Phosphorylation of NPA58, a rat nuclear pore-associated protein, correlates with its mitotic distribution.

abstract：:Laminins assemble into trimers composed of alpha, beta, and gamma chains which posttranslationally are glycosylated and sometimes proteolytically cleaved. In the current paper we set out to characterize posttranslational modifications and the laminin isoforms formed by laminin alpha1 and alpha5 chains. Comparative pul...

journal_title：Experimental cell research

authors： Champliaud MF,Virtanen I,Tiger CF,Korhonen M,Burgeson R,Gullberg D

更新日期：2000-09-15 00:00:00

abstract：:Hematopoiesis is the hierarchical process in which all lineages of blood cells are produced by self-renewing hematopoietic stem cells (HSCs) in the bone marrow (BM). While the regulatory factors that maintain proper HSC function and lineage output under normal conditions are well understood, significantly less is know...

journal_title：Experimental cell research

authors： Mirantes C,Passegué E,Pietras EM

更新日期：2014-12-10 00:00:00

abstract：:Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated nucleotides has been recently proposed as a means of analyzing DNA replication and detecting proliferating cells. The presence of numerous in situ DNA strand breaks is also an inherent feature of apoptotic cells. T...

journal_title：Experimental cell research

authors： Li X,Melamed MR,Darzynkiewicz Z

更新日期：1996-01-10 00:00:00

abstract：:An in vitro system has been employed to study the apoptotic mechanisms in the AK-5 tumor which is a spontaneously regressing rat histiocytoma. Cytosolic extracts of tumor cells primed for apoptosis using dexamethasone and immune serum from tumor-regressing animals were able to induce apoptosis in intact nuclei and rep...

journal_title：Experimental cell research

authors： Anjum R,Khar A

更新日期：1997-11-01 00:00:00

abstract：:Cells with and without hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity were used to examine the transfer of purine metabolites through the medium and via cell contacts. HGPRT- Chinese hamster and human fibroblasts were able to incorporate 3H-labeled purine metabolite(s) from medium in which mouse HGPRT...

journal_title：Experimental cell research

authors： Bols NC,Mosser DD,Boliska SA

更新日期：1987-11-01 00:00:00

abstract：:Long-term biological effects of epidermal growth factor (EGF), insulin, insulin-like growth factor-I (IGF-I), and transforming growth factor-beta (TGF-beta) were examined with human epidermoid carcinoma KB cells. EGF inhibited the growth of KB cells in both serum-containing and serum-free synthetic media by reducing t...

journal_title：Experimental cell research

authors： Koyasu S,Kadowaki T,Nishida E,Tobe K,Abe E,Kasuga M,Sakai H,Yahara I

更新日期：1988-05-01 00:00:00

abstract：:Abnormal aspartyl residue formation such as L-isoaspartates occurs frequently during aging in long-lived proteins, resulting in the alteration of their structures and biological functions. In this study, we investigated the alteration of aspartyl residues in extracellular matrix (ECM) proteins, type-I collagen and fib...

journal_title：Experimental cell research

authors： Lanthier J,Desrosiers RR

更新日期：2004-02-01 00:00:00

abstract：:Autophagy is a cellular pathway involved in protein and organelle degradation. It is relevant to many types of cellular homeostasis and human diseases. High level of glucose is known to inflict podocyte injury, but little is reported about the relationship between high concentrations of glucose and autophagy in these ...

journal_title：Experimental cell research

authors： Ma T,Zhu J,Chen X,Zha D,Singhal PC,Ding G

更新日期：2013-04-01 00:00:00

abstract：:The role of glycans in the apical targeting of proteins in epithelial cells remains a debated question. We have expressed the mouse soluble dipeptidyl peptidase IV (DPP IV ectodomain) in kidney (MDCK) and in intestinal (Caco-2) epithelial cell lines, as a model to study the role of glycosylation in apical targeting. T...

