Abstract:
:We have compared the rate of synthesis, half-life, and steady-state content of the oncoprotein p53 in logarithmically growing cultures of (a) primary embryo, (b) immortalized but untransformed, and (c) spontaneously transformed mouse fibroblasts. Steady-state p53 content derived from metabolic labeling and immunoprecipitation data revealed either no change or only a slight decrease (up to 1.5-fold depending on the antibody used) in transformed cells compared with immortal or primary cultures, p53 showed the same short half-life in all cell types. In contrast, immunocytochemical analysis of p53 content in intact cells demonstrated an increase in the proportion of cells with detectable nuclear p53 from approximately 4% in primary and immortal cultures to approximately 10% in fully transformed cells, together with a marked increase in the intensity of nuclear positivity. We suggest that transformation is associated with an increase in the cellular content of p53 in a subcellular pool which was not detectable in detergent for immunoprecipitation. In addition, immunocytochemical analysis demonstrated a marked heterogeneity in p53 content in all cell types which was not related to clonal variation, cell cycle phase, or growth state. These data challenge previous suggestion regarding the role of p53 in growth control.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Williams NW,Wynford-Thomas Ddoi
10.1016/0014-4827(89)90331-5subject
Has Abstractpub_date
1989-10-01 00:00:00pages
316-28issue
2eissn
0014-4827issn
1090-2422journal_volume
184pub_type
杂志文章abstract::Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.02.009
更新日期:2005-05-15 00:00:00
abstract::Actions of transforming growth factor-β are largely context dependent. For instance, TGF-β is growth inhibitory to epithelial cells and many tumor cell-lines while it stimulates the growth of mesenchymal cells. TGF-β also activates fibroblast cells to a myofibroblastic phenotype. In order to understand how the respons...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.03.001
更新日期:2015-06-10 00:00:00
abstract::A two-site radio-immunoassay for beta NGF demonstrated 5-10 pg of NGF in the normal, adult rat iris. Ciliarectomy or sympathectomy did not significantly alter the amount of NGF after 10 days. However, denervation including all sensory axons (stereotactic lesion distal to the trigeminal ganglion) increased the level to...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90155-6
更新日期:1983-10-15 00:00:00
abstract:BACKGROUND:Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast afte...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.03.056
更新日期:2017-06-15 00:00:00
abstract::The turbidity of axonemes during active sliding of microtubules was analysed using the stopped-flow-light-scattering method with high time resolution. Flagella of sea-urchin spermatozoa were demembranated and used after a brief treatment with trypsin. The turbidity of the suspension of flagellar axonemes during ATP-in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90571-9
更新日期:1986-03-01 00:00:00
abstract::Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.09.012
更新日期:2008-01-15 00:00:00
abstract::Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90169-2
更新日期:1986-12-01 00:00:00
abstract::The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.02.028
更新日期:2014-05-01 00:00:00
abstract::There is increasing evidence that basic fibroblast growth factor (bFGF) plays an important role in cell proliferation, differentiation, and survival in various systems. In the eye, although a truncated, dominant negative bFGF receptor in transgenic mice induced defective lens development and caused lens fiber cells to...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4450
更新日期:1999-05-25 00:00:00
abstract::Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.04.028
更新日期:2019-07-15 00:00:00
abstract::We report that a fatty acid auxotroph of Tetrahymena thermophila (RH179E1) fails to mate, yet retains the ability to co-stimulate normal cells unidirectionally. Thus, co-stimulation can be analyzed experimentally in the absence of pair formation. We show that the co-stimulation of normal cells of one mating type is su...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90225-4
更新日期:1987-03-01 00:00:00
abstract::Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90554-8
更新日期:1991-03-01 00:00:00
abstract::Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.08.021
更新日期:2016-10-15 00:00:00
abstract::During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.10.004
更新日期:2007-01-01 00:00:00
abstract::Mutations in the tumor suppressor gene p53 were found in more than 90% of all human squamous cell carcinomas (SCC). To study the function of p53 in a keratinocyte background, a tetracycline-controlled p53 transgene was introduced into a human SCC cell line (SCC15), lacking endogenous p53. Conditional expression of wil...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.07.016
更新日期:2005-10-15 00:00:00
abstract::Keratinocytes in human skin undergo apoptosis during various inflammatory processes and after ultraviolet (UV) irradiation. To determine if keratinocyte apoptosis may be mediated by the Fas/APO-1 receptor (CD95), a signal transduction pathway known to initiate programmed cell death of lymphocytes, we investigated Fas ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3514
更新日期:1997-05-01 00:00:00
abstract::Rat pheochromocytoma cells, PC12 cells, undergo differentiation in response to nerve growth factor (NGF). Although the Ras-MAP kinase signaling pathway has been shown to play a central role in the response to NGF, the precise mechanism which induces differentiation remains unclarified. Recently, several gamma-lactam-r...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3615
更新日期:1997-08-01 00:00:00
abstract::Ring finger protein 6 (RNF6), a RING-domain E3 ubiquitin Ligase, functions as a potential tumor promoter in several caners. However, the function and clinical significance of RNF6 in hepatocellular carcinoma (HCC) remains unclear. Here, we found that high RNF6 expression was detected frequently in primary HCC tissues,...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.11.019
更新日期:2019-01-01 00:00:00
abstract::We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...
