An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat fibroblast line 3Y1.

Abstract:

:A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutation. We then isolated rat D123 cDNA from a 3Y1 cDNA library using the human D123 cDNA probe. Both human and rat D123 cDNA contained a long open-reading-frame coding for a novel protein (designated as D123 protein) of 336 amino acids. A comparison of the nucleotide sequences of rat D123 cDNA and the corresponding cDNA of 3Y1tsD123-obtained by polymerase chain reaction in conjunction with reverse transcription showed that there was a difference in a single nucleotide that must cause a single amino acid change in D123 protein. Antibody raised against the recombinant protein of human D123 cDNA detected a 44-kDa D123 protein in human embryo lung fibroblasts and 3Y1. The level of D123 protein was much lower in 3Y1tsD123 than in 3Y1 at both permissive (33.8 degrees C) and restrictive (39.8 degrees C) temperatures. We suppose that quantitative and qualitative changes of D123 protein due to the amino acid change cause the temperature-sensitive arrest of 3Y1tsD123. On the other hand, the level of D123 protein in 3Y1 did not change significantly whether cells were proliferating or arrested at the saturation cell density. This indicates that the G1 arrest of 3Y1 at the saturation cell density does not involve the control of D123 protein level.

journal_name

Exp Cell Res

authors

Okuda A,Kimura G

doi

10.1006/excr.1996.0078

subject

Has Abstract

pub_date

1996-03-15 00:00:00

pages

242-9

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(96)90078-6

journal_volume

223

pub_type

杂志文章
  • Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation: TNF-alpha inhibits commitment.

    abstract::We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(02)00036-8

    authors: Castro-Muñozledo F,Beltrán-Langarica A,Kuri-Harcuch W

    更新日期:2003-04-01 00:00:00

  • Reconstitution of dna synthetic capacity in senescent normal human fibroblasts by expressing cellular factors E2F and Mdm2.

    abstract::Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenoviru...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5352

    authors: Sarraj S,Farb R,Martell RE

    更新日期:2001-11-01 00:00:00

  • Cellular localization and expression of template-activating factor I in different cell types.

    abstract::Template-activating factors I (TAF-I) alpha and beta have been identified as chromatin remodeling factors from human HeLa cells. TAF-I beta corresponds to the protein encoded by the set gene, which was found in an acute undifferentiated leukemia as a fusion version with the can gene via chromosomal translocation. To d...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3930

    authors: Nagata K,Saito S,Okuwaki M,Kawase H,Furuya A,Kusano A,Hanai N,Okuda A,Kikuchi A

    更新日期:1998-05-01 00:00:00

  • Glycerol and polylysine synergize in their ability to rupture vesicular membranes: a mechanism for increased transferrin-polylysine-mediated gene transfer.

    abstract::The presence of about 1.2 M glycerol during transfection with DNA/transferrin-polylysine and DNA/polylysine complexes dramatically increases transgene expression in a variety of cell types, provided that the complexes have an excess of polylysine. We have characterized this phenomenon using a human melanoma cell line ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3486

    authors: Zauner W,Kichler A,Schmidt W,Mechtler K,Wagner E

    更新日期:1997-04-10 00:00:00

  • Inhibition of miRNA-21 promotes retinal ganglion cell survival and visual function by modulating Müller cell gliosis after optic nerve crush.

    abstract:BACKGROUND:Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environme...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.01.009

    authors: Li HJ,Sun ZL,Pan YB,Sun YY,Xu MH,Feng DF

    更新日期:2019-02-15 00:00:00

  • Increment of nonreceptor tyrosine kinase Arg RNA as evaluated by semiquantitative RT-PCR in granulocyte and macrophage-like differentiation of HL-60 cells.

    abstract::The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a m...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4234

    authors: Perego RA,Bianchi C,Brando B,Urbano M,Del Monte U

    更新日期:1998-11-25 00:00:00

  • Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.

    abstract::The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. In the reproductive system, despite Gsα being associated with oocyte meiotic arres...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2018.07.023

    authors: Xie Y,Wu B,Jin Y,Zhang A,Sun X,Zhang X,Gao X,Dong R,Li H,Gao J

    更新日期:2018-09-15 00:00:00

  • Vascular endothelial growth factor promotes cardiac stem cell migration via the PI3K/Akt pathway.

    abstract::VEGF is a major inducer of angiogenesis. However, the homing role of VEGF for cardiac stem cells (CSCs) is unclear. In in vitro experiments, CSCs were isolated from the rat hearts, and a cellular migration assay was performed using a 24-well transwell system. VEGF induced CSC migration in a concentration-dependent man...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2009.09.026

    authors: Tang J,Wang J,Kong X,Yang J,Guo L,Zheng F,Zhang L,Huang Y,Wan Y

    更新日期:2009-12-10 00:00:00

  • Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.

