Mitosis-arresting effect of the calcium channel inhibitor SK&F 96365 on human leukemia cells.

abstract：:Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...

journal_title：Experimental cell research

authors： Li J,Liu G,Li L,Yao Z,Huang J

更新日期：2020-04-15 00:00:00

abstract：:A method for the in vitro perfusion of isolated guinea-pig mammary tissue is described that allows the radiolabelling of secretory and membrane proteins. Glands were depleted of methionine, labelled with [35S]methionine for 5 min and perfused with medium containing an excess of unlabelled methionine for varying times....

journal_title：Experimental cell research

authors： Mather IH,Jarasch ED,Bruder G,Heid HW,Mepham TB

更新日期：1984-03-01 00:00:00

abstract：:Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...

journal_title：Experimental cell research

authors： Bayley SA,Stones AJ,Smith CG

更新日期：1988-07-01 00:00:00

abstract：:Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...

journal_title：Experimental cell research

authors： Law AY,Lai KP,Ip CK,Wong AS,Wagner GF,Wong CK

更新日期：2008-05-01 00:00:00

abstract：:Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+,...

journal_title：Experimental cell research

authors： Lindahl PE

更新日期：1978-05-01 00:00:00

abstract：:The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

journal_title：Experimental cell research

authors： Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

更新日期：1983-12-01 00:00:00

abstract：:The expressions of the protooncogenes c-jun and jun D have been investigated in dog thyrocytes in a primary culture whose proliferation is stimulated by three distinct intracellular signaling pathways (1) the thyrotropin (TSH) or forskolin-cyclic-AMP-mediated cascade; (2) the protein kinase C pathway activated by diac...

journal_title：Experimental cell research

authors： Reuse S,Pirson I,Dumont JE

更新日期：1991-10-01 00:00:00

abstract：:Template-activating factors I (TAF-I) alpha and beta have been identified as chromatin remodeling factors from human HeLa cells. TAF-I beta corresponds to the protein encoded by the set gene, which was found in an acute undifferentiated leukemia as a fusion version with the can gene via chromosomal translocation. To d...

journal_title：Experimental cell research

authors： Nagata K,Saito S,Okuwaki M,Kawase H,Furuya A,Kusano A,Hanai N,Okuda A,Kikuchi A

更新日期：1998-05-01 00:00:00

abstract：:Chromosomal DNA fiber replication was investigated in the shoot meristem of mustard plants during the morphogenetic transition from the leaf-forming (vegetative) to the flower-forming (evoked) condition. The replicon size, determined using the modal class, was 15 micron in the vegetative meristem and shifted to 7.5 mi...

journal_title：Experimental cell research

authors： Jacqmard A,Houssa C

更新日期：1988-12-01 00:00:00

abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

journal_title：Experimental cell research

authors： Lang C,Wang J,Chen L

更新日期：2017-11-15 00:00:00

abstract：:The formation of capillaries during development and tissue repair is likely to involve active reorganization of the actin cytoskeleton, although few studies have addressed this issue. Here, we have utilized an in vitro model of capillary morphogenesis whereby human umbilical vein endothelial cells are suspended within...

journal_title：Experimental cell research

authors： Salazar R,Bell SE,Davis GE

更新日期：1999-05-25 00:00:00

abstract：:Megakaryocytes (Mks) are unique cells in the human body in that they carry a single and polyploid nucleus. It is therefore of interest to understand their nuclear ultrastructure. PML oncogenic domains (PODs) were described in several types of eukaryotic cells using human autoantibodies which recognize nuclear antigens...

journal_title：Experimental cell research

authors： Drouin A,Schmitt A,Massé JM,Cieutat AM,Fichelson S,Cramer EM

更新日期：2001-12-10 00:00:00

abstract：:A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...

journal_title：Experimental cell research

authors： Cavadas MAS,Cheong A,Taylor CT

更新日期：2017-07-15 00:00:00

abstract：:We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectiv...

journal_title：Experimental cell research

authors： Bouniol-Baly C,Nguyen E,Besombes D,Debey P

更新日期：1997-10-10 00:00:00

abstract：:The level of integrin alpha(v)beta3 and its ligand osteopontin (OPN) has been directly correlated to tumorigenicity of melanoma and other cancer cells. We have previously shown an increase in pp(60c-Src) kinase activity associated with integrin alpha(v)beta3 in melanoma cells (M21) treated with soluble OPN. pp(60c-Src...

journal_title：Experimental cell research

authors： Samanna V,Wei H,Ego-Osuala D,Chellaiah MA

更新日期：2006-07-15 00:00:00

abstract：:Tumor endothelial marker (TEM) 5 is an adhesion G-protein-coupled receptor upregulated in endothelial cells during tumor and physiologic angiogenesis. So far, the mechanisms leading to upregulation of TEM5 and its function during angiogenesis have not been identified. Here, we report that TEM5 expression in endothelia...

