Induction of neurite outgrowth in PC12 cells by gamma-lactam-related compounds via Ras-MAP kinase signaling pathway independent mechanism.

Abstract:

:Rat pheochromocytoma cells, PC12 cells, undergo differentiation in response to nerve growth factor (NGF). Although the Ras-MAP kinase signaling pathway has been shown to play a central role in the response to NGF, the precise mechanism which induces differentiation remains unclarified. Recently, several gamma-lactam-related microbial products were identified to induce neurite outgrowth in neuroblastoma cells. Therefore, we synthesized a series of gamma-lactam-related compounds and tested for their ability to induce neurite outgrowth in PC12 cells. We found that two compounds, MT-19 and MT-20, induced neurite outgrowth at concentrations as low as 1 microg/ml. MT-19 and MT-20 have an n-hexadecyl group and an n-dodecyl group, respectively, at the position N-1 of the gamma-lactam ring, and the modification of this group leads to partial or complete loss of activity. In addition, the modification of the methyl and hydroxyl group at C-5 leads to complete loss of activity, indicating a strict structure-activity relationship. Interestingly, MT-19 and MT-20 induced neurite outgrowth of PC12 cells which lack normal Ras function. Furthermore, these compounds did not induce MAP kinase activation, suggesting that MT-19 and MT-20 do not require the Ras-MAP kinase signaling pathway which is shown to be necessary and sufficient for NGF-induced neurite outgrowth. Consistent with this, none of the early- or late-response genes tested, which include fos, zif268, Nur77, vgf, and transin, was induced. However, the protein level of three neurofilaments was increased after the incubation with these compounds. Since the level of other cytoskeleton proteins including actin and tubulin remained constant, MT-19 and MT-20 specifically affected neurofilament synthesis and/or turnover. Taken together, these findings indicate that MT-19 and MT-20 induce neurite outgrowth by activating the downstream target of MAP kinase or by a novel mechanism which is distinct from the NGF-activated pathway.

journal_name

Exp Cell Res

authors

Yao R,Osada H

doi

10.1006/excr.1997.3615

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

233-9

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(97)93615-6

journal_volume

234

pub_type

杂志文章
  • The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes.

    abstract::LMX1B is a LIM-homeodomain transcription factor essential for development. Putative LMX1B target genes have been identified through mouse gene targeting studies, but their identity as direct LMX1B targets remains hypothetical. We describe here the first molecular characterization of LMX1B target gene regulation. Micro...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.10.012

    authors: Rascle A,Neumann T,Raschta AS,Neumann A,Heining E,Kastner J,Witzgall R

    更新日期:2009-01-01 00:00:00

  • In vitro culture of mouse primordial germ cells.

    abstract::Germ cells were isolated from mouse fetal gonads 11 1/2-16 1/2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1/2 dpc onwards, both male and female germ cells survived well at 37 degrees C for several days. During the culture period the proportion of female germ cells in meiosis in...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(83)90421-4

    authors: De Felici M,McLaren A

    更新日期:1983-04-01 00:00:00

  • Aging- and growth-dependent modulation of endothelin-1 gene expression in human vascular endothelial cells.

    abstract::Earlier we reported the undetectable level of endothelin (ET)-1 mRNA in aorta from young donors, in contrast to the detectable levels in older donors (Lab. Invest. 67, 210-217, 1992). We also found that the synthesis of ET-1 peptide is elevated in cultured endothelial cells from the aorta of over-50-year-old donors. I...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1994.1051

    authors: Kumazaki T,Fujii T,Kobayashi M,Mitsui Y

    更新日期:1994-03-01 00:00:00

  • The long non-coding RNA ILF3-AS1 increases the proliferation and invasion of retinoblastoma through the miR-132-3p/SMAD2 axis.

    abstract::A great deal of evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of retinoblastoma (RB). However, the roles of lncRNA ILF3-AS1 in RB are still unclear. In the present study, our work revealed that the lncRNA ILF3-AS1 was increased in both RB tissues and cell lines. Repression of ILF3...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2020.112087

    authors: Han S,Song L,Chen Y,Hou M,Wei X,Fan D

    更新日期:2020-08-15 00:00:00

  • Reversible induction of actin rods in mouse C3H-2K cells by incubation in salt buffers and by treatment with non-ionic detergents.

