Expression of proliferating cell nuclear antigen in migrating retinal pigment epithelial cells during wound healing in organ culture.

abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...

journal_title：Experimental cell research

authors： Nakamura H,Morita T,Sato C

更新日期：1986-08-01 00:00:00

abstract：:When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta and vitamin D can influence this process and can stimulate chondrogenesis. Given the ...

journal_title：Experimental cell research

authors： Tsonis PA,Del Rio-Tsonis K,Millan JL,Wheelock MJ

更新日期：1994-08-01 00:00:00

abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

journal_title：Experimental cell research

authors： Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

更新日期：2006-01-01 00:00:00

abstract：:Human endothelial cells, when suspended within three-dimensional collagen matrices, develop intracellular vacuoles that coalesce to form capillary lumens and tubes. Vacuole and lumen formation are completely dependent on the collagen-binding integrin alpha 2 beta 1, while other endothelial cell integrins had no appare...

journal_title：Experimental cell research

authors： Davis GE,Camarillo CW

更新日期：1996-04-10 00:00:00

abstract：:Bax-mediated apoptosis in neurons is involved in many pathologic conditions affecting the central nervous system, including degenerative diseases, stroke, and trauma. Two molecules belonging to the Bcl-2 family, Bcl-2 and Bcl-X(L), protect cells from Bax-induced apoptosis and show distinct expression patterns in adult...

journal_title：Experimental cell research

authors： Shinoura N,Satou R,Yoshida Y,Asai A,Kirino T,Hamada H

更新日期：2000-02-01 00:00:00

abstract：:In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...

journal_title：Experimental cell research

authors： Valmassoi J,Schwarz RI

更新日期：1988-06-01 00:00:00

abstract：:The effect of 1 microM antimycin on the proliferative properties, metabolism and basic cell composition of Ehrlich ascites tumour cells cultured in the second in vitro passage was studied. Continuous drug exposure of asynchronous cells caused rapid cessation of cell growth, characterized by the cell number and DNA, RN...

journal_title：Experimental cell research

authors： Löffler M

更新日期：1985-03-01 00:00:00

abstract：:Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed ...

journal_title：Experimental cell research

authors： Lönnroth A,Alexciev K,Mehlin H,Wurtz T,Skoglund U,Daneholt B

更新日期：1992-04-01 00:00:00

abstract：:Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...

journal_title：Experimental cell research

authors： Lee C,Dixelius J,Thulin A,Kawamura H,Claesson-Welsh L,Olsson AK

更新日期：2006-08-01 00:00:00

abstract：:The process of tumor cell invasion of the basement membrane is proposed to consist of three steps: attachment, local proteolysis and migration. 12-(S)-HETE, a 12-lipoxygenase metabolite of arachidonic acid, upregulates surface expression of integrin cytoadhesins and an autocrine motility factor receptor, suggesting th...

journal_title：Experimental cell research

authors： Honn KV,Timár J,Rozhin J,Bazaz R,Sameni M,Ziegler G,Sloane BF

更新日期：1994-09-01 00:00:00

abstract：:The results of Phase I/II clinical trials indicate that ricin A-chain-containing immunotoxins cause vascular leak syndrome, characterized by hypoalbuminemia with resultant weight gain and edema. Vascular leak syndrome may be a dose-limiting factor during treatment with ricin A-chain-containing immunotoxins. In this re...

journal_title：Experimental cell research

authors： Soler-Rodríguez AM,Ghetie MA,Oppenheimer-Marks N,Uhr JW,Vitetta ES

更新日期：1993-06-01 00:00:00

abstract：:The sensitivity to serine proteinases of cellular proteins involved in cell-matrix adhesion was investigated using C32 melanoma cells. Cells dissociated from monolayers by the metal chelator ethylenediaminetetraacetic acid were incubated with proteolytic enzymes, and then attachment was quantified by standard cell adh...

journal_title：Experimental cell research

authors： Fujii K,Lazarus GS,Schechter NM

更新日期：1993-09-01 00:00:00

abstract：:Osteoporosis due to unloading-induced bone loss is a critical issue in the modern aging society. Although the mechanisms underlying this phenomenon are largely unknown, osteopontin (OPN) is one of the critical mediators required for unloading-induced bone loss [M. Ishijima, S.R. Rittling, T. Yamashita, K. Tsuji, H. Ku...

journal_title：Experimental cell research

authors： Ishijima M,Ezura Y,Tsuji K,Rittling SR,Kurosawa H,Denhardt DT,Emi M,Nifuji A,Noda M

更新日期：2006-10-01 00:00:00

abstract：:Breast cancer (BC) is one of the most common cancers among women in both developed and developing countries with a rising incidence. Using the MMTV-PyMT transgenic mouse model and xenografted breast cancer model, we found that R5, a neutralizing antibody to Robo1, significantly inhibited BC growth and metastasis. Angi...

journal_title：Experimental cell research

authors： Li Q,Cao J,He Y,Liu X,Mao G,Wei B,Liao S,Zhang Q,Li J,Zheng L,Wang L,Qi C

更新日期：2020-02-01 00:00:00

abstract：:In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RII alpha RII beta of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RII alpha and RII beta show that these ...

