Abstract:
:Gp38k is a 383-amino-acid secreted glycoprotein expressed by cultured vascular smooth muscle cells during the time of transition from a proliferating monolayer culture to a nonproliferating multilayered (differentiated) culture. Expression continues as the cell culture forms multicellular nodules. Because this transition period involves active cell migration, we evaluated the effects of exogenously added gp38k on vascular endothelial cell (HUVEC) migration and chemotaxis. Here we demonstrate that gp38k acts as a chemoattractant for HUVECs and stimulates cell migration in Boyden chambers at a level comparable to that achieved with the known endothelial cell chemoattractant bFGF. The migration effect is neutralized by the presence of a polyclonal anti-gp38k antibody. Because gp38k expression is also correlated with changes in culture morphology, we also assessed its ability to act as an agonist of HUVEC morphology using cultures growing on Matrigel. We report that gp38k stimulates endothelial cell tubulogenesis in this assay system. These results provide the first evidence that gp38k may function in angiogenesis by stimulating the migration and reorganization of vascular endothelial cells.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Malinda KM,Ponce L,Kleinman HK,Shackelton LM,Millis AJdoi
10.1006/excr.1999.4511keywords:
subject
Has Abstractpub_date
1999-07-10 00:00:00pages
168-73issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(99)94511-1journal_volume
250pub_type
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