journal_title：Experimental cell research

authors： Slimane TA,Lenoir C,Sapin C,Maurice M,Trugnan G

更新日期：2000-07-10 00:00:00

abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

journal_title：Experimental cell research

authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

更新日期：1990-09-01 00:00:00

abstract：:Cancer cell survival is frequently dependent on the elevated levels of members of the Bcl-2 family of prosurvival proteins that bind to and inactivate BH3-domain pro-apoptotic cellular proteins. Small molecules that inhibit the protein-protein interactions between prosurvival and proapoptotic Bcl-2 family members (so-...

journal_title：Experimental cell research

authors： Modugno M,Banfi P,Gasparri F,Borzilleri R,Carter P,Cornelius L,Gottardis M,Lee V,Mapelli C,Naglich JG,Tebben A,Vite G,Pastori W,Albanese C,Corti E,Ballinari D,Galvani A

更新日期：2015-03-15 00:00:00

abstract：:TGFβ contributes to mesangial cell hypertrophy and matrix protein increase in various kidney diseases including diabetic nephropathy. Deptor is an mTOR-interacting protein and suppresses mTORC1 and mTORC2 activities. We have recently shown that TGFβ-induced inhibition of deptor increases the mTOR activity. The mechani...

journal_title：Experimental cell research

authors： Maity S,Bera A,Ghosh-Choudhury N,Das F,Kasinath BS,Choudhury GG

更新日期：2018-03-01 00:00:00

abstract：:The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...

journal_title：Experimental cell research

authors： Leemann U,Ruch F

更新日期：1983-09-01 00:00:00

abstract：:Abrupt developmental changes occur in structural form and function of connexin (Cx) channels in the mouse mammary gland. Microarray study shows that the principal connexin isoform in epithelial cells during pregnancy is Cx26, up-regulated and persisting from the virgin. After parturition, there is rapid induction of C...

journal_title：Experimental cell research

authors： Locke D,Stein T,Davies C,Morris J,Harris AL,Evans WH,Monaghan P,Gusterson B

更新日期：2004-08-15 00:00:00

abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

journal_title：Experimental cell research

authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

更新日期：1998-05-25 00:00:00

abstract：:A temperature-sensitive mutant, tsJT60, grew exponentially at both 34 degrees and 39.5 degrees C, but when stimulated from the resting state it entered S phase at 34 degrees but not at 39.5 degrees C. The mutated function appeared to be a prerequisite throughout from 0 to 9 h following the stimulation, in order that G...

journal_title：Experimental cell research

authors： Ninomiya-Tsuji J,Goto Y,Ishibashi S,Ide T

更新日期：1986-07-01 00:00:00

abstract：:The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...

journal_title：Experimental cell research

authors： Shiraga T,Winpenny JP,Carter EJ,McCarthy VA,Hollingsworth MA,Harris A

更新日期：2005-08-01 00:00:00

abstract：:The alpha 3 beta 1 integrin has been implicated as a receptor for several matrix components, including collagen, fibronectin, and laminins. The function of alpha 3 beta 1 seems to be very versatile involving cell adhesion to or migration on ECM, establishment of cell-cell contacts in aggregates, as well as linkage to ...

journal_title：Experimental cell research

authors： Lichtner RB,Howlett AR,Lerch M,Xuan JA,Brink J,Langton-Webster B,Schneider MR

更新日期：1998-05-01 00:00:00

abstract：:Mechanical stress is an important regulator of chondrocyte functions but the mechanisms by which chondrocytes sense mechanical signals are unknown. Receptors for matrix molecules are likely involved in the mechanical signaling. In the first part of this study we identified integrins with affinity for the cartilage-spe...

journal_title：Experimental cell research

authors： Holmvall K,Camper L,Johansson S,Kimura JH,Lundgren-Akerlund E

更新日期：1995-12-01 00:00:00

abstract：:Uterine fibroids, also known as uterine leiomyomas, are a benign tumor of the human uterus and the commonest estrogen-dependent benign tumor found in women. Myocardin is an important transcriptional regulator in smooth and cardiac muscle development. The role of myocardin and its relationship with ERα in uterine fibro...

