Guidance and activation of murine macrophages by nanometric scale topography.


:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activated cell spreading and adhesion and increased the number of protrusions of the cell membrane. These changes were accompanied by an increase in the amount of F-actin in cells. The accumulation of F-actin and vinculin in cells was observed along the edges of single steps or grooves. Formation of focal contacts along discontinuities in the substratum was accompanied by the phosphorylation of tyrosine colocalized with F-actin and vinculin. A similar pattern of staining was seen in cells stained for vitronectin receptor, alphaV integrin, but not for integrins alpha5beta1 or alpha3beta1. Cells cultured on nanogrooves showed a higher phagocytotic activity than cells cultured on plain substrata. We show that macrophages can react to ultrafine features of topography of a size comparable to that of a single collagen fiber and become activated by the contact with topographic features.


Exp Cell Res


Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C




Has Abstract


1996-03-15 00:00:00














  • Spermatogenesis in mice is not affected by histone H1.1 deficiency.

    abstract::The linker histone subtype H1.1 belongs to the group of main-type histones and is synthesized in somatic tissues as well as in germ cells during the S phase of the cell cycle. In adult mice the histone gene H1.1 is expressed mainly in thymus, spleen, and testis. The single-copy gene coding for the H1.1 protein was eli...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rabini S,Franke K,Saftig P,Bode C,Doenecke D,Drabent B

    更新日期:2000-02-25 00:00:00

  • Changes in the DNA polymerase beta gene expression during development of lung, brain, and testis suggest an involvement of the enzyme in DNA recombination.

    abstract::Changes in the expression pattern of DNA polymerase beta gene during rat lung, brain, and testis development have been investigated. A decrease in the level of beta-pol mRNA was observed during postnatal development of lung and brain. By contrast, an almost 20-fold increase in the level of beta-pol mRNA was observed d...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nowak R,Woszczynski M,Siedlecki JA

    更新日期:1990-11-01 00:00:00

  • Transforming growth factor-beta activates both pro-apoptotic and survival signals in fetal rat hepatocytes.

    abstract::Transforming growth factor-beta (TGF-beta) induces apoptosis in fetal rat hepatocytes. However, a subpopulation of these cells survives concomitant with changes in morphology and phenotype, reminiscent of an epithelial mesenchymal transition (EMT) [Exp. Cell Res. 252 (1999) 281-291]. In this work, we have isolated the...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Valdés F,Murillo MM,Valverde AM,Herrera B,Sánchez A,Benito M,Fernández M,Fabregat I

    更新日期:2004-01-01 00:00:00

  • Transmyocardial drilling revascularization combined with heparinized bFGF-incorporating stent activates resident cardiac stem cells via SDF-1/CXCR4 axis.

    abstract:OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhang GW,Wen T,Gu TX,Li-Ling J,Wang C,Zhao Y,Liu J,Wang Y,Liu TJ,Lü F

    更新日期:2012-02-15 00:00:00

  • IgG binding to cytoskeletal intermediate filaments activates the complement cascade.

    abstract::The cellular plasma membrane becomes permeable to macromolecules during the cell injury process. This results in exposure of the interior of the cell to plasma proteins and to high-affinity binding of the Fc part of IgG to intermediate filaments (Hansson, G K, Starkebaum, G A, Benditt, E P & Schwartz, S M, Proc natl a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hansson GK,Lagerstedt E,Bengtsson A,Heideman M

    更新日期:1987-06-01 00:00:00

  • The tumor suppressor protein p16INK4a.

    abstract::The tumor suppressor protein p16INK4a (inhibitor of CDK4) is one of the most direct links between cell-cycle control and cancer. The p16INK4a gene is frequently inactivated in human tumors, and inheritance of mutant alleles results in susceptibility to several types of cancer. p16INK4a is part of a cell-cycle regulato...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Serrano M

    更新日期:1997-11-25 00:00:00

  • Inhibition of topoisomerase II activity and its effect on nucleolar structure and function.

    abstract::The relationship between topoisomerase II activity and ribosomal RNA synthesis was investigated using the antitumoral drug VM26, a specific inhibitor of topoisomerase II. For this purpose TG cells, a human tumor cell line, were cultured in the presence of 2.5 microM VM26 for 1 and 3 h; VM26 reduced the topoisomerase I...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Govoni M,Farabegoli F,Pession A,Novello F

