Exogenous micro-RNA and antagomir modulate osteogenic gene expression in tenocytes.

abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

journal_title：Experimental cell research

authors： Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

更新日期：2006-01-01 00:00:00

abstract：:C-KIT encodes a tyrosine kinase receptor (KIT) that, when activated by its ligand (KL), stimulates proliferation, differentiation, migration, and survival. Greater than 70% of epithelial ovarian cancers coexpress c-KIT and KL. C-KIT and KL expression levels have been shown to be up-regulated by cAMP in some cell types...

journal_title：Experimental cell research

authors： Shaw TJ,Keszthelyi EJ,Tonary AM,Cada M,Vanderhyden BC

更新日期：2002-02-01 00:00:00

abstract：:Transforming growth factor beta regulates many biological processes including cell motility and invasion. Podosomes are specialized F-actin rich structures found in normal cells, such as osteoclasts and macrophages. Tumor cells often form related structures called invadopodia that are thought to promote invasion and m...

journal_title：Experimental cell research

authors： Mandal S,Johnson KR,Wheelock MJ

更新日期：2008-11-15 00:00:00

abstract：:The nucleolar precursor bodies (NPBs) are electrondense, homogeneous, and finely filamentous intranuclear structures occurring in mammalian zygotes during the early postfertilization development and subsequently transforming into nucleoli. In this study we addressed the question if the assembly of the NPBs is correlat...

journal_title：Experimental cell research

authors： Tesarik J,Kopecny V

更新日期：1990-11-01 00:00:00

abstract：:We performed liquid chromatography-tandem mass spectrometry (LC-MS/MS) on control and TGF-β1-exposed rat lung fibroblasts to identify proteins differentially expressed between cell populations. A total of 196 proteins were found to be differentially expressed in response to TGF-β1 treatment. Guided by these results, w...

journal_title：Experimental cell research

authors： Na M,Hong X,Fuyu J,Dingjie X,Sales D,Hui Z,Zhongqiu W,Shifeng L,Xuemin G,Wenchen C,Dan L,Guizhen Z,Bonan Z,Lijuan Z,Shumin L,Ying Z,Jin W,Mingwang R,Summer R,Fang Y

更新日期：2019-02-15 00:00:00

abstract：:The biosynthesis of type XI and type II collagens was examined using a stable rat chondrocyte cell line established by W. E. Horton et al. (1988, Exp. Cell Res. 178, 457-468.). These cells (IRC; immortalized rat chondrocytes) were created by transformation with a murine retrovirus carrying the v-myc and v-raf oncogene...

journal_title：Experimental cell research

authors： Oxford JT,Doege KJ,Horton WE Jr,Morris NP

更新日期：1994-07-01 00:00:00

abstract：:Ataxia-telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and ...

journal_title：Experimental cell research

authors： Cooper TJ,Wardell K,Garcia V,Neale MJ

更新日期：2014-11-15 00:00:00

abstract：:Keratinocytes in human skin undergo apoptosis during various inflammatory processes and after ultraviolet (UV) irradiation. To determine if keratinocyte apoptosis may be mediated by the Fas/APO-1 receptor (CD95), a signal transduction pathway known to initiate programmed cell death of lymphocytes, we investigated Fas ...

journal_title：Experimental cell research

authors： Leverkus M,Yaar M,Gilchrest BA

更新日期：1997-05-01 00:00:00

abstract：:Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive...

journal_title：Experimental cell research

authors： Numata O,Tomiyoshi T,Kurasawa Y,Fu ZX,Takahashi M,Kosaka T,Chiba J,Watanabe Y

更新日期：1991-03-01 00:00:00

abstract：:Subsets of glycoproteins are thought to require lectin-like membrane receptors for efficient export out of the endoplasmic reticulum (ER). To identify new members related to two previously characterized intracellular lectins ERGIC-53/p58 and VIP36, we carried out an extensive database search using the conserved carboh...

journal_title：Experimental cell research

authors： Neve EP,Svensson K,Fuxe J,Pettersson RF

更新日期：2003-08-01 00:00:00

abstract：:We generated four monoclonal antibodies (mAbs) specific for human alpha 3 integrin (VLA-3 alpha subunit). All of them were found to induce homotypic cell aggregation of HT1080 fibrosarcoma and SN12C renal carcinoma cells, both of which express high levels of alpha 3 integrin. The antibodies also induced the cell aggre...

journal_title：Experimental cell research

authors： Takeuchi K,Tsuji T,Hakomori S,Irimura T

更新日期：1994-03-01 00:00:00

abstract：:The effects of methylmercury (MeHg) on cytoplasmic microtubules in cultured fibroblasts and on the in vitro polymerization of microtubules were examined. MeHg caused disruption of cellular microtubules in a concentration- and time-dependent manner. Addition of the metal-chelating agent, dimercaptosuccinic acid (DMSA),...

journal_title：Experimental cell research

authors： Sager PR,Doherty RA,Olmsted JB

更新日期：1983-06-01 00:00:00

abstract：:The enzyme activity hydrolysing diadenosine 5,5'-P1, P4-tetraphosphate (AP4A) was demonstrated in the embryonic extract of sea urchin. The enzyme activity was preferentially inhibited by ZnCl2 and by high concentrations of isobutylmethylxanthine, indicating that two types of the enzyme, (AP4A) hydrolase and non-specif...

journal_title：Experimental cell research

authors： Morioka M,Shimada H

更新日期：1987-03-01 00:00:00

abstract：:SIRT6, with both deacetylase and ADP-ribosyltransferase activities, is predominantly expressed in the nucleus. It has been revealed that SIRT6 regulates various biological functions including metabolism, aging and stress resistance. This study aims to investigate the role of SIRT6 in vascular inflammation and it molec...

