Reconstitution of dna synthetic capacity in senescent normal human fibroblasts by expressing cellular factors E2F and Mdm2.

Abstract:

:Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenovirus of E2F1, E2F2, E2F3, cyclin E/cdk2, and Mdm2 individually resulted in DNA synthesis in 10-30% of cells. However, combination of Mdm2 with E2F or cyclin E/cdk2 resulted in 50 to 75% of cells synthesizing DNA. DNA synthesis occurred approximately 30 h following infection. We conclude that expression of normal cellular factors is sufficient to induce DNA synthesis in senescent normal human fibroblasts.

journal_name

Exp Cell Res

authors

Sarraj S,Farb R,Martell RE

doi

10.1006/excr.2001.5352

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

268-76

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(01)95352-2

journal_volume

270

pub_type

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