Abstract：
:Autophagy is a cellular pathway involved in protein and organelle degradation. It is relevant to many types of cellular homeostasis and human diseases. High level of glucose is known to inflict podocyte injury, but little is reported about the relationship between high concentrations of glucose and autophagy in these cells. The present study demonstrates that high glucose promotes autophagy in podocytes. Rapamycin further enhances this effect, but 3-methyadenine inhibits it. The proautophagic effect of high glucose manifested in the form of enhanced podocyte expression of LC3-2 and beclin-1; interestingly, antioxidants such as NAC were found to inhibit high glucose-induced autophagy. High glucose induced the generation of ROS by podocytes in a time-dependent manner. High glucose also enhanced podocyte expression of MnSOD and catalase. These findings indicate that high glucose-induced autophagy is mediated through podocyte ROS generation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ma T,Zhu J,Chen X,Zha D,Singhal PC,Ding Gdoi
10.1016/j.yexcr.2013.01.018subject
Has Abstractpub_date
2013-04-01 00:00:00pages
779-89issue
6eissn
0014-4827issn
1090-2422pii
S0014-4827(13)00036-0journal_volume
319pub_type
杂志文章abstract：:The biological mechanisms involved in initiating, coordinating, and ultimately terminating cell-cell adhesion in the stratified epithelium are not well understood at present. This study was designed to elucidate the roles of the muscarinic M3, the nicotinic alpha3, and the mixed muscarinic-nicotinic alpha9 acetylcholi...
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