当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > MIP-1alpha induces activation of phosphatidylinositol-3 kinase that associates with Pyk-2 and is necessary for B-cell migration.

MIP-1alpha induces activation of phosphatidylinositol-3 kinase that associates with Pyk-2 and is necessary for B-cell migration.

Abstract：

:The chemokine macrophage inflammatory protein-1 alpha [MIP-1alpha] causes migration of B cells and also induces changes in antibody secretion. However, the signal transduction pathways leading to these phenotypic changes remain undefined. We have identified a signal transduction pathway initiated by MIP-1alpha in B cells. Here we report that stimulation of tonsil B cells with MIP-1alpha induces phosphatidylinositol 3-kinase [PI3-K] activation. Kinase activity was transient with peak induction occurring within 2.5 to 5 min after stimulation and was dose-dependent. In addition stimulation with MIP-1alpha induces tyrosine phosphorylation of the proline-rich tyrosine kinase Pyk-2. Immunoprecipitation analysis showed a constitutive association between Pyk-2 and PI3-K and pretreatment of MIP-1alpha-stimulated B cells with wortmannin, a specific inhibitor of PI3-K, resulted in a loss of PI3-K activity. The PI3-K inhibitor wortmannin prevented B cells from migrating in response to MIP-1alpha. Hence, PI3-K and Pyk-2 seem to be components of a signal transduction pathway induced by stimulation of B cells with MIP-1alpha, and this pathway may play a role in B-cell migration.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Rumsey LM,Teague RM,Benedict SH,Chan MA

doi

10.1006/excr.2001.5272

keywords:

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

77-83

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(01)95272-3

journal_volume

268

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • MZF-1 and DbpA interact with DNase I hypersensitive sites that correlate with expression of the human MUC1 mucin gene.

    abstract：:The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.04.008

    authors： Shiraga T,Winpenny JP,Carter EJ,McCarthy VA,Hollingsworth MA,Harris A

    更新日期：2005-08-01 00:00:00

  • Increased mitochondrial-encoded gene transcription in immortal DF-1 cells.

    abstract：:We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5207

    authors： Kim H,You S,Kim IJ,Farris J,Foster LK,Foster DN

    更新日期：2001-05-01 00:00:00

  • Epigenetic specification of centromeres by CENP-A.

    abstract：:Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2009.07.023

    authors： Bernad R,Sánchez P,Losada A

    更新日期：2009-11-15 00:00:00

  • Phosphorylated 350 kD protein in the nucleus as it is associated with cell transformation.

    abstract：:Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90169-2

    authors： Sato C,Nishizawa K,Nakayama T,Hirai R,Nakamura H

    更新日期：1986-12-01 00:00:00

  • Reconstitution of dna synthetic capacity in senescent normal human fibroblasts by expressing cellular factors E2F and Mdm2.

    abstract：:Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenoviru...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5352

    authors： Sarraj S,Farb R,Martell RE

    更新日期：2001-11-01 00:00:00

  • Jun B expression is regulated differently by three mitogenic pathways in thyrocytes.

    abstract：:In dog thyrocytes in primary culture, thyrotropin (TSH), acting through cyclic AMP, induces proliferation and differentiation expression, while tetradecanoylphorbol acetate (TPA) or epidermal growth factor (EGF) induces proliferation and dedifferentiation. In this work, we have investigated the regulation of mRNA expr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1294

    authors： Pirson I,Dumont JE

    更新日期：1994-10-01 00:00:00

  • Cell-scaffold interaction within engineered tissue.

    abstract：:The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.02.028

    authors： Chen H,Liu Y,Jiang Z,Chen W,Yu Y,Hu Q

    更新日期：2014-05-01 00:00:00

  • Lithium inhibits terminal differentiation of erythroleukemia cells. Evidence for a pre-commitment 'priming' event.

    abstract：:Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90012-7

    authors： Zaricznyj C,Macara IG

    更新日期：1987-02-01 00:00:00

  • Immunoseparation and immunodetection of nucleic acids labeled with halogenated nucleotides.

    abstract：:A novel methodology for labeling, isolation, and detection of nucleic acids is described. Nucleic acid isolation is based on in vivo or in vitro incorporation of BrU or BrdU to either RNA or DNA, respectively, followed by immunoprecipitation of the labeled nucleic acid utilizing anti-BrdU MoAb, which crossreacts with ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3644

    authors： Haider SR,Juan G,Traganos F,Darzynkiewicz Z

    更新日期：1997-08-01 00:00:00

  • hnRNP M interacts with PSF and p54(nrb) and co-localizes within defined nuclear structures.

    abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.10.021

    authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

    更新日期：2010-02-01 00:00:00

  • Role of YAP/TAZ in cell-matrix adhesion-mediated signalling and mechanotransduction.

