Characterization of the tight junction protein ZO-2 localized at the nucleus of epithelial cells.


:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1) and actin from the nuclei of sparse cultures. We show that ZO-2 presents several NLS at its amino region, that when deleted, diminish the nuclear import of the ZO-2 amino segment and impair the ability of the region to regulate the transcriptional activity of promoters controlled by AP-1. Several RS repeats are detected in the ZO-2 amino segment, however, their deletion does not preclude the display of a speckled nuclear pattern. ZO-2 displays two putative NES. However, only the second one appears to be functional, as when conjugated to ovalbumin (OV), it is able to translocate this protein from the nucleus to the cytoplasm in a leptomycin B-sensitive way.


Exp Cell Res


Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal L





Has Abstract


2004-07-01 00:00:00














  • Cell cycle-dependent DNA damage signaling induced by ICRF-193 involves ATM, ATR, CHK2, and BRCA1.

    abstract::Topoisomerase II is essential for cell proliferation and survival and has been a target of various anticancer drugs. ICRF-193 has long been used as a catalytic inhibitor to study the function of topoisomerase II. Here, we show that ICRF-193 treatment induces DNA damage signaling. Treatment with ICRF-193 induced G2 arr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Park I,Avraham HK

    更新日期:2006-07-01 00:00:00

  • Target proteins for arginine-specific mono(ADP-ribosyl) transferase in membrane fractions from chick skeletal muscle cells.

    abstract::In a previous study, we found that a specific inhibitor of cellular arginine-specific mono(ADP-ribosyl) transferase, meta-iodobenzylguanidine (MIBG), reversibly inhibited both proliferation and differentiation of cultured embryonic chick primary muscle myoblasts. In addition, we observed that arginine-specific ADP-rib...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Huang HY,Zhou H,Huiatt TW,Graves DJ

    更新日期:1996-07-10 00:00:00

  • p66Shc promotes HCC progression in the tumor microenvironment via STAT3 signaling.

    abstract::The development of hepatocellular carcinoma (HCC) is strongly associated with chronic inflammation. p66Shc is an oxidase previously shown to promote androgen-independent cell growth through generation of reactive oxygen species. However, the importance and biologic functions of p66Shc in HCC are unclear. The clinical ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Huang P,Feng X,Zhao Z,Yang B,Fang T,Guo M,Xia J

    更新日期:2019-10-15 00:00:00

  • SV40 large T antigen reinduces the cell cycle in terminally differentiated myotubes through inducing Cdk2, Cdc2, and their partner cyclins.

    abstract::Terminally differentiated skeletal muscle myotubes are arrested in G0 phase of the cell cycle and are unable to be released from this arrest by stimulation with mitogens including serum and growth factors. To inspect a possibility of reversing the quiescence at the G0 phase, we have exploited the mouse skeletal muscle...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ohkubo Y,Kishimoto T,Nakata T,Yasuda H,Endo T

    更新日期:1994-09-01 00:00:00

  • The timing of initiation of macronuclear DNA synthesis is set during the preceding cell cycle in Paramecium tetraurelia. Analysis of the effects of abrupt changes in nutrient level.

    abstract::In many eukaryotic organisms, initiation of DNA synthesis is associated with a major control point within the cell cycle and reflects the commitment of the cell to the DNA replication-division portion of the cell cycle. In Paramecium, the timing of DNA synthesis initiation is established prior to fission during the pr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ching AS,Berger JD

    更新日期:1986-11-01 00:00:00

  • DNA fiber replication during a morphogenetic switch in the shoot meristematic cells of a higher plant.

    abstract::Chromosomal DNA fiber replication was investigated in the shoot meristem of mustard plants during the morphogenetic transition from the leaf-forming (vegetative) to the flower-forming (evoked) condition. The replicon size, determined using the modal class, was 15 micron in the vegetative meristem and shifted to 7.5 mi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jacqmard A,Houssa C

    更新日期:1988-12-01 00:00:00

  • Identification and molecular cloning of a functional GDP-fucose transporter in Drosophila melanogaster.

    abstract::Nucleotide sugar transporters play a central role in the process of glycosylation. They are responsible for the translocation of nucleotide sugars from the cytosol, their site of synthesis, into the Golgi apparatus where the activated sugars serve as substrates for a variety of glycosyltransferases. We and others have...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lühn K,Laskowska A,Pielage J,Klämbt C,Ipe U,Vestweber D,Wild MK

