Abstract:
:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1) and actin from the nuclei of sparse cultures. We show that ZO-2 presents several NLS at its amino region, that when deleted, diminish the nuclear import of the ZO-2 amino segment and impair the ability of the region to regulate the transcriptional activity of promoters controlled by AP-1. Several RS repeats are detected in the ZO-2 amino segment, however, their deletion does not preclude the display of a speckled nuclear pattern. ZO-2 displays two putative NES. However, only the second one appears to be functional, as when conjugated to ovalbumin (OV), it is able to translocate this protein from the nucleus to the cytoplasm in a leptomycin B-sensitive way.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal Ldoi
10.1016/j.yexcr.2004.03.021keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
247-58issue
1eissn
0014-4827issn
1090-2422pii
S0014482704001181journal_volume
297pub_type
杂志文章abstract::In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90034-7
更新日期:1989-11-01 00:00:00
abstract::Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2020.111925
更新日期:2020-04-15 00:00:00
abstract::Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.09.031
更新日期:2015-01-01 00:00:00
abstract::Dynamic subcellular localization is an important regulatory mechanism for many proteins. cIAP1 and cIAP2 are two closely related members of inhibitor of apoptosis (IAP) family that play a role both as caspase inhibitors and as mediators of tumor necrosis factor (TNF) receptor signaling. Here, we report that cIAP1 and ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.04.040
更新日期:2004-08-15 00:00:00
abstract::An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90188-0
更新日期:1985-10-01 00:00:00
abstract::A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.12.002
更新日期:2008-03-10 00:00:00
abstract::Gicerin is a novel cell adhesion molecule in the immunoglobulin superfamily and has both homophilic adhesion and heterophilic adhesive activity to neurite outgrowth factor (NOF), an extracellular matrix protein in the laminin family. We investigated the possible involvement of gicerin in oviductal development, regener...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4367
更新日期:1999-03-15 00:00:00
abstract::Skin fibroblasts from patients with inherited adenomatosis of the large bowel (ACR-SF) possess alterations in actin microfilament (MF) organization which serve to distinguish "predisposed" cells from fibroblasts derived from normal individuals (NSF). MF bundle frequency and diameter were considerably reduced in ACR-SF...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90389-c
更新日期:1991-08-01 00:00:00
abstract::L1 cell adhesion molecule (L1-CAM) is a transmembrane cell adhesion molecule initially defined as a promigratory molecule in the developing nervous system. L1 is also overexpressed in a variety of human carcinomas and is associated with bad prognosis. In carcinoma cell lines L1 augments cell motility and metastasis, t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.04.004
更新日期:2008-08-01 00:00:00
abstract::Secretory phospholipase A(2) (sPLA(2)) plays important roles in mediating various cellular processes, including cell proliferation, differentiation, apoptosis, and inflammatory response. In this study, we demonstrated that a basic sPLA(2) inhibits epidermal growth factor (EGF)-induced EGF receptor activation, as deter...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5622
更新日期:2002-10-01 00:00:00
abstract::The liver plays critical roles in both homeostasis and pathology. It is the major site of drug metabolism in the body and, as such, a common target for drug-induced toxicity and is susceptible to a wide range of diseases. In contrast to other solid organs, the liver possesses the unique ability to regenerate. The phys...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2017.12.023
更新日期:2018-02-01 00:00:00
abstract::Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90211-r
更新日期:1990-01-01 00:00:00
abstract::The chemokine macrophage inflammatory protein-1 alpha [MIP-1alpha] causes migration of B cells and also induces changes in antibody secretion. However, the signal transduction pathways leading to these phenotypic changes remain undefined. We have identified a signal transduction pathway initiated by MIP-1alpha in B ce...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5272
更新日期:2001-08-01 00:00:00
abstract::Human neutrophils developed long thin tubulovesicular extensions (cytonemes) upon adhesion to fibronectin-coated substrata, when spreading was blocked. We observed extension formation when neutrophils were plated to fibronectin-coated substrata in Na(+)-free extracellular medium or in the presence of drugs capable of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5227
更新日期:2001-06-10 00:00:00
abstract::A disintegrin and metalloproteinase 33 (ADAM33) has been identified as a susceptibility gene for asthma, but details of the causality are not fully understood. We hypothesize that soluble ADAM33 (sADAM33) overexpression can alter the mechanical behaviors of airway smooth muscle cells (ASMCs) via regulation of the cell...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.10.004
更新日期:2016-11-15 00:00:00