    abstract：:Signalling from the extracellular matrix (ECM) is a fundamental cellular input that sustains proliferation, opposes cell death and regulates differentiation. Through integrins, cells perceive both the chemical composition and physical properties of the ECM. In particular, cell behaviour is profoundly influenced by the...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2015.10.034

    authors： Dupont S

    更新日期：2016-04-10 00:00:00

  • Nucleocytoplasmic shuttling of STK16 (PKL12), a Golgi-resident serine/threonine kinase involved in VEGF expression regulation.

    abstract：:PKL12/STK16 protein is the first identified mammalian member of a ser/thr kinase subfamily that is conserved across several kingdoms, with a broad expression pattern in murine tissues and cell types. Endogenous STK16 subcellular localization was evaluated by indirect immunofluorescence in NIH/3T3 and NRK cells, demons...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.10.010

    authors： Guinea B,Ligos JM,Laín de Lera T,Martín-Caballero J,Flores J,Gonzalez de la Peña M,García-Castro J,Bernad A

    更新日期：2006-01-15 00:00:00

  • Complex formation between tissue transglutaminase II (tTG) and vascular endothelial growth factor receptor 2 (VEGFR-2): proposed mechanism for modulation of endothelial cell response to VEGF.

    abstract：:We have recently demonstrated that thrombin-activated FXIII (FXIIIA-subunit), a plasma transglutaminase, activates VEGFR-2 by crosslinking it with the alpha(v)beta(3) integrin on the surface of endothelial cells (EC), thereby stimulating angiogenesis. Tissue transglutaminase (tTG), which is functionally and structural...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.05.019

    authors： Dardik R,Inbal A

    更新日期：2006-10-01 00:00:00

  • Effects of antiandrogens on chromatin remodeling and transcription of the integrated mouse mammary tumor virus promoter.

    abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5018

    authors： List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

    更新日期：2000-10-10 00:00:00

  • Cell size of proliferating plant cells increases with temperature: implications in the control of cell division.

    abstract：:As chemical reactions related to the regulation of cell proliferation are governed by availability, amount, and concentration of relevant molecules, it has been suggested that cell size is an important factor in the control of cell cycle. We have measured the size of proliferating cells of Allium cepa roots in which g...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90056-6

    authors： Cuadrado A,Navarrete MH,Canovas JL

    更新日期：1989-11-01 00:00:00

  • Tfe3 expression is closely associated to macrophage terminal differentiation of human hematopoietic myeloid precursors.

    abstract：:The MItf-Tfe family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors encodes four family members: MItf, Tfe3, TfeB and TfeC. In vitro, each protein of the family binds DNA in a homo- or heterodimeric form with other family members. Tfe3 is involved in chromosomal translocations recurrent in di...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.09.015

    authors： Zanocco-Marani T,Vignudelli T,Gemelli C,Pirondi S,Testa A,Montanari M,Parenti S,Tenedini E,Grande A,Ferrari S

    更新日期：2006-12-10 00:00:00

  • Overexpression of ADAM9 enhances growth factor-mediated recycling of E-cadherin in human colon cancer cell line HT29 cells.

    abstract：:Growth factor-mediated stimulation of epithelial cells induces the disassembly of E-cadherin-mediated cell-cell adhesion. We found that overexpression of a disintegrin and metalloprotease 9 (ADAM9) enhanced growth factor-mediated induction of endocytosis and dynamic recycling of E-cadherin in HT29 human colon cancer c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.10.032

    authors： Hirao T,Nanba D,Tanaka M,Ishiguro H,Kinugasa Y,Doki Y,Yano M,Matsuura N,Monden M,Higashiyama S

    更新日期：2006-02-01 00:00:00

  • Ultrastructural studies on autolysosomes in rat hepatocytes after leupeptin treatment.

    abstract：:We have studied the morphological alterations of the lysosomal compartment in rat hepatocytes following intraperitoneal administration of leupeptin, using electron microscopy and cytochemical techniques. At 30 min after the injection, autophagic vacuoles (autophagosomes and autolysosomes), containing cytoplasmic organ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90436-6

    authors： Ishikawa T,Furuno K,Kato K

    更新日期：1983-03-01 00:00:00

  • Antiapoptotic effect of human T-cell leukemia virus type 1 tax protein correlates with its creb transcriptional activity.

    abstract：:Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.01.009

    authors： Trevisan R,Daprai L,Paloschi L,Vajente N,Chieco-Bianchi L,Saggioro D

    更新日期：2006-05-01 00:00:00

  • Drosophila mars is required for organizing kinetochore microtubules during mitosis.

    abstract：:Spindle assembly is essential for the equal distribution of genetic material to the daughter cells during mitosis. The process of spindle assembly is complicated and involves multiple levels of molecular regulation. It is generally accepted that mitotic spindles are emanated from the centrosomes and are assembled in t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.08.004

    authors： Yang CP,Fan SS

    更新日期：2008-10-15 00:00:00

  • Ex vivo cultures of glioblastoma in three-dimensional hydrogel maintain the original tumor growth behavior and are suitable for preclinical drug and radiation sensitivity screening.