    更新日期:1994-03-01 00:00:00

  • Shp-2 tyrosine phosphatase: signaling one cell or many.

    abstract::Shp-2, a widely expressed cytoplasmic tyrosine phosphatase with two src-homology 2 (SH2) domains, has received much attention in the signal transduction field recently. Significant progress has been made in understanding the structure and function of this phosphatase, together with its Drosophila homologue, Corkscrew,...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Feng GS

    更新日期:1999-11-25 00:00:00

  • Distinct functions for Rap1 signaling in vascular morphogenesis and dysfunction.

    abstract::Rap1 signaling is important for both major processes of vessel formation: vasculogenesis, or de novo vessel formation, and angiogenesis, sprouting of new vessels from pre-existing ones. We provide an overview of genetic studies in mice and zebrafish and discuss some of the proposed underlying mechanisms derived from c...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Chrzanowska-Wodnicka M

    更新日期:2013-09-10 00:00:00

  • Embryonic lineage of vascular smooth muscle cells determines responses to collagen matrices and integrin receptor expression.

    abstract::Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist TH

    更新日期:1996-08-25 00:00:00

  • Identification of the type-B receptor for platelet-derived growth factor in human embryonal carcinoma cells.

    abstract::The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

    更新日期:1990-02-01 00:00:00

  • A simple technique for quantitation of low levels of DNA damage in individual cells.

    abstract::Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with H2O2 (9.1 to 291 microM) at 4 degrees C and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Both agents induced a significant increase in DNA migration, beg...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Singh NP,McCoy MT,Tice RR,Schneider EL

    更新日期:1988-03-01 00:00:00

  • Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.

    abstract::On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ohno T,Kako R,Sato C,Ohkawa A

    更新日期:1986-04-01 00:00:00

  • TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner.

    abstract::Satellite cells are a quiescent heterogeneous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-beta (TGF-beta) superfamily members are elevated post-injury and their importance in the r...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schabort EJ,van der Merwe M,Loos B,Moore FP,Niesler CU

    更新日期:2009-02-01 00:00:00

  • Hepatocyte growth factor (HGF) enhances cardiac commitment of differentiating embryonic stem cells by activating PI3 kinase.

    abstract::Hepatocyte growth factor (HGF) is a pleiotropic cytokine promoting proliferation, migration and survival in several cell types. HGF and its cognate receptor c-Met are expressed in cardiac cells during early cardiogenesis, but data concerning its role in cardiac differentiation of embryonic stem cells (ESCs) and the un...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Roggia C,Ukena C,Böhm M,Kilter H

    更新日期:2007-03-10 00:00:00

  • Thyroid hormone enhancement of estradiol stimulation of breast carcinoma proliferation.

    abstract::Thyroid hormone (T3) and estradiol (Est) modulate biological processes by binding to nuclear receptor proteins that, through interactions with specific response elements in the regulatory regions of genes, modulate gene transcription. Est stimulation of estrogen receptor (ER)-positive breast carcinoma cell growth occu...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shao ZM,Sheikh MS,Rishi AK,Dawson MI,Li XS,Wilber JF,Feng P,Fontana JA

    更新日期:1995-05-01 00:00:00

  • On and off: proteasome and TGF-beta signaling.

    abstract::Transforming growth factor-beta (TGF-beta) signaling mainly relies on the TGF-beta receptor-Smad pathway. Meanwhile, TGF-beta binding to its receptors initiates the degradation of several key components of its signaling pathway. The degradation of these components, including both positive and negative transducers, is ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Zhang F,Laiho M

    更新日期:2003-12-10 00:00:00

  • Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells.

    abstract::Tunneling nanotubes (TnTs) are long, non-adherent, actin-based cellular extensions that act as conduits for transport of cellular cargo between connected cells. The mechanisms of nanotube formation and the effects of the tumor microenvironment and cellular signals on TnT formation are unknown. In the present study, we...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thayanithy V,Babatunde V,Dickson EL,Wong P,Oh S,Ke X,Barlas A,Fujisawa S,Romin Y,Moreira AL,Downey RJ,Steer CJ,Subramanian S,Manova-Todorova K,Moore MAS,Lou E

    更新日期:2014-04-15 00:00:00

  • Differentiation-linked expression of prothymosin alpha gene in human myeloid leukemic cells.

