当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Hydrogen sulfide induces ICAM-1 expression and neutrophil adhesion to caerulein-treated pancreatic acinar cells through NF-kappaB and Src-family kinases pathway.

Hydrogen sulfide induces ICAM-1 expression and neutrophil adhesion to caerulein-treated pancreatic acinar cells through NF-kappaB and Src-family kinases pathway.

Abstract：

:We have earlier shown that mouse pancreatic acinar cells produce hydrogen sulfide (H(2)S), which plays a key role in the pathogenesis of acute pancreatitis (AP). H(2)S-dependent induction of inflammation is mediated by the activation of transcription factor NF-kappaB. We now provide evidence that activation of Src family kinases (SFKs) is crucial in signaling H(2)S-induced intracellular adhesion molecule (ICAM)-1 expression via NF-kappaB. Stimulation of acini with H(2)S resulted in a time-dependent activation of SFKs. In order to better understand this effect of H(2)S, acinar cells were stimulated with caerulein after addition of H(2)S donor, NaHS. Inhibition of SFKs impaired H(2)S-induced NF-kappaB activity and ICAM-1 expression in caerulein treated acinar cells. We also observed that H(2)S-induced up-regulation of ICAM-1 enhanced the adhesion of neutrophils onto acinar cells. Analysis of NF-kappaB pathway revealed that the effect of SFKs inhibition correlated with IkappaBalpha degradation and NF-kappaB DNA binding function. Interestingly, H(2)S-induced association of SFKs with translocation of NF-kappaB, and inhibition of SFKs prevented this response, indicating that this interaction may depend on activation of SFKs. These data suggest that H(2)S, by activating the phosphorylation of SFKs, may promote the transcriptional activity of NF-kappaB and eventually lead to an upregulation of ICAM-1 expression.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Tamizhselvi R,Koh YH,Sun J,Zhang H,Bhatia M

doi

10.1016/j.yexcr.2010.02.044

subject

Has Abstract

pub_date

2010-05-15 00:00:00

pages

1625-36

issue

9

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(10)00106-0

journal_volume

316

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Poly(ADP ribose) polymerase-defective mutant cell clone of mouse L1210 cells.

    abstract：:By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 2...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(05)80080-1

    authors： Yoshihara K,Itaya A,Hironaka T,Sakuramoto S,Tanaka Y,Tsuyuki M,Inada Y,Kamiya T,Ohnishi K,Honma M

    更新日期：1992-05-01 00:00:00

  • Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes.

    abstract：:The calcium/calmodulin-dependent protein phosphatase calcineurin is required for the induction of transcriptional events that initiate and promote myogenic differentiation. An important effector for calcineurin in striated muscle is the transcription factor myocyte enhancer factor 2 (MEF2). The targeting of the enzyme...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.12.016

    authors： Li J,Vargas MA,Kapiloff MS,Dodge-Kafka KL

    更新日期：2013-02-15 00:00:00

  • Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.

    abstract：:Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.12.019

    authors： Oikawa Y,Hansson J,Sasaki T,Rousselle P,Domogatskaya A,Rodin S,Tryggvason K,Patarroyo M

    更新日期：2011-05-01 00:00:00

  • Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway.

    abstract：:The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4586

    authors： Sakkas D,Mariethoz E,St John JC

    更新日期：1999-09-15 00:00:00

  • Gp38k, a protein synthesized by vascular smooth muscle cells, stimulates directional migration of human umbilical vein endothelial cells.

    abstract：:Gp38k is a 383-amino-acid secreted glycoprotein expressed by cultured vascular smooth muscle cells during the time of transition from a proliferating monolayer culture to a nonproliferating multilayered (differentiated) culture. Expression continues as the cell culture forms multicellular nodules. Because this transit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4511

    authors： Malinda KM,Ponce L,Kleinman HK,Shackelton LM,Millis AJ

    更新日期：1999-07-10 00:00:00

  • p53 Induces myocyte apoptosis via the activation of the renin-angiotensin system.

