Migrational changes of mesenchymal stem cells in response to cytokines, growth factors, hypoxia, and aging.

abstract：:The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

journal_title：Experimental cell research

authors： Olsson AK,Nånberg E

更新日期：2001-04-15 00:00:00

abstract：:Gicerin is a novel cell adhesion molecule in the immunoglobulin superfamily and has both homophilic adhesion and heterophilic adhesive activity to neurite outgrowth factor (NOF), an extracellular matrix protein in the laminin family. We investigated the possible involvement of gicerin in oviductal development, regener...

journal_title：Experimental cell research

authors： Tsukamoto Y,Taira E,Kajimura K,Yamate J,Kotani T,Amin H,Kohama K,Sakuma S,Miki N,Sasaki F

更新日期：1999-03-15 00:00:00

abstract：:Müller cells are the predominant retinal glial cells. One of the key roles of Müller cells is in the uptake of the neurotransmitter glutamate and in its conversion to glutamine. Müller cell dysfunction due to oxidative stress elicited by high glutamate concentrations can lead to toxicity, which promote the pathogenesi...

journal_title：Experimental cell research

authors： Cheng Z,Zhang T,Zheng J,Ding W,Wang Y,Li Y,Zhu L,Murray M,Zhou F

更新日期：2019-10-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) secrete paracrine factors that could be cytoprotective and serve roles in immunoregulation during tissue injury. Although MSCs express HIV receptors, and co-receptors, and are susceptible to HIV infection, whether HIV-1 may affect biological properties of MSCs needs more study. We evaluat...

journal_title：Experimental cell research

authors： Cheng K,Rai P,Lan X,Plagov A,Malhotra A,Gupta S,Singhal PC

更新日期：2013-08-15 00:00:00

abstract：:The ability of primary cultures of rat hepatocytes to remove cyclobutane pyrimidine dimers (CPDs) from DNA fragments containing the transcriptionally active albumin gene and the transcriptionally inactive embryonic myosin heavy chain (MHCemb) and H-ras fragments as well as the genome overall was measured. At all UV do...

journal_title：Experimental cell research

authors： Guo Z,Heydari A,Richardson A

更新日期：1998-11-25 00:00:00

abstract：:We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. Human fibroblasts immortalized with an inducible SV40 T-antigen become senescent following the de-induction of T-antigen. Plasmids expressing an alternative source of intact T-antigen restore prol...

journal_title：Experimental cell research

authors： Shay JW,Pereira-Smith OM,Wright WE

更新日期：1991-09-01 00:00:00

abstract：:Proteolysis of corneodesmosin, a 52- to 56-kDa basic protein located in the extracellular part of the modified desmosomes (corneodesmosomes) of human cornified epithelia, is thought to be a key event of desquamation. Three monoclonal antibodies specific for human corneodesmosin were used to search for the expression o...

journal_title：Experimental cell research

authors： Montézin M,Simon M,Guerrin M,Serre G

更新日期：1997-02-25 00:00:00

abstract：:Previous work has suggested that adhesive or cytoskeletal structures alter during epithelial transformation. The purpose of the present studies was to see whether such reorganizations were accompanied by consistent changes in the biophysical characteristics of cells. We used in vitro cell lines from rat liver and resp...

journal_title：Experimental cell research

authors： Heckman CA,Campbell AE,Wetzel B

更新日期：1987-03-01 00:00:00

abstract：:Bone morphogenetic protein (BMP), a member of the TGF-beta superfamily, is involved in development, morphogenesis, cell proliferation and apoptosis. Dysregulation of BMP signaling has been suggested in tumorigenesis. In an analysis of human colon normal mucosa and tumors at different stages by immunohistochemistry, we...

journal_title：Experimental cell research

authors： Deng H,Makizumi R,Ravikumar TS,Dong H,Yang W,Yang WL

更新日期：2007-03-10 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：:Mesenchymal stem cells are universally regarded across many fields of medicine, as one of the most promising cell types for use in cell-based therapies. Although not yet fully understood, the therapeutic effects of these cells are largely attributed to the trophic actions of growth factors and cytokines present in the...

