An alpha 2 beta 1 integrin-dependent pinocytic mechanism involving intracellular vacuole formation and coalescence regulates capillary lumen and tube formation in three-dimensional collagen matrix.


:Human endothelial cells, when suspended within three-dimensional collagen matrices, develop intracellular vacuoles that coalesce to form capillary lumens and tubes. Vacuole and lumen formation are completely dependent on the collagen-binding integrin alpha 2 beta 1, while other endothelial cell integrins had no apparent influence. Vacuole formation occurs by a pinocytic process with internalization of plasma membrane and molecules from the extracellular space, such as fluorescent tracers. By immunofluorescence, vacuole membranes were found to contain associated cell surface proteins, proteins involved in endosomal trafficking (i.e., caveolin and annexin II), and F-actin. Furthermore, some vacuole compartments contained von Willebrand factor. Integrin-regulated vacuole formation and coalescence are major mechanisms controlling capillary lumen and tube formation within a three-dimensional extracellular matrix.


Exp Cell Res


Davis GE,Camarillo CW




Has Abstract


1996-04-10 00:00:00














  • Two distinct steps of Bak regulation during apoptotic stress signaling: different roles of MEKK1 and JNK1.

    abstract::Stress-activated protein (SAP) kinases and the mitochondrial pro-apoptotic Bcl-2 protein Bak are important regulators of apoptosis. Reduced expression of Bak increases cellular resistance to the anticancer agent cisplatin, and we report here that mouse embryo fibroblasts deficient in the SAP kinase jnk1 are highly res...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ihrlund LS,Hernlund E,Viktorsson K,Panaretakis T,Barna G,Sabapathy K,Linder S,Shoshan MC

    更新日期:2006-05-15 00:00:00

  • Glorin-regulated protein synthesis in Polyspondylium violaceum.

    abstract::Protein synthesis by Polyspondylium violaceum in response to the chemoattractant glorin was analyzed by two-dimensional gel electrophoresis to determine if glorin can affect gene expression. When cells developing in a shaking suspension culture were given glorin, five proteins exhibited significantly increased and thr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kopachik W

    更新日期:1990-02-01 00:00:00

  • Domain analysis of the actin-binding and actin-remodeling activities of drebrin.

    abstract::Drebrin is an actin-binding protein which is expressed at highly levels in neurons. When introduced into fibroblasts, it has been known to bind to F-actin and to cause remodeling of F-actin. Here, we performed a domain analysis of the actin-binding and actin-remodeling activities of drebrin. Various fragments of drebr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hayashi K,Ishikawa R,Kawai-Hirai R,Takagi T,Taketomi A,Shirao T

    更新日期:1999-12-15 00:00:00

  • Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1 proteins during Fcgamma-receptor-mediated phagocytosis in alveolar macrophages.

    abstract::Recent studies have demonstrated a central role for the exchange protein activated by cAMP (Epac) in the inhibition of Fcgamma-receptor-mediated phagocytosis and bacterial killing by prostaglandin E(2) (PGE(2)) in macrophages. However, the subcellular localization of Epac, and its primary target Rap1, has yet to be de...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brock TG,Serezani CH,Carstens JK,Peters-Golden M,Aronoff DM

    更新日期:2008-01-15 00:00:00

  • Induction of neurite outgrowth in PC12 cells by gamma-lactam-related compounds via Ras-MAP kinase signaling pathway independent mechanism.

    abstract::Rat pheochromocytoma cells, PC12 cells, undergo differentiation in response to nerve growth factor (NGF). Although the Ras-MAP kinase signaling pathway has been shown to play a central role in the response to NGF, the precise mechanism which induces differentiation remains unclarified. Recently, several gamma-lactam-r...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yao R,Osada H

    更新日期:1997-08-01 00:00:00

  • Chronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytes.

    abstract::TLR expression in neutrophils and monocytes is associated with increased cytokine synthesis, resulting in increased inflammation. However, the inflammatory pathway related to TLR and cathelicidin expression in these cells from CKD patients is unclear. To evaluate TLR4, cathelicidin, TNF-α, IL-6, IL-10 and MCP-1 expres...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grabulosa CC,Manfredi SR,Canziani ME,Quinto BMR,Barbosa RB,Rebello JF,Batista MC,Cendoroglo M,Dalboni MA

    更新日期:2018-04-15 00:00:00

  • Keratocytes are induced to produce collagen type II: A new strategy for in vivo corneal matrix regeneration.

    abstract::The stroma, the middle layer of the cornea, is a connective tissue making up most of the corneal thickness. The stromal extracellular matrix (ECM) consists of highly organised lamellae which are made up of tightly packed fibrils primarily composed of collagens type I and V. This layer is interspersed with keratocytes,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Greene CA,Green CR,Dickinson ME,Johnson V,Sherwin T

    更新日期:2016-09-10 00:00:00

  • SIRT1 and microRNAs: The role in breast, lung and prostate cancers.