    abstract：:The mechanism by which p53 activates apoptosis in various cell systems is unknown. In the absence of an external death stimulus, p53 and p53-dependent genes, bcl-2 and bax, cannot trigger apoptosis. However, p53 may enhance not only transcription of bax and repress bcl-2, but also may upregulate the local renin-angiot...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3604

    authors： Pierzchalski P,Reiss K,Cheng W,Cirielli C,Kajstura J,Nitahara JA,Rizk M,Capogrossi MC,Anversa P

    更新日期：1997-07-10 00:00:00

  • Phenotype-dependent expression of alpha-smooth muscle actin in visceral smooth muscle cells.

    abstract：:Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4339

    authors： Saga H,Kimura K,Hayashi K,Gotow T,Uchiyama Y,Momiyama T,Tadokoro S,Kawashima N,Jimbou A,Sobue K

    更新日期：1999-02-25 00:00:00

  • Microinjected anti-actin antibodies decrease gap junctional intercellular commmunication in cultured astrocytes.

    abstract：:The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5652

    authors： Theiss C,Meller K

    更新日期：2002-12-10 00:00:00

  • Mononuclear cell-mediated modulation of synovial cell metabolism. I. Collagen synthesis.

    abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90431-8

    authors： Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

    更新日期：1985-05-01 00:00:00

  • Laminin-induced process outgrowth from isolated fetal rat C-cells.

    abstract：:A method for isolation of C-cells from rat fetuses was developed, and the morphological plasticity of the cells in primary culture systems was tested. Thyroid-parathyroid-ultimobranchial body (UB) complexes from 16-day rat fetuses were treated with 0.1% collagenase and 1000 PU/ml Dispase at 37 degrees C for 1 h. After...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90373-g

    authors： Nishiyama I,Fujii T

    更新日期：1992-02-01 00:00:00

  • Interaction of methylmercury with microtubules in cultured cells and in vitro.

    abstract：:The effects of methylmercury (MeHg) on cytoplasmic microtubules in cultured fibroblasts and on the in vitro polymerization of microtubules were examined. MeHg caused disruption of cellular microtubules in a concentration- and time-dependent manner. Addition of the metal-chelating agent, dimercaptosuccinic acid (DMSA),...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90331-2

    authors： Sager PR,Doherty RA,Olmsted JB

    更新日期：1983-06-01 00:00:00

  • In situ hybridization in living cells: detection of RNA molecules.

    abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3464

    authors： Paillasson S,Van De Corput M,Dirks RW,Tanke HJ,Robert-Nicoud M,Ronot X

    更新日期：1997-02-25 00:00:00

  • The beta 4 integrin subunit is expressed in mouse fibroblasts and modulated by transforming growth factor-beta 1.

    abstract：:Integrin beta 4 subunit is present in association with alpha 6 chain on both normal and transformed epithelial cells. Recently alpha 6 beta 4 heterodimer was found on the endothelium of medium-sized blood vessels and on immature thymocytes. In this report we show, by Northern blotting, indirect immunofluorescence, imm...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0271

    authors： Scardigli R,Soddu S,Falcioni R,Crescenzi M,Cimino L,Sacchi A

    更新日期：1996-09-15 00:00:00

  • Annexin VI is a mannose-6-phosphate-independent endocytic receptor for bovine β-glucuronidase.

    abstract：:Endocytosis and transport of bovine liver β-glucuronidase to lysosomes in human fibroblasts are mediated by two receptors: the well-characterized cation-independent mannose 6-phosphate receptor (IGF-II/Man6PR) and an IGF-II/Man6PR-independent receptor, which recognizes a Ser-Trp*-Ser sequence present on the ligand. Th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.05.025

    authors： Ramírez-Mata A,Michalak C,Mendoza-Hernández G,León-Del-Río A,González-Noriega A

    更新日期：2011-10-01 00:00:00

  • Secretory phospholipase A(2) inhibits epidermal growth factor-induced receptor activation.

