Guidance and activation of murine macrophages by nanometric scale topography.


:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activated cell spreading and adhesion and increased the number of protrusions of the cell membrane. These changes were accompanied by an increase in the amount of F-actin in cells. The accumulation of F-actin and vinculin in cells was observed along the edges of single steps or grooves. Formation of focal contacts along discontinuities in the substratum was accompanied by the phosphorylation of tyrosine colocalized with F-actin and vinculin. A similar pattern of staining was seen in cells stained for vitronectin receptor, alphaV integrin, but not for integrins alpha5beta1 or alpha3beta1. Cells cultured on nanogrooves showed a higher phagocytotic activity than cells cultured on plain substrata. We show that macrophages can react to ultrafine features of topography of a size comparable to that of a single collagen fiber and become activated by the contact with topographic features.


Exp Cell Res


Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C




Has Abstract


1996-03-15 00:00:00














  • Cell-surface location and molecular properties of cell-CAM 105 in intestinal epithelial cells.

    abstract::Cell-CAM 105 is involved in intercellular adhesion of isolated rat hepatocytes in vitro. In addition to liver, cell-CAM 105 occurs in several different epithelia, in platelets, and in granulocytes. In this paper we present a detailed analysis of cell-CAM 105 in the small intestinal mucosa. Isolated rat intestinal epit...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hansson M,Blikstad I,Obrink B

    更新日期:1989-03-01 00:00:00

  • An altered repertoire of fos/jun (AP-1) at the onset of replicative senescence.

    abstract::With multiple divisions in culture, normal diploid cells suffer a loss of growth potential that leads to replicative senescence and a finite replicative capacity. Using quantitative RT-PCR, we have monitored mRNA expression levels of c-fos, c-jun, JunB, c-myc, p53, H-ras, and histone H4 during the replicative senescen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Irving J,Feng J,Wistrom C,Pikaart M,Villeponteau B

    更新日期:1992-09-01 00:00:00

  • Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cells.

    abstract::Cultured arterial smooth muscle cells incorporate [35S]sulfate into the extracellular chondroitin sulfate/dermatan sulfate containing proteoglycans at a higher rate in the phase of logarithmic growth than do non-dividing cells. The cell growth-dependent decrease in 35S incorporation with increasing cell density is acc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hollmann J,Thiel J,Schmidt A,Buddecke E

    更新日期:1986-12-01 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract::A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Moore SA

    更新日期:1987-08-01 00:00:00

  • 1,25-dihydroxyvitamin D3 induces biphasic NF-kappaB responses during HL-60 leukemia cells differentiation through protein induction and PI3K/Akt-dependent phosphorylation/degradation of IkappaB.

    abstract::1,25-dihydroxyvitamin D(3) (VD(3)) induces differentiation in a number of leukemia cell lines and under various conditions is able to either stimulate or inhibit nuclear factor kappa B (NF-kappaB) activity. Here we report a time-dependent biphasic regulation of NF-kappaB in VD(3)-treated HL-60 leukemia cells. After VD...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tse AK,Wan CK,Shen XL,Zhu GY,Cheung HY,Yang M,Fong WF

    更新日期:2007-05-01 00:00:00

  • The protein tyrosine phosphatase SHP-2 is required for EGFRvIII oncogenic transformation in human glioblastoma cells.

    abstract::Oncogenic EGFRvIII is a naturally occurring oncoprotein and is expressed in about 40-50% of human glioblastomas, particularly those that arise de novo. To understand the molecular mechanisms by which this oncoprotein alters transforming phenotypes, and since our previous work indicated that SHP-2 protein tyrosine phos...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhan Y,Counelis GJ,O'Rourke DM

    更新日期:2009-08-15 00:00:00

  • Establishment and characterization of an epithelial intestinal cell line from rat fetus.

    abstract::An epithelial intestinal cell line has been established from explants of fetal rat small intestine. After the 9th passage (approx. 25 population doublings) epithelial-like cells acquired the properties of a permanent cell line. The epithelial nature of this cell line, and of clone IRD 98 subsequently isolated, is supp...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Négrel R,Rampal P,Nano JL,Cavenel C,Ailhaud G

    更新日期:1983-02-01 00:00:00

  • Improvement of calcium handling and changes in calcium-release properties after mini- or full-length dystrophin forced expression in cultured skeletal myotubes.

    abstract::Dystrophin is a cytoskeletal protein normally expressed underneath the sarcolemma of muscle fibers. The lack of dystrophin in Duchenne muscular Dystrophy (DMD) muscles results in fiber necrosis, which was proposed to be mediated by chronic calcium mishandling. The extensive comparison of dystrophic cells from human or...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Marchand E,Constantin B,Balghi H,Claudepierre MC,Cantereau A,Magaud C,Mouzou A,Raymond G,Braun S,Cognard C

