Abstract:
:It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of micromolar amounts of a Zn(2+) chelator allowed DNA cleavage at internucleosomal sites. Such results were extended in the present work, thus allowing their framing into the events related to alterations in the redox state of the cell. Apoptosis in hydrogen peroxide-treated lymphocytes was found to occur with caspase-3 activation, but the enzyme activity was found to be impaired, thus affecting internucleosomal fragmentation as well as nuclear morphology. Caspase-3 activity was found to resume upon mild Zn(2+) chelation. These results provide as well an experimental model from which apoptotic events upstream and downstream of caspase-3 activity can be examined.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Marini M,Frabetti F,Canaider S,Dini L,Falcieri E,Poirier GGdoi
10.1006/excr.2001.5222keywords:
subject
Has Abstractpub_date
2001-06-10 00:00:00pages
323-32issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(01)95222-Xjournal_volume
266pub_type
杂志文章abstract::A method for isolation of C-cells from rat fetuses was developed, and the morphological plasticity of the cells in primary culture systems was tested. Thyroid-parathyroid-ultimobranchial body (UB) complexes from 16-day rat fetuses were treated with 0.1% collagenase and 1000 PU/ml Dispase at 37 degrees C for 1 h. After...
journal_title:Experimental cell research
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abstract::Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death...
journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:2004-01-15 00:00:00
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doi:10.1016/j.yexcr.2014.10.015
更新日期:2015-02-15 00:00:00
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journal_title:Experimental cell research
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更新日期:1989-02-01 00:00:00
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更新日期:2015-04-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.10.012
更新日期:2005-02-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1999-12-15 00:00:00
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更新日期:2007-07-15 00:00:00
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更新日期:1986-09-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2015-01-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1998-12-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4432
更新日期:1999-05-01 00:00:00
abstract::HALF1 (human activator of liver function 1) is a closed DNA duplex implicated in reversion of rat dedifferentiated C2 hepatoma cells to a well-differentiated state. A copy of HALF1 is found in high-molecular-weight DNA in the human genome. The genomic equivalent of HALF1 and its flanking sequences [gH(5'-3') fragment]...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1180
更新日期:1994-07-01 00:00:00