Abstract：
:Long-term biological effects of epidermal growth factor (EGF), insulin, insulin-like growth factor-I (IGF-I), and transforming growth factor-beta (TGF-beta) were examined with human epidermoid carcinoma KB cells. EGF inhibited the growth of KB cells in both serum-containing and serum-free synthetic media by reducing the growth rate and by lowering the saturation density. The cells cultured with EGF showed relatively high motility and grew dispersely as single cells, whereas the cells cultured in the absence of EGF grew in clusters. Although TGF-beta itself did not inhibit the growth of KB cells, it augmented the growth inhibition by EGF. TGF-beta also affected the cell morphology. In the presence of TGF-beta, the cells became flattened and actin stress fibers were well developed compared to those cultured in its absence. The effects of EGF on growth, cell motility, and cell morphology were reversible. Tyrosine phosphorylation of EGF receptors was continuously observed for at least 50 h in the presence of EGF. TGF-beta did not increase the phosphorylation induced by EGF. These results suggested that signals continuously transmitted through EGF receptors caused the changes in cell growth and morphology and that TGF-beta did not act on the cells by modulating binding of EGF to its receptors or activation of the receptor kinase. In contrast to EGF and TGF-beta, neither insulin nor IGF-I affected cell morphology or growth, although KB cells express their receptors and the receptor kinases were also continuously activated during exposure of the cells to insulin or IGF-I.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Koyasu S,Kadowaki T,Nishida E,Tobe K,Abe E,Kasuga M,Sakai H,Yahara Idoi
10.1016/0014-4827(88)90125-5subject
Has Abstractpub_date
1988-05-01 00:00:00pages
107-16issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(88)90125-5journal_volume
176pub_type
杂志文章abstract：:The Cdc25 phosphatases play crucial roles in cell cycle progression by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Cdc25A is an important regulator of the G1/S transition but functions also in the mitotic phase of the human cell cycle. In this paper, we investigate ...
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pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：1986-01-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1991-01-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2010-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90034-8
更新日期：1990-11-01 00:00:00
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doi：10.1016/0014-4827(88)90472-7
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journal_title：Experimental cell research
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doi：10.1016/0014-4827(90)90182-a
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journal_title：Experimental cell research
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doi：10.1006/excr.1996.0159
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2004-11-15 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1163
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVES:Vascular calcification is highly prevalent in patients with chronic kidney disease (CKD) and contributes to increased risk of cardiovascular disease and mortality. Accumulated evidences suggested that vascular smooth muscle cells (VSMCs) to osteoblast-like cells transdifferentiation (VOT) plays a crucial rol...
journal_title：Experimental cell research
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更新日期：2014-04-01 00:00:00
