Abstract：
:Total epidermal keratinocytes are a heterogeneous population of cells, including undifferentiated stem/progenitor cells (EpSPs) and their more differentiated progeny (Non-SP cells). Our previous in vivo data showed that EpSPs enhanced blood flow restoration when injected into an ischemic limb, whereas Non-SP cells had no significant effect on in vivo blood flow restoration. However, the cellular and molecular mechanisms of this observation remain largely unknown. Therefore, the aim of this study was to investigate the angiogenic properties of different epidermal subpopulations in vitro and the mechanism by which EpSPs enhanced blood flow in vivo. Using migration assay and capillary network formation, we show that EpSPs secrete higher levels of pro-angiogenic molecules compared to Non-SP cells, unsorted keratinocytes and fibroblasts in vitro. Secretion of vascular endothelial growth factor (VEGF) was detected at higher levels in EpSP conditioned medium than the medium conditioned by other epidermal subpopulations and fibroblasts. Also, RT-PCR analyses revealed a unique angiogenic gene profile for EpSPs. Finally, gene array data indicate significant changes in angiogenic gene expression six days after cell injection in murine ischemic limbs. Therefore, we conclude that EpSPs possess unique angiogenic properties and that these cells may be indirectly responsible for the angiogenic response previously observed in our ischemic limb model.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Oberley CC,Gourronc F,Hakimi S,Riordan M,Bronner S,Jiao C,Dunnwald Mdoi
10.1016/j.yexcr.2007.11.002subject
Has Abstractpub_date
2008-02-15 00:00:00pages
720-8issue
4eissn
0014-4827issn
1090-2422pii
S0014-4827(07)00529-0journal_volume
314pub_type
杂志文章abstract：:Increased protein aggregation and altered cell signaling accompany many neurodegenerative diseases including Huntington's disease (HD). Cell stress is counterbalanced by signals mediating cell repair but the identity of these are not fully understood. We show here that the mammalian target of rapamycin (mTOR) pathway ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.08.020
更新日期：2012-01-01 00:00:00
abstract：:In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90330-8
更新日期：1988-06-01 00:00:00
abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.04.043
更新日期：2004-08-15 00:00:00
abstract：:The biological mechanisms involved in initiating, coordinating, and ultimately terminating cell-cell adhesion in the stratified epithelium are not well understood at present. This study was designed to elucidate the roles of the muscarinic M3, the nicotinic alpha3, and the mixed muscarinic-nicotinic alpha9 acetylcholi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.12.010
更新日期：2004-04-01 00:00:00
abstract：:Like most epithelial cells that are isolated from tissues and placed in culture, the phenotype of human retinal pigment epithelial cells (RPE) in vitro is more variable than for the same cells in situ. The phenotypic heterogeneity of the cultures has been attributed to varying stages of differentiation of the cells in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1176
更新日期：1994-07-01 00:00:00
abstract：:The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111958
更新日期：2020-05-15 00:00:00
abstract：:Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90401-f
更新日期：1991-08-01 00:00:00
abstract：:Src-family kinases, cytoplasmic enzymes that participate in various signaling events, are found at not only the plasma membrane but also subcellular compartments, such as the nucleus, the Golgi apparatus and late endosomes/lysosomes. Lyn, a member of the Src-family kinases, is known to play a role in DNA damage respon...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.08.019
更新日期：2008-11-01 00:00:00
abstract：:Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90061-x
更新日期：1989-02-01 00:00:00
abstract：:Drosophila sHSPs (small heat shock proteins) are expressed in the absence of stress in specific regions of the central nervous system and in gonads of young adults flies. In these two organs, the sHSPs show a cell-specific and developmental stage-specific pattern of expression suggesting distinct regulation and functi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0052
更新日期：1996-02-25 00:00:00
abstract：:Seven continuous cell lines have been established in vitro from lethal embryos produced by the female sterile mutant mh 1182 of Drosophila melanogaster. Six lines show haploid metaphases. Karyotype analysis revealed a high level of aneuploid cells with frequent chromosome fragments. In three lines, haploid cells were ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90371-9
更新日期：1984-03-01 00:00:00
abstract：:Promyelocytic leukemia nuclear bodies (PML-NBs) are implicated in transcriptional regulation. Here we identify a novel transcriptional repressor, PML-associated repressor of transcription (PAROT), which is regulated in its repressor activity through recruitment to PML-NBs. PAROT is a Krüppel-associated box ( KRAB) zin...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.005
更新日期：2006-04-01 00:00:00
abstract：:It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112169
更新日期：2020-10-01 00:00:00
abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.03.018
更新日期：2010-07-15 00:00:00
abstract：:Cell surface beta-1,4-galactosyltransferase (GalTase) partially mediates a variety of cell interactions with laminin-containing matrices, including mesenchymal cell spreading and migration and neurite initiation, by binding to N-linked oligosaccharides within the E8 domain of laminin. Previous studies using indirect i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90350-h
