当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Activity of a novel PDGF beta-receptor enhancer during the cell cycle and upon differentiation of neuroblastoma.

Activity of a novel PDGF beta-receptor enhancer during the cell cycle and upon differentiation of neuroblastoma.

Abstract：

:PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF beta-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFRB gene. They were partially digested with TspI and cloned into the PDGFRB enhancer trap vector to make a library for identification of enhancers. The digested DNA containing enhancer was identified by expression of GFP when transfected in PDGF beta-receptor expressing cells. One of the enhancer clones was further examined by making several deletion mutants in a luciferase vector. This enhancer was most active in neuroblastoma cells, IMR32 and BE2, but less active in hemangioma and in smooth muscle cell lines. Chip assay revealed that SP1, AP2, and GATA2 bound the enhancer in BE2 cells. Their interaction occurred dependently of the cell cycle and synchronously with their binding to the promoter. Transfection of GATA2 alone or with Ets, which binds adjacent to GATA, resulted in differentiation of BE2 cells in parallel with increased PDGF beta-receptor expression. Furthermore, over-expression of the PDGF beta-receptor in BE2 cells induced neurite extension.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Kaneko M,Yang W,Matsumoto Y,Watt F,Funa K

doi

10.1016/j.yexcr.2006.03.005

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

2028-39

issue

11

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(06)00089-9

journal_volume

312

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Macrophage characteristics of stem cells revealed by transcriptome profiling.

    abstract：:We previously showed that the phenotypes of adipocyte progenitors and macrophages were close. Using functional analyses and microarray technology, we first tested whether this intriguing relationship was specific to adipocyte progenitors or could be shared with other progenitors. Measurements of phagocytic activity an...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.06.034

    authors： Charrière GM,Cousin B,Arnaud E,Saillan-Barreau C,André M,Massoudi A,Dani C,Pénicaud L,Casteilla L

    更新日期：2006-10-15 00:00:00

  • Stable intermediates and holdpoints in the rapid differentiation of Naegleria.

    abstract：:The rapidity of the optional 90-min differentiation of Naegleria gruberi from amoebae to flagellates suggests the possibility of a free-running cascade of events from initiating stimulus through gene expression to organelle assembly and cell morphogenesis. Instead our experiments reveal two points early in the differe...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4121

    authors： Fulton C,Lai EY

    更新日期：1998-08-01 00:00:00

  • CD21lo/medCD27+ proinflammatory B cells are enriched in breast cancer patients and promote antitumor T cell responses.

    abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.10.013

    authors： Zhu S,Wang X,Wang J,Lin J,Cong Y,Qiao G

    更新日期：2017-12-01 00:00:00

  • Keratocytes are induced to produce collagen type II: A new strategy for in vivo corneal matrix regeneration.

    abstract：:The stroma, the middle layer of the cornea, is a connective tissue making up most of the corneal thickness. The stromal extracellular matrix (ECM) consists of highly organised lamellae which are made up of tightly packed fibrils primarily composed of collagens type I and V. This layer is interspersed with keratocytes,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.08.010

    authors： Greene CA,Green CR,Dickinson ME,Johnson V,Sherwin T

    更新日期：2016-09-10 00:00:00

  • Thrombin-mast cell interactions. Binding and cell activation.

    abstract：:Activation of mouse bone marrow-derived mast cells (BMMC) by thrombin (0.05-0.5 U/million cells) resulted in a concentration-dependent release of histamine, which levelled off by 0.1 U thrombin. Rat peritoneal mast cells (RMC) were not stimulated by thrombin, though in control experiments, both types of mast cells deg...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90184-3

    authors： Razin E,Baranes D,Marx G

    更新日期：1985-10-01 00:00:00

  • p66Shc promotes HCC progression in the tumor microenvironment via STAT3 signaling.

    abstract：:The development of hepatocellular carcinoma (HCC) is strongly associated with chronic inflammation. p66Shc is an oxidase previously shown to promote androgen-independent cell growth through generation of reactive oxygen species. However, the importance and biologic functions of p66Shc in HCC are unclear. The clinical ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111550

    authors： Huang P,Feng X,Zhao Z,Yang B,Fang T,Guo M,Xia J

    更新日期：2019-10-15 00:00:00

  • A lipoxygenase metabolite, 12-(S)-HETE, stimulates protein kinase C-mediated release of cathepsin B from malignant cells.

    abstract：:The process of tumor cell invasion of the basement membrane is proposed to consist of three steps: attachment, local proteolysis and migration. 12-(S)-HETE, a 12-lipoxygenase metabolite of arachidonic acid, upregulates surface expression of integrin cytoadhesins and an autocrine motility factor receptor, suggesting th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1240

    authors： Honn KV,Timár J,Rozhin J,Bazaz R,Sameni M,Ziegler G,Sloane BF

    更新日期：1994-09-01 00:00:00

  • Tetracyclines cause cell stress-dependent ATF4 activation and mTOR inhibition.

