Abstract：
:Calcium-tolerant myocytes were isolated from adult rat hearts by collagenase perfusion and plated on various substrates in serum-free medium and their adhesion to various extracellular matrix (ECM) components was determined. The myocytes attached readily to dishes coated with collagen type IV (C-IV), laminin (LN), and to fetal bovine serum (FBS) in a manner dependent on the concentration of the components. Substantially fewer myocytes adhered to dishes coated with fibronectin (FN) or to uncoated plastic dishes. Cells adhered equally well to dishes coated with C-IV, LN and FBS within 1-4 h. However, when examined after 2 weeks in culture it was found that only C-IV and LN could support survival of the attached myocytes, and when cultured on C-IV or LN the myocytes were spread and had formed a dense monolayer. The actin filaments had at this time reorganized linearly along the long axis of the cell and the myocytes contracted spontaneously. Rabbit antibodies were raised against myocyte membranes and their ability to inhibit attachment to ECM components was studied. Purified IgG inhibited attachment to C-IV, while having only a minor effect on attachment to LN. These data are compatible with the presence of a specific cell surface component(s) that interacts with ECM substrates and influences cell shape and possibly thereby influences cellular functions.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Lundgren E,Terracio L,Mårdh S,Borg TKdoi
10.1016/0014-4827(85)90462-8subject
Has Abstractpub_date
1985-06-01 00:00:00pages
371-81issue
2eissn
0014-4827issn
1090-2422journal_volume
158pub_type
杂志文章abstract：:The pleiotropic cytokine interleukin (IL)-10 is best characterized by its ability to downregulate inflammation and promote peripheral tolerance. On the other hand, IL-10 was also found to maintain the effector response of CD8+ T cells and promote the expansion of tumor-resident CD8+ T cells. In diffuse large B cell ly...
journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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doi：10.1016/0014-4827(85)90240-x
更新日期：1985-09-01 00:00:00
abstract：:The effect of RU 486, a synthetic steroid that is a powerful antagonist of glucocorticoid hormones, was tested on the transcription of several glucocorticoid-regulated genes in different cell types: inflammatory murine macrophages and two human mammary gland-derived cell lines, MDA-MB-231 and HBL-100. The transcriptio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90282-5
更新日期：1987-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4121
更新日期：1998-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00032-6
更新日期：2003-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2001-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.06.018
更新日期：2012-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1986-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111746
更新日期：2020-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90162-4
更新日期：1985-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.10.004
更新日期：2016-11-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90288-7
更新日期：1989-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1114
更新日期：1995-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(05)80080-1
更新日期：1992-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.04.008
更新日期：2005-08-01 00:00:00
abstract：:The activation of Cdc2 kinase induces the entry into M-phase of all eukaryotic cells. We have developed a cell-free system prepared from prophase-arrested Xenopus oocytes to analyze the mechanism initiating the all-or-none activation of Cdc2 kinase. Inhibition of phosphatase 2A, the major okadaic acid-sensitive Ser/Th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4220
更新日期：1998-11-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2006.07.002
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journal_title：Experimental cell research
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doi：10.1006/excr.2002.5596
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2014-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.02.013
更新日期：2010-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90123-c
更新日期：1991-05-01 00:00:00
abstract：:The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.10.007
更新日期：2009-01-01 00:00:00
abstract：:Tendon is a critical unit of musculoskeletal system that connects muscle to bone to control bone movement. More population participate in physical activities, tendon injuries, such as acute tendon rupture and tendinopathy due to overuse, are common causing unbearable pain and disability. However, the process of tendon...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2015.12.014
更新日期：2016-02-15 00:00:00
abstract：:Tendinopathy is a common and disabling condition that is difficult to treat. The pathomolecular events behind tendinopathy remain uncertain. Micro-RNAs (miRNAs, miRs) are short non-coding RNAs that regulate gene expression and may play a role in tendinopathy development. Tenocytes were obtained from human patellar ten...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.03.008
更新日期：2019-05-15 00:00:00
abstract：:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.006
更新日期：2005-02-15 00:00:00
abstract：:Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0258
更新日期：1996-08-25 00:00:00
abstract：:A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0078
更新日期：1996-03-15 00:00:00
