Mitochondrial membrane potential and DNA stainability in human sperm cells: a flow cytometry analysis with implications for male infertility.

abstract：:Total epidermal keratinocytes are a heterogeneous population of cells, including undifferentiated stem/progenitor cells (EpSPs) and their more differentiated progeny (Non-SP cells). Our previous in vivo data showed that EpSPs enhanced blood flow restoration when injected into an ischemic limb, whereas Non-SP cells had...

journal_title：Experimental cell research

authors： Oberley CC,Gourronc F,Hakimi S,Riordan M,Bronner S,Jiao C,Dunnwald M

更新日期：2008-02-15 00:00:00

abstract：:Heat shock factor 1 (Hsf1) is an ancient transcription factor that monitors protein homeostasis (proteostasis) and counteracts disturbances by triggering a transcriptional programme known as the heat shock response (HSR). The HSR is transiently activated and upregulates the expression of core proteostasis genes, inclu...

journal_title：Experimental cell research

authors： Masser AE,Ciccarelli M,Andréasson C

更新日期：2020-11-01 00:00:00

abstract：:Previous studies have shown that ascorbic acid increases both the total surface acetylcholine receptor (AChR) expression and the mRNA for the alpha-subunit of this receptor in myotubes of cloned L5 muscle cultures. Since ascorbic acid increases collagen synthesis in fibroblasts, we studied the effect of ascorbic acid ...

journal_title：Experimental cell research

authors： Liu E,Minor RR,Horovitz O,Wootton JA,Podleski TR,Salpeter MM

更新日期：1993-11-01 00:00:00

abstract：:Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in China with a poor prognosis. Most ESCC patients were diagnosed at advanced stages, losing the opportunity for surgical excision. Hence, it remains a pressing work to identify biomarkers for early detection, prognosis prediction and target...

journal_title：Experimental cell research

authors： Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

更新日期：2018-06-01 00:00:00

abstract：:Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of ...

journal_title：Experimental cell research

authors： Hirako Y,Yonemoto Y,Yamauchi T,Nishizawa Y,Kawamoto Y,Owaribe K

更新日期：2014-06-10 00:00:00

abstract：:1,25-dihydroxyvitamin D(3) (VD(3)) induces differentiation in a number of leukemia cell lines and under various conditions is able to either stimulate or inhibit nuclear factor kappa B (NF-kappaB) activity. Here we report a time-dependent biphasic regulation of NF-kappaB in VD(3)-treated HL-60 leukemia cells. After VD...

journal_title：Experimental cell research

authors： Tse AK,Wan CK,Shen XL,Zhu GY,Cheung HY,Yang M,Fong WF

更新日期：2007-05-01 00:00:00

abstract：:The binding of actinomycin D (actD) to fixed human metaphase chromosomes was studied by using autoradiography with [3H]actD and indirect immunofluorescence with a specific anti-actD antibody. At concentrations of 0.01 and 0.1 micrograms/ml there was a uniform distribution of drug along the chromosomes as observed by b...

journal_title：Experimental cell research

authors： Brothman AR,Lindell TJ

更新日期：1984-03-01 00:00:00

abstract：:PHOX2B and its paralogue gene PHOX2A are two homeodomain proteins in the network regulating the development of autonomic ganglia that have been associated with the pathogenesis of neuroblastoma (NB), because of their over-expression in different NB cell lines and tumour samples. We used the SK-N-BE(2)C cell line to sh...

journal_title：Experimental cell research

authors： Di Lascio S,Saba E,Belperio D,Raimondi A,Lucchetti H,Fornasari D,Benfante R

更新日期：2016-03-01 00:00:00

abstract：:We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain...

journal_title：Experimental cell research

authors： Ishikawa M,Kuroda Y,Kobayashi K,Sawada H,Hayashi M

更新日期：1991-03-01 00:00:00

abstract：:It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...

journal_title：Experimental cell research

authors： Tabellini G,Bortul R,Santi S,Riccio M,Baldini G,Cappellini A,Billi AM,Berezney R,Ruggeri A,Cocco L,Martelli AM

更新日期：2003-07-01 00:00:00

abstract：OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...

journal_title：Experimental cell research

authors： Zhang GW,Wen T,Gu TX,Li-Ling J,Wang C,Zhao Y,Liu J,Wang Y,Liu TJ,Lü F

更新日期：2012-02-15 00:00:00

abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...

journal_title：Experimental cell research

authors： Tchang F,Méchali M

更新日期：1999-08-25 00:00:00

abstract：:The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular basement membrane (GBM), a specialized extracellular matrix that lies between them. Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteog...

journal_title：Experimental cell research

authors： Miner JH

更新日期：2012-05-15 00:00:00

abstract：:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velo...

journal_title：Experimental cell research

authors： Ye XH,Metcalf TN 3rd,Andrews DM,Carraway CA,Carraway KL

更新日期：1989-05-01 00:00:00

abstract：:We have studied the mechanisms of angiotensin II (A-II) transport through a cultured arterial endothelial cell monolayer. The transport of 125I-labeled A-II was inhibited by excess unlabeled A-II (50 microM) and [Sar1, Ile8]-A-II (50 microM), but was not inhibited by bradykinin (50 microM). The transport process was s...

journal_title：Experimental cell research

authors： Mineo C,Yagyu Y,Imanaka T,Takano T

更新日期：1990-09-01 00:00:00

abstract：:SMN, the affected protein in spinal muscular atrophy (SMA), is a cytoplasmic protein that also occurs in nuclear structures called "gems" and is involved in snRNP maturation. Coilin-p80 is a marker protein for nuclear Cajal bodies (coiled bodies; CBs) which are also involved in snRNP maturation, storage or transport. ...