journal_title：Experimental cell research

authors： Vallon M,Rohde F,Janssen KP,Essler M

更新日期：2010-02-01 00:00:00

abstract：:Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment establ...

journal_title：Experimental cell research

authors： Tacchetti C,Tavella S,Dozin B,Quarto R,Robino G,Cancedda R

更新日期：1992-05-01 00:00:00

abstract：:Adhesion of hepatocytes to collagenous substrates and their spreading have been shown to involve a specific recognition event, possibly mediated by membrane proteins with affinity for collagen. In the present communication, we describe the isolation of membrane components that are involved in the adhesion of rat hepat...

journal_title：Experimental cell research

authors： Rubin K,Gullberg D,Borg TK,Obrink B

更新日期：1986-05-01 00:00:00

abstract：:Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the ...

journal_title：Experimental cell research

authors： Iordache C,Duszyk M

更新日期：2007-01-15 00:00:00

abstract：:Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the i...

journal_title：Experimental cell research

authors： Twomey E,Li Y,Lei J,Sodja C,Ribecco-Lutkiewicz M,Smith B,Fang H,Bani-Yaghoub M,McKinnell I,Sikorska M

更新日期：2010-01-01 00:00:00

abstract：:We have studied the internalization of targeted fusogenic liposome content to leukemic T cells (CEM) in vitro. We describe a method for the covalent coupling of T101 antibody to the surface of liposomes and the incorporation of fusogenic viral protein into the liposome membrane. Hygromycin B, an impermeant inhibitor o...

journal_title：Experimental cell research

authors： Sechoy O,Vidal M,Philippot JR,Bienvenue A

更新日期：1989-11-01 00:00:00

abstract：:Retinol binding protein (RBP) is the plasma transport protein of retinol. Mobilization of RBP from the liver stores is stimulated by retinol. During vitamin A deficiency, RBP secretion is specifically inhibited while its rate of biosynthesis is unaffected. As a consequence, RBP, as apoprotein, accumulates inside the e...

journal_title：Experimental cell research

authors： Tosetti F,Ferrari N,Pfeffer U,Brigati C,Vidali G

更新日期：1992-06-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：:Protein kinase B (Akt1) holds a central role for cellular growth, development, and survival, but the cellular pathways of Akt1 that prevent inflammatory demise in the vascular system remain undefined. Employing a constitutively active form of Akt1 (myristoylated Akt1) in endothelial cells (ECs), we demonstrate that Ak...

journal_title：Experimental cell research

authors： Chong ZZ,Kang JQ,Maiese K

更新日期：2004-06-10 00:00:00

abstract：:The radula of the limpet, Cellana toreuma, consists of a continuous series of teeth in various stages of iron biomineralization. The major iron-binding protein of the limpet's iron-containing granule (siderosome) has been purified and identified as ferritin. Limpet ferritin has a M(r) of 575 kDa and can be resolved in...

journal_title：Experimental cell research

authors： Lu HK,Huang CM,Li CW

更新日期：1995-07-01 00:00:00

abstract：:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...

journal_title：Experimental cell research

authors： Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C

更新日期：1996-03-15 00:00:00

abstract：:RNA polymerase II transcripts are confined to nuclear compartments. A detailed analysis of the nuclear topology of RNA from individual genes was performed for transcripts from the marker gene coding for chloramphenicol acetyltransferase, expressed at a high level from the HTLV-1 LTR promoter. The construct was transfe...

journal_title：Experimental cell research

authors： Bridger JM,Kalla C,Wodrich H,Weitz S,King JA,Khazaie K,Kräusslich HG,Lichter P

更新日期：2005-01-15 00:00:00

abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

journal_title：Experimental cell research

authors： Ledermann B,Bürki K

更新日期：1991-12-01 00:00:00

abstract：:Insulin mimetics, including zinc containing compounds, have previously been shown to influence chondrogenesis as it relates to healing of fractures in various preclinical models. However, the mechanism by which these compounds drive chondrogenic differentiation is yet undefined. Here, via next-generation sequencing (N...

journal_title：Experimental cell research

authors： Hozain S,Hernandez A,Fuller J,Sharp G,Cottrell J

更新日期：2021-01-21 00:00:00

abstract：:The aim of this study was to assess the effect of different culture conditions on the survival and morphological phenotype of cultured acinar cells. Acinar fragments isolated from hamster pancreas were embedded in rat-tail collagen. Four groups were established: Medium 1-5% NuSerum + basic medium (basic medium = DMEM/...

journal_title：Experimental cell research

authors： Yuan S,Duguid WP,Agapitos D,Wyllie B,Rosenberg L

更新日期：1997-12-15 00:00:00