    abstract：:Identification of new drugs and predicting drug response are major challenges in oncology, especially for brain tumors, because total surgical resection is difficult and radiation therapy or chemotherapy is often ineffective. With the aim of developing a culture system close to in vivo conditions for testing new drugs...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.12.010

    authors： Jiguet Jiglaire C,Baeza-Kallee N,Denicolaï E,Barets D,Metellus P,Padovani L,Chinot O,Figarella-Branger D,Fernandez C

    更新日期：2014-02-15 00:00:00

  • Do IL-3/GM-CSF effect on the myofibroblastic differentiation of human adipose derived stromal cells?

    abstract：BACKGROUND:Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast afte...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.03.056

    authors： Lee JS,Tae SS,Kim DY,Han SK,Kim WK,Dhong ES

    更新日期：2017-06-15 00:00:00

  • Long non-coding RNA HNF1A-AS1 upregulates OTX1 to enhance angiogenesis in colon cancer via the binding of transcription factor PBX3.

    abstract：:Colon cancer shows characteristics of metastasis, which is associated with angiogenesis. Increasing evidence highlights long non-coding RNAs (lncRNAs) as important participants in angiogenesis of cancers, including colon cancer. Hence, this study investigated the role of HNF1A-AS1 in angiogenesis of colon cancer. RT-q...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112025

    authors： Wu J,Meng X,Jia Y,Chai J,Wang J,Xue X,Dang T

    更新日期：2020-08-15 00:00:00

  • Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.

    abstract：:The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. In the reproductive system, despite Gsα being associated with oocyte meiotic arres...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.07.023

    authors： Xie Y,Wu B,Jin Y,Zhang A,Sun X,Zhang X,Gao X,Dong R,Li H,Gao J

    更新日期：2018-09-15 00:00:00

  • Overexpression of soluble ADAM33 promotes a hypercontractile phenotype of the airway smooth muscle cell in rat.

    abstract：:A disintegrin and metalloproteinase 33 (ADAM33) has been identified as a susceptibility gene for asthma, but details of the causality are not fully understood. We hypothesize that soluble ADAM33 (sADAM33) overexpression can alter the mechanical behaviors of airway smooth muscle cells (ASMCs) via regulation of the cell...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.10.004

    authors： Duan Y,Long J,Chen J,Jiang X,Zhu J,Jin Y,Lin F,Zhong J,Xu R,Mao L,Deng L

    更新日期：2016-11-15 00:00:00

  • Antibodies to phosphotyrosine injected in Xenopus laevis oocytes modulate maturation induced by insulin/IGF-I.

    abstract：:Xenopus oocytes carry IGF-I receptors, and undergo meiotic maturation in response to binding of IGF-I or insulin to the IGF-I receptor. Maturation is initiated upon activation of the IGF-I receptor tyrosine kinase and requires tyrosine dephosphorylation of p34cdc2, the kinase component of maturation promoting factor (...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90508-r

    authors： Hainaut P,Giorgetti S,Kowalski A,Ballotti R,Van Obberghen E

    更新日期：1991-07-01 00:00:00

  • PC13 embryonal carcinoma cells produce a heparin-binding growth factor.

    abstract：:A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90190-x

    authors： van Veggel JH,van Oostwaard TM,de Laat SW,van Zoelen EJ

    更新日期：1987-04-01 00:00:00

  • Antiapoptotic action of 1,25-dihydroxyvitamin D3 is associated with increased mitochondrial MCL-1 and RAF-1 proteins and reduced release of cytochrome c.

    abstract：:Release of mitochondrial cytochrome c has been recently linked to the activation of the "executioner" phase of the cellular programs for death by apoptosis. This release is known to be negatively regulated by Bcl-2 and Bcl-XL proteins. We show here that treatment of human leukemia cells HL60 with 1,25-dihydroxyvitamin...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3667

    authors： Wang X,Studzinski GP

    更新日期：1997-08-25 00:00:00

  • Active RB elicits late G1/S inhibition.

    abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5510

    authors： Angus SP,Fribourg AF,Markey MP,Williams SL,Horn HF,DeGregori J,Kowalik TF,Fukasawa K,Knudsen ES

    更新日期：2002-06-10 00:00:00

  • Mitochondrial membrane potential and DNA stainability in human sperm cells: a flow cytometry analysis with implications for male infertility.

    abstract：:Sperm cells from control donors of proven fertility and men from barren couples were studied by conventional procedures, i.e., light microscopy as well as flow cytometry. Light microscopy analysis of semen included the measurement of spermatozoa concentration, morphology, and motility. All the men from barren couples ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4064

    authors： Troiano L,Granata AR,Cossarizza A,Kalashnikova G,Bianchi R,Pini G,Tropea F,Carani C,Franceschi C

    更新日期：1998-06-15 00:00:00