Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation.

abstract：:Phosphorylation of types III and IV intermediate filaments (IFs) is known to regulate their organization and function. Phosphorylation of the amino-terminal head domain sites on types III and IV IF proteins plays a key role in the assembly/disassembly of IF subunits into 10 nm filaments, and influences the phosphoryla...

journal_title：Experimental cell research

authors： Sihag RK,Inagaki M,Yamaguchi T,Shea TB,Pant HC

更新日期：2007-06-10 00:00:00

abstract：:Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nu...

journal_title：Experimental cell research

authors： Skare P,Kreivi JP,Bergström A,Karlsson R

更新日期：2003-05-15 00:00:00

abstract：:Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

journal_title：Experimental cell research

authors： Massol RH,Larsen JE,Kirchhausen T

更新日期：2005-05-15 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：:Phosphoinositide 3-kinases (PI3Ks) are an evolutionary conserved family of lipid kinases that control cell growth, metabolism and survival. By generating lipid second messengers that interact with specialized lipid-binding domains found in a wide spectrum of signaling molecules, PI3Ks instigate signaling through a net...

journal_title：Experimental cell research

authors： Graupera M,Potente M

更新日期：2013-05-15 00:00:00

abstract：:During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for...

journal_title：Experimental cell research

authors： Bryantsev AL,Chechenova MB,Shelden EA

更新日期：2007-01-01 00:00:00

abstract：:Hyaluronan binding protein (HABP1), located on human chromosome 17p13.3, was identified and characterized as being involved in cellular signaling from our laboratory. Here, we demonstrate that HABP1 expression in Schizosaccharomyces pombe induces growth inhibition, morphological abnormalities like elongation, multinuc...

journal_title：Experimental cell research

authors： Mallick J,Datta K

更新日期：2005-10-01 00:00:00

abstract：:Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...

journal_title：Experimental cell research

authors： Gentile MT,Russo R,Pastorino O,Cioffi S,Barbieri F,Illingworth EA,Grieco M,Chambery A,Colucci-D'Amato L

更新日期：2018-03-01 00:00:00

abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...

journal_title：Experimental cell research

authors： Lv N,Kim EK,Song MY,Choi HN,Moon WS,Park SJ,Park JW,Kwon KB,Park BH

更新日期：2009-07-15 00:00:00

abstract：:The relationship between topoisomerase II activity and ribosomal RNA synthesis was investigated using the antitumoral drug VM26, a specific inhibitor of topoisomerase II. For this purpose TG cells, a human tumor cell line, were cultured in the presence of 2.5 microM VM26 for 1 and 3 h; VM26 reduced the topoisomerase I...

journal_title：Experimental cell research

authors： Govoni M,Farabegoli F,Pession A,Novello F

更新日期：1994-03-01 00:00:00

abstract：:We had identified earlier a germ cell-specific lamin of 60 kDa in rat which is related to somatic lamin B. This polypeptide was shown to be the only major component organizing the lamina structure of round spermatids. In the present study, we find that this 60-kDa polypeptide persists in the testicular and epididymal ...

journal_title：Experimental cell research

authors： Sudhakar L,Sivakumar N,Behal A,Rao MR

更新日期：1992-01-01 00:00:00

abstract：:Galectin-3, a beta-galactoside-binding protein, has been shown to be involved in multiple biological processes through interaction with its complementary glycoconjugates. Here we provide the first evidence of galectin-3 as a mitogen. Incubation of quiescent cultures of normal human lung fibroblast IMR-90 cells with re...

journal_title：Experimental cell research

authors： Inohara H,Akahani S,Raz A

更新日期：1998-12-15 00:00:00

abstract：:Using the restriction enzymes MspI and HpaII in the nick translation procedure it has been shown that decondensation of the paracentric heterochromatin of chromosome 9 during human spermatogenesis is associated with hypomethylation of the DNA sequences in this domain. Somatic cells treated with 5'-azacytidine also sho...

journal_title：Experimental cell research

authors： Mitchell AR

更新日期：1992-09-01 00:00:00

abstract：:Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in the early stage of pancreatic cancer, contributing to the poor prognosis. Accordingly, in this study, we evaluated the molecular mechanisms underlying invasion and metastasis. Using mass spectrometry, we found that Integrin alp...

journal_title：Experimental cell research

authors： Wu Y,Tan X,Liu P,Yang Y,Huang Y,Liu X,Meng X,Yu B,Wu M,Jin H

更新日期：2019-06-01 00:00:00

abstract：:Growth and survival of tumors at a site of metastasis involve interactions with stromal cells in the surrounding environment. Stromal cells aid tumor cell growth by producing cytokines as well as by modifying the environment surrounding the tumor through modulation of the extracellular matrix (ECM). Small leucine-rich...

journal_title：Experimental cell research

authors： Coulson-Thomas VJ,Gesteira TF,Coulson-Thomas YM,Vicente CM,Tersariol IL,Nader HB,Toma L

更新日期：2010-11-15 00:00:00

abstract：:Alternative splicing is an important mechanism that can disrupt cell cycle control resulting in tumorigenesis. Although many alterations of Transforming Growth Factor Beta (TGFβ) signaling are reported in cancers, the role of splice aberrations in destabilizing this signaling is the least understood mechanism. In this...