    abstract::Breast cancer and prostate cancer are the most common malignant tumors in female and men, respectively. Furthermore, lung cancer is the leading cause of cancer deaths worldwide. It is an emergency to develop a powerful strategy to treat these threatening cancers more effectively, because of low efficacy and high rates...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Karbasforooshan H,Roohbakhsh A,Karimi G

    更新日期:2018-06-01 00:00:00

  • Developmental kinetics of hemopoietic progenitors in the avian embryo spleen.

    abstract::By means of plasma clot clonal cultures, the content of the avian spleen in granulomonocytic progenitors was studied during ontogeny. Serum-free media were used that were supplemented with growth activities produced either by embryonic fibroblasts or adult spleen cells. These two conditioned media not only permitted t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nicolas-Bolnet C,Yassine F,Cormier F,Dieterlen-Lièvre F

    更新日期:1991-10-01 00:00:00

  • Analysis of the inductive effect of the genomic equivalent of HALF1 sequence in the reversion of rat dedifferentiated hepatoma cells.

    abstract::HALF1 (human activator of liver function 1) is a closed DNA duplex implicated in reversion of rat dedifferentiated C2 hepatoma cells to a well-differentiated state. A copy of HALF1 is found in high-molecular-weight DNA in the human genome. The genomic equivalent of HALF1 and its flanking sequences [gH(5'-3') fragment]...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Boccaccio C,Meunier-Rotival M,Deschatrette J

    更新日期:1994-07-01 00:00:00

  • Disruption of cytoplasmic microtubules by ultraviolet radiation.

    abstract::Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zamansky GB,Perrino BA,Chou IN

    更新日期:1991-07-01 00:00:00

  • Betulinic acid derivatives can protect human Müller cells from glutamate-induced oxidative stress.

    abstract::Müller cells are the predominant retinal glial cells. One of the key roles of Müller cells is in the uptake of the neurotransmitter glutamate and in its conversion to glutamine. Müller cell dysfunction due to oxidative stress elicited by high glutamate concentrations can lead to toxicity, which promote the pathogenesi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cheng Z,Zhang T,Zheng J,Ding W,Wang Y,Li Y,Zhu L,Murray M,Zhou F

    更新日期:2019-10-01 00:00:00

  • Differential regulation of protooncogenes c-jun and jun D expressions by protein tyrosine kinase, protein kinase C, and cyclic-AMP mitogenic pathways in dog primary thyrocytes: TSH and cyclic-AMP induce proliferation but downregulate C-jun expression.

    abstract::The expressions of the protooncogenes c-jun and jun D have been investigated in dog thyrocytes in a primary culture whose proliferation is stimulated by three distinct intracellular signaling pathways (1) the thyrotropin (TSH) or forskolin-cyclic-AMP-mediated cascade; (2) the protein kinase C pathway activated by diac...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Reuse S,Pirson I,Dumont JE

    更新日期:1991-10-01 00:00:00

  • p53 checkpoint-defective cells are sensitive to X rays, but not hypoxia.

    abstract::X-ray-induced damage leads to cell-cycle "checkpoint" arrest by p53-dependent induction of the cyclin-dependent kinase inhibitor p21 (Waf1/Cip1/Sdi1). Human tumor cells that lack this response fail to arrest after exposure to DNA-damaging agents, undergo multiple rounds of endoreduplicative DNA synthesis, and eventual...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Denko NC,Green SL,Edwards D,Giaccia AJ

    更新日期:2000-07-10 00:00:00

  • Trafficking of receptor tyrosine kinases to the nucleus.

    abstract::It has been known for at least 20 years that growth factors induce the internalization of cognate receptor tyrosine kinases (RTKs). The internalized receptors are then sorted to lysosomes or recycled to the cell surface. More recently, data have been published to indicate other intracellular destinations for the inter...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Carpenter G,Liao HJ

    更新日期:2009-05-15 00:00:00

  • Analysis of mtDNA copy number and composition of single mitochondrial particles using flow cytometry and PCR.

    abstract::Mitochondrial DNA (mtDNA) is a multicopy, maternally inherited, genome. Individuals frequently carry a mixture of genetically distinct mtDNA molecules whose proportions may vary between sexual generations or among tissues from the same individual. Analyses of the genetic composition of mitochondria have previously rel...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cavelier L,Johannisson A,Gyllensten U

    更新日期:2000-08-25 00:00:00

  • The regulation of transcriptional repression in hypoxia.

    abstract::A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Cavadas MAS,Cheong A,Taylor CT

    更新日期:2017-07-15 00:00:00

  • Microinjected anti-actin antibodies decrease gap junctional intercellular commmunication in cultured astrocytes.

    abstract::The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Theiss C,Meller K

    更新日期:2002-12-10 00:00:00

  • Inhibition of miRNA-21 promotes retinal ganglion cell survival and visual function by modulating Müller cell gliosis after optic nerve crush.

    abstract:BACKGROUND:Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environme...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li HJ,Sun ZL,Pan YB,Sun YY,Xu MH,Feng DF

    更新日期:2019-02-15 00:00:00

  • Epigenetic activation of the 5-hydroxytryptamine (serotonin) receptor 2C in embryonal carcinoma cells is DNA replication-dependent.