    更新日期:2004-07-15 00:00:00

  • Epigenetic silencing in embryogenesis.

    abstract::Transcription profiling of early embryos emphasizes that differential gene expression is a fundamental control mechanism of development. Precise regulatory mechanisms operate on a background of epigenetic changes in chromatin composition, modification, and architecture that are integral for the development of a plurip...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Meehan RR,Dunican DS,Ruzov A,Pennings S

    更新日期:2005-10-01 00:00:00

  • Regulation of p53 in embryonic stem cells.

    abstract::Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p5...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Solozobova V,Blattner C

    更新日期:2010-09-10 00:00:00

  • Pivotal role of the RB family proteins in in vitro thyroid cell transformation.

    abstract::Rat thyroid differentiated cells (PC Cl 3) are an excellent model system with which to study the interaction between differentiation and cell transformation. We previously demonstrated that PC Cl 3 cells expressing the adenovirus E1A gene no longer depend on thyrotropin for growth and do not express thyroid differenti...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Iuliano R,Trapasso F,Stella A,Le Pera I,Melillo RM,Bruni P,Baldassarre G,Chiariotti L,Santoro M,Viglietto G,Fusco A

    更新日期:2000-11-01 00:00:00

  • Mitf regulates osteoclastogenesis by modulating NFATc1 activity.

    abstract::Transcription factors Mitf and NFATc1 share many downstream targets that are critical for osteoclastogenesis. Since RANKL signals induce/activate both NFATc1 and Mitf isoform-E (Mitf-E), a tissue-restricted Mitf isoform in osteoclasts, it is plausible that the two factors work together to promote osteoclastogenesis. A...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lu SY,Li M,Lin YL

    更新日期:2014-10-15 00:00:00

  • Exemption of satellite DNA from demethylation in immortalized differentiated derivatives of F9 mouse embryonal carcinoma cells.

    abstract::DNA methylation in F9 embryonal carcinoma cells at various stages of retinoic acid-induced differentiation was compared to that in immortalized differentiated derivatives of this cell line. Different repetitive sequences, such as L1 LINE, GAPDH pseudogenes, and major and minor satellite DNA, lost their methylation to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Teubner B,Schulz WA

    更新日期:1994-02-01 00:00:00

  • Functional conservation of 14-3-3 isoforms in inhibiting bad-induced apoptosis.

    abstract::14-3-3 proteins are a family of homologous eukaryotic molecules with seven distinct isoforms in mammalian cells. Isoforms of 14-3-3 proteins interact with diverse ligands and are involved in the regulation of mitogenesis, cell cycle progression, and apoptosis. However, whether different 14-3-3 isoforms are responsible...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Subramanian RR,Masters SC,Zhang H,Fu H

    更新日期:2001-11-15 00:00:00

  • Embryonic lineage of vascular smooth muscle cells determines responses to collagen matrices and integrin receptor expression.

    abstract::Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist TH

    更新日期:1996-08-25 00:00:00

  • Intronic miR-932 targets the coding region of its host gene, Drosophila neuroligin2.

    abstract::Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Qian J,Tu R,Yuan L,Xie W

    更新日期:2016-06-10 00:00:00

  • Long noncoding RNA CCAT2 promotes hepatocellular carcinoma proliferation and metastasis through up-regulation of NDRG1.

    abstract:BACKGROUND:Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liu Y,Wang D,Li Y,Yan S,Dang H,Yue H,Ling J,Chen F,Zhao Y,Gou L,Tang P,Huang A,Tang H

    更新日期:2019-06-01 00:00:00

  • Esophageal epithelial and mesenchymal cross-talk leads to features of epithelial to mesenchymal transition in vitro.

    abstract:BACKGROUND:Esophageal fibrosis is a complication of eosinophilic esophagitis (EoE) which has been attributed to both subepithelial fibrosis and to epithelial to mesenchymal transition (EMT), a process by which epithelial cells acquire mesenchymal features. Common to both causes of EoE-fibrosis is the notion that granul...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Muir AB,Lim DM,Benitez AJ,Modayur Chandramouleeswaran P,Lee AJ,Ruchelli ED,Spergel JM,Wang ML

    更新日期:2013-04-01 00:00:00

  • Caspase activity is not sufficient to execute cell death.

    abstract::Molecular studies of the physiological cell death process have focused attention on the role of effector caspases as critical common elements of the lethal mechanism. Diverse death signals act afferently via distinct signaling pathways to activate these resident proenzyme molecules post-translationally. Whether this m...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lukovic D,Komoriya A,Packard BZ,Ucker DS

