当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Head-to-head association in bovine spermatozoa induced by catecholamines.

Head-to-head association in bovine spermatozoa induced by catecholamines.

Abstract：

:Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+, oxidation products of adrenaline dissociated spermatozoa associated by washing; at 2 X 10(-5) M Ca2+, the dissociating effect was transformed into association. The induction of association by adrenaline was blocked by both alpha- and beta-adrenergic blockers at low concentrations (2 X 10(-7) M). Both cAMP and dibutyryl substituted cAMP (db-cAMP) induced association in the Tris-buffered medium at 2 X 10(-6) M Ca2+. Further increase in association was brought about by increasing the Ca2+ concentration to 2 X 10(-5) M. Prolongation of the treatment with cAMP increased association. When combined with cAMP under the same conditions as used in the combination with adrenaline, L-propranolol did not inhibit association induced by cAMP. In an identical experiment, performed in Tyrode solution, L-propranolol inhibited association induced by cAMP. At 2 X 10(-5) M, theophylline, caffeine, and papaverine induced association in the presence of 2 X 10(-5) M Ca2+. The results are compatible with the hypothesis that catecholamines act via receptors and formation of cAMP.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Lindahl PE

doi

10.1016/0014-4827(78)90384-1

subject

Has Abstract

pub_date

1978-05-01 00:00:00

pages

421-33

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

113

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Oxysterol-binding protein-related protein (ORP) 6 localizes to the ER and ER-plasma membrane contact sites and is involved in the turnover of PI4P in cerebellar granule neurons.

    abstract：:Oxysterol-binding protein (OSBP)-related proteins (ORPs) are conserved lipid binding proteins found in organisms ranging from yeast to mammals. Recent findings have indicated that these proteins mainly localize to contact sites of 2 different membranous organelles. ORP6, a member of the ORP subfamily III, is one of th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.07.025

    authors： Mochizuki S,Miki H,Zhou R,Kido Y,Nishimura W,Kikuchi M,Noda Y

    更新日期：2018-09-15 00:00:00

  • Homeostatic regulation of meiotic DSB formation by ATM/ATR.

    abstract：:Ataxia-telangiectasia mutated (ATM) and RAD3-related (ATR) are widely known as being central players in the mitotic DNA damage response (DDR), mounting responses to DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) respectively. The DDR signalling cascade couples cell cycle control to damage-sensing and ...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2014.07.016

    authors： Cooper TJ,Wardell K,Garcia V,Neale MJ

    更新日期：2014-11-15 00:00:00

  • Insulin binds to type V collagen with retention of mitogenic activity.

    abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90351-t

    authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

    更新日期：1991-06-01 00:00:00

  • TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties.

    abstract：:The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knoc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.05.007

    authors： Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

    更新日期：2011-07-15 00:00:00

  • The protective effect of hispidin against hydrogen peroxide-induced apoptosis in H9c2 cardiomyoblast cells through Akt/GSK-3β and ERK1/2 signaling pathway.

    abstract：:Hispidin, a phenolic compound from Phellinus linteus (a medicinal mushroom), has been shown to possess strong anti-oxidant, anti-cancer, anti-diabetic, and anti-dementia properties. However, the cardioprotective efficacy of hispidin has not yet been investigated. In the present study, we investigated the protective ef...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.07.037

    authors： Kim DE,Kim B,Shin HS,Kwon HJ,Park ES

    更新日期：2014-10-01 00:00:00

  • Identification of a nuclear export signal in the KSHV latent protein LANA2 mediating its export from the nucleus.

    abstract：:LANA2 is a latent protein detected in Kaposi's sarcoma-associated herpesvirus (KSHV)-infected B cells that inhibits p53-dependent transcriptional transactivation and apoptosis and PKR-dependent apoptosis, suggesting an important role in the transforming activity of the virus. It has been reported that LANA2 localizes ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.08.022

    authors： Muñoz-Fontela C,Collado M,Rodriguez E,García MA,Alvarez-Barrientos A,Arroyo J,Nombela C,Rivas C

    更新日期：2005-11-15 00:00:00

  • Microtubule-mediated NF-kappaB activation in the TNF-alpha signaling pathway.

    abstract：:The microtubule cytoskeleton is known to play a role in cell structure and serve as a scaffold for a variety of active molecules in processes as diverse as motility and cell division. The literature on the role of microtubules in signal transduction, however, is marked by inconsistencies. We have investigated a well-s...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.08.020

    authors： Jackman RW,Rhoads MG,Cornwell E,Kandarian SC

    更新日期：2009-11-15 00:00:00

  • Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators.

    abstract：:Deregulation of the bone morphogenetic protein (BMP) pathway has been documented in colorectal cancer (CRC). Previously, we investigated possible associations between urokinase-type plasminogen activator (uPA) deficiency and expression of extracellular constituents of BMP signaling in a newly developed mouse model of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.10.025

    authors： Afaloniati H,Karagiannis GS,Hardas A,Poutahidis T,Angelopoulou K

    更新日期：2017-12-15 00:00:00

  • Complement 5a stimulates macrophage polarization and contributes to tumor metastases of colon cancer.