journal_title:Experimental cell research
pub_type: 杂志文章,收录出版
doi:10.1016/j.yexcr.2016.07.002
更新日期:2016-09-10 00:00:00
abstract::Interference of colchicine with meiotic chromosome pairing in the wild garlic, Allium ursinum, was studied using a whole-mount spreading technique for synaptonemal complexes. Colchicine was found to cause (i) pairing suppression (arrest of leptotene) and (ii) deficient pairing initiation at zygotene in connection with...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90381-3
更新日期:1988-09-01 00:00:00
abstract::High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.01.014
更新日期:2012-03-10 00:00:00
abstract::Changes in the expression pattern of DNA polymerase beta gene during rat lung, brain, and testis development have been investigated. A decrease in the level of beta-pol mRNA was observed during postnatal development of lung and brain. By contrast, an almost 20-fold increase in the level of beta-pol mRNA was observed d...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90034-8
更新日期:1990-11-01 00:00:00
abstract::The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00386-0
更新日期:2003-11-15 00:00:00
abstract::At the onset of mitosis in higher eukaryotic cells, the nuclear envelope and its components including subunits of the nuclear pore complexes are disassembled, and these are reassembled toward the end of mitosis. We have studied the role of protein phosphorylation in this process, by investigating the phosphorylation s...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5052
更新日期:2000-11-25 00:00:00
abstract::Treatment with ionic detergents of nuclei isolated from various continuously growing cell lines generally yields chromatin samples of high viscosity. Extensive treatment with nuclease-free proteinase K or pronase solubilized the viscous lysates with > 90% of the DNA migrating at approximately 50 kb. Freshly prepared h...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1381
更新日期:1995-12-01 00:00:00
abstract::During mitosis there is an abrupt inhibition of a wide range of membrane functions, including fluid-phase and adsorptive pinocytosis. We have used cell hybrids formed between mitotic and interphase cells to approach the mechanism of this inhibition. We report that fluid pinocytosis is reactivated in the mitotic partne...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90178-9
更新日期:1987-08-01 00:00:00
abstract::We have studied the internalization of targeted fusogenic liposome content to leukemic T cells (CEM) in vitro. We describe a method for the covalent coupling of T101 antibody to the surface of liposomes and the incorporation of fusogenic viral protein into the liposome membrane. Hygromycin B, an impermeant inhibitor o...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90042-6
更新日期:1989-11-01 00:00:00
abstract::VEGF is a major inducer of angiogenesis. However, the homing role of VEGF for cardiac stem cells (CSCs) is unclear. In in vitro experiments, CSCs were isolated from the rat hearts, and a cellular migration assay was performed using a 24-well transwell system. VEGF induced CSC migration in a concentration-dependent man...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.09.026
更新日期:2009-12-10 00:00:00
abstract::During pathological bone loss, factors that are both stimulatory and inhibitory for osteoclast differentiation are over-expressed. Despite the presence of inhibitory factors, osteoclast differentiation is significantly enhanced to bring about bone loss. To examine the hypothesis that stimulatory growth factors overcom...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.11.009
更新日期:2004-03-10 00:00:00
abstract::Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.03.013
更新日期:2007-05-15 00:00:00