    abstract：:Secretory phospholipase A(2) (sPLA(2)) plays important roles in mediating various cellular processes, including cell proliferation, differentiation, apoptosis, and inflammatory response. In this study, we demonstrated that a basic sPLA(2) inhibits epidermal growth factor (EGF)-induced EGF receptor activation, as deter...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5622

    authors： Zhao S,Du XY,Chen JS,Zhou YC,Song JG

    更新日期：2002-10-01 00:00:00

  • Nuclear localization of Lyn tyrosine kinase mediated by inhibition of its kinase activity.

    abstract：:Src-family kinases, cytoplasmic enzymes that participate in various signaling events, are found at not only the plasma membrane but also subcellular compartments, such as the nucleus, the Golgi apparatus and late endosomes/lysosomes. Lyn, a member of the Src-family kinases, is known to play a role in DNA damage respon...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.08.019

    authors： Ikeda K,Nakayama Y,Togashi Y,Obata Y,Kuga T,Kasahara K,Fukumoto Y,Yamaguchi N

    更新日期：2008-11-01 00:00:00

  • Upregulated SCUBE2 expression in breast cancer stem cells enhances triple negative breast cancer aggression through modulation of notch signaling and epithelial-to-mesenchymal transition.

    abstract：BACKGROUND:Metastatic and/or recurrent breast carcinomas are leading causes of cancer-related death worldwide. Breast cancer stem cells (BCSCs) have been implicated in cancer metastases and progression, thus, the need for the discovery and development of effective BCSCs-specific therapies against metastatic and triple ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.07.008

    authors： Chen JH,Kuo KT,Bamodu OA,Lin YC,Yang RB,Yeh CT,Chao TY

    更新日期：2018-09-15 00:00:00

  • Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle.

    abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0382

    authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

    更新日期：1996-12-15 00:00:00

  • Occurrence of proteinaceous 10-nm filaments throughout the cytoplasm of algae of the order Dasycladales.

    abstract：:Previously, whole-mount electron microscopy of nuclei extruded together with residual cytoplasm from the rhizoids of several algal species of the order Dasycladales has revealed the occurrence of an intra- and perinuclear network of 10-nm filaments morphologically indistinguishable from that of mammalian vimentin inte...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3924

    authors： Berger S,Wittke W,Traub P

    更新日期：1998-05-01 00:00:00

  • Tumor suppression by chromosome 11 is not due to cellular senescence.

    abstract：:Previous hybrid studies involving fusion of normal with immortal human cells indicated that the phenotype of cellular senescence is dominant and that immortality results from recessive changes in normal growth regulatory genes. We have further assigned 28 different immortal human cell lines to at least four complement...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90179-x

    authors： Ning Y,Shay JW,Lovell M,Taylor L,Ledbetter DH,Pereira-Smith OM

    更新日期：1991-01-01 00:00:00

  • Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.

    abstract：:Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.06.012

    authors： Crimaldi L,Courtneidge SA,Gimona M

    更新日期：2009-09-10 00:00:00

  • The Drosophila DOCK family protein sponge is involved in differentiation of R7 photoreceptor cells.

    abstract：:The Drosophila sponge (spg)/CG31048 gene belongs to the dedicator of cytokinesis (DOCK) family genes that are conserved in a wide variety of species. DOCK family members are known as DOCK1-DOCK11 in mammals. Although DOCK1 and DOCK2 involve neurite elongation and immunocyte differentiation, respectively, the functions...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.024

    authors： Eguchi K,Yoshioka Y,Yoshida H,Morishita K,Miyata S,Hiai H,Yamaguchi M

    更新日期：2013-08-15 00:00:00

  • Serelaxin inhibits differentiation and fibrotic behaviors of cardiac fibroblasts by suppressing ALK-5/Smad2/3 signaling pathway.

    abstract：:Serelaxin, a recombinant form of human relaxin-2, is currently regarded as a novel drug for treatment of acute heart failure. However, whether therapeutic effects of serelaxin are achieved by inhibiting cardiac fibrosis remains unclear. In this study, we investigate effects of serelaxin on inhibiting cardiac fibrosis....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.10.004

    authors： Wu XP,Wang HJ,Wang YL,Shen HR,Tan YZ

    更新日期：2018-01-01 00:00:00

  • Nuclear actin filaments and their topological changes in frog oocytes.

