Abstract:
:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked to the 5'-end of the sequence for the green fluorescent protein. Upon induction, both types of transfectants overproduced nucleotidase activity in a time- and dose-dependent manner. Western blots gave values close to the expected subunit molecular masses of 65 and 92 kDa, respectively, excluding processing of the induced proteins. Cells induced to overexpress the nucleotidase showed a decreased growth rate and contained smaller pools of each of the four common ribonucleoside triphosphates. They showed no increased resistance to the toxicity of 2-chlorodeoxyadenosine.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi Vdoi
10.1006/excr.1999.4681keywords:
subject
Has Abstractpub_date
1999-12-15 00:00:00pages
474-82issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(99)94681-5journal_volume
253pub_type
杂志文章abstract::The giant protein hFat1, a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. To examine functions of the transmembrane and cytoplasmic domains, they were e...
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