated with the replicative block characterizing senescent cells. In this paper we analyzed the mean length of the terminal res...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4398
更新日期：1999-04-10 00:00:00
abstract：:A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90604-9
更新日期：1984-08-01 00:00:00
abstract：:Infection with Kaposi's sarcoma-associated herpesvirus (KSHV) is a prerequisite for Kaposi's sarcoma, a multicentric endothelial tumor of mixed blood vessel-lymphatic phenotype with a marked production of endothelial basement membrane proteins. The viral K1 gene encodes a unique transmembrane protein capable of transf...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.09.010
更新日期：2004-01-15 00:00:00
abstract：:Estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. Cell numbers and density were assessed in the various uterine layers. It was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, an...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90227-9
更新日期：1989-01-01 00:00:00
abstract：:The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1993.1022
更新日期：1993-02-01 00:00:00
abstract：:Cytosolic extracts from actively dividing lymphoid cells have been shown to induce DNA synthesis in isolated, quiescent nuclei. An initiating factor in such extracts (activator of DNA replication; ADR) is a > 90-kDa aprotinin-binding protein whose activity is inhibitable not only by aprotinin, but also by several othe...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1090
更新日期：1993-04-01 00:00:00
abstract：:In this communication, we have studied a 72,000 mol w (p72) host protein which reacts with a mouse monoclonal antibody (PAb6) directed against antigenic determinants on the Simian virus 40 (SV40) large T antigen protein that map 5' of 0.42 map units on the viral genome. The p72 protein is an abundant basic (pI greater...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90398-9
更新日期：1988-10-01 00:00:00
abstract：:SMN, the affected protein in spinal muscular atrophy (SMA), is a cytoplasmic protein that also occurs in nuclear structures called "gems" and is involved in snRNP maturation. Coilin-p80 is a marker protein for nuclear Cajal bodies (coiled bodies; CBs) which are also involved in snRNP maturation, storage or transport. ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5186
更新日期：2001-05-01 00:00:00
abstract：:The aging proteostasis decline manifests in a failure of aging cells and organisms to properly respond to proteotoxic challenges. This proteostasis collapse has long been considered a hallmark of aging in nematodes, and has recently been shown to occur also in human cells upon entry to senescence, opening the way to e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2021.112474
更新日期：2021-01-09 00:00:00
abstract：:A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2017.02.024
更新日期：2017-07-15 00:00:00
abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.05.008
更新日期：2008-08-01 00:00:00
abstract：:The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.02.028
更新日期：2014-05-01 00:00:00