    abstract：:Tetracyclines have long been used as valuable broad-spectrum antibiotics. The high antibacterial activity of tetracyclines, combined with their good tolerability, has led to their widespread use in treating various infectious diseases. However, similar to other antibiotics, tetracyclines are also known for their adver...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.11.012

    authors： Brüning A,Brem GJ,Vogel M,Mylonas I

    更新日期：2014-01-15 00:00:00

  • Reversion of bovine papillomavirus-induced transformation and immortalization by a xanthate compound.

    abstract：:Bovine papilloma virus-transformed hamster embryo fibroblasts (HEF-BPV) reacted to exposure to tricyclodecan-9-yl-xanthogenate (D609) with immediate reversion to the growth kinetics and the flat morphology of the untransformed parental cells. After six population doublings in the presence of D609, clones which display...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90109-0

    authors： Amtmann E,Müller K,Knapp A,Sauer G

    更新日期：1985-12-01 00:00:00

  • A functional role for ERK in gene induction, but not in neurite outgrowth in differentiating neuroblastoma cells.

    abstract：:The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5163

    authors： Olsson AK,Nånberg E

    更新日期：2001-04-15 00:00:00

  • Senescence stimulates U937-endothelial cell interactions.

    abstract：:Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial mono...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1246

    authors： Maier JA,Statuto M,Ragnotti G

    更新日期：1993-09-01 00:00:00

  • Control of apoptosis and growth of malignant T lymphoma cells by lymph node stromal cells.

    abstract：:We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underw...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1193

    authors： Kataoka S,Naito M,Fujita N,Ishii H,Ishii S,Yamori T,Nakajima M,Tsuruo T

    更新日期：1993-08-01 00:00:00

  • Cdc25A localisation and shuttling: characterisation of sequences mediating nuclear export and import.

    abstract：:The Cdc25 phosphatases play crucial roles in cell cycle progression by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Cdc25A is an important regulator of the G1/S transition but functions also in the mitotic phase of the human cell cycle. In this paper, we investigate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.09.012

    authors： Källström H,Lindqvist A,Pospisil V,Lundgren A,Rosenthal CK

    更新日期：2005-02-01 00:00:00

  • The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor.

    abstract：:Activation of growth factor receptors, such as platelet-derived growth factor (PDGF) receptors, has a major impact on the motile behavior of vertebrate cells. The WASP family of proteins has been recognized as important regulators of actin polymerization via the activation of the Arp2/3 complex. The activity of the WA...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5576

    authors： Aspenström P

    更新日期：2002-09-10 00:00:00

  • S632A3, a new glutarimide antibiotic, suppresses lipopolysaccharide-induced pro-inflammatory responses via inhibiting the activation of glycogen synthase kinase 3β.

    abstract：:Inflammatory mediators including inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) contribute to the course of a variety of inflammatory diseases. S632A3 is a new member of the glutarimide antibiotics isolated from a cultured broth of Streptomyces hygrosc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.08.008

    authors： Deng H,Zhang N,Wang Y,Chen J,Shen J,Wang Z,Xu R,Zhang J,Song D,Li D

    更新日期：2012-12-10 00:00:00

  • SNEV overexpression extends the life span of human endothelial cells.

    abstract：:In a recent screening for genes down regulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.11.025

    authors： Voglauer R,Chang MW,Dampier B,Wieser M,Baumann K,Sterovsky T,Schreiber M,Katinger H,Grillari J

    更新日期：2006-04-01 00:00:00

  • Trefoil peptides promote restitution of wounded corneal epithelial cells.

    abstract：:The ocular surface shares many characteristics with mucosal surfaces. In both, healing is regulated by peptide growth factors, cytokines, and extracellular matrix proteins. However, these factors are not sufficient to ensure most rapid healing. Trefoil peptides are abundantly expressed epithelial cell products which e...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5116

    authors： Göke MN,Cook JR,Kunert KS,Fini ME,Gipson IK,Podolsky DK

    更新日期：2001-04-01 00:00:00

  • Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice.

    abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.12.026

    authors： Deutsch U,Schlaeger TM,Dehouck B,Döring A,Tauber S,Risau W,Engelhardt B

    更新日期：2008-04-01 00:00:00

  • The MAPK ERK5, but not ERK1/2, inhibits the progression of monocytic phenotype to the functioning macrophage.

    abstract：:Intracellular signaling pathways present targets for pharmacological agents with potential for treatment of neoplastic diseases, with some disease remissions already recorded. However, cellular compensatory mechanisms usually negate the initial success. For instance, attempts to interrupt aberrant signaling downstream...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.10.003

    authors： Wang X,Pesakhov S,Harrison JS,Kafka M,Danilenko M,Studzinski GP

    更新日期：2015-01-01 00:00:00

  • Doubling of cell mass is not necessary in order to achieve cell division in cultured human cells.

