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Growth of intestinal epithelium in organ culture is dependent on EGF signalling.

Abstract：

:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with the selective apoptosis of superficial cells lining the lumen while cell proliferation is progressively restricted to cells adjacent to the basement membrane. We describe an innovative embryonic gut culture system that maintains the three-dimensional architecture of gut and in which these processes are recapitulated in vitro. Explants taken from specific regions of the gut and placed into organ culture develop and express molecular markers (Cdx1, Cdx2 and A33 antigen) in the same spatial and temporal pattern observed in vivo indicating that regional specification is maintained. Inhibition of the epidermal growth factor receptor (EGFR) tyrosine kinase using the specific inhibitor AG1478 significantly reduced the proliferation and survival of cells within the epithelial cell layer of cultured gut explants. This demonstrates an essential role for the EGF signalling pathway during the early stages of intestinal development.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Abud HE,Watson N,Heath JK

doi

10.1016/j.yexcr.2004.10.006

keywords:

subject

Has Abstract

pub_date

2005-02-15 00:00:00

pages

252-62

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(04)00584-1

journal_volume

303

pub_type

杂志文章

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