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CHO cell aggregation induced by fibronectin-coated beads. Differences between wild-type and adhesion-variant cells.

Abstract：

:ADvF11 (F11), a Chinese Hamster Ovary (CHO) cell variant, is defective in its ability to adhere to fibronectin (Fn)-coated substrata but will adhere to substrata coated with poly-L-lysine, conA or extracellular matrix (ECM) [1]. We have observed that both F11 and CHO wild-type (WT) cells were able to bind 3H-Fn beads in a similar manner; however, only WT cells and not F11 cells aggregate in the presence of Fn beads. Both cell types aggregated similarly in the presence of lectins. Fn-bead-mediated aggregation was blocked by low temperature and aggregation did not occur when formaldehyde-fixed WT cells were used. Colchicine, tetracaine and cytochalasin B were not effective in blocking aggregation induced by Fn beads. These results suggest that: 1. Both WT and F11 cells have surface membrane-binding sites for Fn. 2. The aggregation defect in F11 cells is distal to the initial interaction between the cell surface and Fn, but proximal to the cytoskeletal rearrangements required for cell adhesion.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Cheung E,Juliano RL

doi

10.1016/0014-4827(84)90236-2

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

127-33

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(84)90236-2

journal_volume

152

pub_type

杂志文章

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