    abstract::Prothymosin alpha (ProT alpha) is a nuclear protein related to cell proliferation. Its gene is highly activated during postnatal development at stages containing many proliferating but also differentiating cells. In this report, a study on ProT alpha gene expression during differentiation of human myeloid leukemic (HL...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dosil M,Alvarez-Fernández L,Gómez-Márquez J

    更新日期:1993-01-01 00:00:00

  • Subcellular distribution of distinct nucleolin subfractions recognized by two monoclonal antibodies.

    abstract::Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schwab MS,Gossweiler U,Dreyer C

    更新日期:1998-03-15 00:00:00

  • Shape of my heart: Cell-cell adhesion and cytoskeletal dynamics during Drosophila cardiac morphogenesis.

    abstract::The fruit fly Drosophila melanogaster has recently emerged as an excellent system to investigate the genetics of cardiovascular development and disease. Drosophila provides an inexpensive and genetically-tractable in vivo system with a large number of conserved features. In addition, the Drosophila embryo is transpare...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: McFaul CMJ,Fernandez-Gonzalez R

    更新日期:2017-09-01 00:00:00

  • Morphology of endoplasmic reticulum and Golgi elements following microinjection of rat liver microsomes into Xenopus laevis oocyte cytoplasm.

    abstract::Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Paiement J

    更新日期:1986-10-01 00:00:00

  • Cleavage of Bax enhances its cell death function.

    abstract::Members of the Bcl-2 family of proteins are key regulators of apoptosis. Some of these proteins undergo posttranslational modification, such as phosphorylation or proteolysis, that serves to alter their function. Caspases are known to cleave Bid, a proapoptotic family member, as well as Bcl-2 and Bcl-X(L), two prosurv...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wood DE,Newcomb EW

    更新日期:2000-05-01 00:00:00

  • Fibroblast growth factors 1 and 2 inhibit adipogenesis of human bone marrow stromal cells in 3D collagen gels.

    abstract::Multipotent human bone marrow stromal cells (hBMSCs) are the common progenitors of osteoblasts and adipocytes. A shift in hBMSC differentiation in favor of adipogenesis may contribute to the bone loss and marrow fat accumulation observed in aging and osteoporosis. Hence, the identification of factors modulating marrow...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Le Blanc S,Simann M,Jakob F,Schütze N,Schilling T

    更新日期:2015-11-01 00:00:00

  • Cell cycle-dependent DNA damage signaling induced by ICRF-193 involves ATM, ATR, CHK2, and BRCA1.

    abstract::Topoisomerase II is essential for cell proliferation and survival and has been a target of various anticancer drugs. ICRF-193 has long been used as a catalytic inhibitor to study the function of topoisomerase II. Here, we show that ICRF-193 treatment induces DNA damage signaling. Treatment with ICRF-193 induced G2 arr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Park I,Avraham HK

    更新日期:2006-07-01 00:00:00

  • Effects of polyamines on the first division cycle of Xenopus laevis eggs.

    abstract::The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grant NJ,Aimar C,Oriol-Audit C

    更新日期:1984-02-01 00:00:00

  • MUC6 mucin expression inhibits tumor cell invasion.

    abstract::The MUC6 mucin has a critical protective function in the normal stomach, pancreas and duodenum and is aberrantly expressed during the progression of some gastrointestinal cancers. Our aim was to determine whether MUC6 contributes to the etiology or progression of pancreatic cancer and elucidate the molecular basis of ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Leir SH,Harris A

    更新日期:2011-10-15 00:00:00

  • The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes.

    abstract::LMX1B is a LIM-homeodomain transcription factor essential for development. Putative LMX1B target genes have been identified through mouse gene targeting studies, but their identity as direct LMX1B targets remains hypothetical. We describe here the first molecular characterization of LMX1B target gene regulation. Micro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rascle A,Neumann T,Raschta AS,Neumann A,Heining E,Kastner J,Witzgall R

    更新日期:2009-01-01 00:00:00

  • Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25.

    abstract::Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Binger T,Stich S,Andreas K,Kaps C,Sezer O,Notter M,Sittinger M,Ringe J

    更新日期:2009-05-01 00:00:00

  • HRP-2, a heterogeneous nuclear ribonucleoprotein, is essential for embryogenesis and oogenesis in Caenorhabditis elegans.

    abstract::Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kinnaird JH,Maitland K,Walker GA,Wheatley I,Thompson FJ,Devaney E

    更新日期:2004-08-15 00:00:00