    更新日期:2003-10-01 00:00:00

  • Structure in the amphibian germinal vesicle.

    abstract::The germinal vesicle (GV) of Xenopus laevis is an enormous nucleus that contains 18 giant lampbrush chromosomes and thousands of inclusions. The inclusions are primarily of three types: approximately 1500 extrachromosomal nucleoli, 50-100 Cajal bodies, and several thousand B-snurposomes, which correspond to speckles o...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Gall JG,Wu Z,Murphy C,Gao H

    更新日期:2004-05-15 00:00:00

  • Growth stimulation of rat primary embryo fibroblasts by the human myc gene.

    abstract::We have studied the ability of plasmids encoding a normal human myc protein to stimulate growth of primary rat embryo fibroblasts. We measured growth stimulation by the number of G418-resistant colonies obtained after co-transfection with plasmid pSV2neo and by the percentage of these colonies that grew in long-term c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nicolaiew N,Dautry F

    更新日期:1986-10-01 00:00:00

  • Endothelial monocyte activating polypeptide-II modulates endothelial cell responses by degrading hypoxia-inducible factor-1alpha through interaction with PSMA7, a component of the proteasome.

    abstract::The majority of human tumors are angiogenesis dependent. Understanding the specific mechanisms that contribute to angiogenesis may offer the best approach to develop therapies to inhibit angiogenesis in cancer. Endothelial monocyte activating polypeptide-II (EMAP-II) is an anti-angiogenic cytokine with potent effects ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tandle AT,Calvani M,Uranchimeg B,Zahavi D,Melillo G,Libutti SK

    更新日期:2009-07-01 00:00:00

  • Bone morphogenetic protein -4 and -5 in pancreatic cancer--novel bidirectional players.

    abstract::Bone morphogenetic proteins (BMPs) are multifunctional signaling molecules that have gained increasing interest in cancer research. To obtain a systematic view on BMP signaling in pancreatic cancer we first determined the mRNA expression levels of seven BMP ligands (BMP2-BMP8) and six BMP specific receptors in pancrea...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Virtanen S,Alarmo EL,Sandström S,Ampuja M,Kallioniemi A

    更新日期:2011-09-10 00:00:00

  • Differential regulation of thyrotropin receptor and thyroglobulin mRNA accumulation at the cellular level: an in situ hybridization study.

    abstract::Regulation of TSH receptor (TSHr) mRNA accumulation has been investigated in canine thyrocytes in primary culture by in situ hybridization experiments; the effects of the mitogenic thyrotropin (TSH), epidermal growth factor (EGF), and phorbol ester TPA (12-O-tetradecanoylphorbol-13-acetate) have been compared. Apart f...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pohl V,Maenhaut C,Gérard C,Vassart G,Dumont JE

    更新日期:1992-04-01 00:00:00

  • The effect of a 10-day space flight on the function, phenotype, and adhesion molecule expression of splenocytes and lymph node lymphocytes.

    abstract::Previous studies have indicated that space flight affects the activation of lymphocytes from humans, monkeys, and rodents. In rats, where lymphocytes from blood, spleen, and lymph nodes have been tested, the accumulated data suggest that the effects of flight on various cells are lymphoid organ-specific. Thus, cells m...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grove DS,Pishak SA,Mastro AM

    更新日期:1995-07-01 00:00:00

  • Diacylglycerol kinase-theta is localized in the speckle domains of the nucleus.

    abstract::It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tabellini G,Bortul R,Santi S,Riccio M,Baldini G,Cappellini A,Billi AM,Berezney R,Ruggeri A,Cocco L,Martelli AM

    更新日期:2003-07-01 00:00:00

  • Murine embryonal carcinoma cell-surface sialyl LeX is present on a novel glycoprotein and on high-molecular-weight lactosaminoglycan.

    abstract::Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rosenman SJ,Fenderson BA,Hakomori SI

    更新日期:1989-02-01 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract::Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhang J,Harrison JS,Studzinski GP

    更新日期:2011-01-01 00:00:00

  • Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking.

    abstract::Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Massol RH,Larsen JE,Kirchhausen T

    更新日期:2005-05-15 00:00:00

  • Zinc finger protein 593 is upregulated during skeletal muscle atrophy and modulates muscle cell differentiation.

    abstract::Skeletal muscle atrophy is a debilitating condition that can arise due to aging, cancer, corticosteroid use, and denervation. To better characterize the molecular genetic events of neurogenic atrophy, a previous study analyzed gene expression patterns in gastrocnemius muscle following sciatic nerve transection and fou...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lynch SA,McLeod MA,Orsech HC,Cirelli AM,Waddell DS

    更新日期:2019-10-15 00:00:00