    abstract::Incubating conditions which induced actin paracrystal-like intracellular structures (actin rods) were investigated by using several cell lines. We have found that an incubation of cells of a mouse fibroblastic cell line, C3H-2K, in an isotonic solution of NaCl containing 1 mM MgCl2, 1 mM CaCl2 and 10 mM MES, pH 6.5, i...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90047-9

    authors: Iida K,Yahara I

    更新日期:1986-06-01 00:00:00

  • Resistance of NIH3T3 cells to v-fes transformation induced by a dominant negative H-ras mutant.

    abstract::NIH3T3 cells carrying a dominant negative H-ras mutant 116Y acquired resistance to transformation by some PTK oncogenes, i.e., v-fes, v-abl, and v-fms, but were sensitive to viral ras and serine threonine kinase oncogenes, v-raf and v-mos. One clone, designated 1-20, infected with v-fes (1-20 fes) exhibited flat morph...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1263

    authors: Ogiso Y,Yokoyama T,Watari H,Shih TY,Kuzumaki N

    更新日期:1993-10-01 00:00:00

  • Introducing www.axolotl-omics.org - an integrated -omics data portal for the axolotl research community.

    abstract::Genomic resources are indispensable for biological investigations in model organisms. In recent years, a number of genomic resources including a full genome assembly, extensive transcriptomic data, as well as genome editing has been developed for the axolotl, a classical model organism for developmental, neurobiologic...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2020.112143

    authors: Nowoshilow S,Tanaka EM

    更新日期:2020-09-01 00:00:00

  • Rhodamine 123 uptake and mitochondrial DNA content in rabbit articular chondrocytes evolve differently upon transfer from cartilage to culture conditions.

    abstract::Mitochondrial DNA (mtDNA) represents 0.15% of the total cell DNA (at least an order of magnitude less than in liver or heart) of rabbit articular chondrocytes. Besides the already well-documented low respiratory activity, chondrocyte differentiation thus involves a specific control of mitochondrial biogenesis. When tr...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(87)90172-8

    authors: Champagne AM,Benel L,Ronot X,Mignotte F,Adolphe M,Mounolou JC

    更新日期:1987-08-01 00:00:00

  • Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells.

    abstract::Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription wa...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(88)90397-7

    authors: Egyhazi E,Pigon A,Chang JH,Ghaffari SH,Dreesen TD,Wellman SE,Case ST,Olson MO

    更新日期:1988-10-01 00:00:00

  • Mammalian ChlR1 has a role in heterochromatin organization.

    abstract::The ChlR1 DNA helicase, encoded by DDX11 gene, which is responsible for Warsaw breakage syndrome (WABS), has a role in sister-chromatid cohesion. In this study, we show that human ChlR1 deficient cells exhibit abnormal heterochromatin organization. While constitutive heterochromatin is discretely localized at perinucl...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.08.006

    authors: Inoue A,Hyle J,Lechner MS,Lahti JM

    更新日期:2011-10-15 00:00:00

  • Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation.

    abstract::In cell culture, many adherent mammalian cells undergo substantial actin cytoskeleton rearrangement prior to mitosis as they detach from the extracellular matrix and become spherical. At the end of mitosis, the actin cytoskeleton is required for cytokinesis and the reassembly of interphase structures as cells spread a...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2014.10.024

    authors: Szeto SGY,Williams EC,Rudner AD,Lee JM

    更新日期:2015-01-15 00:00:00

  • Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes.

    abstract::The calcium/calmodulin-dependent protein phosphatase calcineurin is required for the induction of transcriptional events that initiate and promote myogenic differentiation. An important effector for calcineurin in striated muscle is the transcription factor myocyte enhancer factor 2 (MEF2). The targeting of the enzyme...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2012.12.016

    authors: Li J,Vargas MA,Kapiloff MS,Dodge-Kafka KL

    更新日期:2013-02-15 00:00:00

  • Characterization of STIP, a multi-domain nuclear protein, highly conserved in metazoans, and essential for embryogenesis in Caenorhabditis elegans.

    abstract::We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spati...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.01.003

    authors: Ji Q,Huang CH,Peng J,Hashmi S,Ye T,Chen Y

    更新日期:2007-04-15 00:00:00

  • TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner.

