Sendai virus envelope glycoproteins become laterally mobile on the surface of human erythrocytes following fusion.

abstract：:Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-rela...

journal_title：Experimental cell research

authors： Sano M,Umezawa A,Abe H,Akatsuka A,Nonaka S,Shimizu H,Fukuma M,Hata J

更新日期：2001-05-15 00:00:00

abstract：:Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodi...

journal_title：Experimental cell research

authors： Fayein NA,Courtois Y,Jeanny JC

更新日期：1990-05-01 00:00:00

abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

journal_title：Experimental cell research

authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

更新日期：2012-03-10 00:00:00

abstract：:Parietal yolk sac cells M1536 -B3 grown on cytodex 2 beads deposited an extracellular matrix on the surface of the beads. Cell-free matrix-coated beads were isolated by treatment of the cell monolayer with cytochalasin B (CB) at a concentration of 10 micrograms/ml of phosphate-buffered saline (PBS). The matrix when an...

journal_title：Experimental cell research

authors： Mai S,Chung AE

更新日期：1984-06-01 00:00:00

abstract：:A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...

journal_title：Experimental cell research

authors： Cavadas MAS,Cheong A,Taylor CT

更新日期：2017-07-15 00:00:00

abstract：:The glomerular capillary endothelium is highly specialized to support the selective filtration of massive volumes of plasma. Filtration is driven by Starling forces acting across the glomerular capillary wall, and depends on its large surface area and extremely high water permeability. Glomerular endothelial cells are...

journal_title：Experimental cell research

authors： Obeidat M,Obeidat M,Ballermann BJ

更新日期：2012-05-15 00:00:00

abstract：:Although 12-day-old chick embryo retinal pigment epithelial (RPE) cells in situ do not express the proliferating cell nuclear antigen (PCNA) which is known to function as an auxiliary protein of DNA polymerase delta, they do so when cultured on glass. Conversely, PCNA was not expressed by RPE cells of the same age mai...

journal_title：Experimental cell research

authors： Hergott GJ,Kalnins VI

更新日期：1991-08-01 00:00:00

abstract：:The effect of the cyclin-dependent (CDK) inhibitors olomoucine and roscovitine on cell kinetics was studied. To this end, nonsmall cell lung cancer (NSCLC) cell line MR65 and neuroblastoma cell line CHP-212 were pulse labeled with bromodeoxyuridine (BrdUrd) and chased in culture medium, to which various concentrations...

journal_title：Experimental cell research

authors： Schutte B,Nieland L,van Engeland M,Henfling ME,Meijer L,Ramaekers FC

更新日期：1997-10-10 00:00:00

abstract：:We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-...

journal_title：Experimental cell research

authors： Kim H,You S,Kim IJ,Farris J,Foster LK,Foster DN

更新日期：2001-05-01 00:00:00

abstract：:Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, ...

journal_title：Experimental cell research

authors： Clawson GA,Friend DS,Smuckler EA

更新日期：1984-11-01 00:00:00

abstract：:High glucose concentrations associated with diabetes have been shown to cause the nonenzymatic modification of proteins. Reducing sugars covalently bind to free amine groups, undergo Amadori rearrangements, and crosslink with other glucose-modified proteins. Crosslinking of type I collagen by incubation with different...

journal_title：Experimental cell research

authors： Howard EW,Benton R,Ahern-Moore J,Tomasek JJ

更新日期：1996-10-10 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is one of the most common cancers diagnosed worldwide. However, the mechanism underlying HCC pathogenesis remains unknown. In the present study, TRIM24 was found increased in human HCC clinical samples and positively correlated with HCC tumor grade. Furthermore, TRIM24 knockdown inhibits...

journal_title：Experimental cell research

authors： Zhu Y,Zhao L,Shi K,Huang Z,Chen B

更新日期：2018-06-15 00:00:00

abstract：:The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...

journal_title：Experimental cell research

authors： Broadley C,Dawidowicz E,Chong PL,Hoover R

更新日期：1991-03-01 00:00:00

abstract：:The release of cell surface-derived microparticles, or ectosomes, has now been described for many different cell types. In various diseases characterized by systemic inflammation, the numbers of ectosomes released from specific cell-types are found increased manifold in the circulation. Their pro-inflammatory and pro-...