    abstract：:Inflammatory cells such as macrophages can play a pro-tumorigenic role in the tumor stroma. Tumor-associated macrophages (TAMs) generally display an M2 phenotype with tumor-promoting activity; however, the mechanisms regulating the TAM phenotype remain unclear. Complement 5a (C5a) is a cytokine-like polypeptide that i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.03.009

    authors： Piao C,Zhang WM,Li TT,Zhang CC,Qiu S,Liu Y,Liu S,Jin M,Jia LX,Song WC,Du J

    更新日期：2018-05-15 00:00:00

  • Rhodamine 123 uptake and mitochondrial DNA content in rabbit articular chondrocytes evolve differently upon transfer from cartilage to culture conditions.

    abstract：:Mitochondrial DNA (mtDNA) represents 0.15% of the total cell DNA (at least an order of magnitude less than in liver or heart) of rabbit articular chondrocytes. Besides the already well-documented low respiratory activity, chondrocyte differentiation thus involves a specific control of mitochondrial biogenesis. When tr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90172-8

    authors： Champagne AM,Benel L,Ronot X,Mignotte F,Adolphe M,Mounolou JC

    更新日期：1987-08-01 00:00:00

  • Galectin-3 stimulates cell proliferation.

    abstract：:Galectin-3, a beta-galactoside-binding protein, has been shown to be involved in multiple biological processes through interaction with its complementary glycoconjugates. Here we provide the first evidence of galectin-3 as a mitogen. Incubation of quiescent cultures of normal human lung fibroblast IMR-90 cells with re...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4253

    authors： Inohara H,Akahani S,Raz A

    更新日期：1998-12-15 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract：:A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90173-x

    authors： Moore SA

    更新日期：1987-08-01 00:00:00

  • Parathyroid hormone-related peptide (PTHrP) inhibits Runx2 expression through the PKA signaling pathway.

    abstract：:The bone-related transcription factor Runx2 (Cbfa1) has been extensively shown to regulate osteoblast differentiation and function. Recent studies demonstrate that Runx2 is also a positive regulator of chondrocyte maturation and vascular invasion in cartilage. Runx2 activity can be modulated in several ways, including...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.05.025

    authors： Li TF,Dong Y,Ionescu AM,Rosier RN,Zuscik MJ,Schwarz EM,O'Keefe RJ,Drissi H

    更新日期：2004-09-10 00:00:00

  • High levels of the molecular chaperone Mdg1/ERdj4 reflect the activation state of endothelial cells.

    abstract：:Mdg1/ERdj4, a mammalian chaperone that belongs to the HSP40 protein family, has been reported to be located in the endoplasmic reticulum (ER), is induced by ER stress, and protects ER stressed cells from apoptosis. Here we show that under normal physiological conditions, Mdg1/ERdj4 is expressed at various levels in th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00316-1

    authors： Berger BJ,Müller TS,Buschmann IR,Peters K,Kirsch M,Christ B,Pröls F

    更新日期：2003-10-15 00:00:00

  • Establishment of a germ-line competent C57BL/6 embryonic stem cell line.

    abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90430-3

    authors： Ledermann B,Bürki K

    更新日期：1991-12-01 00:00:00

  • Increment of nonreceptor tyrosine kinase Arg RNA as evaluated by semiquantitative RT-PCR in granulocyte and macrophage-like differentiation of HL-60 cells.

    abstract：:The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a m...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4234

    authors： Perego RA,Bianchi C,Brando B,Urbano M,Del Monte U

    更新日期：1998-11-25 00:00:00

  • Epigenetic activation of the 5-hydroxytryptamine (serotonin) receptor 2C in embryonal carcinoma cells is DNA replication-dependent.

    abstract：:The epigenetic states of key regulatory genes must be altered to drive cell fate decisions in differentiating cells. This process must be coupled, at least transiently, to the DNA replication machinery. Only a few genes, however, have been shown to require DNA replication for their activation or repression upon induct...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.04.021

    authors： Bancescu DL,Glatt-Deeley H,Lalande M

    更新日期：2004-08-01 00:00:00

  • Complex formation between tissue transglutaminase II (tTG) and vascular endothelial growth factor receptor 2 (VEGFR-2): proposed mechanism for modulation of endothelial cell response to VEGF.

    abstract：:We have recently demonstrated that thrombin-activated FXIII (FXIIIA-subunit), a plasma transglutaminase, activates VEGFR-2 by crosslinking it with the alpha(v)beta(3) integrin on the surface of endothelial cells (EC), thereby stimulating angiogenesis. Tissue transglutaminase (tTG), which is functionally and structural...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.05.019

    authors： Dardik R,Inbal A

    更新日期：2006-10-01 00:00:00

  • Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleus.

    abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90583-5

    authors： Nakamura H,Morita T,Sato C

    更新日期：1986-08-01 00:00:00

  • The different roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by human fibroblasts.

    abstract：:Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fiber...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.04.013

    authors： Liu Z,Ho CH,Grinnell F

    更新日期：2014-08-15 00:00:00

  • Cytostatic factor (CSF) activity in cytosols extracted from Xenopus laevis eggs.

    abstract：:Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90211-r

    authors： Moses RM,Masui Y

    更新日期：1990-01-01 00:00:00

  • The structural analysis of adhesions mediated by Ep-CAM.

    abstract：:The epithelial cell adhesion molecule Ep-CAM is capable of mediating Ca2+-independent homotypic cell-cell adhesion when introduced into cells lacking their own means of cell-cell interactions. We used (confocal) immunofluorescent and (immuno-) electron microscopy to investigate the structural organization of Ep-CAM-me...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4263

    authors： Balzar M,Prins FA,Bakker HA,Fleuren GJ,Warnaar SO,Litvinov SV

    更新日期：1999-01-10 00:00:00

  • The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules.

    abstract：:Sertoli cells of testis belong to a unique type of polarized epithelial cells and are essential for spermatogenesis. They form the blood-testis barrier at the base of seminiferous tubule. Their numerous long, microtubule-rich processes extend inward and associate with developing germ cells to sustain germ cell growth ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.09.022

    authors： Wang F,Zhang Q,Cao J,Huang Q,Zhu X

    更新日期：2008-01-01 00:00:00

  • Hedgehog pathway plays a vital role in HIV-induced epithelial-mesenchymal transition of podocyte.

    abstract：:HIV-associated nephropathy (HIVAN) is characterized by heavy proteinuria, rapidly progressive renal failure, and distinct morphological features in the kidney. HIV-induced epithelial-mesenchymal transition (EMT) is critically important for the progression of kidney injury. In this study, we tested the role of hedgehog...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.01.019

    authors： Lan X,Wen H,Cheng K,Plagov A,Marashi Shoshtari SS,Malhotra A,Singhal PC

    更新日期：2017-03-15 00:00:00

  • The Ca2+ signaling capacity of the beta 2-integrin on HL60-granulocytic cells is abrogated following phosphorylation of its CD18-chain: relation to impaired protein tyrosine phosphorylation.

    abstract：:The phosphorylation state of the CD18-chain of beta 2-integrins have been shown not to mediate changes in the avidity of these receptors (i.e., inside-out signaling); however, no alternative functional significance has been proposed. Our study focused on how changes in the phosphorylation state of beta 2-integrin-rece...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1073

    authors： Hellberg C,Eierman D,Sjölander A,Andersson T

    更新日期：1995-03-01 00:00:00

  • AKT upregulates B-Raf Ser445 phosphorylation and ERK1/2 activation in prostate cancer cells in response to androgen depletion.

    abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.008

    authors： Hong SK,Jeong JH,Chan AM,Park JI

    更新日期：2013-07-15 00:00:00

  • The ING1b tumor suppressor facilitates nucleotide excision repair by promoting chromatin accessibility to XPA.

    abstract：:ING1b is the most studied ING family protein and perhaps the most ubiquitously and abundantly expressed. This protein is involved in the regulation of various biological functions ranging from senescence, cell cycle arrest, apoptosis, to DNA repair. ING1b is upregulated by UV irradiation and enhances the removal of bu...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.02.010

    authors： Kuo WH,Wang Y,Wong RP,Campos EI,Li G

    更新日期：2007-05-01 00:00:00

  • Autoimmune regulator is acetylated by transcription coactivator CBP/p300.

    abstract：:The Autoimmune Regulator (AIRE) is a regulator of transcription in the thymic medulla, where it controls the expression of a large set of peripheral-tissue specific genes. AIRE interacts with the transcriptional coactivator and acetyltransferase CBP and synergistically cooperates with it in transcriptional activation....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.04.013

    authors： Saare M,Rebane A,Rajashekar B,Vilo J,Peterson P

    更新日期：2012-08-15 00:00:00

  • TRIM24 promotes hepatocellular carcinoma progression via AMPK signaling.

    abstract：:Hepatocellular carcinoma (HCC) is one of the most common cancers diagnosed worldwide. However, the mechanism underlying HCC pathogenesis remains unknown. In the present study, TRIM24 was found increased in human HCC clinical samples and positively correlated with HCC tumor grade. Furthermore, TRIM24 knockdown inhibits...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.04.006

    authors： Zhu Y,Zhao L,Shi K,Huang Z,Chen B

    更新日期：2018-06-15 00:00:00

  • Murine embryonal carcinoma cell-surface sialyl LeX is present on a novel glycoprotein and on high-molecular-weight lactosaminoglycan.

    abstract：:Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90061-x

    authors： Rosenman SJ,Fenderson BA,Hakomori SI

    更新日期：1989-02-01 00:00:00