Abstract:
:We performed liquid chromatography-tandem mass spectrometry (LC-MS/MS) on control and TGF-β1-exposed rat lung fibroblasts to identify proteins differentially expressed between cell populations. A total of 196 proteins were found to be differentially expressed in response to TGF-β1 treatment. Guided by these results, we next determined whether similar changes in protein expression were detectable in the rat lung after chronic exposure to silica dust. Of the five proteins selected for further analysis, we found that levels of all proteins were markedly increased in the silica-exposed rat lung, including the proteins for the very low density lipoprotein receptor (VLDLR) and the transmembrane (type I) heparin sulfate proteoglycan called syndecan 2 (SDC2). Because VLDLR and SDC2 have not, to our knowledge, been previously linked to the pathobiology of silicosis, we next examined whether knockdown of either gene altered responses to TGF-β1 in MRC-5 lung fibroblasts. Interestingly, we found knockdown of either VLDLR or SDC2 dramatically reduced collagen production to TGF-β1, suggesting that both proteins might play a novel role in myofibroblast biology and pathogenesis of silica-induced pulmonary fibrosis. In summary, our findings suggest that performing LC-MS/MS on TGF-β1 stimulated lung fibroblasts can uncover novel molecular targets of activated myofibroblasts in silica-exposed lung.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Na M,Hong X,Fuyu J,Dingjie X,Sales D,Hui Z,Zhongqiu W,Shifeng L,Xuemin G,Wenchen C,Dan L,Guizhen Z,Bonan Z,Lijuan Z,Shumin L,Ying Z,Jin W,Mingwang R,Summer R,Fang Ydoi
10.1016/j.yexcr.2019.01.010subject
Has Abstractpub_date
2019-02-15 00:00:00pages
1-9issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(18)31094-2journal_volume
375pub_type
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