journal_title：Experimental cell research

authors： Bara JJ,Turner S,Roberts S,Griffiths G,Benson R,Trivedi JM,Wright KT

更新日期：2015-04-10 00:00:00

abstract：:Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...

journal_title：Experimental cell research

authors： Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist TH

更新日期：1996-08-25 00:00:00

abstract：:It is known that freshly dissociated thyroid cell clusters form follicles in suspension culture. Thyroid epithelial cell lines, grown for many generations in vitro, fail to show colloid-containing lumina when cultured as monolayers. Several thyroid cell lines, some transformed, have been tested with respect to their a...

journal_title：Experimental cell research

authors： Nitsch L,Tacchetti C,Tramontano D,Ambesi-Impiombato FS

更新日期：1984-05-01 00:00:00

abstract：:Liver cells isolated from the adult rat livers under mild conditions were preincubated for 1 day with Williams medium E (WE) containing serum, dexamethasone and insulin, and then the cells (monolayered) were incubated for 2-3 days with WE (1 ml) containing only insulin to measure DNA synthesis and/or mitosis. DNA synt...

journal_title：Experimental cell research

authors： Koji T,Terayama H

更新日期：1984-12-01 00:00:00

abstract：:The MUC6 mucin has a critical protective function in the normal stomach, pancreas and duodenum and is aberrantly expressed during the progression of some gastrointestinal cancers. Our aim was to determine whether MUC6 contributes to the etiology or progression of pancreatic cancer and elucidate the molecular basis of ...

journal_title：Experimental cell research

authors： Leir SH,Harris A

更新日期：2011-10-15 00:00:00

abstract：:The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular basement membrane (GBM), a specialized extracellular matrix that lies between them. Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteog...

journal_title：Experimental cell research

authors： Miner JH

更新日期：2012-05-15 00:00:00

abstract：:Mitochondrial DNA (mtDNA) represents 0.15% of the total cell DNA (at least an order of magnitude less than in liver or heart) of rabbit articular chondrocytes. Besides the already well-documented low respiratory activity, chondrocyte differentiation thus involves a specific control of mitochondrial biogenesis. When tr...

journal_title：Experimental cell research

authors： Champagne AM,Benel L,Ronot X,Mignotte F,Adolphe M,Mounolou JC

更新日期：1987-08-01 00:00:00

abstract：:Previous studies showed nanosecond pulsed electric field (nsPEF) can ablate solid tumors including hepatocellular carcinoma (HCC) but its effect on cell membrane is not fully understood. We hypothesized nsPEF disrupt the microdomains on outer-cellular membrane with direct mechanical force and as a result the plasma me...

journal_title：Experimental cell research

authors： Yin S,Chen X,Xie H,Zhou L,Guo D,Xu Y,Wu L,Zheng S

更新日期：2016-08-15 00:00:00

abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

journal_title：Experimental cell research

authors： Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

更新日期：1985-05-01 00:00:00

abstract：:The liver plays critical roles in both homeostasis and pathology. It is the major site of drug metabolism in the body and, as such, a common target for drug-induced toxicity and is susceptible to a wide range of diseases. In contrast to other solid organs, the liver possesses the unique ability to regenerate. The phys...

journal_title：Experimental cell research

authors： Beckwitt CH,Clark AM,Wheeler S,Taylor DL,Stolz DB,Griffith L,Wells A

更新日期：2018-02-01 00:00:00

abstract：:Hypoxic blood-brain barrier (BBB) dysfunction is a common feature of CNS diseases however mechanisms underlying barrier disturbance are still largely unknown. This study investigated the role of transforming growth factor β (TGFβ), a cytokine known to induce expression of the proprotein convertase Furin, in hypoxia-me...

