Cortactin involvement in the keratinocyte growth factor and fibroblast growth factor 10 promotion of migration and cortical actin assembly in human keratinocytes.


:Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is not clearly established. Here we analyzed the motogenic activity of FGF10 and KGF on human keratinocytes. Migration assays and immunofluorescence of actin cytoskeleton revealed that FGF10 is less efficient than KGF in promoting migration and exerts a delayed effect in inducing lamellipodia and ruffles formation. Both growth factors promoted phosphorylation and subsequent membrane translocation of cortactin, an F-actin binding protein involved in cell migration; however, FGF10-induced cortactin phosphorylation was reduced, more transient and delayed with respect to that promoted by KGF. Cortactin phosphorylation induced by both growth factors was Src-dependent, while its membrane translocation and cell migration were blocked by either Src and PI3K inhibitors, suggesting that both pathways are involved in KGF- and FGF10-dependent motility. Furthermore, siRNA-mediated downregulation of cortactin inhibited KGF- and FGF10-induced migration. These results indicate that cortactin is involved in keratinocyte migration promoted by both KGF and FGF10.


Exp Cell Res


Ceccarelli S,Cardinali G,Aspite N,Picardo M,Marchese C,Torrisi MR,Mancini P




Has Abstract


2007-05-15 00:00:00














  • The IGF-1-IGF-1 receptor system modulates myocyte proliferation but not myocyte cellular hypertrophy in vitro.

    abstract::In preliminary experiments it was established that the hypertrophic and hyperplastic responses of neonatal cardiac myocytes in culture were associated with enhanced expression of IGF-1 and IGF-1 receptors in these cells. Therefore, to determine the role of IGF-1 receptors on myocyte growth, cells were exposed to antis...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kajstura J,Cheng W,Reiss K,Anversa P

    更新日期:1994-12-01 00:00:00

  • Specific binding of [3H]heparin to human carcinoma SW-13 and other mammalian cells.

    abstract::This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Halper J

    更新日期:1990-04-01 00:00:00

  • High glucose induces autophagy in podocytes.

    abstract::Autophagy is a cellular pathway involved in protein and organelle degradation. It is relevant to many types of cellular homeostasis and human diseases. High level of glucose is known to inflict podocyte injury, but little is reported about the relationship between high concentrations of glucose and autophagy in these ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ma T,Zhu J,Chen X,Zha D,Singhal PC,Ding G

    更新日期:2013-04-01 00:00:00

  • S100P/RAGE signaling regulates microRNA-155 expression via AP-1 activation in colon cancer.

    abstract::Accumulating evidence indicates that elevated S100P promotes the pathogenesis of cancers, including colon cancer. S100P exerts its effects by binding to and activating the Receptor for Advance Glycation End-products (RAGE). The effects of up-regulated S100P/RAGE signaling on cell functions are well documented. Despite...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Onyeagucha BC,Mercado-Pimentel ME,Hutchison J,Flemington EK,Nelson MA

    更新日期:2013-08-01 00:00:00

  • Ruta graveolens water extract inhibits cell-cell network formation in human umbilical endothelial cells via MEK-ERK1/2 pathway.

    abstract::Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gentile MT,Russo R,Pastorino O,Cioffi S,Barbieri F,Illingworth EA,Grieco M,Chambery A,Colucci-D'Amato L

    更新日期:2018-03-01 00:00:00

  • Subcellular location of a soluble factor that stimulates DNA replication in permeable animal cells.

    abstract::The subcellular location of a soluble DNA replication-stimulating factor released from lysolecithin-permeabilized cultured mammalian cells was investigated by cytochalasin B (CB) enucleation. In both Chinese hamster ovary cells and baby hamster kidney (BHK) cells, approx. 70% of the total activity of the replication-s...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Myers CA,Patel PI,Miller MR

    更新日期:1983-01-01 00:00:00

  • Vascular smooth muscle cell detachment from elastin and migration through elastic laminae is promoted by chondroitin sulfate-induced "shedding" of the 67-kDa cell surface elastin binding protein.

    abstract::Impaired elastin fiber assembly is observed in the fetal ductus arteriosus (DA), associated with a reduced concentration of elastin binding protein (EBP), a 67-kDa galactolectin. It is also seen in cultured aortic (Ao) smooth muscle cells (SMC) following the release of the EBP by glycosaminoglycans rich in N-acetylgal...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hinek A,Boyle J,Rabinovitch M

    更新日期:1992-12-01 00:00:00

  • Growth-related expression of a 72,000 molecular weight poly(A)+ mRNA binding protein.

    abstract::In this communication, we have studied a 72,000 mol w (p72) host protein which reacts with a mouse monoclonal antibody (PAb6) directed against antigenic determinants on the Simian virus 40 (SV40) large T antigen protein that map 5' of 0.42 map units on the viral genome. The p72 protein is an abundant basic (pI greater...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ullrich SJ,Appella E,Mercer WE