    abstract::Since the migration of human fetal skin fibroblasts into a denuded area of a cell monolayer has been demonstrated to be serum-independent (fetal phenotype), whereas that of adult-donor skin fibroblasts is serum-dependent (adult phenotype), a study was carried out to determine at which life stage the fetal-adult phenot...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1342

    authors: Kondo H,Yonezawa Y

    更新日期:1995-10-01 00:00:00

  • Antiapoptotic effect of human T-cell leukemia virus type 1 tax protein correlates with its creb transcriptional activity.

    abstract::Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.01.009

    authors: Trevisan R,Daprai L,Paloschi L,Vajente N,Chieco-Bianchi L,Saggioro D

    更新日期:2006-05-01 00:00:00

  • Migrational changes of mesenchymal stem cells in response to cytokines, growth factors, hypoxia, and aging.

    abstract::Mesenchymal stem cells (MSCs) are non-immunogenic, multipotent cells with at least trilineage differentiation potential. They promote wound healing, improve regeneration of injured tissue, and mediate numerous other health effects. MSCs migrate to sites of injury and stimulate repair either through direct differentiat...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.08.019

    authors: Naaldijk Y,Johnson AA,Ishak S,Meisel HJ,Hohaus C,Stolzing A

    更新日期:2015-10-15 00:00:00

  • Downregulation of endogenous STAT3 augments tumoricidal activity of interleukin 15 activated dendritic cell against lymphoma and leukemia via TRAIL.

    abstract::Effector functions in tumor resistance by dendritic cells (DCs) are less well characterized. In this study, we describe that the murine DCs upon stimulation with recombinant IL-15 in vitro or in vivo, expresses TNF superfamily member TRAIL which mediates cytotoxicity and growth inhibition against a murine lymphoma cal...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2014.08.012

    authors: Hira SK,Mondal I,Bhattacharya D,Manna PP

    更新日期:2014-10-01 00:00:00

  • Coronin 3 involvement in F-actin-dependent processes at the cell cortex.

    abstract::The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.12.015

    authors: Rosentreter A,Hofmann A,Xavier CP,Stumpf M,Noegel AA,Clemen CS

    更新日期:2007-03-10 00:00:00

  • CXCR4 and CXCL12 are inversely expressed in colorectal cancer cells and modulate cancer cell migration, invasion and MMP-9 activation.

    abstract::Colorectal cancer (CRC) is characterized by a distinct metastatic pattern resembling chemokine-induced leukocyte trafficking. This prompted us to investigate expression, signal transduction and specific functions of the chemokine receptor CXCR4 in CRC cells and metastases. Using RT-PCR analysis and Western blotting, w...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2005.07.006

    authors: Brand S,Dambacher J,Beigel F,Olszak T,Diebold J,Otte JM,Göke B,Eichhorst ST

    更新日期:2005-10-15 00:00:00

  • Protective effect of N-acetylcysteine in tumor necrosis factor-alpha-induced apoptosis in U937 cells: the role of mitochondria.

    abstract::The existence of two different pathways for cell death has been postulated. In addition to the passive and traumatic process leading to necrosis, an active program characterized by organelle integrity and called apoptosis has been described. A positive correlation between the apoptotic cell death process and oxidative...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1311

    authors: Cossarizza A,Franceschi C,Monti D,Salvioli S,Bellesia E,Rivabene R,Biondo L,Rainaldi G,Tinari A,Malorni W

    更新日期:1995-09-01 00:00:00

  • Sequence determinants for hnRNP I protein nuclear localization.

    abstract::hnRNP I, also referred to as polypyrimidine tract binding protein, is one of the proteins associated with nascent pre-mRNA in the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. As for all karyophilic proteins, the nuclear import of hnRNP proteins requires specific sequence determinants that in many instanc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3677

    authors: Romanelli MG,Weighardt F,Biamonti G,Riva S,Morandi C

    更新日期:1997-08-25 00:00:00

  • Differentiation-linked expression of prothymosin alpha gene in human myeloid leukemic cells.

    abstract::Prothymosin alpha (ProT alpha) is a nuclear protein related to cell proliferation. Its gene is highly activated during postnatal development at stages containing many proliferating but also differentiating cells. In this report, a study on ProT alpha gene expression during differentiation of human myeloid leukemic (HL...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1013

    authors: Dosil M,Alvarez-Fernández L,Gómez-Márquez J

    更新日期:1993-01-01 00:00:00

  • Induction of human high K(M) 5'-nucleotidase in cultured 293 cells.

    abstract::Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4681

    authors: Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi V

    更新日期:1999-12-15 00:00:00

  • Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.

    abstract::On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90062-5

    authors: Ohno T,Kako R,Sato C,Ohkawa A

    更新日期:1986-04-01 00:00:00

  • Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1 proteins during Fcgamma-receptor-mediated phagocytosis in alveolar macrophages.