journal_title：Experimental cell research

authors： Baumann J,Huang SF,Gassmann M,Tsao CC,Ogunshola OO

更新日期：2019-10-15 00:00:00

abstract：:Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is...

journal_title：Experimental cell research

authors： Ceccarelli S,Cardinali G,Aspite N,Picardo M,Marchese C,Torrisi MR,Mancini P

更新日期：2007-05-15 00:00:00

abstract：:It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...

journal_title：Experimental cell research

authors： Tabellini G,Bortul R,Santi S,Riccio M,Baldini G,Cappellini A,Billi AM,Berezney R,Ruggeri A,Cocco L,Martelli AM

更新日期：2003-07-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) support life-long spermatogenesis by self-renewing and producing spermatogonia committed to differentiation. In vitro, SSCs form three-dimensional spermatogonial aggregates (clusters) when cultured with glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (...

journal_title：Experimental cell research

authors： Ebata KT,Yeh JR,Zhang X,Nagano MC

更新日期：2011-06-10 00:00:00

abstract：:We developed an improved method for isolation of peroxisome biogenesis-defective somatic animal cell mutants, using a combination of green fluorescent protein (GFP) expression and the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) selection method. We used TKaG1 and TKaG2 cells, the wild-type Chinese hamster ovary (CHO) c...

journal_title：Experimental cell research

authors： Ghaedi K,Kawai A,Okumoto K,Tamura S,Shimozawa N,Suzuki Y,Kondo N,Fujiki Y

更新日期：1999-05-01 00:00:00

abstract：:Evidence is presented that endonuclease digestion of isolated, unfixed chromosomes results in the production of banding patterns similar to those produced by digestion of fixed, air-dried chromosomes. Mouse L cell chromosomes were isolated under acidic or relatively neutral pH conditions, exposed in situ (as wet mount...

journal_title：Experimental cell research

authors： Burkholder GD,Schmidt GJ

更新日期：1986-06-01 00:00:00

abstract：:ADP-ribosylation factor 3 (ARF3) is a member of the KRAS proto-oncogene, GTPase(Ras) super-family of guanine nucleotide-binding proteins that mediates Golgi-related mitosis, but its role in malignant cells is unclear. In the present study, we found that mRNA and protein expression of ARF3 is up-regulated in breast can...

journal_title：Experimental cell research

authors： Huang D,Pei Y,Dai C,Huang Y,Chen H,Chen X,Zhang X,Lin C,Wang H,Zhang R,Wan X,Wang L

更新日期：2019-11-15 00:00:00

abstract：:The enzyme activity hydrolysing diadenosine 5,5'-P1, P4-tetraphosphate (AP4A) was demonstrated in the embryonic extract of sea urchin. The enzyme activity was preferentially inhibited by ZnCl2 and by high concentrations of isobutylmethylxanthine, indicating that two types of the enzyme, (AP4A) hydrolase and non-specif...

journal_title：Experimental cell research

authors： Morioka M,Shimada H

更新日期：1987-03-01 00:00:00

abstract：:CUB domains are most exclusively found in secreted proteins and in a few transmembrane proteins. These domains are approximately 110 amino acids long and have four conserved cysteines that form a β-sandwich fold. CUB domains proteins are involved in a wide range of biological functions. We have shown that CUB domains ...

journal_title：Experimental cell research

authors： Predes D,Cruz JVR,Abreu JG,Mendes FA

更新日期：2019-10-01 00:00:00

abstract：:Various cell adhesion molecules mediate the diverse functions of the vascular endothelium, such as cell adhesion, neutrophil migration, and angiogenesis. In order to identify cell adhesion molecules important for angiogenesis, we used an in vitro model (Chalupowicz, Chowdhury, Bach, Barsigian, and Martinez, J. Cell Bi...

journal_title：Experimental cell research

authors： Bach TL,Barsigian C,Chalupowicz DG,Busler D,Yaen CH,Grant DS,Martinez J

更新日期：1998-02-01 00:00:00