journal_title：Experimental cell research

authors： Young PJ,Le TT,Dunckley M,Nguyen TM,Burghes AH,Morris GE

更新日期：2001-05-01 00:00:00

abstract：:The growth rate of malignant F9 embryonal carcinoma cells slows considerably following all-trans-retinoic acid-induced differentiation into benign parietal endoderm. To determine the mechanism of this process, we examined the expression of cyclins D1, D2, and D3 and the activity of their associated kinases. Cyclin D1 ...

journal_title：Experimental cell research

authors： Li Y,Glozak MA,Smith SM,Rogers MB

更新日期：1999-12-15 00:00:00

abstract：:The human neuroblastoma cell line SK-N-BE can be induced to differentiate by retinoic acid (RA) or by alpha-difluoromethylornithine (DFMO). The former inducer produces neurite outgrowth, 60% reduction of growth rate, overexpression of neural antigens, and enhanced gamma-aminobutyric acid (GABA) and acetylcholinesteras...

journal_title：Experimental cell research

authors： Melino G,Farrace MG,Ceru' MP,Piacentini M

更新日期：1988-12-01 00:00:00

abstract：:Hypoxia can activate the endothelium toward a pro-inflammatory phenotype and enhance leukocyte adhesion. This process is involved in pathological conditions such as vascular remodeling or ischemia-reperfusion injury. This study was aimed to obtain a global picture of the response of the endothelial cells to hypoxia wi...

journal_title：Experimental cell research

authors： Flamant L,Toffoli S,Raes M,Michiels C

更新日期：2009-03-10 00:00:00

abstract：:Skin extracellular matrix (ECM) molecules regulate a variety of cellular activities, including cell movement, which are central to wound healing and metastasis. Regulated cell movement is modulated by proteases and their associated molecules, including the serine proteases urinary-type plasminogen activator (uPA) and ...

journal_title：Experimental cell research

authors： Jones JM,Cohen RL,Chambers DA

更新日期：2002-11-01 00:00:00

abstract：:The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...

journal_title：Experimental cell research

authors： Takakubo F,Dahl HH

更新日期：1992-03-01 00:00:00

abstract：:Regulatory B cells (Breg cells) play critical roles in modulating immune responses during autoimmune diseases and infection. Here we explored the participation of two main Breg subsets, including IL-10+ Breg (B10) and IL-35+ Breg cells, in maintaining successful pregnancy. We first observed an elevated percentage of B...

journal_title：Experimental cell research

authors： Liu J,Chen X,Hao S,Zhao H,Pang L,Wang L,Ren H,Wang C,Mao H

更新日期：2019-10-15 00:00:00

abstract：:Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the eff...

journal_title：Experimental cell research

authors： Xie D,Liu Z,Wu J,Feng W,Yang K,Deng J,Tian G,Santos S,Cui X,Lin F

更新日期：2017-08-01 00:00:00

abstract：:p107 and p130 were originally identified as targets of the transforming domains of viral oncoproteins encoded by small DNA tumor viruses. Together with pRB, the protein product of the retinoblastoma gene (Rb), p107 and p130 represent a family of closely related proteins that play critical roles in the regulation of ce...

journal_title：Experimental cell research

authors： Classon M,Dyson N

更新日期：2001-03-10 00:00:00

abstract：:Various cell adhesion molecules mediate the diverse functions of the vascular endothelium, such as cell adhesion, neutrophil migration, and angiogenesis. In order to identify cell adhesion molecules important for angiogenesis, we used an in vitro model (Chalupowicz, Chowdhury, Bach, Barsigian, and Martinez, J. Cell Bi...

journal_title：Experimental cell research

authors： Bach TL,Barsigian C,Chalupowicz DG,Busler D,Yaen CH,Grant DS,Martinez J

更新日期：1998-02-01 00:00:00

abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

journal_title：Experimental cell research

authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

更新日期：1998-05-25 00:00:00

abstract：:ADvF11 (F11), a Chinese Hamster Ovary (CHO) cell variant, is defective in its ability to adhere to fibronectin (Fn)-coated substrata but will adhere to substrata coated with poly-L-lysine, conA or extracellular matrix (ECM) [1]. We have observed that both F11 and CHO wild-type (WT) cells were able to bind 3H-Fn beads ...

journal_title：Experimental cell research

authors： Cheung E,Juliano RL

更新日期：1984-05-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) represents 0.15% of the total cell DNA (at least an order of magnitude less than in liver or heart) of rabbit articular chondrocytes. Besides the already well-documented low respiratory activity, chondrocyte differentiation thus involves a specific control of mitochondrial biogenesis. When tr...

journal_title：Experimental cell research

authors： Champagne AM,Benel L,Ronot X,Mignotte F,Adolphe M,Mounolou JC

更新日期：1987-08-01 00:00:00

abstract：:Annulate lamellae (AL) are cytoplasmic arrays of stacked membrane cisternae containing densely packed pore complexes which are similar in structure to the nuclear pore complexes (NPCs) and thus referred to as annulate lamella pore complexes (ALPCs). We have recently shown that the integral nuclear pore membrane protei...

journal_title：Experimental cell research

authors： Imreh G,Hallberg E

更新日期：2000-08-25 00:00:00

abstract：:Pirh2, a recently identified ubiquitin-protein ligase, has been reported to promote p53 degradation. Pirh2 physically interacts with p53 and promotes ubiquitination of p53 independently of MDM2. Like MDM2, Pirh2 is thought to participate in an autoregulatory feedback loop that controls p53 function. We have previously...

journal_title：Experimental cell research

authors： Duan W,Gao L,Wu X,Zhang Y,Otterson GA,Villalona-Calero MA

更新日期：2006-10-15 00:00:00