Abstract：
:Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appearing on the basal side within a 60-min incubation at 37 degrees C. Other substances such as heparin were transported only minimally, whereas horseradish peroxidase was transported to the same degree in both directions. Greater than 90% of the transported IgG was precipitable by trichloroacetic acid and 81% was capable of binding to protein G. Such a rapid transport of large amounts of IgG in trophoblast cells is consistent with a receptor-mediated process of transcytosis.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Sooranna SR,Contractor SFdoi
10.1016/0014-4827(91)90154-msubject
Has Abstractpub_date
1991-01-01 00:00:00pages
41-5issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(91)90154-Mjournal_volume
192pub_type
杂志文章abstract：:AP-1/cJun, NF-kappaB and STAT3 transcription factors control expression of numerous genes, which regulate critical cell functions including proliferation, survival and apoptosis. Sodium arsenite is known to suppress both the IKK-NF-kappaB and JAK2-STAT3 signaling pathways and to activate the MAPK/JNK-cJun pathways, th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.09.019
更新日期：2006-12-10 00:00:00
abstract：:The effects of non-drug-containing liposomes of different compositions and sizes on the proliferation of nine cancer-derived and one normal cultured human cell lines were determined. Stearylamine- and cardiolipin-containing liposomes were toxic (ID50) at 200 microM liposomal lipid concentrations or less, whereas phosp...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90262-x
更新日期：1987-07-01 00:00:00
abstract：:Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3891
更新日期：1998-02-25 00:00:00
abstract：:In the acute promyelocytic leukemia cell line NB4, Bcl-2 downregulation occurred as a late event of retinoid-induced differentiation. In the maturation-resistant NB4-R1 subclone, retinoids failed to downregulate Bcl-2 even in the situation of apoptosis massively induced by pan-agonists and RXR-selective agonists. We o...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3594
更新日期：1997-06-15 00:00:00
abstract：:In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90330-8
更新日期：1988-06-01 00:00:00
abstract：:Germ cells were isolated from mouse fetal gonads 11 1/2-16 1/2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1/2 dpc onwards, both male and female germ cells survived well at 37 degrees C for several days. During the culture period the proportion of female germ cells in meiosis in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90421-4
更新日期：1983-04-01 00:00:00
abstract：:This review article describes the pathways and mechanisms of endocytosis and post-endocytic sorting of the EGF receptor (EGFR/ErbB1) and other members of the ErbB family. Growth factor binding to EGFR accelerates its internalization through clathrin-coated pits which is followed by the efficient lysosomal targeting of...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2008.07.029
更新日期：2009-02-15 00:00:00
abstract：:Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90502-6
更新日期：1985-11-01 00:00:00
abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90136-x
更新日期：1990-09-01 00:00:00
abstract：:Cultured megakaryocytes, isolated from guinea pig bone marrow, were treated with buffer or adenosine diphosphate (ADP) (10 microM) on plain or coated glass surfaces. Control cells were rounded and non-adherent. The nucleotide induced the cells to spread to several times the initial diameter, and to become flattened an...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90649-9
更新日期：1984-06-01 00:00:00
abstract：:β-catenin is a key mediator of Wnt signaling and its deregulated nuclear accumulation can drive cancer progression. While the central armadillo (Arm) repeats of β-catenin stimulate nuclear entry, the N- and C-terminal "tail" sequences are thought to regulate turnover and transactivation. We show here that the N- and C...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.09.009
更新日期：2016-11-01 00:00:00
abstract：:We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(02)00036-8
更新日期：2003-04-01 00:00:00
abstract：:We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3552
更新日期：1997-05-25 00:00:00
abstract：:Elevated plasma free fatty acids level has been implicated in the development of insulin resistance, inflammation, and endothelial dysfunction in diabetic and nondiabetic individuals. However, the underlying mechanisms still remain to be defined. Herein, we investigated the effect of palmitic acid (PA), the most abund...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111552
更新日期：2019-10-15 00:00:00
abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.03.042
更新日期：2017-05-15 00:00:00
abstract：:We recently observed a 25-fold increase in the activity of ornithine decarboxylase (ODC) 6 h after treatment of G1-synchronized CTLL-2 cells with interleukin-2 (IL-2). Here we show that the ODC mRNA content increased in parallel with the ODC activity during the first hours of stimulation with IL-2, resulting in a 25-f...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90201-2
更新日期：1989-03-01 00:00:00
abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2012.02.026
更新日期：2012-05-15 00:00:00
abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.07.016
更新日期：2004-12-10 00:00:00
abstract：:Swiss 3T3 cells can be made quiescent at low density by plating in medium MCDB 402 supplemented with dexamethasone (DEX), insulin (INS) and bovine plasma fibronectin (BPFn) for 3 days. One hour after stimulation of these cells by fibroblast growth factor (FGF), an increase in the rate of protein synthesis can be measu...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90128-3
更新日期：1983-07-01 00:00:00
abstract：:The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophob...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3603
更新日期：1997-07-10 00:00:00
abstract：:Chronic lymphocytic leukemia lymphocytes were used to study mechanisms involved in apoptosis (programmed cell death). Apoptosis, which was determined by morphological changes including cell death and by internucleosomal DNA fragmentation, occurred during culture for 1 to 2 days in a portion of the cells from three of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90393-m
更新日期：1992-02-01 00:00:00
abstract：:Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.01.025
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:Owing to the late diagnosis and frequent metastasis, ovarian cancer (OC) exhibits a high mortality rate. The study was intended to figure out the function of long non-coding RNA (lncRNA) HCP5 in OC metastasis. METHODS:Microarray analysis was conducted to probe aberrantly expressed lncRNAs in OC tissues. Artifi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112129
更新日期：2020-09-01 00:00:00
abstract：:We have compared the rate of synthesis, half-life, and steady-state content of the oncoprotein p53 in logarithmically growing cultures of (a) primary embryo, (b) immortalized but untransformed, and (c) spontaneously transformed mouse fibroblasts. Steady-state p53 content derived from metabolic labeling and immunopreci...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90331-5
更新日期：1989-10-01 00:00:00
abstract：:The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4312
更新日期：1999-02-01 00:00:00
abstract：:The adenine nucleotide translocator-2 (ANT2) gene is expressed in growth-activated cells together with the early-immediate genes. We have studied the mechanism of ANT2 expression during the serum-induced transition from G0 to G1 and during reentry into G0 as cells approach confluence. Actinomycin D completely blocked ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4432
更新日期：1999-05-01 00:00:00
abstract：:Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.02.024
更新日期：2006-07-01 00:00:00
abstract：:The Rab11 family of small GTPases is composed of three members, Rab11a, Rab11b, and Rab25. While recent work on Rab11a and Rab25 has yielded some insights into their function, Rab11b has received little attention. Therefore, we sought to examine the distribution of endogenous Rab11b in epithelial cells. In rabbit gast...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00340-9
更新日期：2003-11-01 00:00:00
abstract：:Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.07.003
更新日期：2010-12-10 00:00:00
abstract：:Oxysterol-binding protein (OSBP)-related proteins (ORPs) are conserved lipid binding proteins found in organisms ranging from yeast to mammals. Recent findings have indicated that these proteins mainly localize to contact sites of 2 different membranous organelles. ORP6, a member of the ORP subfamily III, is one of th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.07.025
更新日期：2018-09-15 00:00:00