    abstract::Recent studies have demonstrated a central role for the exchange protein activated by cAMP (Epac) in the inhibition of Fcgamma-receptor-mediated phagocytosis and bacterial killing by prostaglandin E(2) (PGE(2)) in macrophages. However, the subcellular localization of Epac, and its primary target Rap1, has yet to be de...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.10.011

    authors: Brock TG,Serezani CH,Carstens JK,Peters-Golden M,Aronoff DM

    更新日期:2008-01-15 00:00:00

  • Cell attachment and spreading on extracellular matrix-coated beads.

    abstract::Parietal yolk sac cells M1536 -B3 grown on cytodex 2 beads deposited an extracellular matrix on the surface of the beads. Cell-free matrix-coated beads were isolated by treatment of the cell monolayer with cytochalasin B (CB) at a concentration of 10 micrograms/ml of phosphate-buffered saline (PBS). The matrix when an...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90652-9

    authors: Mai S,Chung AE

    更新日期:1984-06-01 00:00:00

  • Complex formation between tissue transglutaminase II (tTG) and vascular endothelial growth factor receptor 2 (VEGFR-2): proposed mechanism for modulation of endothelial cell response to VEGF.

    abstract::We have recently demonstrated that thrombin-activated FXIII (FXIIIA-subunit), a plasma transglutaminase, activates VEGFR-2 by crosslinking it with the alpha(v)beta(3) integrin on the surface of endothelial cells (EC), thereby stimulating angiogenesis. Tissue transglutaminase (tTG), which is functionally and structural...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.05.019

    authors: Dardik R,Inbal A

    更新日期:2006-10-01 00:00:00

  • Factors controlling the appearance of the immunofluorescent nuclear dots revealed with monoclonal antibody against microtubule-associated protein-1.

    abstract::A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transf...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90480-x

    authors: Ohno T,Ohkawa A,Sato C

    更新日期:1985-06-01 00:00:00

  • Purification and characterization of integrin alpha 9 beta 1.

    abstract::A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cyst...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1994.1189

    authors: Forsberg E,Ek B,Engström A,Johansson S

    更新日期:1994-07-01 00:00:00

  • Effects of succinylacetone on dimethylsulfoxide-mediated induction of heme pathway enzymes in mouse friend virus-transformed erythroleukemia cells.

    abstract::Heme has been reported to exert a control over its own biosynthesis and to affect the erythroid differentiation process at different sites. In this study, succinylacetone, a powerful inhibitor of delta-aminolevulinic acid dehydrase was used to block heme synthesis and to study the effects of heme depletion on the dime...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90171-x

    authors: Beaumont C,Deybach JC,Grandchamp B,da Silva V,de Verneuil H,Nordmann Y

    更新日期:1984-10-01 00:00:00

  • The antioxidant, rather than prooxidant, activities of quercetin on normal cells: quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis.

    abstract::The bioflavonoid quercetin is a dietary anticancer chemical that is capable of inducing apoptosis in tumor cells. Although the activity of quercetin is believed to be due to its antioxidative properties, it has recently been suggested that quercetin also has prooxidant activities, which might effect cytotoxicity direc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(03)00410-5

    authors: Lee JC,Kim J,Park JK,Chung GH,Jang YS

    更新日期:2003-12-10 00:00:00

  • Phorbol ester- and calcium-induced reorganization of 180-kDa bullous pemphigoid antigen on the ventral surface of cultured human keratinocytes as studied by immunofluorescence and immunoelectron microscopy.

    abstract::The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(92)90034-6

    authors: Kitajima Y,Owaribe K,Nishizawa Y,Jokura Y,Yaoita H

    更新日期:1992-11-01 00:00:00

  • Protein kinase calpha is an effector of hexamethylene bisacetamide-induced differentiation of Friend erythroleukemia cells.

    abstract::The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4312

    authors: Mallia CM,Aguirre V,McGary E,Tang Y,Scandurro AB,Liu C,Noguchi CT,Beckman BS

    更新日期:1999-02-01 00:00:00

  • Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro.

    abstract::The formation of capillaries during development and tissue repair is likely to involve active reorganization of the actin cytoskeleton, although few studies have addressed this issue. Here, we have utilized an in vitro model of capillary morphogenesis whereby human umbilical vein endothelial cells are suspended within...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4460

    authors: Salazar R,Bell SE,Davis GE

    更新日期:1999-05-25 00:00:00

  • Production of trypsin by cells of the exocrine pancreas is paralleled by the expression of the KH protein vigilin.

    abstract::Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3891

    authors: Kruse C,Emmrich J,Rumpel E,Klinger MH,Grünweller A,Rohwedel J,Krammer HJ,Kühnel W,Müller PK

    更新日期:1998-02-25 00:00:00