Identification of a nuclear export signal in the KSHV latent protein LANA2 mediating its export from the nucleus.

Abstract:

:LANA2 is a latent protein detected in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected B cells that inhibits p53-dependent transcriptional transactivation and apoptosis and PKR-dependent apoptosis, suggesting an important role in the transforming activity of the virus. It has been reported that LANA2 localizes into the nucleus of both KSHV-infected B cells and transiently transfected HeLa cells. In this study, we show that LANA2 is a nucleocytoplasmic shuttling protein that requires a Rev-type nuclear export signal located in the C-terminus to direct the protein to the cytoplasm, through an association with the export receptor CRM1. In addition, a functional protein kinase B (PKB)/Akt phosphorylation motif partially overlapping with the nuclear export signal was identified. Nuclear exclusion of LANA2 was negatively regulated by the phosphorylation of threonine 564 by Akt. The ability of LANA2 to shuttle between nucleus and cytoplasm has implications for the function of this viral protein.

journal_name

Exp Cell Res

authors

Muñoz-Fontela C,Collado M,Rodriguez E,García MA,Alvarez-Barrientos A,Arroyo J,Nombela C,Rivas C

doi

10.1016/j.yexcr.2005.08.022

keywords:

subject

Has Abstract

pub_date

2005-11-15 00:00:00

pages

96-105

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(05)00390-3

journal_volume

311

pub_type

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