    更新日期:1988-10-01 00:00:00

  • Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus.

    abstract::Coiled bodies are conserved subnuclear organelles that contain splicing snRNPs, a subset of nucleolar antigens, and the autoantigen p80 coilin. Most nuclei contain one to five nucleoplasmic coiled bodies, often with one or more located at the nucleolar periphery. Here we show that exposure of mammalian cells to low le...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lyon CE,Bohmann K,Sleeman J,Lamond AI

    更新日期:1997-01-10 00:00:00

  • Hyaluronan production enhances shedding of plasma membrane-derived microvesicles.

    abstract::Many cell types secrete plasma membrane-bound microvesicles, suggested to play an important role in tissue morphogenesis, wound healing, and cancer spreading. However, the mechanisms of their formation have remained largely unknown. It was found that the tips of long microvilli induced in cells by overexpression of hy...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rilla K,Pasonen-Seppänen S,Deen AJ,Koistinen VVT,Wojciechowski S,Oikari S,Kärnä R,Bart G,Törrönen K,Tammi RH,Tammi MI

    更新日期:2013-08-01 00:00:00

  • Evolution of new proteins from translated sORFs in long non-coding RNAs.

    abstract::High throughput RNA sequencing techniques have revealed that a large fraction of the genome is transcribed into long non-coding RNAs (lncRNAs). Unlike canonical protein-coding genes, lncRNAs do not contain long open reading frames (ORFs) and tend to be poorly conserved across species. However, many of them contain sma...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Ruiz-Orera J,Villanueva-Cañas JL,Albà MM

    更新日期:2020-06-01 00:00:00

  • The extracellular matrix in breast cancer predicts prognosis through composition, splicing, and crosslinking.

    abstract::The extracellular matrix in the healthy breast has an important tumor suppressive role, whereas the abnormal ECM in tumors can promote aggressiveness, and has been linked to breast cancer relapse, survival and resistance to chemotherapy. This review article gives an overview of the elements of the ECM which have been ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Robertson C

    更新日期:2016-04-10 00:00:00

  • Both increased stability and transcription contribute to the induction of the urokinase plasminogen activator receptor (uPAR) message by hypoxia.

    abstract::Both hypoxia and overexpression of the urokinase plasminogen activator receptor (uPAR) are associated with a poor clinical outcome in human cancers. Hypoxia has been shown to induce uPAR expression in breast cancer cells and to increase their invasion through Matrigel, a phenomenon which can be blocked using an anti-u...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Maity A,Solomon D

    更新日期:2000-03-15 00:00:00

  • Novel aberrant genetic and epigenetic events in Friedreich's ataxia.

    abstract::It is generally accepted that Friedreich's ataxia (FRDA) is caused by a deficiency in frataxin expression, a mitochondrial protein involved in iron homeostasis, which mainly affects the brain, dorsal root ganglia of the spinal cord, heart and in certain cases the pancreas. However, there is little knowledge as to othe...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Quesada MP,Jones J,Rodríguez-Lozano FJ,Moraleda JM,Martinez S

    更新日期:2015-07-01 00:00:00

  • Characterization of glucocerebrosidase in peripheral blood cells and cultured blastoid cells.

    abstract::We have characterized glucocerebrosidase in various cell types of peripheral blood of control subjects and in cultured human blastoid cells. The intracellular level of glucocerebrosidase in cultured blastoid cells (10-30 nmol substrate hydrolyzed/ protein) resembles closely values observed for leukocyte cell types...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Aerts JM,Heikoop J,van Weely S,Donker-Koopman WE,Barranger JA,Tager JM,Schram AW

    更新日期:1988-08-01 00:00:00

  • Characterization of the intermediate filament proteins of murine mammary gland epithelial cells. Response to collagen substratum.

    abstract::The insoluble cytoskeletal material remaining after detergent lysis of 'Normal' Murine Mammary Gland (NMuMG) cells, growing on plastic or collagen gel substrata, was analyzed by two-dimensional gel electrophoresis. The identity of the cytoskeletal elements was determined by their solubility properties, electrophoretic...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hall HG,Bissell MJ

    更新日期:1986-02-01 00:00:00

  • siRNA - Mediated LRP/LR knock-down reduces cellular viability of malignant melanoma cells through the activation of apoptotic caspases.

    abstract::The 37 kDa/67 kDa laminin receptor (LRP/LR) is over-expressed in tumor cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rebelo TM,Vania L,Ferreira E,Weiss SFT

    更新日期:2018-07-01 00:00:00

  • pH in the endosome. Measurements during pinocytosis and receptor-mediated endocytosis.

    abstract::By fluorescence spectroscopy, the average pH within endocytic compartments was determined during endocytosis of fluorescein conjugates by macrophages and hepatocytes. In mouse macrophages and hepatocytes fluorescein conjugates taken up either in the fluid phase or by binding to cell surface receptors were rapidly tran...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Geisow MJ,Evans WH

