Abstract:
:Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenovirus of E2F1, E2F2, E2F3, cyclin E/cdk2, and Mdm2 individually resulted in DNA synthesis in 10-30% of cells. However, combination of Mdm2 with E2F or cyclin E/cdk2 resulted in 50 to 75% of cells synthesizing DNA. DNA synthesis occurred approximately 30 h following infection. We conclude that expression of normal cellular factors is sufficient to induce DNA synthesis in senescent normal human fibroblasts.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Sarraj S,Farb R,Martell REdoi
10.1006/excr.2001.5352keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
268-76issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(01)95352-2journal_volume
270pub_type
杂志文章abstract::Growth factor activity was partially purified from mouse liver plasma membranes and its growth-stimulatory action on cultured mouse fibroblasts was studied. The plasma membrane-associated growth factor (PMGF) was unable to support the proliferation of mouse fibroblasts in monolayer when added as the sole source of gro...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(87)90126-1
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abstract:BACKGROUND:Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to...
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abstract::Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nu...
journal_title:Experimental cell research
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abstract::The mechanisms that structure the mammalian endoplasmic reticulum (ER) network are not fully understood. Here we show that salt extraction of semi-intact normal rat kidney (NRK) fibroblasts and subsequent incubation of the extracted cells with ATP resulted in dramatic ER network retraction. Under these conditions, add...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2008-08-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.04.014
更新日期:2008-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90275-6
更新日期:1983-08-01 00:00:00
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journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2007.10.011
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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abstract::Growth factor-mediated stimulation of epithelial cells induces the disassembly of E-cadherin-mediated cell-cell adhesion. We found that overexpression of a disintegrin and metalloprotease 9 (ADAM9) enhanced growth factor-mediated induction of endocytosis and dynamic recycling of E-cadherin in HT29 human colon cancer c...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2005.10.032
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:2007-06-10 00:00:00