journal_title：Experimental cell research

authors： Gasser O,Schifferli JA

更新日期：2005-07-15 00:00:00

abstract：:In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...

journal_title：Experimental cell research

authors： Valmassoi J,Schwarz RI

更新日期：1988-06-01 00:00:00

abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

journal_title：Experimental cell research

authors： Zhang J,Harrison JS,Studzinski GP

更新日期：2011-01-01 00:00:00

abstract：:During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...

journal_title：Experimental cell research

authors： Krause S,Fakan S,Weis K,Wahle E

更新日期：1994-09-01 00:00:00

abstract：:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...

journal_title：Experimental cell research

authors： Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi V

更新日期：1999-12-15 00:00:00

abstract：:It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...

journal_title：Experimental cell research

authors： Chang H,Wang Y,Wu Y,Ma P,Song Y,Liu C,Ye Y,Qi JH,Qi Z

更新日期：2020-10-01 00:00:00

abstract：:Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

journal_title：Experimental cell research

authors： De Wever B,Rooman RP,De Brabander M

更新日期：1990-01-01 00:00:00

abstract：:An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...

journal_title：Experimental cell research

authors： Khan S,Collins RJ,Dunn IS

更新日期：1996-02-25 00:00:00

abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...

journal_title：Experimental cell research

authors： Chang C,Takayanagi A,Yoshida T,Shimizu N

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

journal_title：Experimental cell research

authors： Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

更新日期：2019-10-01 00:00:00

abstract：:Ezrin-Radixin-Moesin (ERM) binding phosphoprotein 50 (EBP50, a.k.a. NHERF-1) is a scaffold protein essential for the localization and coordinated activity of apical transporters, enzymes and receptors in epithelial cells. EBP50 acts via multiple protein binding interactions, including oligomerization through interacti...

journal_title：Experimental cell research

authors： Fouassier L,Nichols MT,Gidey E,McWilliams RR,Robin H,Finnigan C,Howell KE,Housset C,Doctor RB

更新日期：2005-05-15 00:00:00

abstract：:We have studied the internalization of targeted fusogenic liposome content to leukemic T cells (CEM) in vitro. We describe a method for the covalent coupling of T101 antibody to the surface of liposomes and the incorporation of fusogenic viral protein into the liposome membrane. Hygromycin B, an impermeant inhibitor o...

journal_title：Experimental cell research

authors： Sechoy O,Vidal M,Philippot JR,Bienvenue A

更新日期：1989-11-01 00:00:00

abstract：:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1...

journal_title：Experimental cell research

authors： Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal L

更新日期：2004-07-01 00:00:00

abstract：:In preparation for studies on the growth factor requirements of normal and transformed human fibroblasts, we have developed a serum-free medium that supports vigorous long-term serial subculture of diploid human fibroblasts and allows them to form large-sized colonies with high efficiency (40 to 60%) when plated at cl...

journal_title：Experimental cell research

authors： Ryan PA,Maher VM,McCormick JJ

更新日期：1987-10-01 00:00:00

abstract：:The formation of capillaries during development and tissue repair is likely to involve active reorganization of the actin cytoskeleton, although few studies have addressed this issue. Here, we have utilized an in vitro model of capillary morphogenesis whereby human umbilical vein endothelial cells are suspended within...

journal_title：Experimental cell research

authors： Salazar R,Bell SE,Davis GE

更新日期：1999-05-25 00:00:00

abstract：:Focal Adhesion Kinase (FAK) is a non-receptor tyrosine kinase that plays a key role in cellular processes such as cell adhesion, migration, proliferation and survival. Recent studies have also implicated FAK in the regulation of cell-cell adhesion. Here, evidence is presented showing that siRNA-mediated suppression of...

journal_title：Experimental cell research

authors： Playford MP,Vadali K,Cai X,Burridge K,Schaller MD

更新日期：2008-10-15 00:00:00

abstract：:There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

journal_title：Experimental cell research

authors： D'Silva I,Poirier GG,Heath MC

更新日期：1998-12-15 00:00:00