    更新日期:2004-12-10 00:00:00

  • Analysis of mtDNA copy number and composition of single mitochondrial particles using flow cytometry and PCR.

    abstract::Mitochondrial DNA (mtDNA) is a multicopy, maternally inherited, genome. Individuals frequently carry a mixture of genetically distinct mtDNA molecules whose proportions may vary between sexual generations or among tissues from the same individual. Analyses of the genetic composition of mitochondria have previously rel...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cavelier L,Johannisson A,Gyllensten U

    更新日期:2000-08-25 00:00:00

  • A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids.

    abstract::We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Keherly MJ,Papaconstantinou J,McCombs JL

    更新日期:1993-04-01 00:00:00

  • Tg737 acts as a key driver of invasion and migration in liver cancer stem cells and correlates with poor prognosis in patients with hepatocellular carcinoma.

    abstract::We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: You N,Tan Y,Zhou L,Huang X,Wang W,Wang L,Wu K,Mi N,Li J,Zheng L

    更新日期:2017-09-15 00:00:00

  • Tenogenic differentiation of mesenchymal stem cells and noncoding RNA: From bench to bedside.

    abstract::Tendon is a critical unit of musculoskeletal system that connects muscle to bone to control bone movement. More population participate in physical activities, tendon injuries, such as acute tendon rupture and tendinopathy due to overuse, are common causing unbearable pain and disability. However, the process of tendon...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Lu YF,Chan KM,Li G,Zhang JF

    更新日期:2016-02-15 00:00:00

  • Viscoelasticity in wild-type and vinculin-deficient (5.51) mouse F9 embryonic carcinoma cells examined by atomic force microscopy and rheology.

    abstract::We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wild-type cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wild-type and 5.51 cells by measuring the dynamic response of controlled lo...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Goldmann WH,Ezzell RM

    更新日期:1996-07-10 00:00:00

  • Immunofluorescent localization of histone H10 in the nuclei of proliferating and differentiating Friend cells.

    abstract::By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all ove...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Banchev T,Srebreva L,Zlatanova J,Tsanev R

    更新日期:1988-07-01 00:00:00

  • N-cadherin function is required for differentiation-dependent cytoskeletal reorganization in lens cells in vitro.

    abstract::Members of the cadherin family of cell adhesion molecules participate in calcium-dependent cell-cell adhesions that are necessary for the cell sorting events that regulate early developmental processes. Although individual cadherin molecules have been shown to participate in tissue histogenesis, the regulation of func...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ferreira-Cornwell MC,Veneziale RW,Grunwald GB,Menko AS

    更新日期:2000-04-10 00:00:00

  • Constitutively active BMP type I receptors transduce BMP-2 signals without the ligand in C2C12 myoblasts.

    abstract::Bone morphogenetic protein-2 (BMP-2), a member of transforming growth factor-beta superfamily, inhibits the terminal differentiation of C2C12 myoblasts and changes their differentiation pathway into cells expressing osteoblast phenotypes such as alkaline phosphatase (ALP) activity and osteocalcin production (Katagiri ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Akiyama S,Katagiri T,Namiki M,Yamaji N,Yamamoto N,Miyama K,Shibuya H,Ueno N,Wozney JM,Suda T

    更新日期:1997-09-15 00:00:00

  • Aggregation of sponge cells. XIV. Possible substitution of calcium ions by polycations.

    abstract::Single cells from the siliceous sponge Geodia cydonium, obtained after chemical dissociation, reaggregate in the presence of the aggregation factor in Ca2+-containing medium to large aggregates. It was found that polyvalent organic cations (polylysine, spermine, spermidine, putrescine) enhance the Ca2+-mediated cell a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Müller WE,Zahn RK,Kurelec B,Müller I

    更新日期:1978-05-01 00:00:00

  • FR901228, a potent antitumor antibiotic, is a novel histone deacetylase inhibitor.

    abstract::Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

    更新日期:1998-05-25 00:00:00

  • Estrogen receptor alpha (ESR1)-signaling regulates the expression of the taxane-response biomarker PRP4K.

    abstract::The pre-mRNA splicing factor 4 kinase PRP4K (PRPF4B), is an essential kinase that is a component of the U5 snRNP and functions in spliceosome assembly. We demonstrated that PRP4K is a novel biological marker for taxane response in ovarian cancer patients and reduced levels of PRP4K correlate with intrinsic and acquire...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lahsaee S,Corkery DP,Anthes LE,Holly A,Dellaire G