    abstract：:When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90788-2

    authors： Rønning OW,Pettersen EO

    更新日期：1984-11-01 00:00:00

  • Glycerol and polylysine synergize in their ability to rupture vesicular membranes: a mechanism for increased transferrin-polylysine-mediated gene transfer.

    abstract：:The presence of about 1.2 M glycerol during transfection with DNA/transferrin-polylysine and DNA/polylysine complexes dramatically increases transgene expression in a variety of cell types, provided that the complexes have an excess of polylysine. We have characterized this phenomenon using a human melanoma cell line ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3486

    authors： Zauner W,Kichler A,Schmidt W,Mechtler K,Wagner E

    更新日期：1997-04-10 00:00:00

  • Cell-substrate interaction. A method for evaluating the possible correlation between metastatic phenotype and cell surface energy.

    abstract：:The interfacial energy of the non-attached to substrate cell surface was analysed in tumor cell variants of the K-1735 melanoma and UV-2237 fibrosarcoma series, which exhibit distinct metastatic phenotypes. The highly metastatic cell variants exhibited a two-fold increase in the ability to form rapid cell substrate in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90625-6

    authors： Reich S,Rosin H,Levy M,Karkash R,Raz A

    更新日期：1984-08-01 00:00:00

  • Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes.

    abstract：:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0123

    authors： Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

    更新日期：1996-04-10 00:00:00

  • Tenascin-C in the extracellular matrix promotes the selection of highly proliferative and tubulogenesis-defective endothelial cells.

    abstract：:The extracellular matrix (ECM) contains important cues for tissue homeostasis and morphogenesis. The matricellular protein tenascin-C (TN-C) is overexpressed in remodeling tissues and cancer. In the present work, we studied the effect of different ECM-which exhibited a significant diversity in their TN-C content-in en...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.06.006

    authors： Alves TR,da Fonseca AC,Nunes SS,da Silva AO,Dubois LG,Faria J,Kahn SA,Viana NB,Marcondes J,Legrand C,Moura-Neto V,Morandi V

    更新日期：2011-09-10 00:00:00

  • Lamin dynamics during sea urchin male pronuclear formation in vitro.

    abstract：:The dynamics of lamin disassembly and reassembly during sea urchin male pronuclear development in vitro was investigated. Using five anti-lamin antibodies, we monitored by immunofluorescence and immunoblotting the changes in lamins during sperm chromatin decondensation, nuclear envelope (NE) formation, and male pronuc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1280

    authors： Collas P,Pinto-Correia C,Poccia DL

    更新日期：1995-08-01 00:00:00

  • Nucleocytoplasmic shuttling of STK16 (PKL12), a Golgi-resident serine/threonine kinase involved in VEGF expression regulation.

    abstract：:PKL12/STK16 protein is the first identified mammalian member of a ser/thr kinase subfamily that is conserved across several kingdoms, with a broad expression pattern in murine tissues and cell types. Endogenous STK16 subcellular localization was evaluated by indirect immunofluorescence in NIH/3T3 and NRK cells, demons...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.10.010

    authors： Guinea B,Ligos JM,Laín de Lera T,Martín-Caballero J,Flores J,Gonzalez de la Peña M,García-Castro J,Bernad A

    更新日期：2006-01-15 00:00:00

  • TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties.

    abstract：:The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knoc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.05.007

    authors： Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

    更新日期：2011-07-15 00:00:00

  • Differences in tyrosine phosphorylation of oocyte key proteins during 5HT-induced meiosis reinitiation in two bivalve species.

    abstract：:The neurohormone serotonin (5HT) triggers meiosis reinitiation in oocytes of the pelecypod molluscs Spisula solidissima and Ruditapes philippinarum. However, while Spisula oocytes complete maturation, Ruditapes oocytes arrest in metaphase I. Anti-phosphotyrosine antibody revealed that serotonin triggered an early tyro...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1163

    authors： Abdelmajid H,Rivailler P,Krantic S,Guerrier P

    更新日期：1994-06-01 00:00:00

  • Insulin suppresses distal-less homeobox 5 expression through the up-regulation of microRNA-124 in 3T3-L1 cells.

    abstract：:Distal-less homeobox 5 (Dlx5) is a pro-osteogenic but anti-adipogenic transcription factor that regulates lineage commitment in mesenchymal stem cells. Although the expression of Dlx5 is known to be decreased by adipogenic stimuli, the mechanism of Dlx5 down-regulation has not yet been clarified. MicroRNAs (miRNAs) ar...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.04.020

    authors： Qadir AS,Woo KM,Ryoo HM,Baek JH

    更新日期：2013-08-15 00:00:00

  • Focal adhesion kinase pp125FAK and the beta 1 integrin subunit are constitutively complexed in HaCaT cells.

    abstract：:Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possib...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3916

    authors： Danker K,Gabriel B,Heidrich C,Reutter W

    更新日期：1998-03-15 00:00:00