journal_title：Experimental cell research

authors： Keryer G,Rios RM,Landmark BF,Skalhegg B,Lohmann SM,Bornens M

更新日期：1993-02-01 00:00:00

abstract：:T51B rat liver epithelial cells were stimulated with extracellular ATP. Changes in cytoplasmic free Ca2+ concentration [( Ca2+]i) were measured by fura-2 both in a large population of cells on coverslips in a cuvette and in single cells in a microscopic system. Extracellular ATP evoked a prompt increase in [Ca2+]i in ...

journal_title：Experimental cell research

authors： Boynton AL,Cooney RV,Hill TD,Nilsson T,Arkhammar P,Berggren PO

更新日期：1989-03-01 00:00:00

abstract：:The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation an...

journal_title：Experimental cell research

authors： Rahe MC,Dvorak CMT,Wiseman B,Martin D,Murtaugh MP

更新日期：2020-05-15 00:00:00

abstract：:Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

journal_title：Experimental cell research

authors： De Wever B,Rooman RP,De Brabander M

更新日期：1990-01-01 00:00:00

abstract：:We report here the first direct observation of chemotaxis to EGF by rat mammary carcinoma cells. When exposed to a gradient of EGF diffusing from a micropipette, MTLn3 cells displayed typical ameboid chemotaxis, extending a lamellipod-like protrusion and moving toward the pipette. Using a homogeneous upshift in EGF to...

journal_title：Experimental cell research

authors： Bailly M,Yan L,Whitesides GM,Condeelis JS,Segall JE

更新日期：1998-06-15 00:00:00

abstract：:The large zinc finger protein KRC regulates transcription of target genes via the kappaB gene enhancer element. As an attempt to investigate the cellular function of KRC, we have established cell lines stably transfected with KRC expression vectors. Introduction of a vector directing expression of a transcript antisen...

journal_title：Experimental cell research

authors： Allen CE,Wu LC

更新日期：2000-11-01 00:00:00

abstract：:The formation of functional gap junctions between astrocytes was investigated during differentiation of these cells in culture. Precursor cells of GFA (glial fibrillary acidic) protein-positive astrocytes were cultured in a chemically defined medium as a homogeneous population. These cells were rarely coupled to one n...

journal_title：Experimental cell research

authors： Fischer G,Kettenmann H

更新日期：1985-08-01 00:00:00

abstract：:Previous studies have shown that ascorbic acid increases both the total surface acetylcholine receptor (AChR) expression and the mRNA for the alpha-subunit of this receptor in myotubes of cloned L5 muscle cultures. Since ascorbic acid increases collagen synthesis in fibroblasts, we studied the effect of ascorbic acid ...

journal_title：Experimental cell research

authors： Liu E,Minor RR,Horovitz O,Wootton JA,Podleski TR,Salpeter MM

更新日期：1993-11-01 00:00:00

abstract：:Pluripotent stem cell activity is essential to maintain regeneration and homeostasis in the Drosophila midgut following environmental challenges. Although multiple pathways have been implicated in epithelial renewal, the underlying regulatory mechanisms and correlations between relevant genes and pathways remain elusi...

journal_title：Experimental cell research

authors： Liu Q,Jin LH

更新日期：2017-12-15 00:00:00

abstract：:On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

journal_title：Experimental cell research

authors： Ohno T,Kako R,Sato C,Ohkawa A

更新日期：1986-04-01 00:00:00

abstract：:After the 10th nuclear cycle the yolk centrosomes follow an irregular pathway. Unlike the somatic centrosomes, which move to the opposite poles of the nuclei to form the bipolar spindles, the yolk centrosomes remain as pairs at one pole of the yolk nuclei or shift feebly and nucleate irregular spindles, most of which ...

journal_title：Experimental cell research

authors： Callaini G,Dallai R

更新日期：1991-01-01 00:00:00

abstract：:Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in gr...

journal_title：Experimental cell research

authors： Kondo H,Ide H

更新日期：1983-11-01 00:00:00

abstract：:Hypoxia inducible factor 1 (HIF-1), the key mediator of hypoxia signaling pathways, has been shown involved in hypoxia-induced radioresistance. However, the underlying mechanisms are unclear. The present study demonstrated that both hypoxia and hypoxia mimetic cobalt chloride could increase the radioresistance of huma...

journal_title：Experimental cell research

authors： Liu J,Zhang J,Wang X,Li Y,Chen Y,Li K,Zhang J,Yao L,Guo G

更新日期：2010-07-15 00:00:00

abstract：:We have earlier shown that mouse pancreatic acinar cells produce hydrogen sulfide (H(2)S), which plays a key role in the pathogenesis of acute pancreatitis (AP). H(2)S-dependent induction of inflammation is mediated by the activation of transcription factor NF-kappaB. We now provide evidence that activation of Src fam...

journal_title：Experimental cell research

authors： Tamizhselvi R,Koh YH,Sun J,Zhang H,Bhatia M

更新日期：2010-05-15 00:00:00

abstract：:The cellular targets for the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, are unknown. Other studies have indicated that the mature form of p21 is distributed diffusely on the cytoplasmic face of the plasma membrane. However, after fixation without buffer washes, indirect immunofluorescent staini...

journal_title：Experimental cell research

authors： Myrdal SE,Auersperg N

更新日期：1985-08-01 00:00:00

abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

journal_title：Experimental cell research

authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

更新日期：1996-12-15 00:00:00