    abstract::Satellite cells are a quiescent heterogeneous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-beta (TGF-beta) superfamily members are elevated post-injury and their importance in the r...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.10.037

    authors: Schabort EJ,van der Merwe M,Loos B,Moore FP,Niesler CU

    更新日期:2009-02-01 00:00:00

  • Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells.

    abstract::In many animals, the germ line develops from a distinct mitochondria-rich region of embryonic cytoplasm called the germ plasm. However, the protein composition of germ plasm and its formation remain poorly understood, except in Drosophila. Here, we show that Xpat, a recently identified protein component of Xenopus ger...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.10.019

    authors: Hames RS,Hames R,Prosser SL,Euteneuer U,Lopes CA,Moore W,Woodland HR,Fry AM

    更新日期:2008-02-01 00:00:00

  • Parallels between immune driven-hematopoiesis and T cell activation: 3 signals that relay inflammatory stress to the bone marrow.

    abstract::Quiescence, self-renewal, lineage commitment and differentiation of hematopoietic stem cells (HSCs) towards fully mature blood cells are a complex process that involves both intrinsic and extrinsic signals. During steady-state conditions, most hematopoietic signals are provided by various resident cells inside the bon...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2014.09.016

    authors: Libregts SF,Nolte MA

    更新日期:2014-12-10 00:00:00

  • A newly established porcine aortic endothelial cell line: characterization and application to the study of human-to-swine graft rejection.

    abstract::The establishment of cell lines allows reproductible in vitro studies that would be far more difficult to perform using primary cells that rapidly undergo phenotypical alterations in culture. The purpose of this work was to establish an endothelial cell line appropriate for in vitro study of endothelial cell activatio...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3831

    authors: Malassagne B,Taboit F,Conti F,Batteux F,Atia N,Chéreau C,Conjeaud H,Théron MC,Attal J,Braet F,Houdebine LM,Calmus Y,Houssin D,Weill B

    更新日期:1998-01-10 00:00:00

  • MIST1 regulates the pancreatic acinar cell expression of Atp2c2, the gene encoding secretory pathway calcium ATPase 2.

    abstract::MIST1 is a transcription factor expressed in pancreatic acinar cells and other serous exocrine cells. Mice harboring a targeted deletion of the Mist1 gene (Mist1(-/-)) exhibit alterations in acinar regulated exocytosis and aberrant Ca(2+) signaling that are normally controlled by acinar cell Ca(2+)-ATPases. Previous s...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2010.06.014

    authors: Garside VC,Kowalik AS,Johnson CL,DiRenzo D,Konieczny SF,Pin CL

    更新日期:2010-10-15 00:00:00

  • Homocysteine regulates expression of Herp by DNA methylation involving the AARE and CREB binding sites.

    abstract::Herp (homocysteine-induced endoplasmic reticulum protein) is an ER-resident membrane protein, which has a ubiquitin-like domain at its N-terminus. Expression of Herp protein is up-regulated in response to ER stress, including homocysteine. Herp stabilizes neuronal Ca(2+) homeostasis and is involved in improving the ba...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.09.004

    authors: Lenz B,Bleich S,Beutler S,Schlierf B,Schwager K,Reulbach U,Kornhuber J,Bönsch D

    更新日期:2006-12-10 00:00:00

  • Variation and frequency of cytokeratin polypeptide patterns in human squamous non-keratinizing epithelium.

    abstract::The squamous non-keratinizing epithelium of the human upper digestive tract was analyzed for keratin-like cytoskeletal proteins (cytokeratins) by both high resolution one- and two-dimensional gel electrophoresis. The Triton/high salt-insoluble portion of pure epithelial homogenates contains a number of SDS- and urea-e...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90430-1

    authors: Wild GA,Mischke D

    更新日期:1986-01-01 00:00:00

  • The activated insulin-like growth factor I receptor induces depolarization in breast epithelial cells characterized by actin filament disassembly and tyrosine dephosphorylation of FAK, Cas, and paxillin.

    abstract::Insulin-like growth factor I (IGF-I) promotes the motility of different cell types. We investigated the role of IGF-I receptor (IGF-IR) signaling in locomotion of MCF-7 breast cancer epithelial cells overexpressing the wild-type IGF-IR (MCF-7/IGF-IR). Stimulation of MCF-7/IGF-IR cells with 50 ng/ml IGF-I induced disru...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4566

    authors: Guvakova MA,Surmacz E

    更新日期:1999-08-25 00:00:00

  • Nerve Growth Factor modulates LPS - induced microglial glycolysis and inflammatory responses.