abstract::Local regulation of the cortical cytoskeleton controls cell surface dynamics. GAP-43 and MARCKS are two abundant cytosolic protein kinase C substrates that are anchored to the cell membrane via acyl groups and interact with the cortical cytoskeleton. Each of them has been implicated in several forms of motility involv...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3709
更新日期:1997-10-10 00:00:00
abstract::Tetracyclines have long been used as valuable broad-spectrum antibiotics. The high antibacterial activity of tetracyclines, combined with their good tolerability, has led to their widespread use in treating various infectious diseases. However, similar to other antibiotics, tetracyclines are also known for their adver...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.11.012
更新日期:2014-01-15 00:00:00
abstract::Annulate lamellae (AL) are cytoplasmic arrays of stacked membrane cisternae containing densely packed pore complexes which are similar in structure to the nuclear pore complexes (NPCs) and thus referred to as annulate lamella pore complexes (ALPCs). We have recently shown that the integral nuclear pore membrane protei...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4935
更新日期:2000-08-25 00:00:00
abstract::In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90583-5
更新日期:1986-08-01 00:00:00
abstract::Using the restriction enzymes MspI and HpaII in the nick translation procedure it has been shown that decondensation of the paracentric heterochromatin of chromosome 9 during human spermatogenesis is associated with hypomethylation of the DNA sequences in this domain. Somatic cells treated with 5'-azacytidine also sho...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90421-4
更新日期:1992-09-01 00:00:00
abstract::SIRT6, with both deacetylase and ADP-ribosyltransferase activities, is predominantly expressed in the nucleus. It has been revealed that SIRT6 regulates various biological functions including metabolism, aging and stress resistance. This study aims to investigate the role of SIRT6 in vascular inflammation and it molec...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.05.001
更新日期:2017-08-01 00:00:00
abstract::The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines. The three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. Bromodeoxyuridine becomes incorporated into the nuclear DNA and increases the spon...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(78)90374-9
更新日期:1978-05-01 00:00:00
abstract::Rat IPC-81 promyelocytic leukemia cells responded to cAMP analog by undergoing apoptotic cell death both when anchored to fibronectin and when free in the medium. The protein kinase C stimulator 12-O-tetradecanoylphorbol 13-acetate enhanced the anchoring to substratum without impeding cAMP-induced cell death. The immo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1132
更新日期:1993-05-01 00:00:00
abstract::The adenine nucleotide translocator-2 (ANT2) gene is expressed in growth-activated cells together with the early-immediate genes. We have studied the mechanism of ANT2 expression during the serum-induced transition from G0 to G1 and during reentry into G0 as cells approach confluence. Actinomycin D completely blocked ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4432
更新日期:1999-05-01 00:00:00
abstract::The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90351-t
更新日期:1991-06-01 00:00:00
abstract::Recently, SIRT5 was reported to be a predominant desuccinylase and demalonylase in mitochondria. Ablation of SIRT5 enhances the systemic succinylation and malonylation of mitochondrial proteins, including various metabolic enzymes; however, its function in pancreatic β cells has not yet been clarified. In this study, ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.08.011
更新日期:2018-10-01 00:00:00
abstract::Bovine papilloma virus-transformed hamster embryo fibroblasts (HEF-BPV) reacted to exposure to tricyclodecan-9-yl-xanthogenate (D609) with immediate reversion to the growth kinetics and the flat morphology of the untransformed parental cells. After six population doublings in the presence of D609, clones which display...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90109-0
更新日期:1985-12-01 00:00:00
abstract::The effect of 1 microM antimycin on the proliferative properties, metabolism and basic cell composition of Ehrlich ascites tumour cells cultured in the second in vitro passage was studied. Continuous drug exposure of asynchronous cells caused rapid cessation of cell growth, characterized by the cell number and DNA, RN...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90162-4
更新日期:1985-03-01 00:00:00
abstract::A two-site radio-immunoassay for beta NGF demonstrated 5-10 pg of NGF in the normal, adult rat iris. Ciliarectomy or sympathectomy did not significantly alter the amount of NGF after 10 days. However, denervation including all sensory axons (stereotactic lesion distal to the trigeminal ganglion) increased the level to...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90155-6
更新日期:1983-10-15 00:00:00
abstract::Mesenchymal stem cells (MSCs) are non-immunogenic, multipotent cells with at least trilineage differentiation potential. They promote wound healing, improve regeneration of injured tissue, and mediate numerous other health effects. MSCs migrate to sites of injury and stimulate repair either through direct differentiat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.08.019
更新日期:2015-10-15 00:00:00