Abstract：
:Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma membrane. We observed that Arf6 also affects a second network of very long and stable tubules containing MHC-I, many of which correspond to deep invaginations of the plasma membrane. Treatment with wortmannin, an inhibitor of phosphatidylinositol-3-phosphate kinase, prevents formation of the short dynamic tubules while increasing the number of the long and very stable ones. Expression of NefAAAA, a mutant form of HIV Nef, increases the number of cells containing the stable tubules, and is used here as a tool to facilitate their study. Photoactivation of NefAAAA-PA-GFP demonstrates that this molecule traffics from endosomes to the tubules. Finally, live-cell imaging also shows internalization of MHC-I molecules into these tubules, suggesting that this is an additional route for MHC-I traffic.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Massol RH,Larsen JE,Kirchhausen Tdoi
10.1016/j.yexcr.2005.01.021keywords:
subject
Has Abstractpub_date
2005-05-15 00:00:00pages
142-9issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(05)00044-3journal_volume
306pub_type
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