    abstract：:Previous morphological and biochemical studies have suggested that actin and actin-containing filaments (microfilaments) exist in the eukaryotic nucleus and that they perform important nuclear functions. However, the concept is not widely accepted. In this study, we demonstrate actin and bundles of actin in the nuclei...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1101

    authors： Parfenov VN,Davis DS,Pochukalina GN,Sample CE,Bugaeva EA,Murti KG

    更新日期：1995-04-01 00:00:00

  • Secreted collagen induced by ascorbic acid in L5 cloned muscle cultures does not affect acetylcholine receptor expression.

    abstract：:Previous studies have shown that ascorbic acid increases both the total surface acetylcholine receptor (AChR) expression and the mRNA for the alpha-subunit of this receptor in myotubes of cloned L5 muscle cultures. Since ascorbic acid increases collagen synthesis in fibroblasts, we studied the effect of ascorbic acid ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1287

    authors： Liu E,Minor RR,Horovitz O,Wootton JA,Podleski TR,Salpeter MM

    更新日期：1993-11-01 00:00:00

  • EYA1 promotes tumor angiogenesis by activating the PI3K pathway in colorectal cancer.

    abstract：:Blood vessels are one of the major routes for the dissemination of cancer cells. Malignant tumors release growth factors such as vascular endothelial growth factor(VEGF) to induce angiogenesis, thereby promoting metastasis. Here, we report that The Drosophila Eyes Absent Homologue 1 (EYA1), which is overexpressed in c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.02.028

    authors： Cai S,Cheng X,Liu Y,Lin Z,Zeng W,Yang C,Liu L,Chukwuebuka OA,Li W

    更新日期：2018-06-01 00:00:00

  • Unidirectional co-stimulation by a non-mating strain of Tetrahymena thermophila.

    abstract：:We report that a fatty acid auxotroph of Tetrahymena thermophila (RH179E1) fails to mate, yet retains the ability to co-stimulate normal cells unidirectionally. Thus, co-stimulation can be analyzed experimentally in the absence of pair formation. We show that the co-stimulation of normal cells of one mating type is su...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90225-4

    authors： Katz M,Conner RL,Baum MP,Orias E

    更新日期：1987-03-01 00:00:00

  • Construction of transferrin-coated liposomes for in vivo transport of exogenous DNA to bone marrow erythroblasts in rabbits.

    abstract：:A method for coating liposomes with transferrin is described. Such liposomes have been used for carrying exogenous DNA to rabbit bone marrow (BM) precursor cells, in vivo. A large amount of the DNA contained in the liposomes was delivered specifically into the erythroblasts of the test animals. ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90056-x

    authors： Stavridis JC,Deliconstantinos G,Psallidopoulos MC,Armenakas NA,Hadjiminas DJ,Hadjiminas J

    更新日期：1986-06-01 00:00:00

  • Participation of the NG2 proteoglycan in rat aortic smooth muscle cell responses to platelet-derived growth factor.

    abstract：:Through immunohistochemical studies we have identified the cell-surface proteoglycan, NG2, on blood vessels throughout the rat embryo. The particular cell type expressing this chondroitin sulfate proteoglycan, however, is dependent upon tissue location. Microvessels within the rat CNS express NG2 on endothelial cells,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1371

    authors： Grako KA,Stallcup WB

    更新日期：1995-11-01 00:00:00

  • Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition.

    abstract：:Integrin-linked kinase (ILK) is a multifunctional serine/threonine kinase. Accumulating evidences suggest that ILK are involved in cell-matrix interactions, cell proliferation, invasion, migration, angiogenesis and Epithelial-mesenchymal transition (EMT). However, the underlying mechanisms remain largely unknown. EMT ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.014

    authors： Xing Y,Qi J,Deng S,Wang C,Zhang L,Chen J

    更新日期：2013-08-01 00:00:00