更新日期：1992-07-01 00:00:00
abstract：:Interleukin-1 (IL-1) is one of the most potent bone-resorbing factors involved in bone loss associated with inflammation. We previously reported that IL-1 prolonged the survival of multinucleated osteoclast-like cells (OCLs) formed in cocultures of murine osteoblasts/stromal cells and bone marrow cells via the prevent...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4320
更新日期：1999-02-25 00:00:00
abstract：:The fruit-fly Drosophila melanogaster harbours different types of ciliary structures: ciliary projections associated with neurons of type I and cilium-like regions (CLRs) found during male gametogenesis. The latter deserve particular attention since they are morphologically similar to vertebrate primary cilia and tran...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.08.020
更新日期：2018-10-01 00:00:00
abstract：:Leptomycin B (LMB) is a Streptomyces metabolite that inhibits nuclear export of the human immunodeficiency virus type 1 regulatory protein Rev at low nanomolar concentrations. Recently, LMB was shown to inhibit the function of CRM1, a receptor for the nuclear export signal (NES). Here we show evidence that LMB binds d...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4136
更新日期：1998-08-01 00:00:00
abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.01.017
更新日期：2016-06-10 00:00:00
abstract：:In the fungus Achlya, sexual development in the male strain E87 is mediated by the steroid hormone antheridiol. Treatment of vegetatively growing cells of E87 with antheridiol resulted in changes in the [35S]methionine labeling of several secreted proteins. The most heavily labeled group of proteins observed in the se...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90555-0
更新日期：1986-03-01 00:00:00
abstract：:We have investigated the role played by the growth and differentiation factor (GDF)-induced calcium ion second messenger signal in the control of cellular differentiation and proliferation in Syrian hamster embryo (SHE) cells. Blocking the platelet-derived growth factor (PDGF)-induced calcium ion signal with either ex...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0237
更新日期：1996-08-01 00:00:00
abstract：:Colon cancer shows characteristics of metastasis, which is associated with angiogenesis. Increasing evidence highlights long non-coding RNAs (lncRNAs) as important participants in angiogenesis of cancers, including colon cancer. Hence, this study investigated the role of HNF1A-AS1 in angiogenesis of colon cancer. RT-q...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112025
更新日期：2020-08-15 00:00:00
abstract：:The intracellular distribution of the glucocorticosteroid and progesterone receptors (GR and PR, respectively) was studied immunohistochemically. In control adrenalectomized (Adx) rat liver, immunostaining of paraffin sections revealed GR in cell nuclei, with a wide range of intensity between individuals. Following de...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90106-7
更新日期：1989-04-01 00:00:00
abstract：:We generated four monoclonal antibodies (mAbs) specific for human alpha 3 integrin (VLA-3 alpha subunit). All of them were found to induce homotypic cell aggregation of HT1080 fibrosarcoma and SN12C renal carcinoma cells, both of which express high levels of alpha 3 integrin. The antibodies also induced the cell aggre...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1069
更新日期：1994-03-01 00:00:00
abstract：:Abscission marks the completion of cell division and its failure is associated with delayed cytokinesis and even tetraploidization. Aberrant abscission and consequential ploidy changes can underlie various diseases including cancer. Midbody, a transient structure formed in the intercellular bridge during telophase, co...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.08.019
更新日期：2011-01-15 00:00:00
abstract：:Patients infected with human immunodeficiency virus type 1 (HIV-1) develop a spectrum of B cell lymphoproliferative disorders ranging from polyclonal B cell activation to B cell lymphomas. While a direct role of Epstein-Barr virus (EBV) is well recognized for most of these lesions, recent findings have suggested that ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.01.018
更新日期：2004-05-01 00:00:00
abstract：:Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR1)....
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.021
更新日期：2005-03-10 00:00:00
abstract：:Calcium-tolerant myocytes were isolated from adult rat hearts by collagenase perfusion and plated on various substrates in serum-free medium and their adhesion to various extracellular matrix (ECM) components was determined. The myocytes attached readily to dishes coated with collagen type IV (C-IV), laminin (LN), and...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90462-8
更新日期：1985-06-01 00:00:00
abstract：:Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell prolife...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.01.025
更新日期：2011-04-15 00:00:00
abstract：:In this study an amphotropic retrovirus has been used to efficiently transduce normal human (NF) and scleroderma (systemic sclerosis; SSc) dermal fibroblasts (SScF) with a sequence encoding a temperature-sensitive mutant of the SV40 large T antigen (tsA58-U19). From the primary outgrowths of skin explants, cultures we...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1332
更新日期：1995-10-01 00:00:00