    更新日期:1984-01-01 00:00:00

  • Developmental control of Presenilin1 expression, endoproteolysis, and interaction in zebrafish embryos.

    abstract::Dominant mutations in presenilin1 (PS1) and presenilin2 (PS2) are a major cause of early-onset Alzheimer's disease. In this report we analyze the expression of the zebrafish presenilin1 (Psen1) and presenilin2 (Psen2) proteins during embryogenesis. We demonstrate that Psen1 and Psen2 holoproteins are relatively abunda...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nornes S,Groth C,Camp E,Ey P,Lardelli M

    更新日期:2003-09-10 00:00:00

  • Identification of the type-B receptor for platelet-derived growth factor in human embryonal carcinoma cells.

    abstract::The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

    更新日期:1990-02-01 00:00:00

  • A-type nuclear lamins act as transcriptional repressors when targeted to promoters.

    abstract::Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lee DC,Welton KL,Smith ED,Kennedy BK

    更新日期:2009-04-01 00:00:00

  • Ceramide-induced apoptosis in fas-resistant Hodgkin's disease cell lines is caspase independent.

    abstract::We investigated whether cell-permeable, synthetic ceramide (C6 ceramide) could induce apoptosis in Fas-resistant Hodgkin's disease (HD)-derived cell lines. Despite strongly expressing the Fas-receptor, two of three HD-derived cell lines were resistant to Fas-mediated apoptosis. This resistance to Fas could not be attr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Metkar SS,Anand M,Manna PP,Naresh KN,Nadkarni JJ

    更新日期:2000-02-25 00:00:00

  • Expression of two different products of CDC25Mm, a mammalian Ras activator, during development of mouse brain.

    abstract::The CDC25Mm gene codes for Ras-guanine nucleotide exchange factors. Four different full-length cDNA clones derived from the same gene and coding for proteins of different sizes that have in common the last 661 amino acids have been isolated from mouse brain. In order to investigate the expression of the products of th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ferrari C,Zippel R,Martegani E,Gnesutta N,Carrera V,Sturani E

    更新日期:1994-02-01 00:00:00

  • Oxidative stress induced by palmitic acid modulates KCa2.3 channels in vascular endothelium.

    abstract::Elevated plasma free fatty acids level has been implicated in the development of insulin resistance, inflammation, and endothelial dysfunction in diabetic and nondiabetic individuals. However, the underlying mechanisms still remain to be defined. Herein, we investigated the effect of palmitic acid (PA), the most abund...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wang Y,Wang XJ,Zhao LM,Pang ZD,She G,Song Z,Cheng X,Du XJ,Deng XL

    更新日期:2019-10-15 00:00:00

  • Identification of avian sarcoplasmic reticulum Ca(2+)-ATPase (SERCA3) as a novel 1,25(OH)(2)D(3) target gene in the monocytic lineage.

    abstract::Osteoclasts are postmitotic, multinucleated giant cells generated by the fusion of hematopoietic mononuclear precursors from the monocyte-macrophage lineage. In culture, adherent macrophages from blood-derived monocytes grow, gather, and fuse together to form multinucleated osteoclast-like cells. These events are cont...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Machuca I,Domenget C,Jurdic P

    更新日期:1999-08-01 00:00:00

  • Epidermal growth factor and transforming growth factor-beta1 enhance HK-2 cell migration through a synergistic increase of matrix metalloproteinase and sustained activation of ERK signaling pathway.

    abstract::Epidermal growth factor (EGF) and transforming growth factor-beta1 (TGF-beta1), upregulated in renal diseases, have a combinational effect on epithelial-mesenchymal transformation (EMT) of renal proximal tubular cells. The aim of this study was to examine the mechanism regarding the combinational effect of EGF and TGF...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tian YC,Chen YC,Chang CT,Hung CC,Wu MS,Phillips A,Yang CW

    更新日期:2007-07-01 00:00:00

  • Recent progress in protocadherin research.

    abstract::Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Suzuki ST

    更新日期:2000-11-25 00:00:00

  • Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody.

    abstract::A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schmidt-Zachmann MS,Hügle B,Scheer U,Franke WW

    更新日期:1984-08-01 00:00:00

  • Acute myocardial infarction leads to upregulation of the IGF-1 autocrine system, DNA replication, and nuclear mitotic division in the remaining viable cardiac myocytes.

    abstract::Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

    更新日期:1994-08-01 00:00:00

  • Abnormal behavior of the yolk centrosomes during early embryogenesis of Drosophila melanogaster.

    abstract::After the 10th nuclear cycle the yolk centrosomes follow an irregular pathway. Unlike the somatic centrosomes, which move to the opposite poles of the nuclei to form the bipolar spindles, the yolk centrosomes remain as pairs at one pole of the yolk nuclei or shift feebly and nucleate irregular spindles, most of which ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Callaini G,Dallai R

    更新日期:1991-01-01 00:00:00