journal_title：Experimental cell research

authors： Liao XH,Li JY,Dong XM,Wang X,Xiang Y,Li H,Yu CX,Li JP,Yuan BY,Zhou J,Zhang TC

更新日期：2017-01-01 00:00:00

abstract：:Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the ...

journal_title：Experimental cell research

authors： DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K

更新日期：2002-03-10 00:00:00

abstract：:Osteoclasts are postmitotic, multinucleated giant cells generated by the fusion of hematopoietic mononuclear precursors from the monocyte-macrophage lineage. In culture, adherent macrophages from blood-derived monocytes grow, gather, and fuse together to form multinucleated osteoclast-like cells. These events are cont...

journal_title：Experimental cell research

authors： Machuca I,Domenget C,Jurdic P

更新日期：1999-08-01 00:00:00

abstract：:The Nir/rdgB family of proteins has been identified in a variety of eukaryotic organisms, ranging from worms to mammals. The Drosophila retinal degeneration B (rdgB), a protein that is required for photoreceptor cell viability and light response, was the first to be identified. It consists an amino-terminal phosphatid...

journal_title：Experimental cell research

authors： Lev S

更新日期：2004-07-01 00:00:00

abstract：:A ts mutant clone, tsJT60, was isolated from Fisher rat cell line, 3Y1. During the exponential growth at both 34 and 39.5 degrees C, tsJT60 did not appear as ts mutant cells. However, once entered resting state (G0) under serum deprivation at the confluent state, they could re-enter S phase at 34 degrees C but could n...

journal_title：Experimental cell research

authors： Ide T,Ninomiya J,Ishibashi S

更新日期：1984-01-01 00:00:00

abstract：:We report the isolation of six cell lines (designated EB cell lines) from cultures of the hypoxanthine guanine phosphoribosyl transferase-deficient (HGPRT-) feeder-dependent embryonal carcinoma cell line PSA4TG12 which have undergone in vitro differentiation, and of clonal derivatives of these lines. Whereas some line...

journal_title：Experimental cell research

authors： Morgan RH,Henry JA,Hooper ML

更新日期：1983-10-15 00:00:00

abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

journal_title：Experimental cell research

authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

更新日期：2012-03-10 00:00:00

abstract：:This study shows the sensitivity of insulin-secreting RIN m5F cells to undergoing apoptosis without modifying intracellular free calcium concentrations. The culture of cells in the absence of serum during 6 h failed to produce spontaneous DNA fragmentation. However, when cultures were carried out in the presence of th...

journal_title：Experimental cell research

authors： Sánchez-Margalet V,Lucas M,Solano F,Goberna R

更新日期：1993-11-01 00:00:00

abstract：:Autophagy has been shown to be critically associated with the central mechanisms underlying Parkinson's disease (PD), while the mechanisms contributing to the imbalance of autophagy remain unclear. Small nucleolar RNA host gene 1 (SNHG1), a well-studied long noncoding RNA, has been reported to be significantly increas...

journal_title：Experimental cell research

authors： Qian C,Ye Y,Mao H,Yao L,Sun X,Wang B,Zhang H,Xie L,Zhang H,Zhang Y,Zhang S,He X

更新日期：2019-11-01 00:00:00

abstract：:p107 and p130 were originally identified as targets of the transforming domains of viral oncoproteins encoded by small DNA tumor viruses. Together with pRB, the protein product of the retinoblastoma gene (Rb), p107 and p130 represent a family of closely related proteins that play critical roles in the regulation of ce...

journal_title：Experimental cell research

authors： Classon M,Dyson N

更新日期：2001-03-10 00:00:00

abstract：:LANA2 is a latent protein detected in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected B cells that inhibits p53-dependent transcriptional transactivation and apoptosis and PKR-dependent apoptosis, suggesting an important role in the transforming activity of the virus. It has been reported that LANA2 localizes ...

journal_title：Experimental cell research

authors： Muñoz-Fontela C,Collado M,Rodriguez E,García MA,Alvarez-Barrientos A,Arroyo J,Nombela C,Rivas C

更新日期：2005-11-15 00:00:00