    abstract::Microglia, the parenchymal immune cells of the central nervous system, orchestrate neuroinflammation in response to infection or damage, and promote tissue repair. However, aberrant microglial responses are integral to neurodegenerative diseases and critically contribute to disease progression. Thus, it is important t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.02.023

    authors: Fodelianaki G,Lansing F,Bhattarai P,Troullinaki M,Zeballos MA,Charalampopoulos I,Gravanis A,Mirtschink P,Chavakis T,Alexaki VI

    更新日期:2019-04-15 00:00:00

  • Possible involvement of the interaction of the alpha 5 subunit of alpha 5 beta 1 integrin with the synergistic region of the central cell-binding domain of fibronectin in cells to fibronectin binding.

    abstract::The interaction between the central cell-binding domain (CBD) of fibronectin (FN) and its receptor integrin alpha 5 beta 1 was analyzed by both ligand-binding and cell adhesion assays. The ligands used were a CBD fragment (pCBD) of human plasma fibronectin and its recombinant versions including wild type CBD (wtCBD) a...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1033

    authors: Obara M,Yoshizato K

    更新日期:1995-01-01 00:00:00

  • miR-203a is involved in HBx-induced inflammation by targeting Rap1a.

    abstract::Hepatitis B virus (HBV) causes acute and chronic hepatitis, and is one of the major causes of cirrhosis and hepatocellular carcinoma. Accumulating evidence suggests that inflammation is the key factor for liver cirrhosis and hepatocellular carcinoma. MicroRNAs play important roles in many biological processes. Here, w...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2016.10.016

    authors: Wu A,Chen H,Xu C,Zhou J,Chen S,Shi Y,Xu J,Gan J,Zhang J

    更新日期:2016-11-15 00:00:00

  • Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+.

    abstract::Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4071

    authors: Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

    更新日期:1998-07-10 00:00:00

  • Transmyocardial drilling revascularization combined with heparinized bFGF-incorporating stent activates resident cardiac stem cells via SDF-1/CXCR4 axis.

    abstract:OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.11.009

    authors: Zhang GW,Wen T,Gu TX,Li-Ling J,Wang C,Zhao Y,Liu J,Wang Y,Liu TJ,Lü F

    更新日期:2012-02-15 00:00:00

  • A functional role for ERK in gene induction, but not in neurite outgrowth in differentiating neuroblastoma cells.

    abstract::The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5163

    authors: Olsson AK,Nånberg E

    更新日期:2001-04-15 00:00:00

  • Rafoxanide promotes apoptosis and autophagy of gastric cancer cells by suppressing PI3K /Akt/mTOR pathway.

    abstract::Rafoxanide is commonly used as anti-helminthic medicine in veterinary medicine, a main compound of salicylanilide. Previous studies have reported that rafoxanide, as an inhibitor of BRAF V600E mutant protein, inhibits the growth of colorectal cancer, multiple myeloma, and skin cancer. However, its therapeutic effect o...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.111691

    authors: Liu JZ,Hu YL,Feng Y,Guo YB,Liu YF,Yang JL,Mao QS,Xue WJ

    更新日期:2019-12-15 00:00:00

  • A novel protein expressed in mammalian cells undergoing apoptosis.

    abstract::Human and rodent cells undergoing apoptosis were observed to express high levels of a novel 45,000 M(r) protein. The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of s...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1177

    authors: Grand RJ,Milner AE,Mustoe T,Johnson GD,Owen D,Grant ML,Gregory CD

    更新日期:1995-06-01 00:00:00

  • Regulation of subcellular localization of the antiproliferative protein Tob by its nuclear export signal and bipartite nuclear localization signal sequences.

    abstract::Tob, a member of the Tob and BTG antiproliferative protein family, plays an important role in many cellular processes including cell proliferation. In this study, we have addressed molecular mechanisms regulating subcellular localization of Tob. Treatment with leptomycin B, an inhibitor of nuclear export signal (NES) ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2003.12.016

    authors: Maekawa M,Yamamoto T,Nishida E

    更新日期:2004-04-15 00:00:00