journal_title：Experimental cell research

authors： Sivadas VP,Gulati S,Varghese BT,Balan A,Kannan S

更新日期：2014-09-10 00:00:00

abstract：:The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...

journal_title：Experimental cell research

authors： Cui L,Li M,Feng F,Yang Y,Hang X,Cui J,Gao J

更新日期：2014-10-15 00:00:00

abstract：:For cancer metastasis, tumor cells present in the circulation must first adhere to the endothelium. Integrins play a central role in leukocyte adhesion to the endothelium and subsequent migration into tissues. The majority of tumor cells derived from solid cancers, including breast cancer, do not express integrins. We...

journal_title：Experimental cell research

authors： Mine S,Fujisaki T,Kawahara C,Tabata T,Iida T,Yasuda M,Yoneda T,Tanaka Y

更新日期：2003-08-01 00:00:00

abstract：:In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...

journal_title：Experimental cell research

authors： Johnson-Pais TL,Leach RJ

更新日期：1996-07-10 00:00:00

abstract：:Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

journal_title：Experimental cell research

authors： Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

更新日期：1998-07-10 00:00:00

abstract：:Fluorescence photobleaching recovery has been employed to study the lateral mobility of the Sendai virus envelope glycoproteins (HN, neuraminidase/hemagglutinin protein (HN) fusion protein (F) on the surface of human erythrocytes. Our results indicate that the two viral glycoproteins are laterally immobile on the cell...

journal_title：Experimental cell research

authors： Henis YI,Gutman O,Loyter A

更新日期：1985-10-01 00:00:00

abstract：:In preceding studies, the IP3-sensitive Ca2+ store of the hamster insulinoma cell line, HIT, was detected in cell surface protrusions such as microvilli and related membrane structures [Lange, K., and Brandt, U. (1993) FEBS Lett. 320, 183-188; and (1993) FEBS Lett. 325, 205-209]. In this study, these experiments were ...

journal_title：Experimental cell research

authors： Lange J,Schlieps K,Lange K,Brandt U,Knoll-Köhler E

更新日期：1996-11-01 00:00:00

abstract：:The release of cell surface-derived microparticles, or ectosomes, has now been described for many different cell types. In various diseases characterized by systemic inflammation, the numbers of ectosomes released from specific cell-types are found increased manifold in the circulation. Their pro-inflammatory and pro-...

journal_title：Experimental cell research

authors： Gasser O,Schifferli JA

更新日期：2005-07-15 00:00:00

abstract：:Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respective...

journal_title：Experimental cell research

authors： Keenan J,Joyce H,Aherne S,O'Dea S,Doolan P,Lynch V,Clynes M

更新日期：2012-03-10 00:00:00

abstract：:Autophagy has been shown to be critically associated with the central mechanisms underlying Parkinson's disease (PD), while the mechanisms contributing to the imbalance of autophagy remain unclear. Small nucleolar RNA host gene 1 (SNHG1), a well-studied long noncoding RNA, has been reported to be significantly increas...

journal_title：Experimental cell research

authors： Qian C,Ye Y,Mao H,Yao L,Sun X,Wang B,Zhang H,Xie L,Zhang H,Zhang Y,Zhang S,He X

更新日期：2019-11-01 00:00:00

abstract：:Elongation factor-1 alpha (EF-1 alpha) is a highly conserved, abundantly expressed protein that functions in peptide elongation during mRNA translation. Mammalian species contain a second EF-1 alpha gene, S1, whose expression is limited to brain, heart, and skeletal muscle. Such tissue specificity in S1 gene expressio...

journal_title：Experimental cell research

authors： Lee S,LeBlanc A,Duttaroy A,Wang E

更新日期：1995-08-01 00:00:00

abstract：:Recent studies have demonstrated a central role for the exchange protein activated by cAMP (Epac) in the inhibition of Fcgamma-receptor-mediated phagocytosis and bacterial killing by prostaglandin E(2) (PGE(2)) in macrophages. However, the subcellular localization of Epac, and its primary target Rap1, has yet to be de...

journal_title：Experimental cell research

authors： Brock TG,Serezani CH,Carstens JK,Peters-Golden M,Aronoff DM

更新日期：2008-01-15 00:00:00

abstract：:Serelaxin, a recombinant form of human relaxin-2, is currently regarded as a novel drug for treatment of acute heart failure. However, whether therapeutic effects of serelaxin are achieved by inhibiting cardiac fibrosis remains unclear. In this study, we investigate effects of serelaxin on inhibiting cardiac fibrosis....

journal_title：Experimental cell research

authors： Wu XP,Wang HJ,Wang YL,Shen HR,Tan YZ

更新日期：2018-01-01 00:00:00

abstract：:Sperm cells from control donors of proven fertility and men from barren couples were studied by conventional procedures, i.e., light microscopy as well as flow cytometry. Light microscopy analysis of semen included the measurement of spermatozoa concentration, morphology, and motility. All the men from barren couples ...

journal_title：Experimental cell research

authors： Troiano L,Granata AR,Cossarizza A,Kalashnikova G,Bianchi R,Pini G,Tropea F,Carani C,Franceschi C

更新日期：1998-06-15 00:00:00

abstract：:The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ioni...

journal_title：Experimental cell research

authors： Bouvier D,Hubert J,Seve AP,Bouteille M

更新日期：1985-02-01 00:00:00