Abstract：
:Phorbol esters have been reported to induce opposite responses in fetal myoblasts and in satellite cells isolated from adult skeletal muscles. We examined the possibility that different levels of protein kinase C (PKC) activity and different phorbol ester binding characteristics account for these responses. For this purpose, the subcellular distributions of PKC were compared in primary cultures of myogenic cells from fetal and adult rat muscles and in the L6 cell line. Cells were used at the proliferative stage or after differentiation into myotubes. Binding of phorbol dibutyrate (PDBu) was assayed. In all three cell types, the levels of PKC specific activity were comparable at the proliferating and the differentiated stages, and partial translocation of PKC activity from the membrane to the cytosolic compartment was observed after differentiation into myotubes. PDBu binding, which had a Kd of 6 to 13 nM in proliferative cells, rose to between 30 and 52 nM in myotubes. Simultaneously, a small increase was observed in the total number of PDBu binding sites. These results suggest that the role of PKC might change with the stage of differentiation. They also imply that the difference described by others between the sensitivity to phorbol esters of fetal myoblasts and satellite cells is not connected with the phorbol ester receptor (i.e., PKC), but might be caused by events subsequent to PKC activation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Martelly I,Gautron J,Moraczewski Jdoi
10.1016/0014-4827(89)90420-5subject
Has Abstractpub_date
1989-07-01 00:00:00pages
92-100issue
1eissn
0014-4827issn
1090-2422journal_volume
183pub_type
杂志文章abstract：:In DNA isolated from proliferating human lymphoblastoid CCRF-CEM cells which had been pulse-labeled by exposure to [3H]thymidine for periods from 30 s to 10 min, single-stranded regions were analyzed by caffeine-gradient elution from benzoylated DEAE-cellulose. Two classes of structural defect were evident. Some repli...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90087-b
更新日期：1991-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90198-5
更新日期：1989-03-01 00:00:00
abstract：:The signals generated by the IFNgamma receptor to initiate mRNA translation and generation of protein products that mediate IFNgamma responses are largely unknown. In the present study, we provide evidence for the existence of an IFNgamma-dependent signaling cascade activated downstream of the phosphatidylinositol (PI...
journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90405-9
更新日期：1989-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5052
更新日期：2000-11-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1997-08-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1988-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1150
更新日期：1993-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90421-4
更新日期：1983-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(02)00036-8
更新日期：2003-04-01 00:00:00
abstract：:Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.01.009
更新日期：2006-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(02)00056-3
更新日期：2003-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1114
更新日期：1995-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4446
更新日期：1999-06-15 00:00:00
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doi：10.1016/j.yexcr.2018.03.009
更新日期：2018-05-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.05.008
更新日期：2013-07-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.08.007
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.01.011
更新日期：2004-05-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2017-02-01 00:00:00
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更新日期：2018-10-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.09.010
更新日期：2004-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.3364
更新日期：1997-01-10 00:00:00
abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2012.02.026
更新日期：2012-05-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112337
更新日期：2020-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.01.012
更新日期：2012-03-10 00:00:00
abstract：:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4681
更新日期：1999-12-15 00:00:00
abstract：:In confluent keratinocyte monolayers, desmosomal adhesion gradually becomes calcium-independent and this is associated with an increase in the strength of intercellular adhesion (hyper-adhesion). In this study, we investigated the functional and molecular significance of hyper-adhesion in a system challenged by autoim...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.10.005
更新日期：2010-02-15 00:00:00