journal_title：Experimental cell research

authors： He Y,Xiao Y,Yang X,Li Y,Wang B,Yao F,Shang C,Jin Z,Wang W,Lin R

更新日期：2017-08-01 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：:The basic helix-loop-helix/leucine zipper (bHLH/ZIP) microphthalmia-associated transcription factor (MITF) regulates transcription of genes encoding enzymes essential for melanin biosynthesis in melanocytes and retinal pigmented epithelial cells. To determine how MITF activity is regulated, we used the yeast two-hybri...

journal_title：Experimental cell research

authors： Xu W,Gong L,Haddad MM,Bischof O,Campisi J,Yeh ET,Medrano EE

更新日期：2000-03-15 00:00:00

abstract：:The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...

journal_title：Experimental cell research

authors： Cui L,Li M,Feng F,Yang Y,Hang X,Cui J,Gao J

更新日期：2014-10-15 00:00:00

abstract：:For cancer metastasis, tumor cells present in the circulation must first adhere to the endothelium. Integrins play a central role in leukocyte adhesion to the endothelium and subsequent migration into tissues. The majority of tumor cells derived from solid cancers, including breast cancer, do not express integrins. We...

journal_title：Experimental cell research

authors： Mine S,Fujisaki T,Kawahara C,Tabata T,Iida T,Yasuda M,Yoneda T,Tanaka Y

更新日期：2003-08-01 00:00:00

abstract：:Transforming growth factor-beta (TGF-beta) induces apoptosis in fetal rat hepatocytes. However, a subpopulation of these cells survives, concomitant with changes in phenotype, reminiscent of an epithelial-mesenchymal transition (EMT). We have previously suggested that EMT might confer cell resistance to apoptosis (Val...

journal_title：Experimental cell research

authors： Del Castillo G,Murillo MM,Alvarez-Barrientos A,Bertran E,Fernández M,Sánchez A,Fabregat I

更新日期：2006-09-10 00:00:00

abstract：:Hypoxic blood-brain barrier (BBB) dysfunction is a common feature of CNS diseases however mechanisms underlying barrier disturbance are still largely unknown. This study investigated the role of transforming growth factor β (TGFβ), a cytokine known to induce expression of the proprotein convertase Furin, in hypoxia-me...

journal_title：Experimental cell research

authors： Baumann J,Huang SF,Gassmann M,Tsao CC,Ogunshola OO

更新日期：2019-10-15 00:00:00

abstract：:Overexpression of P-glycoprotein may cause increased efflux of a variety of anticancer drugs (ACD) leading to multidrug resistance (MDR) of tumor cells. Two sublines of murine monocytic leukemia P388 cells were used, one parental (Par-P388) and one multidrug resistant (MDR-P388). In cell growth inhibition assays in vi...

journal_title：Experimental cell research

authors： Boesch D,Muller K,Pourtier-Manzanedo A,Loor F

更新日期：1991-09-01 00:00:00

abstract：:The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...

journal_title：Experimental cell research

authors： Kitajima Y,Owaribe K,Nishizawa Y,Jokura Y,Yaoita H

更新日期：1992-11-01 00:00:00

abstract：:The chemokine macrophage inflammatory protein-1 alpha [MIP-1alpha] causes migration of B cells and also induces changes in antibody secretion. However, the signal transduction pathways leading to these phenotypic changes remain undefined. We have identified a signal transduction pathway initiated by MIP-1alpha in B ce...

journal_title：Experimental cell research

authors： Rumsey LM,Teague RM,Benedict SH,Chan MA

更新日期：2001-08-01 00:00:00

abstract：:We have used biotinylated rDNA probes to localize by in situ hybridization the extrachromosomal genes for ribosomal RNA in the slime mold Physarum polycephalum. We established conditions that allow for highly specific hybridization at the ultrastructural level and determined that the 60-kb palindromic rDNA molecules a...

journal_title：Experimental cell research

authors： Puvion-Dutilleul F,Pierron G

更新日期：1992-12-01 00:00:00

abstract：:Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

journal_title：Experimental cell research

authors： Halleck MS,Schlegel RA

更新日期：1983-09-01 00:00:00

abstract：:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...

journal_title：Experimental cell research

authors： Hamilton BT,Snyder JA

更新日期：1983-04-01 00:00:00

abstract：:It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...

journal_title：Experimental cell research

authors： Marini M,Frabetti F,Canaider S,Dini L,Falcieri E,Poirier GG

更新日期：2001-06-10 00:00:00

abstract：:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

journal_title：Experimental cell research

authors： Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

更新日期：1996-04-10 00:00:00

abstract：:Hypoxia can activate the endothelium toward a pro-inflammatory phenotype and enhance leukocyte adhesion. This process is involved in pathological conditions such as vascular remodeling or ischemia-reperfusion injury. This study was aimed to obtain a global picture of the response of the endothelial cells to hypoxia wi...

journal_title：Experimental cell research

authors： Flamant L,Toffoli S,Raes M,Michiels C

更新日期：2009-03-10 00:00:00

abstract：:The epigenetic states of key regulatory genes must be altered to drive cell fate decisions in differentiating cells. This process must be coupled, at least transiently, to the DNA replication machinery. Only a few genes, however, have been shown to require DNA replication for their activation or repression upon induct...

journal_title：Experimental cell research

authors： Bancescu DL,Glatt-Deeley H,Lalande M

更新日期：2004-08-01 00:00:00