    更新日期:2016-01-01 00:00:00

  • The extracellular matrix in breast cancer predicts prognosis through composition, splicing, and crosslinking.

    abstract::The extracellular matrix in the healthy breast has an important tumor suppressive role, whereas the abnormal ECM in tumors can promote aggressiveness, and has been linked to breast cancer relapse, survival and resistance to chemotherapy. This review article gives an overview of the elements of the ECM which have been ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Robertson C

    更新日期:2016-04-10 00:00:00

  • Homeostatic regulation of meiotic DSB formation by ATM/ATR.

    abstract::Ataxia-telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Cooper TJ,Wardell K,Garcia V,Neale MJ

    更新日期:2014-11-15 00:00:00

  • NMNAT3 is involved in the protective effect of SIRT3 in Ang II-induced cardiac hypertrophy.

    abstract::Pathological cardiac hypertrophy is a maladaptive response in a variety of organic heart disease (OHD), which is characterized by mitochondrial dysfunction that results from disturbed energy metabolism. SIRT3, a mitochondria-localized sirtuin, regulates global mitochondrial lysine acetylation and preserves mitochondri...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yue Z,Ma Y,You J,Li Z,Ding Y,He P,Lu X,Jiang J,Chen S,Liu P

    更新日期:2016-10-01 00:00:00

  • Decreased tumorigenicity of c-Myc-transformed fibroblasts expressing active USF2.

    abstract::USF is a small family of basic helix-loop-helix leucine zipper (bHLH-zip) transcription factors with DNA binding specificities similar to that of the c-Myc oncoprotein. Evidence for a role of USF in growth control includes inhibition of c-Myc-dependent cellular transformation in vitro and loss of USF transcriptional a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Choe C,Chen N,Sawadogo M

    更新日期:2005-01-01 00:00:00

  • Long-term cultivation of amphibian melanophores. In vitro ageing and spontaneous transformation to a continuous cell line.

    abstract::Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in gr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kondo H,Ide H

    更新日期:1983-11-01 00:00:00

  • Cortactin involvement in the keratinocyte growth factor and fibroblast growth factor 10 promotion of migration and cortical actin assembly in human keratinocytes.

    abstract::Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ceccarelli S,Cardinali G,Aspite N,Picardo M,Marchese C,Torrisi MR,Mancini P

    更新日期:2007-05-15 00:00:00

  • Modulation of membrane cholesterol levels: effects on endothelial cell function.

    abstract::The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Broadley C,Dawidowicz E,Chong PL,Hoover R

    更新日期:1991-03-01 00:00:00

  • The intracellular domain of Jagged-1 interacts with Notch1 intracellular domain and promotes its degradation through Fbw7 E3 ligase.

    abstract::Notch signaling involves the proteolytic cleavage of the transmembrane Notch receptor after binding to its transmembrane ligands. Jagged-1 also undergoes proteolytic cleavage by gamma-secretase and releases an intracellular fragment. In this study, we have demonstrated that the Jagged-1 intracellular domain (JICD) inh...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kim MY,Jung J,Mo JS,Ann EJ,Ahn JS,Yoon JH,Park HS

    更新日期:2011-10-15 00:00:00

  • An antibody against 100- to 116-kDa polypeptides in coated vesicles inhibits triskelion binding.

    abstract::There is considerable evidence that the 100- to 116-kDa polypeptides in calf brain coated vesicles are involved in the assembly of clathrin triskelions to form coated vesicles. We have raised polyclonal antibodies against these polypeptides. By Western blot analysis, these antibodies bind to a distinct subset of the s...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mosley ST,Branton D

    更新日期:1988-02-01 00:00:00

  • Intracellular distribution of poly(ADP-ribose) synthetase in rat spermatogenic cells.

    abstract::The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemic...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Concha II,Figueroa J,Concha MI,Ueda K,Burzio LO

    更新日期:1989-02-01 00:00:00

  • Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells.

    abstract::Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Deslex S,Negrel R,Ailhaud G

    更新日期:1987-01-01 00:00:00

  • Regulation of MYPT1 stability by the E3 ubiquitin ligase SIAH2.

    abstract::Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Twomey E,Li Y,Lei J,Sodja C,Ribecco-Lutkiewicz M,Smith B,Fang H,Bani-Yaghoub M,McKinnell I,Sikorska M

    更新日期:2010-01-01 00:00:00