    abstract::The epigenetic states of key regulatory genes must be altered to drive cell fate decisions in differentiating cells. This process must be coupled, at least transiently, to the DNA replication machinery. Only a few genes, however, have been shown to require DNA replication for their activation or repression upon induct...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bancescu DL,Glatt-Deeley H,Lalande M

    更新日期:2004-08-01 00:00:00

  • Coagulation factor Xa drives tumor cells into apoptosis through BH3-only protein Bim up-regulation.

    abstract::Coagulation Factor (F)Xa is a serine protease that plays a crucial role during blood coagulation by converting prothrombin into active thrombin. Recently, however, it emerged that besides this role in coagulation, FXa induces intracellular signaling leading to different cellular effects. Here, we show that coagulation...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Borensztajn KS,Bijlsma MF,Groot AP,Brüggemann LW,Versteeg HH,Reitsma PH,Peppelenbosch MP,Spek CA

    更新日期:2007-07-15 00:00:00

  • Nuclear gems and Cajal (coiled) bodies in fetal tissues: nucleolar distribution of the spinal muscular atrophy protein, SMN.

    abstract::SMN, the affected protein in spinal muscular atrophy (SMA), is a cytoplasmic protein that also occurs in nuclear structures called "gems" and is involved in snRNP maturation. Coilin-p80 is a marker protein for nuclear Cajal bodies (coiled bodies; CBs) which are also involved in snRNP maturation, storage or transport. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Young PJ,Le TT,Dunckley M,Nguyen TM,Burghes AH,Morris GE

    更新日期:2001-05-01 00:00:00

  • Isolation of a G0-specific ts mutant from a Fischer rat cell line, 3Y1.

    abstract::A ts mutant clone, tsJT60, was isolated from Fisher rat cell line, 3Y1. During the exponential growth at both 34 and 39.5 degrees C, tsJT60 did not appear as ts mutant cells. However, once entered resting state (G0) under serum deprivation at the confluent state, they could re-enter S phase at 34 degrees C but could n...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ide T,Ninomiya J,Ishibashi S

    更新日期:1984-01-01 00:00:00

  • Acute myocardial infarction leads to upregulation of the IGF-1 autocrine system, DNA replication, and nuclear mitotic division in the remaining viable cardiac myocytes.

    abstract::Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

    更新日期:1994-08-01 00:00:00

  • Localization of nucleoside triphosphatase activity to the inner nuclear envelope and associated heterochromatin.

    abstract::Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Clawson GA,Friend DS,Smuckler EA

    更新日期:1984-11-01 00:00:00

  • Changes in elastin-binding proteins during the phenotypic transition of rabbit arterial smooth muscle cells in primary culture.

    abstract::Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yamamoto K,Aoyagi M,Yamamoto M

    更新日期:1995-05-01 00:00:00

  • Cell-surface location and molecular properties of cell-CAM 105 in intestinal epithelial cells.

    abstract::Cell-CAM 105 is involved in intercellular adhesion of isolated rat hepatocytes in vitro. In addition to liver, cell-CAM 105 occurs in several different epithelia, in platelets, and in granulocytes. In this paper we present a detailed analysis of cell-CAM 105 in the small intestinal mucosa. Isolated rat intestinal epit...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hansson M,Blikstad I,Obrink B

    更新日期:1989-03-01 00:00:00

  • Isoform-specific attachment of osteoprogenitors to laminins: mapping to the short arms of laminin-1.

    abstract::The recruitment of osteoblast progenitors involves their migration and attachment to the sites of bone formation through interactions with matrix proteins. In a time-limited cell attachment assay, coated laminin-1 inhibits the adhesion of most rat calvaria cells but attaches specifically to osteoprogenitors, as quanti...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Roche P,Rousselle P,Lissitzky JC,Delmas PD,Malaval L

    更新日期:1999-08-01 00:00:00

  • The effect of a 10-day space flight on the function, phenotype, and adhesion molecule expression of splenocytes and lymph node lymphocytes.

    abstract::Previous studies have indicated that space flight affects the activation of lymphocytes from humans, monkeys, and rodents. In rats, where lymphocytes from blood, spleen, and lymph nodes have been tested, the accumulated data suggest that the effects of flight on various cells are lymphoid organ-specific. Thus, cells m...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grove DS,Pishak SA,Mastro AM

    更新日期:1995-07-01 00:00:00

  • Estrogen receptor alpha (ESR1)-signaling regulates the expression of the taxane-response biomarker PRP4K.

    abstract::The pre-mRNA splicing factor 4 kinase PRP4K (PRPF4B), is an essential kinase that is a component of the U5 snRNP and functions in spliceosome assembly. We demonstrated that PRP4K is a novel biological marker for taxane response in ovarian cancer patients and reduced levels of PRP4K correlate with intrinsic and acquire...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lahsaee S,Corkery DP,Anthes LE,Holly A,Dellaire G

    更新日期:2016-01-01 00:00:00